Bifidobacteriurn longum, Bifidobacterium adolescentis, Clostridium butyricum, Escherichia coli and Eubacterium limosum were cultured on the modified PYF medium containing 0.5% of glucose, heating extract(w/w) of Angelica acutiloba or Glycyrrhiza urale...
Bifidobacteriurn longum, Bifidobacterium adolescentis, Clostridium butyricum, Escherichia coli and Eubacterium limosum were cultured on the modified PYF medium containing 0.5% of glucose, heating extract(w/w) of Angelica acutiloba or Glycyrrhiza uralensis, and anaerobically incubated for 48hrs at 37℃. As a control, the same intestinal microorganisms cultured on the PYF medium with no additives and they were compared with other treatments. The addition of the heating extract of Angelica acutiloba increase the growth but optical density was lower than addition of glucose for the growth of intestinal microorgamisms which were investigated in this study. However, the heating extract of Glycyrrhiza uralensis slightly inhibited the growth for most intestinal microorganisms(Bif. longum, Bif. adolescentis, Cl. butyricum, and Eu. limosum) and it had a significant inhibition effect on the growth of Eu limosum when they were compared with control.