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茵蔯蒿가 흰쥐 肝조직내 Na^+·K^+ -ATPase 活性에 미치는 영향
金柱賢,申國鉉,趙台淳 成均館大學校 科學技術硏究所 1987 論文集 Vol.38 No.2
In order to investigate the effects of Artemisia on bile flow, the bile volume and the activities of Na^+·K^+-ATPase in liver plasma membrane and liver homogenate were examined in rats orally administered methanol extract of Artemisiae messer-schmidtiana Besser var. viridis Besser for 3 days. In the case of bile juice, the flow rate was increased 30∼70%. The activities of Na^+·K^+-ATPase was increased to 2.5 fold in liver homogenate and 2.1 fold in liver plasma membrane. On the other hand activities of Mg^++-ATPase was slightly increased in both liver homogenate and liver plasma membrane.
Seong, Jin Hyo,Kim, Dong Kie,Shin, Kwang Yong,Kim, Byung Ock,Kim, Myong Soo,Kim, Mi Kwang,Kook, Joong Ki 대한구강보건학회 2002 大韓口腔保健學會誌 Vol.26 No.4
본 연구는 치주질환과 관련된 병원성 세균 중 그람 음성 협기성 세균 종인 Prevorella intermedia (P. intermedia)와 Prevotella nigrescens (P. nigrescens)를 동시에 동정 및 검출할 수 있는 중합효소연쇄반응 프라이머를 개발하기 위하여 시행하였다. 모든 세균 종에 있어서 165 라이보송 RNA의 염기서 열에는 잘 보존된 부위와 종에 따라 다양한 부위가 있다. 이러한 특성을 이용하여 P. nigrescens와 P. intermedia의 알려진 165라이보솜 RNA의 염기서 열을 분석하여 두 세균 종에 공동으로 존재하는 하나의 프라이머와 각각의 세균 종에 특이하게 존재하는 다른 프라이머를 설계 및 제작하였으며, 이때 설계된 프라이머의 이름을 각각 Pin-F, Pi-R 및 Pn-R이라고 명명하였다. 이들의 종 특이성을 조사하기 위해 두 균주의 P. intermedia와 P. nigrescens지놈 DNA를 추출하여, 종 특이성을 중합효소연쇄반응법으로 관찰하였다. 이때 구강 내 6가지 다른 세균 종에서 같은 방법으로 중합효소연쇄반응을 실시한 결과 P. nigrescens와 P. intermedia 두 종만을 특이하게 검출함을 알 수 있었다. 또한 민감도를 조사하기 위해 두 세균 종에서 추출한 지놈 DNA를 4 ng에서 4 fg까지 희석하여 중합효소연쇄반응을 실시한 결과 400 fg의 지놈 DNA까지도 검출할 수 있었다. 이러한 결과를 종합할 때 이들 Pin-F, Pi-R 및 Pn-R프라이머 쌍은 P. inermedia와 P. nigrescens를 동시에 종특이적이며, 민감도 높게 검출 및 동정할 수 있을 것으로 사료된다.
Kim, Sungchul,Lee, Sanghyun,Shin, Jinwook,Kim, Youngkyun,Evnouchidou, Irini,Kim, Donghyun,Kim, Young-Kook,Kim, Young-Eui,Ahn, Jin-Hyun,Riddell, Stanley R,Stratikos, Efstratios,Kim, V Narry,Ahn, Kwangs Nature Publishing Group, a division of Macmillan P 2011 NATURE IMMUNOLOGY Vol.12 No.10
Major histocompatibility complex (MHC) class I molecules present peptides on the cell surface to CD8<SUP>+</SUP> T cells, which is critical for the killing of virus-infected or transformed cells. Precursors of MHC class I??presented peptides are trimmed to mature epitopes by the aminopeptidase ERAP1. The US2??US11 genomic region of human cytomegalovirus (HCMV) is dispensable for viral replication and encodes three microRNAs (miRNAs). We show here that HCMV miR-US4-1 specifically downregulated ERAP1 expression during viral infection. Accordingly, the trimming of HCMV-derived peptides was inhibited, which led to less susceptibility of infected cells to HCMV-specific cytotoxic T lymphocytes (CTLs). Our findings identify a previously unknown viral miRNA??based CTL-evasion mechanism that targets a key step in the MHC class I antigen-processing pathway.
Kim Hwa-Sook,Song Soo Keun,Yoo So Young,Jin Dong Chun,Shin Hwan Seon,Lim Chae Kwang,Kim Myong Soo,Kim Jin-Soo,Choe Son-Jin,Kook Joong-Ki The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.4
The objective of this study was to assess the strain-specificity of a DNA probe, Fu12, for Fusobacterium nucleatum subsp. nucleatum ATCC $25586^T$ (F. nucleatum ATCC $25586^T$), and to develop sets of strain-specific polymerase chain reaction (PCR) primers. Strain-specificity was tested against 16 strains of F. nucleatum and 3 strains of distinct Fusobacterium species. Southern blot hybridization revealed that the Fu12 reacted exclusively with the HindIII-digested genomic DNA of F. nucleatum ATCC $25586^T$. The results of PCR revealed that three pairs of PCR primers, based on the nucleotide sequence of Fu12, generated the strain-specific amplicons from F. nucleatum ATCC $25586^T$. These results suggest that the DNA probe Fu12 and the three pairs of PCR primers could be useful in the identification of F. nucleatum ATCC $25586^T$, especially with regard to the determination of the authenticity of the strain.
Kim, Chun Sung,Moon, In-Sung,Park, Ju-Hyun,Shin, Woo-Cheol,Chun, Hong Sung,Lee, Sook-Young,Kook, Joong-Ki,Kim, Heung-Joong,Park, Joo-Cheol,Endou, Hitoshi,Kanai, Yoshikatsu,Lee, Byung-Kwon,Kim, Do Kyun Pharmaceutical Society of Japan 2010 BIOLOGICAL & PHARMACEUTICAL BULLETIN Vol.33 No.7
<P>The purpose of this study was to examine the effect of 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH), an inhibitor of L-type amino acid transporters, on the cell growth suppression in KB human oral cancer cells and to study the roles of cell cycle regulatory factors in the BCH-induced growth inhibition. The effect of BCH on cell growth suppression and the influence of BCH to cell cycle regulatory factors in KB cell growth inhibition were examined using cell cycle analysis, immunoblotting and immunoprecipitation. The BCH treatment induced cell cycle arrest at G1 phase in KB cells. The expression of cyclin D3 was remarkably decreased by BCH treatment. The BCH inhibited the expression of cyclin-dependent protein kinase 6 (CDK6) in a time-dependent manner. In addition, the expression of CDK inhibitor p27 was increased by BCH treatment in KB cells, but not CDK inhibitors p21 and p15. These results suggest that, in KB cells, the inhibition of LAT1 by BCH causes cell cycle arrest at G1 phase by inhibiting cyclin D3–CDK6 complex whereas increasing expression of a CDK inhibitor p27.</P>
Kim, Hong,Jee, Young Mee,Song, Byung-Cheol,Shin, Jung Woo,Yang, Soo Hyun,Mun, Ho-Suk,Kim, Hyun-Ju,Oh, Eun-Ju,Yoon, Jung-Hwan,Kim, Yoon-Jun,Lee, Hyo-Suk,Hwang, Eung-Soo,Cha, Chang-Yong,Kook, Yoon-Hoh,K S. Karger AG 2006 Intervirology Vol.50 No.1
<P><I>Objectives:</I> Although hepatitis B virus (HBV) is endemic to Korea, no large-scale survey of HBV genotypes and serotypes based on sequence analysis has been performed. <I>Methods:</I> In the present study, we genotyped and serotyped HBV strains from 209 patients in two Korean regions, Seoul (107 patients) and Jeju (102 patients), an island off the southeastern Korean coast. Analyses were conducted using the direct sequencing method targeting the partial surface (S) gene (541 bp). <I>Results:</I> Phylogenetic analysis showed that all HBV strains from the 209 patients belonged to genotype C2 (100%). Of the 209 patients, 193 (92.3%), 12 (5.7%) and 1 (0.5%) were found to have the <I>adr</I>, <I>adw</I> and <I>ayr</I> serotypes, respectively. The other three strains (1.5%) showed unique serotype and were not typeable by sequence analysis. No HBV strains characteristic of Jeju island were observed. <I>Conclusions:</I> The extraordinary predominance of genotype C2 in chronic Korean patients, which is known to be associated with more severe liver disease than genotype B, suggests that the clinical manifestations of Korean HBV chronic patients are likely to differ from those found in other Asian countries, especially in Japan and Taiwan, where genotypes B and C coexist.</P><P>Copyright © 2007 S. Karger AG, Basel</P>
Shin, Jae Kook,Cheon, Jae Hee,Kim, Eun Sook,Yoon, Jin Young,Lee, Jin Ha,Jeon, Soung Min,Bok, Hyun Jung,Park, Jae Jun,Moon, Chang Mo,Hong, Sung Pil,Lee, Yong Chan,Kim, Won Ho The Korean Academy of Medical Sciences 2010 JOURNAL OF KOREAN MEDICAL SCIENCE Vol.25 No.7
<P>The purpose of this study is to evaluate the predictive capability of anorectal physiologic tests for unfavorable outcomes prior to the initiation of biofeedback therapy in patients with dyssynergic defecation. We analyzed a total of 80 consecutive patients who received biofeedback therapy for chronic idiopathic functional constipation with dyssynergic defecation. After classifying the patients into two groups (responders and non-responders), univariate and multivariate analyses were performed to determine the predictors associated with the responsiveness to biofeedback therapy. Of the 80 patients, 63 (78.7%) responded to biofeedback therapy and 17 (21.3%) did not. On univariate analysis, the inability to evacuate an intrarectal balloon (<I>P</I>=0.028), higher rectal volume for first, urgent, and maximal sensation (<I>P</I>=0.023, <I>P</I>=0.008, <I>P</I>=0.007, respectively), and increased anorectal angle during squeeze (<I>P</I>=0.020) were associated with poor outcomes. On multivariate analysis, the inability to evacuate an intrarectal balloon (<I>P</I>=0.018) and increased anorectal angle during squeeze (<I>P</I>=0.029) were both found to be independently associated with a lack of response to biofeedback therapy. Our data show that the two anorectal physiologic test factors are associated with poor response to biofeedback therapy for patients with dyssynergic defecation. These findings may assist physicians in predicting the responsiveness to therapy for this patient population.</P>