Scoparone from Artemisia capillaris Inhibits the Release of Inflammatory Mediators in RAW 264.7 Cells upon Stimulation Cells by Interferon-γ Plus LPS

다국어 초록 (Multilingual Abstract)

Scoparone is a major component of the shoot of Artemisia capillaris (Compositae), which has been used for the treatment of hepatitis and biliary tract infection in oriental countries. In the present study we observed that, scorparone exhibited no cytotoxic effect in unstimulated macrophages, but reduced the release of nitric oxide (NO) and prostaglandin E2 (PGE2) upon stimulation by IFN-γ/LPS or LPS. The inhibitory effects were found to be in conjuction with the suppression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in IFN-γ/LPS stimulated RAW 264.7 cells. Moreover, scoparone also attenuated the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6 in LPS-stimulated RAW264.7 cells. These results suggest that scoparone decreases the production of the inflammatory mediators such as NO and PGE2 in macrophages by inhibiting iNOS and COX-2 expression.

참고문헌 (Reference)

1 "a pivotaltranscription factor in chronic inflammatory diseases. N. Engl.J. Med." 336 : 1066-1071, 1997

2 "Treating patients with severesepsis. N. Engl. J. Med." 340 : 207-214, 1999

3 "TNF in the inflammatoryresponse. Chem. Immunol." 74 : 141-161, 2000

4 "Selectiveexpression of mitogen-inducible cycloocygenase inmacrophages stimulated with lipopolysaccharide. J. Biol.Chem." 25934-25938, 1992

5 "Screening forbiological activity and chemical composition of Euodiaborbonica var. borbonica" 64 : 283-288, 1999

6 "Role oftranscription factor NF-kB/Rel in induction of nitric oxidesynthase. J. Biol. Chem." 4705-4708, 1994

7 "Role ofcytokines in rheumatoid arthritis. Annu. Rev. immunol." 14 : 397-440, 1999

8 "Resistance toendotoxin shock in transgenic mice overexpressingendothelial nitric oxide synthase. Circulation. 100" 100 : 931-937, 2000

9 "Protectionby nitric oxide against liver inflammatory injury in animalscarrying a nitric oxide synthase-2 transgene. FASEB. J." 15 : 583-585, 2001

10 "Prostaglandins and nitric oxide as moleculartargets for anti-inflammatory therapy. Fitoterapia." 71 : -57, 2000

11 "Possiblemechanism of immunosuppressive effect of scoparone" 7 143-148, 1992a

12 "Molecular biologicalanalysis of nitric oxide synthases. Methods in Nitric OxideRsearch" 161-175, 1995

13 "Inhibition of NF-kB" 1448 : 77-84, 1998

14 "Inducible nitricoxide synthase and proinflammatory cytokine expression byhuman keratinocytes during acute urticaria. Mol. Med." 3 : 686-694, 1997

15 "Endotoxin-stimulatedproduction of rat hypothalamic interleukin-1beta in vivo andin vitro measured by specific immunoradiometric assay. J.Mol. Endocrinol." 31-36, 1993

16 "Differentialregulation of prostaglandin E2 and thromboxane A2production in human monocytes implications for the use ofcyclooxygenase inhibitors. J. Immuunol." 165 : 1605-1611, 2000

17 "Cytokines as endogenous pyrogens. J. Infect.Dis." 179 : -304, 1999

18 "A bacteriainducedswitch of sympathetic effector mechanismsarguments local inhibition of TNF-alpha and IL-6 secretion inthe spleen. FASEB. J." 14 : 1380-1388, 2000