Production of Polyhydroxybutyrate from Crude Glycerol and Spent Coffee Grounds Extract by Bacillus cereus Isolated from Sewage Treatment Plant

다국어 초록 (Multilingual Abstract)

Production of biodegradable polymer polyhydroxyalkanoates(PHAs) from industrial wastes exhibits severaladvantages such as recycle of waste and the production ofhigh valuable products. To this end, this study aimed at isolatingfrom the sewage treatment plant a PHA producing bacteriumcapable of utilizing wastes generated from biodiesel andfood industries. A Bacillus cereus strain capable of producingpoly(3-hydroxybutyrate) [P(3HB)] was isolated, which wasfollowed by confirmation of P(3HB) accumulation by gaschromatographicanalyses. Then, the effects of nutrient limitationon P(3HB) production by B. cereus was first examined.
Cells cultured in a minimal medium under the limitation of nitrogen,potassium and sulfur suggested that nitrogen limitationallows the highest P(3HB) accumulation. Next, production ofP(3HB) was examined from both waste of biodiesel production(crude glycerol) and waste from food industry (spent coffeegrounds). Cells cultured in nitrogen-limited minimalmedium supplemented crude glycerol and waste spent coffeegrounds extract accumulated P(3HB) to the contents of 2.4%and 1.0% of DCW. This is the first report demonstrating thecapability of B. cereus to produce P(3HB) from waste rawmaterials such as crude glycerol and spent coffee grounds.

참고문헌 (Reference)

1 Duke, J. A., "The handbook of plant science in agriculture" CRC Press, Inc 1984

2 Johnson, D. T., "The glycerin glut: Options for the value-added conversion of crude glycerol resulting from biodiesel production" 26 : 338-348, 2007

3 Kondamudi, N., "Spent coffee grounds as a versatile source of green energy" 56 : 11757-11760, 2008

4 Lee, S. Y., "Production of medium-chain-length polyhydroxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitation" 68 : 466-470, 2000

5 Mothes, G., "Production of PHB from crude glycerol" 7 : 475-479, 2007

6 Greenspan, P., "Nile red: a selective fluorescent stain for intracellular lipid droplets" 100 : 965-973, 1985

7 Mu, Y., "Microbial production of 1,3-propanediol by Klebsiella pneumoniae using crude glycerol from biodiesel preparations" 28 : 1755-1759, 2006

8 Park, S. J., "Metabolic engineering of Ralstonia eutropha for the biosynthesis of 2-hydroxyacid-containing polyhydroxyalkanoates." 20 : 20-28, 2013

9 Jung, Y. K., "Metabolic engineering of Escherichia coli for the production of polylactic acid and its copolymers" 105 : 161-171, 2010

10 Tamura, K., "MEGA6: Molecular evolutionary genetics analysis version 6.0" 30 : 2725-2729, 2013

11 Reddy, S. V., "Isolation of bacteria producing polyhydroxyalkanoates (PHA) from municipal sewage sludge" 24 : 2949-2955, 2008

12 Jacquel, N., "Isolation and purification of bacterial poly(3-hydroxyalkanoates)" 39 : 15-27, 2008

13 Sohn, S. B., "In silico genome-scale metabolic analysis of Pseudomonas putida KT2440for polyhydroxyalkanoate synthesis, degradation of aromatics and anaerobic survival" 5 : 739-750, 2010

14 Sabourin-Provost, G., "High yield conversion of a crude glycerol fraction from biodiesel production to hydrogen by photofermentation" 100 : 3513-3517, 2009

15 Malaviya, A., "Continuous butanol production with reduced byproducts formation from glycerol by a hyper producing mutant of Clostridium pasteurianum" 93 : 1485-1494, 2012

16 Bertrand, B., "Comparison of bean biochemical composition and beverage quality of Arabica hybrids involving Sudanese-Ethiopian origins with traditional varieties at various elevations in Central America" 26 : 1239-1248, 2006

17 Hu, S., "Characterization of crude glycerol from biodiesel plants" 60 : 5915-5921, 2012

18 Zhang, H. F., "Biosynthesis and characterization of 3-hydroxyalkanoate terpolyesters with adjustable properties by Aeromonas hydrophila" 104 : 582-589, 2009

19 Corma, A., "Biomass to chemicals: Catalytic conversion of glycerol/water mixtures into acrolein, reaction network" 257 : 163-171, 2008

20 Arya, M., "An impression of coffee carbohydrates" 47 : 51-67, 2007

21 Park, S. J., "Advanced bacterial polyhydroxyalkanoates: towards a versatile and sustainable platform for unnatural tailor-made polyesters" 30 : 1196-1206, 2012

22 Braunegg, G., "A rapid gas chromatographic method for the determination of poly-b-hydroxybutyric acid in microbial biomass" 6 : 29-37, 1978