식물 세포배양 및 융합을 통한 유용물질 개발-현탁 배양세포의 물질분석 = DEVELOPMENT OF USEFUL PRODUCTS THROUGH PLANT CELL FUSION AND CULTURE ANALYSIS OF USEFUL SECONDARY PRODUCTS IN CRLL SUSPENSION CULTURE

다국어 초록 (Multilingual Abstract)

Cell suspension cultures for Salix koreansis was well established at the supplements of 2,4-D with cytokinin at 10: 1 ratio, particulary the combination of 1.0 ㎎/ℓ 2,4-D with 0.1 ㎎/ℓ of zeatin. These combined rates of phytohormones are also effective to callus induction from S. koreansis leaf and its multiplication. Cultured media exhibited the great inhibitory effect on the germination of rice, barnyard grass and lettuce seeds, indicating the presence of biologically active substances in media. Several phenolic compounds such as pyrogallol, sinapic acid, cinamic acid, tannic + gallic and p-chlorobenzoic acid were detected in the cell suspension culture. The inhibitory effect exhibited by cultured media may be partly attributed to these phenolic compounds.