Distribution of Bi–MnS Inclusions Existing in 1215MS Steels: Correlation with Thermal Physical Coefficient

Jian‑bo Xie,Bei‑bei Liu,Xiang‑yu Wu,Jian‑xun Fu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.5

This work aims to comprehensively summarize the types and distribution of the isolated Bi particles and MnS inclusions inthe Bi-bearing steels, to study the precipitated behavior of sulfide, and to study the effect of Bi particles on grain sizes andthe volume thermal expansion coefficients of the steels. Results show that the average percentage of the isolated Bi particles,semi-encapsulation, complete encapsulation, back-bridge, tiny encapsulation, cavity bridge Bi–MnS inclusions in the Bibearingsteels was 16.89%, 20.49%, 3.44%, 16.89%, 31.63%, 10.66%, respectively. The average equivalent diameters of thegrains in the 0 ppm, 130 ppm, 240 ppm, 760 ppm, 1200 ppm and 2300 ppm Bi content steels were 20.88, 20.17, 19.54, 18.13,13.98 and 13.25 μm, respectively. The precipitated equilibrium mass concentration product of the MnS (w[Mn]·w[S]) wascalculated to be 1.07, and the solid fraction fS value was 0.61. At 950 °C, the volume thermal expansion coefficient change(ΔVE) in 760 ppm Bi content steel was minimum 4 × 10–10%, while at 461.6 °C the ΔVE was maximum, 0.09%, which meansthat Bi affected the volume thermal expansion of the steel strongest in this temperature range.

Disruption of ecdysis in Leptinotarsa decemlineata by knockdown of chitin deacetylase 1

Jian-Jian Wu,Li-Li Mu,Zhong-Chao Chen,Kai-Yun Fu,Wen-Chao Guo,Chao Li,Guo-Qing Li 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2

Chitin deacetylases (CDAs) catalyze N-deacetylation of chitin, a crucial process for chitin modification. In the present paper, LdCDA1 was identified in Leptinotarsa decemlineata. It was copiously expressed in larval foregut, hindgut and epidermis. Just before the molt in the first, second and third larval instars, the mRNA levels of LdCDA1 were high. In the fourth (final)-instar larvae, a peak occurred 4 days after ecdysis. In vivo results revealed that LdCDA1 transcriptionally responded, positively and negatively respectively, to 20-hydroxyecdysone and juvenile hormone titers. Moreover, knockdown of LdCDA1 significantly reduced foliage consumption, lengthened developing period and prevented growth in the final instar larvae. Three distinct lethal phenotypes were noted in the LdCDA1 RNAi larvae. About 30% of the RNAi larvae became moribund and finally died; approximately 50% of deformed pupae died as pharate adults; and around 20% of LdCDA1 depleted pupae finally emerged as abnormal adults and eventually died within 1 week after emergence. Furthermore, chitin content was low and the mRNA levels of five chitin biosynthesis transcripts (LdUAP1, LdUAP2, LdChSAa, LdChSAb and LdChSB) were significantly declined in the LdCDA1 RNAi larvae. In addition, glucose, trehalose and glycogen contents were increased in the LdCDA1 depleted hypomorphs, along with highly expressed genes coding for trehalose and glycogen synthesis enzymes. The findings provide a compelling piece of evidence that CDA1 is critical for chitin deposition in L. decemlineata. Moreover, LdCDA1 may be a potential target for control of the larvae.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Dual-pumped nondegenerate four-wave mixing in semiconductor laser with a built-in external cavity

Wu, Jian-Wei,Qiu, Qi,Hyub Won, Yong IOP 2017 Journal of optics Vol.19 No.4

<P>In this paper, a semiconductor laser system consisting of a conventional multimode Fabry–Pérot laser diode with a built-in external cavity is presented and demonstrated. More than two resonance modes, whose peak levels are significantly higher than other residual modes, are simultaneously supported and output by adjusting the bias current and operating temperature of the active region. Based on this device, dual-pumped nondegenerate four-wave mixing—in which two pump waves and a single signal wave are simultaneously fed into the laser, and the injection power and wavelength of the injected pump and signal waves are changed—is observed and discussed thoroughly. The results show that while the wavelengths of pump wave A and signal wave S are kept constant, the other pump wave B jumps from about 1535 nm to 1578 nm, generating conversion signals with changed wavelengths. The achieved conversion bandwidth between the primary signal and the converted signal waves is broadly tunable in the range of several terahertz frequencies. Both the conversion efficiency and optical signal-to-noise ratio of the newly generated conversion signals are adopted to evaluate the performance of the proposed four-wave mixing process, and are strongly dependent on the wavelength and power of the injected waves. Here, the attained maximum conversion efficiency and optical signal-to-noise ratio are close to −22 dB and 15 dB, respectively.</P>

MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3

Wu, Jian-Hong,Yao, Yong-Liang,Gu, Tao,Wang, Ze-You,Pu, Xiong-Yong,Sun, Wang-Wei,Zhang, Xian,Jiang, Yi-Biao,Wang, Jian-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

Protein-protein Interaction Network Analyses for Elucidating the Roles of LOXL2-delta72 in Esophageal Squamous Cell Carcinoma

Wu, Bing-Li,Zou, Hai-Ying,Lv, Guo-Qing,Du, Ze-Peng,Wu, Jian-Yi,Zhang, Pi-Xian,Xu, Li-Yan,Li, En-Min Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Lysyl oxidase-like 2 (LOXL2), a member of the lysyl oxidase (LOX) family, is a copper-dependent enzyme that catalyzes oxidative deamination of lysine residues on protein substrates. LOXL2 was found to be overexpressed in esophageal squamous cell carcinoma (ESCC) in our previous research. We later identified a LOXL2 splicing variant LOXL2-delta72 and we overexpressed LOXL2-delta72 and its wild type counterpart in ESCC cells following microarray analyses. First, the differentially expressed genes (DEGs) of LOXL2 and LOXL2-delta72 compared to empty plasmid were applied to generate protein-protein interaction (PPI) sub-networks. Comparison of these two sub-networks showed hundreds of different proteins. To reveal the potential specific roles of LOXL2- delta72 compared to its wild type, the DEGs of LOXL2-delta72 vs LOXL2 were also applied to construct a PPI sub-network which was annotated by Gene Ontology. The functional annotation map indicated the third PPI sub-network involved hundreds of GO terms, such as "cell cycle arrest", "G1/S transition of mitotic cell cycle", "interphase", "cell-matrix adhesion" and "cell-substrate adhesion", as well as significant "immunity" related terms, such as "innate immune response", "regulation of defense response" and "Toll signaling pathway". These results provide important clues for experimental identification of the specific biological roles and molecular mechanisms of LOXL2-delta72. This study also provided a work flow to test the different roles of a splicing variant with high-throughput data.

Protraction of mandibular molars through a severely atrophic edentulous space in a case of juvenile periodontitis

Jian-chao Wu,Yu-ting Zheng,Yi-jun Dai 대한치과교정학회 2020 대한치과교정학회지 Vol.50 No.2

Moving the mandibular posterior teeth into a severely atrophic edentulous space is a challenge. A carefully designed force-and-moment system that results in bodily protraction of the posterior teeth with balanced bone resorption and apposition is needed in such cases. This report describes the treatment of a 19-year-old woman with missing mandibular first molars due to juvenile periodontitis. Miniscrews were used as absolute anchorage during protraction of the mandibular second and third molars. Bodily mesial movement of the mandibular second and third molars was achieved over a distance of 11 to 17 mm after 39 months of orthodontic treatment. [Korean J Orthod 2020;50(2):145-154]

Incarcerated internal hernia within a huge irreducible parastomal hernia with intestinal obstruction

Jian-Ying Lou,Yu-lian Wu,Dan Wu 대한외과학회 2012 Annals of Surgical Treatment and Research(ASRT) Vol.83 No.3

We report an incarcerated internal hernia in a huge irreducible parastomal hernia-“hernia within hernia.” A 70-year-old obese woman with diabetes who underwent an abdomino-perineal resection 20 years ago was admitted to our hospital with 20 years history of a huge irreducible bulge, 25 cm in diameter. An internal hernia due to an adhesive band extending from the sac wall to proximal colon was found in the parastomal hernia sac during an emergency laparotomy. We cut off the distal colon and relocated the colostomy stoma. The patient was discharged uneventfully 2 weeks after the surgery and was readmitted to have a further laparoscopic hernia repair 8 months later. Unfortunately, an unrecognized enterotomy occurred during the secondary surgery that led to an additional laparotomy during which the mesh was not contaminated by the bowel contents and was kept in place. At 22-month follow-up, there were no evidences of recurrence.

On Resolving Key Escrow Problem in HIBE and HIBS

Jian-Wu Zheng,Jing Zhao,Xin-Ping Guan 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.6

In traditional hierarchical identity based cryptosystems (HIBC), non-leaf entities as level PKGs are usually capable of deriving private keys for their descendants with use of their private keys, non-leaf entities can therefore act (decrypt or sign) on the behalf of their arbitrary descendants. This is called key escrow problem of HIBC. In order to resolve key escrow problem, a new technique – Identifier Discrimination is proposed in this paper for composing private keys for entities in hierarchy. With the technique, an identity selective secure HIBE scheme is constructed under Decisional Bilinear Diffie- Helleman (DBDH) assumption in standard security model, in which any identity is incapable of deriving private keys for any of its descendants with use of its private key, and the privilege of generating private keys for each individual descendant is delegated by the root PKG through authorization, that we call Authorization Delegation. Moreover, a new hierarchical identity based signature (HIBS) scheme is constructed from our HIBE construction, by applying Naor transformation of an identity-based signature (IBS) out of an IBE. Because of the inability of deriving its descendants’ private keys with use its private key, an entity therefore cannot sign messages on behalf of any of its descendants, thus guaranteeing that authenticity and non-repudiation properties are achieved in our HIBS system.

Current and power quality multi-objective control of virtual synchronous generators under unbalanced grid conditions

Wu, Jian,Liu, Tong,Qiu, Tianyi,Xu, Dianguo The Korean Institute of Power Electronics 2020 JOURNAL OF POWER ELECTRONICS Vol.20 No.2

In recent years, with the large-scale application of distributed power sources in the power grid, the power grid is moving toward low inertia and low damping. The virtual synchronous generator (VSG) has become a hot topic for scholars since it can simulate the moment of inertia, damping and frequency modulation of synchronous generators. Due to its unbalanced load distribution and the random variation of power loads, grid voltage is asymmetrical. Under grid voltage asymmetry, a VSG experiences output current imbalance and power oscillation. The grid current imbalance further reduces the transformer's three-phase voltage, which makes it unequal, cyclic, and very easy to cause electricity accidents. Aiming at this problem, this paper proposes three modes of current balance, reactive power balance and active power balance without changing the characteristics of VSGs. The output current balance and power are constant when the grid voltage is asymmetrical. The effectiveness and feasibility of the control strategy are verified by simulation and experimental results, which provides an effective scheme for balanced and stable operation of the grid.