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YOO, SEUNG HEE,YOON, YOUNG GEOL,LEE, JEE SUK,SONG, YEON SUK,OH, JOON SEOK,PARK, BONG SOO,KWON, TAEG KYU,PARK, CHEOL,CHOI, YUNG HYUN,YOO, YOUNG HYUN Spandidos Publications 2012 International journal of oncology Vol.41 No.4
<P>The Bcl-2 protein is known to exert not only anti-apoptotic but also anti-autophagic activities. Numerous studies have demonstrated that etoposide, which is one of the most widely used cancer chemotherapy agents, induces apoptotic cell death. However, the exact molecular mechanism leading to cell death by etoposide remains to be resolved. This study aimed to dissect the mode of cell death induced by etoposide in Hep3B hepatoma cells. Furthermore, this study was conducted to examine whether etoposide overcomes the resistance conferred by Bcl-2 in Hep3B hepatoma cells. We observed that Hep3B cells treated with etoposide show not only apoptotic but autophagic phenotypes. Autophagy inhibition by 3-methyladenine (3MA) and caspase inhibition by zVAD-fmk effectively decreased autophagic and apoptotic phenotypes, respectively. However, either zVAD-fmk or 3MA only partially prevented cell death. These data indicate that etoposide concomitantly induces autophagic cell death and apoptosis in Hep3B cells. Importantly, etoposide can effectively induce cell death in Bcl-2-overexpressing Hep3B cells. Conversely, staurosporine, which exclusively induces apoptosis in Hep3B cells, did not efficiently induce cell death in Bcl?2?overexpressing Hep3B cells. Staurosporine-treated Hep3B cells also showed an autophagic phenotype. While autophagy is cell death-inducing in Hep3B cells treated with etoposide, it is cytoprotective in Hep3B cells treated with staurosporine. To this end, we observed that etoposide-induced mixed type of programmed cell death is associated with the dissociation of Bcl-2 from Beclin-1. Taken together, etoposide induces a mixed type of programmed cell death and overcomes the resistance conferred by Bcl-2 in Hep3B hepatoma cells.</P>
Biochemical Quantitation of PM2 Phage DNA as a Substrate for Endonuclease Assay
Joo, Yoo-Jin,Kim, Hee-Ju,Lee, Jae-Yung,Kim, Joon The Microbiological Society of Korea 2004 The journal of microbiology Vol.42 No.2
Bacteriophage PM2 has a closed circular form of double stranded DNA as a genome. This DNA from the phage is a useful source for nick-circle endonuclease assay in the fmol range. Due to difficulties in the maintenance of viral infectivity, storage conditions of the phage should be considered for the puri-fication of PM2 DNA. The proper condition for a short-term storage of less than 2 months is to keep the PM2 phage at 4$^{\circ}C$; whereas the proper condition for a long-term storage of the PM2 phage for over 2 months is to keep it under liquid nitrogen in 7.5 % glycerol. The optimal conditions for a high yield of phage progeny were also considered with the goal to achieve a successful PM2 DNA preparation. A MOI(Multiplicity Of Infection) of 0.03, in which the OD$\sub$600/ of the host bacteria was between 0.3 and 0.5, turned out to be optimal for the mass production of PM2 phage with a burst size of about 214. Considerations of PM2 genome size, and the concentrations and radiospecific activities of purified PM2 DNA, are required to measure the endonuclease activity in the fmol range. This study reports the proper quantitation of radioactivity and the yield of purified DNA based on these conditions.
Application of Non-photochemical Quenching on Screening of Osmotic Tolerance in Soybean Plants
Sei Joon Park(박세준),Hyun Hee Kim(김현희),Tae Seok Ko(고태석),Myong Yong Shim(심명룡),Sung Yung Yoo(유성녕),So Hyun Park(박소현),Tae Gyeong Kim(김태경),Ki Cheol Eom(엄기철),Sun Hee Hong(홍선희),Tae Wan Kim(김태완) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.3
한발과 염 스트레스에 대한 콩의 저항성 검정에 있어서 Non-photochemical quenching (NPQ)을 적용하기 위하여, 저항성 콩 (신팔달콩 2호)와 대조구 콩 (태광콩)을 이용하여 제한적 관수 (50 m/pot/day) 와 염 (200 mmol NaCl) 처리를 한 후, 엽록소 형광반응의 변수, maximum efficiencies of photosystem II photochemistry (Fv/Fm), efficiencies of photosystem II photochemistry (ΦPSII), NPQ와 CO₂ 동화율 (PN) 을 측정하였다. 콩 두 품종의 엽 수분포텐셜은 한발처리에서 -0.2 MPa에서 -0.8 MPa로, 염처리에서는 -0.7 MPa에서 -1.7 MPa로 감소하였다. 염처리에서 엽 수분함량의 감소는 신팔달콩 2호에서 적었다. 두 품종 모두 엽의 수분포텐셜이 감소함에 따라 Fv/Fm 은 변화가 없었으며, ΦPSII 와 PN 는 감소하였다. NPQ의 경우, 신팔달콩 2호은 한발과 염처리에 모두에서 반응이 나타난 반면, 태광콩에서는 한발처리에서만 나타났다. 두 품종의 모든 처리에서 ΦPSII 와 PN간에 정의 상관 관계를 보였으나, PN 의 감소에 대한 ΦPSII 의 감소 정도가 신팔달콩 2호에서 적었다. 또한 삼투처리에 따른 ΦPSII 의 감소와 NPQ의 증가는 신팔달콩 2호에서만 나타나 ΦPSII 와 NPQ 간의 부의 관계가 유지된 반면, 태광콩에서는 염처리에서 이들간의 연관성이 없었다. 따라서 본 연구는 삼투 저항성의 검정에 있어서 엽록소 형광반응의 단일변수(Fv/Fm, ΦPSII, 및 NPQ)의 이용보다 ΦPSII 와 NPQ 의 상호관계 분석이 더 유효한 것을 제시하였다. Non-photochemical quenching (NPQ) values for utilizing them to detect osmotic tolerance in plants were examined with two different soybean cultivars, an osmotic tolerant soybean (Shinpaldalkong 2) and a control soybean (Taekwangkong). Two different stresses were applied to the cultivars as the restricted irrigations of 200 and 50 ml water pot<SUP>-1</SUP> d<SUP>-1</SUP> for 5 days for a control and a drought stress, respectively, and a sodium chloride solution of 200 mmol for 6 days for a salt stress. The intact leaves of the two cultivars after treatment were used to measure chlorophyll fluorescence parameters, maximum efficiencies of photosystem II photochemistry (Fv/Fm), efficiencies of photosystem II photochemistry (ΦPSII), CO₂ assimilation rate (PN), and NPQ. Leaf water potentials of the two cultivars decreased from ? 0.2 to ? 0.8MPa by a drought treatment and from ? 0.7 to ? 1.7MPa by a salt treatment. Leaf water content of Shinpaldalkong 2 after a salt treatment was less decreased than that of Taekwangkong. Fv/Fm values of both cultivars were not changed, while ΦPSII and PN were decreased proportionally to leaf water potential decrease. The response of NPQ was occurred in Shinpaldalkong 2 under the drought and salt stresses. With Taekwangkong cultivar, only drought stress referred NPQ response. The cultivar differences on chlorophyll fluorescence parameters were found in the relationships between ΦPSII and PN, and between NPQ and ΦPSII. Although the positive relationships between ΦPSII and PN were established on all treatments of both cultivars, the decreasing rate of ΦPSII to PN was smaller in Shinpaldalkong 2 than Taekwangkong. The NPQ was increased according to the decrease of ΦPSII by osmotic treatments in Shinpaldalkong 2. The complementary relationships between NPQ and ΦPSII were well maintained at all treatments in Shinpaldalkong 2, while these relationships were lost at a salt treatment in Taekwangkong. Taken together, the results suggest that analysis of complementary relationships between ΦPSII and NPQ could be more valuable and applicable for determining osmotic tolerance than single analysis of each parameter such as Fv/Fm, ΦPSII and NPQ.
Analysis of Chlorophyll Fluorescence in Transgenic Soybean under Drought and Salt stress
Sei Joon Park,Ji Young Kim,Hyun Hee Kim,Sung Yung Yoo,So Hyun Park,Sun Hee Hong,Tae Seok Ko,Tae Wan Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Development of transgenic plant with desirable traits to cultivated plant is one of the important procedures in plant molecular breeding. However, applicable assessment of transgenic plant in laboratorial scale is not much except cultivating transgenic plant for a whole life in field condition. Here, we analyzed chlorophyll fluorescence in three transgenic soybean lines with AtMYB44 transcription factor for assessment of photosynthetic activity under abiotic stresses such as drought. Soybean varieties used in this study were ‘Bert’ and ‘Bert’ derived three transgenic soybeans, ‘AtMYB44 CM35101’, ‘AtMYB44 CM2471’, and ‘AtMYB44 CM4481’. Analyzed five different chlorophyll fluorescence variables are maximum PSII quantum yield (QY_max), steady state PSII quantum yield (QY_Lss), steady state non-photochemical quenching (NPQ_Lss), coefficient of photochemical quenching in steady-state (Qp_Lss), and fluorescence declineratio in steady-state (Rfd_Lss). To determine main chlorophyll fluorescence variable affected by abiotic stress, principal component analysis (PCA) was conducted with five chlorophyll fluorescence variables measured from four varieties. QY_Lss and NPQ_Lss were main chlorophyll fluorescence variables to evaluate abiotic stress, particularly in drought stress. In comparison with transgenic soybean lines based on chlorophyll fluorescence variables, ‘AtMYB44 CM2471’ and ‘AtMYB44 CM4481’ are more tolerant to drought than the others. Interestingly, three transgenic soybean lines which have a same AtMYB44 gene with different regions of chromosome revealed the quite different responses of chlorophyll fluorescence to drought treatment.
박준옥,이군순,주인고,최승준,김종민,한설혜,김양수,김태균,정재용,박유환 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.2
Eosinophilic gastroenteritis is an uncommon disease characterized by peripheral eosinophilia, eosinophilic infiltration of various areas of the gastrointestinal tract with gastrointestinal symptoms such as abdominal pain, vomiting, diarrhea and rarely ascites. This disorder was pathologically classified into three major types: predominant mucosal, predominant muscle layer and predominant subserosal. Although its etiology and pathogenesis was not yet definitely established, its treatment was steroid administration and the overall prognosis was favorable. We report a case of eosinophilic gastroenteritis with features of the predominant subserosal type, presented as an ascites and ileus.
Sei Joon Park(박세준),Do Yun Kim(김도연),Sung Yung Yoo(유성녕),Hyun Hee Kim(김현희),Tae Seok Ko(고태석),Myong Yong Shim(심명룡),So Hyun Park(박소현),Ji A Yang(양지아),Ki Cheol Eom(엄기철),Sun Hee Hong(홍선희),Tae Wan Kim(김태완) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.3
서리태콩 (Glycine max Merr. var Seoritae)에서 광원에 따른 엽의 광합성 변화를 구명하기 위하여 콩의 제 1복엽이 완전 전개되었을 때 3일 동안 빛을 차단한 후 UV-B 와 일반광에 노출시켜 색소 함량과 엽록소 형광반응의 변화를 측정하였다. 암처리에서 엽록소 함량은 감소하고, 일반 광에서 회복하였다. 카로티노이드와 안토시아닌 함량은 UV-B 조사한 처리구에서 증가하였다. 엽록소 형광분석을 이용한 광합성 능률을 분석한 결과, 암처리가 진행 됨에 따라 Fv/Fm, F"v/F"m, ΦPSII 및 NPQ는 감소하였다. 모든 변수들은 일반광에 노출되면서 회복하였으나 UV 처리한 것은 암처리 72시간의 수치와 큰 변화가 없었다. 이를 통하여 암처리 48시간 경과함으로 엽록체가 에티오플라스트로 전환되며, 일반광을 조사하였을 시 광합성 관련 광계가 복구되지만, UV-B의 강한 광이 조사되었을 때 광계가 회복되지 못하는 것으로 사료되었다. Etiolation of plant leaves evoke to be photosynthetically inactive because plant leaves are unable to convert photochlorophyllide to chlorophyllide in the absence of light. In addition, UV-B radiation plays an important role in photomorphogenesis and excessive UV-B radiation decreases photosynthesis and causes to damage to cellular DNA. In the present study, two electrical lights obtained with the ultraviolet lamp and moderate lamp were employed to young plants soybean (Glycine max Merr. var Seoritae). After treatment of different lights, young plants were harvested for the determination of pigment contents and chlorophyll fluorescence. The contents of carotenoids and anthocyanins were significantly enhanced with the excessive UV-B radiation. Excessive UV-B light reduced dramatically photosynthetic efficiency causing an irreversible damage on PSII in comparison to the controls treated under normal illumination. As the treatment of normal illumination after dark treatment, the contents of carotenoids and anthocyanains were not changed in the leaves and photosynthetic ability were retained. Therefore, Seoritae soybean leaves might protect themselves from excessive UV-B radiation with up-regulation of antioxidants.