Application of Bacillus pumilus b-xylosidase reaction and simulated moving bed purification to efficient production of high-purity xylobiose from xylose

다국어 초록 (Multilingual Abstract)

Xylobiose (X2) is recognized to possess great prebiotic function and to be highly favorable for applicationin food and prebiotic industries. In this study, we demonstrated that the cloned b-xylosidase of Bacilluspumilus IPO could be utilized to produce X2 via the reaction of xylose(X1)!xylobiose(X2). The use ofsuch enzyme in the X1!X2 reaction was found to give much higher X2 reaction yield and reactionefficiency, compared to those reported in the literature. Furthermore, we developed an efficientsimulated moving bed (SMB) chromatographic process that could recover X2 from the reaction outputwith nearly 100% purity and 92% recovery on a continuous-separation mode. The developed SMB processcould also recover the unreacted X1 almost completely, which leaves room for a further increase in theoverall X2 reaction yield by reusing the recovered X1 from the SMB as the reactant of the upstreamprocessing (i.e., B. pumilus IPO b-xylosidase X1!X2 reaction). The results of this study will enable ahighly economical and environmentally-friendly production of high-purity X2 from X1.

참고문헌 (Reference)

1 Z. Ma, 43 : 2488-, 1997

2 R. B. Kasat, 27 : 1785-, 2003

3 K. J. Jeong, 22 : 599-, 1998

4 L. C. Kebler, 1207 : 55-, 2008

5 K. B. Lee, 54 : 2852-, 2008

6 J. S. Mackie, 232 : 498-, 1955

7 M. J. Vazquez, 11 : 387-, 2000

8 Q. P. Yuan, 79 : 1073-, 2004

9 M. Okazaki, 43 : 395-, 1990

10 M. Okazaki, 9 : 77-, 1990

11 M. D. Howard, 125 : 2604-, 1995

12 P. Moura, 40 : 963-, 2007

13 P. Katapodis, 41 : 2402-, 2006

14 M. Guerfali, 150 : 267-, 2008

15 J. S. Hur, 45 : 1426-, 2006

16 A. G. O'Brien, 51 : 7028-, 2012

17 J. W. Lee, 25 : 355-, 2014

18 H. Moriyama, 166 : 539-, 1987

19 W. Z. Xu, 202 : 1197-, 1991

20 W. Panbangred, 192 : 335-, 1983

21 K. Terpe, 72 : 211-, 2006

22 K. Wagschal, 155 : 304-, 2009

23 Ł. Wi sniewski, 20 : 483-, 2014

24 J. H. Choi, 1465 : 143-, 2016

25 C. Y. Chin, 33 : 77-, 2004

26 S. F. Chung, 14 : 857-, 1968

27 E. J. Wilson, 5 : 9-, 1966

28 C. R. Wilke, 1 : 264-, 1955

29 P.C. Wankat, "Rate-Controlled Separations" Blackie Academic & Professional 1994

30 Y. Suwa, "Bifidobacterium bifidum proliferation promoting composition containing xylooligosaccharide, U.S Pat. 5939309"

31 송성문, "A three-zone simulated moving-bed for separation of immunoglobulin Y" 한국화학공학회 30 (30): 1527-1532, 2013