An ELISA (Enzyme Linked Immunosorbent Assay) method was studied for rapid and sensitive detection of entomocidal toxins of Bacillus thuringiensis subsp. kurstaki and israelensis, potent microbial insecticides. Crystalline toxins were purified with a T...
An ELISA (Enzyme Linked Immunosorbent Assay) method was studied for rapid and sensitive detection of entomocidal toxins of Bacillus thuringiensis subsp. kurstaki and israelensis, potent microbial insecticides. Crystalline toxins were purified with a Telebrix double layer density centrifuge and antisera were prepared from rabbits, respectively. Using these antisera, both indirect and sandwich ELISA methods were examined and compared. Both ELISA systems were demonstrated to be sensitive and specific for the respectable toxins. The minimum detectable concentrations of toxins were about 2-10 ng toxin/㎖.