인삼 사포닌의 생화학적 연구 (XVIII) : 쥐 간 균질물에 의한 인 지질 생합성에 미치는 인삼 사포닌의 영향 = Biochemical Study on Ginseng Saponin (XVIII) : The Effect of Ginseng Saponin on Phospholipid Biosynthesis in vitro

국문 초록 (Abstract)

쥐의 간 균질물을 효소원으로 사용하고 $(U-^{14}C)$-glycerol, $(U-^{14}C)$-sn-glycerol-3-phosphate 또는 $(1-^{14}C)$-palmityl CoA를 tracer로 사용하여, 인 지질 생합성에 미치는 인삼 saponin의 영향을 in vitro에서 관찰하여 다음과 같은 결과를 얻었다. 1. $(U-^{14}C)$-Glycerol을 사용한 실험에서 phosphatidyl ethanolamine 생합성은 인삼 saponin의 농도가 $10^{-4}{\sim}10^{-3}%$일 때 대조군 보다 10배 이상 증가되었다. 2. $(U-^{14}C)$-sn-Glycerol-3-phosphate를 사용한 실험에서 인삼 saponin의 농도가 $10^{-4}{\sim}10^{-3}%$ 일 때 대조군에 비해서 전 지질의 생합성이 촉진되었고, 인삼 saponin의 농도가 $10^{-4}%$ 일 때 phosphatidyl ethanolamine, phosphatidyl oholine 및 neutral lipid의 생합성은 대조군 보다 증가되었다. 3. $(1-^{14}C)$-Palmityl CoA를 사용한 실험에서 인삼 saponin의 농도가 $10^{-4}{\sim}10^{-3}%$ 일때 phosphatidyl ethanolamine 및 phosphatidyl choline의 생합성이 촉진되었다.

다국어 초록 (Multilingual Abstract)

It has been attempted in this study to observe the effect of ginseng saponin on phospholipid biosynthesis by rat liver homogenate using $(U-^{14}C)$-glycerol, $(U-^{14}C)$-sn-glycerol-3-phosphate, and $(1-^{14}C)$palmityl CoA as tracer in vitro and the following results were obtained. 1. Biosynthesis of phosphatidyl etanolamine using $(U-^{14}C)$-glycerol as tracer was increased as much as 10 times when the concentration of ginseng saponin in the reaction mixture was $10^{-4}\sim10^{-3}%$. 2. In case of experiment using $(U-^{14}C)$-sn-glycerol-3-phosphate as tracer, biosoynthesis of total lipids was also stimulated at concentration of $10^{-4}\sim10^{-3}%$ of ginseng saponin. Furthermore, biosynthesis of phosphatidyl ethanolamine and phophatidyl choline was increased in the reaction mixture containing $10^{-4}%$ ginseng saponin. 3. Using liver homogenate and $(1-^{14}C)$-palmityl CoA only, it was observed that the ginseng saponin $(10^{-4}\sim10^{-3}%)$ stimulated the biosynthesis of phosphatidyl choline and phosphatidyl ethanolamine. From the above experimental results, it seemed that adequate amounts of ginseng saponin might stimulate the biosynthesis of phospholipids significantly.