페닐보론산을 포함하는 역오팔 수화젤 포도당센서의 제작 = Fabrication of inverse opal hydrogel glucose sensor containing phenylboronic acid

다국어 초록 (Multilingual Abstract)

There is growing interest in continuous glucose monitoring systems (CGMS) for diabetes patients, and inverse opal photonic gels (IOPG), IOPG is expected to be the core technology of CGMS because it can observe blood glucose level by reversible reflective color change without power supply units . In this study, we demonstrate a glucose sensing hydrogel which can observe blood glucose at physiological pH was prepared by intramolecular charge stabilization of phenylboronate by quaternary amine, and hydrogel was fabricated by using PBA with fluorine for improving sensitivity. Using 2-Hydroxyethyl methacrylate (HEMA) as inverse opal photonic hydrogel main building block monomer, (3-Acrylamidopropyl)trimethylammonium chloride (APTA) and 2-(methacryloyloxy)ethyltrimethylammonium chloride (MOETA) as two different charge stabilizer were, were copolymerized with 3-acrylamidophenyl boronic acid (3-AAPBA) which can detected glucose by reversible binding reaction between glucose and boronate. Also, acrylamide (AAm) and 3-fluoro-4-acrylamido-phenylboronic acid (3F4AAPBA) were copolymerized for the purpose of improving sensitivity. The hydrogel was fabricated by Directed Enhanced Evaporative colloidal assembly (DEECA) method. The inverse opal photonic hydrogel, including APTA or MOETA, showed increased sensitivity at pH 7.4, which is physiological pH. The inverse opal photonic hydrogel containing 3F4AAPBA were further improved in sensitivity and hydrogel showed red, green, and blue reflection color at physiological blood glucose levels.

국문 초록 (Abstract)

당뇨병 환자를 위한 지속적인 혈당 관찰 시스템 (CGMS, continuous glucose monitoring systems)에 대한 관심이 증가하고 있으며, 역오팔 광결정 수화젤 (IOPG, inverse opal photonic gels)이 가역적인 반사색의 변화에 의해 혈당 수준을 관찰할 수 있기 때문에 IOPG가 CGMS의 핵심기술로 전망 받고 있다. 본 연구에서는 4차 아민에 의한 phenylboronate의 분자 내 전하 안정화를 통해 생리적 pH에서도 혈당을 관찰할 수 있는 IOPG를 제작하였고, 보다 좋은 감응도를 위해 불소가 달린 PBA를 이용하여 수화젤을 제작하였다. 역오팔 광결정 수화젤의 주 형성 단량체인 2-Hydroxyethyl methacrylate (HEMA), 두 개의 서로 다른 전하 안정제인 (3-Acrylamidopropyl)trimethylammonium chloride (APTA)와 2-(methacryloyloxy)ethyltrimethylammonium chloride (MOETA)를 glucose와 boronate의 가역적인 결합 반응으로 혈당을 감지할 수 있는 3-acrylamidophenylboronic acid (3-AAPBA)와 공중합 하였다. 또한 감응성 개선을 목적으로 acrylamide (AAm) 와 3-fluoro-4-acrylamido- phenylboronic acid (3F4AAPBA)를 공중합 하였다. 수화젤은 ‘Directed Enhanced Evaporative colloidal assembly’ (DEECA) 방법으로 제작하였다. APTA와 MOETA를 포함하여 제작한 역오팔 광결정 수화젤은 생리적 pH인 pH 7.4에서 감응도가 증가 된 것을 확인하였고, 3F4AAPBA를 포함하는 수화젤에서 감응도가 더욱 개선되었고, 생리적 혈당 수치에서 RGB (red, green, blue)의 반사색이 나타나게 제작하였다.