Plant Regeneration from Hypocotyls of common buckwheat, (Fagopyrum esculentum Moench) and annual wild buckwheat, (F. homotropicum Ohnishi) = 보통 메밀 (Fagopyrum esculentum Moench)과 일년생 야생 메밀 (F. Homotropicum Ohnishi)의 胚軸培養 에 의한 植物體 再分化

다국어 초록 (Multilingual Abstract)

Plant regeneration from cultured hypocotyls may provide means for overcoming breeding barriers caused by heterostylic cross-incompatibility in interspecific crosses of common buckwheat(Fagopyrum esculentum Moench). Hypocotyles were excised from seed embryos of common buckwheat(cv. Miyazakizairai) and wild annual buckwheat(F. homotropicum Ohnishi) and cultured on MS medium supplemented with 0∼2.0 mg/l IAA and 0~2.0 mg/l BAP. Shoot was induced only on the medium containing 1.0 mg/l NAA and 1.0 mg/l BAP, shoot induction was promoted by 2.4-D and the frequency was lower in wild annual buckwheat than in common buckwheat. Roots were developed on White's medium containing 1.0 mg/l IBA. Whole plantlets were obtained following the production of adventitious roots from the shoots. When transferred from sterile culture condition to peat and soil medium, most plantlets were able to survive and grew to have flowers.
Key words : Common buckwheat(Fagopyrum esculentum), F. homotropicum Ohnishi, hypocotyle explants, tissue culture, plant regeneration

목차 (Table of Contents)

• Introduction
• Materials and Methods
• Results and Discussion
• 摘 要
• Literature Cited