프로폴리스의 생물학적 특성

국문 초록 (Abstract)

프로폴리스는 꿀벌에 의해 생산된 수지 물질로 벌집을 보호하기 위해 사용되어 왔다. 꿀벌은 다양한 나무와 식물에서 배출된 수분을 침과 밀랍과 섞어서 프로폴리스를 생산한다. 프로폴리스는 기원전 300년경부터 상처를 치료하는 민간요법으로 사용되어 왔다. 프로폴리스에는 플라보노이드, 페놀계 화합물, 밀랍과 같은 많은 생리 활성 화합물이 포함되어 있다. 프로폴리스의 기능적 요소들 때문에, 프로폴리스에는 생물학적 응용을 위한 넓은 스펙트럼이 있다. 프로폴리스와 프로폴리스의 생물학적 활동의 화합물은 각각 과즙의 출처와 추출 방법에 따라 달라질 수 있다. 프로폴리스의 박테리아, 바이러스, 곰팡이를 포함한 반미생물 활동이 가장 잘 알려진 특징이다. 면역조절 메커니즘의 경우 아르테필린 C와 카페인산 페네틸에스테르가 주로 조절 화합물로 확인되었으며 각 화합물은 염증 반응을 감소시키고 T 림프구에 면역억제 반응을 일으킨다. 프로폴리스는 항염증 효과를 통해 암세포 증식 억제, 종양 신호 전달 캐스케이드 차단, 항혈관신생 등 항종양 활성을 보였다. 그러나 프로폴리스의 더 많은 응용을 위해서는 과즙의 원천 분석, 프로폴리스 화합물의 식별, 프로폴리스 분자 메커니즘, 화합물 시너지 효과의 조사가 더 필요할 것으로 보인다.

다국어 초록 (Multilingual Abstract)

Propolis is a resinous substance produced by honeybees, which they use to protect their hives. Honeybees produce propolis by mixing exudates from the various trees and plants with saliva and beeswax. It has been used since around 300 B.C. as a folk medicine to cure wounds. Propolis contains many physiologically active components, such as flavonoids, phenolic compounds, and beeswax. Because of its functional components, propolis has a wide spectrum of biological applications. The compounds in propolis and its biological activity can vary according to the location of nectar source and extraction method. Propolis is most commonly known for its anti-microorganism activity against bacteria, viruses, and fungi. Artepillin C and caffeic acid phenethyl ester (CAPE) have been identified as regulatory compounds that reduce inflammation and exert immunosuppressive reactions on T lymphocytes. Through its anti-inflammatory activity, propolis exhibits anti-tumor activity, including the inhibition of cancer cell proliferation, the blocking of tumor signaling cascades, and antiangiogenesis. However, for the more apply of propolis its analysis of nectar source, identifying of propolis compound, the molecular mechanism of propolis and the investigation of compounds synergistic effects are essential. In this study, we described the physiological activity of propolis isolated from honeybees.

목차 (Table of Contents)

• 서론
• 본론
• 결론
• References
• 초록

참고문헌 (Reference)

1 정일윤, "프로폴리스에서 분리한 플라보노이드 화합물의 항산화 활성 및 방사선 방어효과" 한국식품영양과학회 34 (34): 162-166, 2005

2 김세건, "지역별 국산 프로폴리스의 항균활성 및 Pinocembrin의 UPLC 분석" 한국양봉학회 32 (32): 253-259, 2017

3 우순옥, "국내산 프로폴리스의 총플라보노이드 및 총페놀함량 비교" 한국양봉학회 30 (30): 293-298, 2015

4 Luceri, C., "p-Coumaric acid, a common dietary phenol, inhibits platelet activity in vitro and in vivo" 97 : 458-463, 2007

5 Fung, K. L., "Vincristine but not imatinib could suppress mesenchymal niche's support to lymphoid leukemic cells" 51 : 515-522, 2009

6 Cole, N., "Topical ‘Sydney’ propolis protects against UV-radiation-induced inflammation, lipid peroxidation and immune suppression in mouse skin" 152 : 87-97, 2010

7 Padmavathi, R., "Therapeutic effect of paclitaxel and propolis on lipid peroxidation and antioxidant system in 7, 12 dimethyl benz(a)anthracene-induced breast cancer in female Sprague Dawley rats" 78 : 2820-2825, 2006

8 Sze, D. M., "The use of thalidomide in myeloma therapy as an effective anticancer drug" 6 : 325-331, 2006

9 Seeley, T. D., "The nest of the honeybee(Apis mellifera)" 23 : 495-512, 1976

10 Hosnuter, M., "The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury" 30 : 121-125, 2004

11 Grassi, D., "Tea, flavonoids, and nitric oxide-mediated vascular reactivity" 138 : 1554-, 2008

12 Li, H. Q., "Synthesis of C(7)modified chrysin derivatives designing to inhibit beta-ketoacyl-acyl carrier protein synthase III(FabH)as antibiotics" 17 : 6264-6269, 2009

13 Orsolic, N., "Synergistic antitumor effect of polyphenolic components of water soluble derivative of propolis against Ehrlich ascites tumour" 28 : 694-700, 2005

14 Bag, A., "Synergistic antibacterial and antibiofilm efficacy of nisin in combination with p-coumaric acid against food-borne bacteria Bacillus cereus and Salmonella typhimurium" 65 : 366-372, 2017

15 Ahn, M. R., "Suppression of tumor-induced angiogenesis by Brazilian propolis : major component artepillin C inhibits in vitro tube formation and endothelial cell proliferation" 252 : 235-243, 2007

16 Lee, S. W., "Studies on the flavonoid compositions of Korean propolis" 21 : 389-394, 2001

17 Veljkovic, V., "Simple criterion for selection of flavonoid compounds with anti-HIV activity" 17 : 1226-1232, 2007

18 Sforcin, J. M., "Seasonal effect on Brazilian propolis anti-bacterial activity" 73 : 243-249, 2000

19 Shengxi Meng, "Roles of Chlorogenic Acid on Regulating Glucose and Lipids Metabolism: A Review" Hindawi Limited 2013 : 1-11, 2013

20 Burdock, G. A., "Review of the biological properties and toxicity of bee propolis(propolis)" 36 : 347-363, 1998

21 Metodiewa, D., "Reactive oxygen species and reactive nitrogen species: relevance to cyto(neuro)toxic events and neurologic disorders. An overview" 1 : 197-233, 2000

22 van Raamsdonk, J. M., "Reactive oxygen species and aging in Caenorhabditis elegans : causal or casual relationship?" 12 : 1911-1953, 2010

23 Fialkow, L, "Reactive oxygen and nitrogen species as signaling molecules regulating neutrophil function" 42 : 153-164,

24 Powers, S. K., "Reactive oxygen and nitrogen species as intracellular signals in skeletal muscle" 589 : 2129-2138, 2011

25 김성국, "Raw264.7 대식세포에서 국산 프로폴리스 추출물에 의한 항염증 효과" 한국양봉학회 33 (33): 187-194, 2018

26 Pandey, A. K., "Quercetin in hypoxia-induced oxidative stress : novel target for neuroprotection" 102 : 107-146, 2012

27 Remirez, D., "Protective effects of propolis extract on allyl alcohol-induced liver injury in mice" 4 : 309-314, 1997

28 Orsolic, N., "Protective effects of propolis and related polyphenolic/flavonoid compounds against toxicity induced by irinotecan" 27 : 1346-1358, 2009

29 Kolankaya, D., "Protective effects of Turkish propolis on alcohol-induced serum lipid changes and liver injury in male rats" 78 : 213-217, 2002

30 Bazo, A. P., "Protective action of propolis on the rat colon carcinogenesis" 22 : 183-194, 2002

31 Imhof, M., "Propolis solution for the treatment of chronic vaginitis" 89 : 127-132, 2005

32 Capoci, I. R. G., "Propolis is an efficient fungicide and inhibitor of biofilm production by vaginal Candida albicans" 2015 : 287693-, 2015

33 Jain, S., "Propolis in oral health : a natural remedy" 2 : 90-94, 2014

34 Ansorge, S., "Propolis and some of its constituents down-regulate DNA synthesis and inflammatory cytokine production but induce TGF-1production of human immune cells" 58 : 580-589, 2003

35 de Groot, A. C., "Propolis : a review of properties, applications, chemical composition, contact allergy, and other adverse effects" 24 : 263-282, 2013

36 Ramos, A. F. N., "Propolis : a review of its anti-inflammatory and healing actions" 13 : 697-710, 2007

37 Ghisalberti, E. L., "Propolis : a review" 60 : 59-84, 1979

38 Aliyazicioglu Y., "Preventive and protective effects of Turkish propolis on H2O2 induced DNA damage in foreskin fibroblast cell lines" 62 : 388-396, 2011

39 Rasul, A., "Pinocembrin : a novel natural compound with versatile pharmacological and biological activities" 2013 : 379850-, 2013

40 Bankova, V., "Phytochemical evidence for the plant origin of Brazilian propolis from Sao Paulo state" 54 : 401-405, 1999

41 Marcucci, M. C., "Phenolic compounds from Brazilian propolis with pharmacological activities" 74 : 105-112, 2001

42 Cooke, M. S., "Oxidative DNA damage : mechanisms, mutation, and disease" 17 : 1195-1214, 2003

43 Salatino, A., "Origin and chemical variation of Brazilian propolis" 2 : 33-38, 2005

44 Mansouri, M. T., "Neuroprotective effects of oral gallic acid against oxidative stress induced by 6-hydroxydopamine in rats" 138 : 1028-1033, 2013

45 Ramesh, E., "Naringin induces death receptor and mitochondria-mediated apoptosis in human cervical cancer(SiHa)cells" 51 : 97-105, 2013

46 Sahu, B. D., "Naringin ameliorates gentamicin-induced nephrotoxicity and associated mitochondrial dysfunction, apoptosis and inflammation in rats : possible mechanism of nephroprotection" 277 : 8-20, 2014

47 de Lima, R. O., "Modifying effect of propolis on dimethylhydrazine-induced DNA damage but not colonic aberrant crypt foci in rats" 45 : 8-16, 2005

48 Khayyal, M. T., "Mechanisms involved in the anti-inflammatory effect of propolis extract" 19 : 197-203, 1993

49 Tekin, I. O., "Low-density lipoproteins oxidized after intestinal ischemia/reperfusion in rats" 157 : e47-e54, 2009

50 Hattori, H., "Isolation, identification, and biological evaluation of HIF-1-modulating compounds from Brazilian green propolis" 19 : 5392-5401, 2011

51 Krol, W., "Inhibition of nitric oxide (NO.)production in murine macrophages by flavones" 50 : 1031-1035, 1995

52 Fesen, M. R., "Inhibition of HIV-1 integrase by flavones, caffeic acid phenethyl ester(CAPE)and related compounds" 48 : 595-608, 1994

53 Gorinstein, S., "Influence of two cultivars of persimmon on atherosclerosis indices in rats fed cholesterol-containing diets : Investigation in vitro and in vivo" 27 : 838-846, 2011

54 Libby, P., "Inflammation in atherosclerosis" 32 : 2045-2051, 2012

55 Falcao, S. I., "In vitro evaluation of Portuguese propolis and floral sources for antiprotozoal, antibacterial and antifungal activity" 28 : 437-443, 2014

56 Freitas, S., "In vitro effects of propolis on Giardia duodenalis trophozoites" 13 : 170-175, 2006

57 Moriyasu, J., "In vitro activation of mouse macrophage by propolis extract powder" 8 : 364-365, 1994

58 Khlebnikov, A. I., "Improved quantitative structure-activity relationship models to predict antioxidant activity of flavonoids in chemical, enzymatic, and cellular systems" 15 : 1749-1770, 2007

59 Dimov, V., "Immunomodulatory action of propolis. Influence on anti-infections protection and macrophage function" 22 : 155-162, 1991

60 Orsi, R. O., "Immunomodulatory action of propolis on macrophage activation" 6 : 205-219, 2000

61 Benjamin, E. J., "Heart disease and stroke statistics—2017 update : a report from the American Heart Association" 135 : e146-, 2017

62 Tomar, A., "Galangin ameliorates cisplatin induced nephrotoxicity in vivo by modulation of oxidative stress, apoptosis and inflammation through interplay of MAPK signaling cascade" 34 : 154-161, 2017

63 Viuda-Martos, M., "Functional properties of honey, propolis, and royal jelly" 73 : R117-R124, 2008

64 Xi, W., "Flavonoid composition and antioxidant activities of Chinese local pummelo (Citrus grandis Osbeck.) varieties" 161 : 230-238, 2014

65 Cunha, I. B. S., "Factors that influence the yield and composition of Brazilian propolis extracts" 15 : 964-970, 2004

66 Iio, A., "Ethanolic extracts of Brazilian red propolis increase ABCA1 expression and promote cholesterol efflux from THP-1 macrophages" 19 : 383-388, 2012

67 Yu, Y., "Ethanolic extract of propolis promotes reverse cholesterol transport and the expression of ATP-binding cassette transporter A1 and G1 in mice" 46 : 805-811, 2011

68 Song, Y. S., "Estrogenic effects of ethanol and ether extracts of propolis" 82 : 89-95, 2002

69 Mello, A. P. Q., "Electronegative low-density lipoprotein : origin and impact on health and disease" 215 : 257-265, 2011

70 Fuliang, H. U., "Effects of propolis on blood glucose, blood lipid and free radicals in ratswithdiabetesmellitus" 51 : 147-152, 2005

71 Yajing Li, "Effects of Encapsulated Propolis on Blood Glycemic Control, Lipid Metabolism, and Insulin Resistance in Type 2 Diabetes Mellitus Rats" Hindawi Limited 2012 : 1-8, 2012

72 Shad, B., "Effect of multimorbidity on quality of life in adult with cardiovascular disease : A cross-sectional study" 15 : 1-8, 2017

73 Kawase, A., "Differential effects of chrysin on nitrofurantoin pharmacokinetics mediated by intestinal breast cancer resistance protein in rats and mice" 12 : 150-163, 2009

74 Pereira, E. M. R., "Clinical evidence of the efficacy of a mouthwash containing propolis for the control of plaque and gingivitis : a phase II study" 2011 : 750249-, 2011

75 Jang, H., "Chlorogenic acid and coffee prevent hypoxia-induced retinal degeneration" 62 : 182-191, 2014

76 Russo, A., "Chilean propolis : antioxidant activity and antiproliferative action in human tumor cell lines" 76 : 545-558, 2004

77 Fang, D., "Chemopreventive mechanisms of galangin against hepatocellular carcinoma : A review" 109 : 2054-2061, 2019

78 Machado, B. A. S., "Chemical composition and biological activity of extracts opbtained by supercritical extraction and ethanolic extraction of brown, green and red propolis derived from different geographic regions in Brazil" 11 : e0145954-, 2016

79 Waller, S. B., "Chemical and cytotoxic analyses of brown Brazilian propolis(Apis mellifera)and its in vitro activity against itraconazole-resistant Sporothrix brasiliensis" 105 : 117-121, 2017

80 Albukhari, A. A., "Caffeic acid phenethyl ester protects against tamoxifen-induced hepatotoxicity in rats" 47 : 1689-1695, 2009

81 Kart, A., "Caffeic acid phenethyl ester prevents ovary ischemia/reperfusion injury in rabbits" 47 : 1980-1984, 2009

82 Lee, K. W., "Caffeic acid phenethyl ester inhibits invasion and expression of matrix metalloproteinase in SK-Hep1 human hepatocellular carcinoma cells by targeting nuclear factor kappa B" 2 : 319-322, 2008

83 Sudina, G. F., "Caffeic acid phenethyl ester as a lipoxygenase inhibitor with antioxidant properties" 329 : 21-24, 1993

84 Omene, C. O., "Caffeic Acid Phenethyl Ester(CAPE)derived from propolis, a honeybee product, inhibits growth of breast cancer stem cells" 30 : 1279-1288, 2011

85 Demestre, M., "CAPE(caffeic acid phenethyl ester)-based propolis extract(Bio 30)suppresses the growth of human neurofibromatosis(NF)tumor xenografts in mice" 23 : 226-230, 2009

86 Dalia, G., "Burden of cardiovascular disease (CVD) on economic cost. Comparison of outcomes in US and Europe" 10 : A207-, 2017

87 Righi, A. A., "Brazilian red propolis : unreported substances, antioxidant and antimicrobial activities" 91 : 2363-2370, 2011

88 Kunimasa, K., "Brazilian propolis suppresses angiogenesis by inducing apoptosis in tube-forming endothelial cells through inactivation of survival signal ERK1/2" 2011 : 870753-, 2011

89 Cheung, K. W., "Brazilian green propolis and its constituent, artepillin C inhibits allogeneic activated human CD4 T cells expansion and activation" 138 : 463-471, 2011

90 de Freitas, M. C. D., "Biological activities of red propolis : A review" 11 : 3-12, 2017

91 Izuta, H., "Bee products prevent VEGF-induced angiogenesis in human umbilical vein endothelial cells" 9 : 2009

92 Messerli, S. M., "Artepillin C(ARC)in Brazilian green propolis selectively blocks oncogenic PAK1 signaling and suppresses the growth of NF tumors in mice" 23 : 423-427, 2009

93 Alday, E., "Apoptotic induction by pinobanksin and some of its ester derivatives from Sonoran propolis in a B-cell lymphoma cell line" 242 : 35-44, 2015

94 Khoo, B. Y., "Apoptotic effects of chrysin in human cancer cell lines" 11 : 2188-2199, 2010

95 Kimoto, T., "Apoptosis of human leukemia cells induced by Artepillin C, an active ingredient of Brazilian propolis" 21 : 221-228, 2001

96 Su, Z. Z., "Apoptosis mediates the selective toxicity of caffeic acid phenethyl ester(CAPE)toward oncogene-transformed rat embryo fibroblast cells" 15 : 1841-1848, 1995

97 Schnitzler, P., "Antiviral activity and mode of action of propolis extracts and selected compounds" 24 : S20-S28, 2010

98 Paulino, N., "Antiulcerogenic effect of Brazilian propolis formulation in mice" 6 : 580-, 2015

99 Xuan, H., "Antitumor activity of Chinese propolis in human breast cancer MCF-7 and MDA-MB-231 cells" 2014 : 280120-, 2014

100 Ledon, N., "Antipsoriatic, anti-inflammatory, and analgesic effects of an extract of red propolis" 18 : 274-276, 1997

101 Demir, S., "Antiproliferative and proapoptotic activity of Turkish propolis on human lung cancer cell line" 68 : 165-172, 2016

102 Chen, T. G., "Antiplatelet activity of caffeic acid phenethyl ester is mediated through a cyclic GMP-dependent pathway in human platelets" 50 : 121-126, 2007

103 Choi, Y. H., "Antioxidant vitamins and magnesium and the risk of hearing loss in the US general population" 99 : 148-155, 2014

104 Abbasi-Parizad, P., "Antioxidant and anti-inflammatory activities of the crude extracts of raw and fermented tomato pomace and their correlations with aglycate-polyphenols" 9 : 179-, 2020

105 Silva, V., "Antioxidant activity of Uruguayan propolis" 59 : 6430-6437, 2011

106 Isla, M. I., "Antioxidant activity of Argentine propolis extracts" 76 : 165-170, 2001

107 Bertolini, P. F. R., "Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes : an in vitro study" 20 : 32-37, 2012

108 Mirzoeva, O. K., "Antimicrobial action of propolis and some of its components : the effects on growth, membrane potential and motility of bacteria" 152 : 239-246, 1997

109 Dobrowolski, J. W., "Antibacterial, antifungal, antiamoebic, antiinflammatory and antipyretic studies on propolis bee products" 35 : 77-82, 1991

110 Daleprane, J. B., "Antiatherogenic and anti-angiogenic activities of polyphenols from propolis" 23 : 557-566, 2012

111 Norata, G. D., "Anti-inflammatory and anti-atherogenic effects of cathechin, caffeic acid and trans-resveratrol in apolipoprotein E deficient mice" 191 : 265-271, 2007

112 Almeida, E. C., "Anti-inflammatory activity of propolis extracts : a review" 8 : 191-212, 2002

113 Chikaraishi, Y., "Angiostatic effects of Brazilian green propolis and its chemical constituents" 54 : 566-575, 2009

114 Aysel Ugur, "An In Vitro Study on Antimicrobial Activity of Propolis from Mugla Province of Turkey" 한국식품영양과학회 7 (7): 90-94, 2004

115 Benguedouar, L., "Algerian ethanolic extract of propolis and galangin decreased melanoma tumour progression in C57BL6 mice" 16 : 1172-1183, 2015

116 Visscher, P., "Adaptations of honey bees(Apis mellifera)to problems of nest hygiene" 5 : 249-260, 1980

117 Said, R. A., "Absence of chemopreventive influence of propolis on the rat liver altered foci development" 62 : 405-412, 2010

118 Liu, H., "(2R, 3S)-Pinobanksin-3-cinnamate improves cognition and reduces oxidative stress in rats with vascular dementia" 69 : 358-365, 2015