Effects of Nitrogen Concentration on Root Respiration Rate and Nonstructural Carbohydrates of Walnut Seedlings

다국어 초록 (Multilingual Abstract)

Nitrogen is a critical determinant of forest growth and productivity, and root respiration rate and nonstructural carbohydrates are important parameters for evaluating the effects of supplied nutrients. Here, we assessed the effects of nitrogen on walnut seedlings (Juglans regia Linn.) that grew consistently for a year under favorable conditions. Seedlings were potted in seven concentrations of N availability, 0, 1, 2, 4, 8, 12, and 16 mmol·L1, and the specific root respiration rate and nonstructural carbohydrate content of the root system were determined via the offbody root system and the ketone color ratio methods, respectively. Nitrogen concentration was positively correlated with specific root respiration, and root diameter was the main factor related to specific root respiration and nonstructural carbohydrate content of walnut seedlings. Under the same nitrogen concentrations, the soluble total sugar and starch content of roots increased with increasing root diameter, while the specific root respiration increased with decreasing root diameter. Compared to the 0 mmol·L1 nitrogen concentration, the content of nonstructural carbohydrates in the root system and the specific root respiration were higher when nitrogen concentration was N16. Our comprehensive analysis showed that a nitrogen concentration of N16 improved the respiration rate of the seedling root system, significantly increased the content of nonstructural carbohydrates in the seedling root system, and effectively promoted the growth and development of the seedling root system of walnut seedlings.

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