국내산 및 수입산 참돔의 중금속 함량 및 항산화 활성과 세포독성 효과 비교

황성연(Seong Yeon Hwang),배진한(Jin Han Bae),임선영(Sun-Young Lim) 한국생명과학회 2015 생명과학회지 Vol.25 No.4

본 연구에서는 국내에서 주로 소비되는 국내산 및 수입산 참돔의 일반성분과 중금속 함량을 비교 분석하였고 생리활성을 비교하기 위하여 참돔을 용매의 극성에 따라 추출하여 항산화 및 세포독성 효과에 대해 연구하였다. 수입산과 비교했을 때 국내산 참돔은 높은 함량의 수분과 낮은 함량의 조지방, 조단백질 및 회분을 나타내었다. 국내산 및 수입산 참돔의 중금속 함량을 비교한 결과 원산지에 따른 유의적 차이를 보였으며 국내산 참돔의 수은 함량은 0.08 mg/kg으로 수입산 참돔보다 높은 함량을 나타내었으나 납의 경우 원산지에 따른 유의적 차이는 보이지 않았다. 국내산 및 수입산 참돔의 A+M 및 MeOH 추출물을 인체 섬유육종세포(HT-1080)에 처리하였을 때, 세포 내 활성산소종 생성 저해효과를 살펴 본 실험 결과, 국내산 및 수입산 참돔 추출물들에 의한 활성산소종 생성 억제효과를 비교해 보면 수입산 참돔 추출물에 의한 저해효과가 높았으며 두 추출물들 중 MeOH 추출물에 의한 저해효과가 높았음을 알 수 있었다. 인체 암세포들(AGS 및 HT-29)들에 대한 세포독성 활성 결과에서 AGS 암세포의 경우 국내산 참돔 A+M 및 MeOH 추출물들에 의한 세포독성 효과가 수입산 참돔A+M 추출물들보다 높았다. HT-29 암세포에 대해서는 국내산 참돔A+M 추출물에 의한 세포독성 효과가 수입산 참돔 A+M 추출물에 의한 것보다 높았고 MeOH 추출물에 의한 세포독성 효과는 유사했다. 따라서 본 연구 결과를 기초자료로 하여 향후 참돔의 다양한 생리활성 규명이 필요하다고 사료된다. This study compared the heavy metal contents and the effects of extracts from domestic and imported red sea bream on the antioxidant activity and cytotoxicity of human cancer cell lines. The antioxidant activity was measured using the fluorescently sensitive dye, 2"-7" dichlorofluorescein-diacetate (DCFH-DA), and antiproliferative activity against AGS human gastric adenocarcinoma and HT-29 human colon cancer cell lines, which was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Domestic red sea bream had a higher mercury content when compared to imported red sea bream, but there was no significant difference in the lead content. Treatments with acetone/methylene chloride (A+M) and methanol (MeOH) extracts from domestic and imported red sea bream dose-dependently decreased the H2O2 induced ROS production, compared to the control. The cell viability showed that treatments with the A+M and MeOH extracts had cytotoxicity in the growth of AGS and T-29 cancer cells. In the case of AGS, the extracts from the domestic red sea bream were higher in inhibiting cancer cell growth, compared to imported red sea bream. Our results demonstrate that the heavy metal contents of domestic and imported red sea bream were below the limit of the Food Code of Korea. The results of the biological activities indicate that the antioxidant activity of extracts from imported red sea bream was more effective, while the extracts from the domestic red sea bream were stronger in cytotoxic activity.

Megalocytivirus 감염 해산 어류에서 나타나는 임상증상의 정량적 변화 분석

진지웅 ( Ji Woong Jin ),조혜진 ( Hye Jin Cho ),김광일 ( Kwang Il Kim ),정준범 ( Joon Bum Jeong ),박경현 ( Gyeong Hyun Park ),정현도 ( Hyun Do Jeong ) 한국어병학회 2011 한국어병학회지 Vol.24 No.2

In quantitative studies of clinical signs, rock bream of adults and juveniles infected with Megalocytivirus IVS-1 isolated from rock bream (Oplegnathus fasciatus) in Korea showed average 4.49±1.13 and 4.85±1.06 of spleen index respectively. In challenge experiments, Megalocytivirus IVS-1 induced 100% cumulative mortality in both adult and juvenile rock bream. However we found 60% cumulative mortality in juvenile red sea bream (Pagrus major) even after 30 days of injection, which contradicted with the results of other laboratories. Interestingly, IVS-1 infected red sea bream of the same juvenile size with rock bream showed lower spleen index compared to that of rock bream. In real-time PCR, there was continuous increasing of the numbers of viral copies (2.03×10(7) copies/mg) in the spleen of juvenile rock bream infected, which were different from those in adult rock bream showing plateau level after reaching to the peak level. Moreover, enlarged cell numbers in the infected spleen were also increased continuously in the juvenile but not in adult of rock bream, even decreased after reaching to peak level. Consequently, significant differences in clinical signs: cumulative mortality. spleen index and viral copy number were found between rock bream and red sea bream, but not between adult and juvenile rock bream. Certainly quantitative expression of clinical sign as in this study may be a way to compare the progression of megalocitiviral disease more accurately in different species or physiological conditions.

Amoxacillin의 경구투여에 따른 양식 어류(넙치, 조피볼락, 참돔)의 근육조직내 잔류량의 변화

정희식,김석,민원기,이후장,Chung, Hee-Sik,Kim, Suk,Min, Won-Gi,Lee, Hu-Jang 한국식품위생안전성학회 2006 한국식품위생안전성학회지 Vol.21 No.4

The residue depletion of amoxicillin was investigated in the olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major) after 7 days treatment with medicated feed at a dose of 400 mg/kg bw/day. Fishes were sampled for muscle on 1st, 2nd, 3rd, 4th, and 5th day after treatment. Amoxicillin concentrations were determined by high performance liquid chromatography with fluorescence detector. The recovery rates of amoxicillin in muscle samples ranged 84.3-101.3% and 75.0-91.5% for the concentration of 0.05 mg/kg and 0.1 mg/kg, respectively. Amoxicillin concentrations detected on 1st day after treatment were 0.137, 0.131, and 0.172 mg/kg in the muscle of olive flounder, rockfish, and red sea bream, respectively. After a withdrawal of 3 days, muscle concentrations were 0.012, 0.010, and 0.017 mg/kg in the olive flounder, rockfish, and red sea bream, respectively. Amoxicillin was not detectable in muscle samples on 4 days following withdrawal of the medicated feed. From results of the present study, a withdrawal period of amoxicillin is proposed on 4 days after 7 days treatment with medicated feed at a dose of 400 mg/kg bw/day to avoid the presence of excessive residues of the edible muscles of olive flounder, rockfish, and red sea bream.

Light-Dark and Food Restriction Cycles in Red sea bream, Pagrus major: Effect of Zeitgebers on Demand-feeding Rhythms

Choe Yong-Gwon,Choi Jae-Eun,Roh Duk-Whan,Bae Bong-Seong,Choi Cheol-Young The Korean Society of Fisheries and Aquatic Scienc 2001 Fisheries and Aquatic Sciences Vol.4 No.3

Red sea bream, Pagrus major a fish species characterized by its dualistic feeding pattern, was investigated to study the synchronizing effect of light and food on the demand-feeding rhythm. Nocturnal and diurnal red sea bream, both in groups and individually, were exposed to restricted-feeding and Light-Dark cycles of different periods. The phase relationship between both zeitgebers was also studied. In some cases, food availability restricted to the light or dark phase contrary to that of the previous feeding phase changed a diurnal feeding pattern into nocturnal and vice versa, suggesting that food can be one of the switching factors that decides whether the circadian system of red sea bream is diurnal or nocturnal. However, the fact that the feeding pattern of some fish was unrelated with the phase in which food was available suggests that other internal and/or external-factors could be involved in the temporal flexibility of red sea bream.

도미와 유사어종의 식품학적 특성비교 분석에 관한 연구

정지용(Ji-Yong Chung),정영미(Young Mi Jung) 한국조리학회 2017 한국조리학회지 Vol.23 No.2

본 연구는 도미회의 원료판별 및 도미유사어종간의 품질을 비교하였다. 도미 및 도미유사어종의 일반 성분을 측정한 결과는 다음과 같다. 근육 수분 함량, 단백질 함량 및 지방 함량 K, P, C는 도미의 근육에서 비교적 높은 값을 보였다. Fe는 함량이 낮았다. 중금속 성분을 측정한 결과, 도미와 숭어에서는 Cd가 검출되지 않았다. 붉은 광산에서 0.01±0.00 mg/kg이 검출되었다. 다른 중금속은 기준치 이하이거나 검출되지 않았습니다. 전기영동 결과, 붉은 띠무늬의 66∼55 kDa에서 나타난다. 도미와 가숭어의 경우, 밴드의 독특한 특징은 보이지 않았습니다. 도미의 경우 유사한 어류와 도미 물고기 사이에 식품 과학에 큰 차이가 없으나, 도미회가 비싼 가격으로 유통되고 있다. 연구 결과를 바탕으로 소비자의 안전한 음식 섭취와 올바른 유통문화 확산이 이뤄지길 바란다. 식품성분 분석 자료의 공유와 구별법을 교육하여 소비자가 원하는 가격으로 식재료를 구입할 수 있는 환경이 조성되어지길 바란다. 나아가 도미 외 타어종의 식품판별과 식품학적 연구가 연구되어지길 바란다. This study was conducted to compare the food quality of domesticated species. Consumers surveyed for safe food intake and proper culture of food distribution. The results of the comparison study are as follows. Muscle moisture content, protein content, and fat content. K, P, and C showed relatively high values in the muscle of the sea bream. Fe showed low contents. As a result of measuring heavy metal component, Cd was not detected in sea bream and mullet, but 0.01±0.00 mg/kg was detected in red mine. Other heavy metals were below the reference value or were not detected. Electrophoresis results showed that the band appeared at in red minefish. In the case of sea bream and swordfish, no distinctive features of the band were shown. In the case of sea bream, there was little difference in food science between the similar fish species and the red sea bream fish, but price was different. An environment should be created for consumers to buy the right ingredients at the price they want. It is necessary to educate consumers about food ingredients immediately.

Molecular Characterization of Interferon Regulatory Factor 10 in Red Sea Bream (Pagrus major) and Expression Analysis Following RSIV Infection

우원식,강경식,김경호,손하정,손민영,한현자,박찬일 한국수산해양교육학회 2023 수산해양교육연구 Vol.35 No.3

Interferon regulatory factors (IRFs) exhibit antiviral and antibacterial capabilities. In this research, we isolated the IRF10 genes from red sea bream (Pagrus major) and examined their expression following red sea bream iridovirus (RSIV) infection. Analysis of multiple sequences identified preserved components, such as the IRF-association domain, nuclear localization signal, and DNA-binding domain. Phylogenetic examination showed that PmIRF10 grouped with IRF10 equivalents in fish. We utilized quantitative real-time PCR to explore IRF10 expression in normal tissues, revealing increased expression levels in the gill and spleen. Our results indicate that PmIRF10 plays a role in the immune response, as evidenced by the controlled expression levels of IRFs after RSIV infection in red sea bream. This finding aligns with prior research on different fish species. Overall, this investigation characterizes IRF genes in red sea bream and provides a basis for comparative analysis of IRF genes.

Muscle Tissue Distribution Level after Dipping Administration of Neomycin in Olive Flounder (Paralichthys olivaceus), Rockfish (Sebastes schlegeli), and Red Sea Bream (Pagrus major)

Hee Sik Chung,Won Chul Jung,Dong Hyeok Kim,Ho Yeong Shon,Jeong Ju Lim,Gon Sup Kim,Suk Kim,Hu Jang Lee 한국실험동물학회 2007 Laboratory Animal Research Vol.23 No.4

After one day treatment with dipping water at a dose of neomycin 140 ㎎/ton water, the residue depletion of neomycin was investigated in cultured marine fishes olive flounder (Paralichthys olivaceus), rockfish (Sebastes schlegeli), and red sea bream (Pagrus major). Muscles of fishes were sampled on 1st, 2nd, 3rd, 4th, and 5th day after treatment. Concentrations of neomycin in muscles were determined by high performance liquid chromatography after SPE column extraction. The recovery rates of neomycin in muscle samples ranged from 78.4 to 84.3% for the concentration of neomycin 0.1 ㎎/㎏, and from 82.5 to 94.7% for the concentration of 0.2 ㎍/g. On the 1st day after treatment, neomycin concentrations were 0.125, 0.117, and 0.142 ㎍/g in muscles of olive flounder, rockfish, and red sea bream, respectively. Residue concentrations of all samples were decreased to lower than 0.1 ㎍/g, the detection limit, on day 2. According to the results of the present study, a withdrawal period of neomycin is proposed on 3 days after one day treatment with dipping administration at a dose of 140 ㎎/ton water to avoid the presence of excessive residues of the edible muscles of olive flounder, rockfish, and red sea bream.

여름철 참돔(Pagrus major)의 절식에 따른 생존과 생리적 반응

이정용,이정환,허준욱 한국수산과학회 2016 한국수산과학회지 Vol.49 No.5

A 60-day experiment was conducted to determine the effect of feeding and starvation on the survival, growth, and blood parameters of red sea bream, Pagrus major. The starved group was not fed during the first 32 days of the experiment and was then fed for 28 days. The growth rate of the starved fish group was significantly lower than that of the fed fish group. Starvation resulted in growth retardation and reductions in final body weight, growth rate, specific growth rate, and condition factor, whereas the fed fish group grew well and maintained a good condition. The growth rate of the starved group that was refed was higher than that of the fed group. Red blood cells, hematocrit, and hemoglobin showed no significant differences between the fed and starved groups. The cortisol and glucose levels of the fed group of juveniles were higher than those of the starved group. The cortisol levels of the starved group of adults were higher than those of the fed group, whereas the glucose levels of the starved group were lower than those of the fed group. These results suggest that the survival, growth, and hematological parameters used to reflect starvation and feeding in this study provide a useful index of physiological response and survival rate in red sea bream.

The first report of cathepsin A gene in a teleost (rock bream, <i>Oplegnathus fasciatus</i>): An investigation of immune functions upon infection with several pathogens

Kim, Ju-Won,Kong, Hee Jeong,Nam, Bo-Hye,Park, Jung Youn,Kim, Do-Hyung,Jeong, Ji-Min,Park, Chan-Il Elsevier 2017 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.76 No.-

<P><B>Abstract</B></P> <P>We isolated and characterised a cDNA encoding the lysosomal protective protein (serine protease) cathepsin A (CTSA) from rock bream (<I>Oplegnathus fasciatus</I>). The full-length rock bream CTSA (RbCTSA) cDNA (1814 bp) contains an open reading frame of 1419 bp that encodes 472 amino acids. Alignment of multiple CTSA protein sequences revealed that the active site serine and histidine residues were well-conserved among the other CTSA sequences. RbCTSA is highly expressed in the peripheral blood leukocytes, kidney, spleen, liver, intestine, gill, heart, brain, stomach, and eye. RbCTSA expression was also examined in several tissues, including whole kidneys and spleens, under bacterial and viral challenge. In general, all of the examined tissues that were infected with <I>Edwardsiella tarda</I>, <I>Streptococcus iniae</I>, or red sea bream iridovirus (RSIV) exhibited significant upregulation of RbCTSA expression compared to the controls. Our results reveal that RbCTSA may be involved in the immune responses of rock bream.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The cathepsin A (CTSA) cDNA was identified from a rock bream. </LI> <LI> The active site serine and histidine residues were well-conserved within the aligned sequence. </LI> <LI> RbCTSA gene was significantly expressed in the kidney, spleen, gill and intestine. </LI> <LI> RbCTSA upregulation was observed in response to pathogen infections. </LI> </UL> </P>

First description of programmed cell death10 (PDCD10) in rock bream (Oplegnathus fasciatus): Potential relations to the regulation of apoptosis by several pathogens

Kim, J.W.,Jeong, J.M.,Bae, J.S.,Cho, D.H.,Jung, S.H.,Hwang, J.Y.,Kwon, M.G.,Seo, J.S.,Baeck, G.W.,Park, C.I. Pergamon Press ; Elsevier Science 2016 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.55 No.-

<P>In this study, we isolated and characterized programmed cell death10 (PDCD10), which is known to be related to apoptosis, from rock bream (Oplegnathus fasciatus). The full-length rock bream PDCD10 (RbPDCD10) cDNA (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. Furthermore, multiple alignments revealed that the six of the a-helix bundles were well conserved among the other PDCD10 sequences tested. RbPDCD10 was significantly expressed in the liver, RBC (red blood cell), gill, intestine, trunk kidney and spleen. RbPDCD10 gene expression was also examined in several tissues, including the kidney, spleen, liver, and gill, under bacterial and viral challenges. Generally, all of the examined tissues from the fish that were infected with Edwardsiella tarda and the red sea bream iridovirus (RSIV) exhibited significant up-regulations of RbPDCD10 expression compared to the controls. However, RbPDCD10 expression exhibited dramatic down-regulations in all of the examined tissues following injections of Streptococcus iniae, which is major bacterial pathogen that is responsible for mass mortality in rock bream. Our results revealed that rock bream PDCD10 may be involved in the apoptotic regulation of rock bream immune responses. (C) 2015 Elsevier Ltd. All rights reserved.</P>