Betulinic acid and alphitolic acid, the triterpenoids of Zyziphi Fructus, were isolated with silica gel column chromatography and used as the standard substances for the analysis. The compounds were determined with HPLC and HPTLC. They were separated ...
Betulinic acid and alphitolic acid, the triterpenoids of Zyziphi Fructus, were isolated with silica gel column chromatography and used as the standard substances for the analysis. The compounds were determined with HPLC and HPTLC. They were separated on reversed phase coumn (Nova-Pak C_18) with 0.05 M Na_2HPO_4-methanol (19:81) in HPLC and detected at 210 ㎚. Separation on HPTLC precoated sillica gel F_254 plates was carried out with chloroform-methanol (6:1) and the separated compounds were reacted with ρ-anisaldehyde and detected at 540 ㎚. The contents of betulinic acid and alphitolic acid in Zyziphi Fructus from four different regions in Korea were in the range of 2.9~3.8% and 3.2~3.9%, respectively.