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para-Phenylenediamine처리에 따른 HaCaT 세포주의 세포변성효과 및 생존율
홍은설,송연숙,이근광 한국미용학회 2018 한국미용학회지 Vol.24 No.6
The purpose was to investigate the cytopathic effect and cell viability on cultivated HaCaT cell lines after processing for 24 hours and 48 hours through treatment with differing concentrations of PPD. The results are as fellows: Normal HaCaT cell lines grew normally in a cell cultivation flask, and were almond shaped. HaCaT cell lines that were exposed to the toxicity of PPD were generally inactive and showed signs of the cytopathic effect because of weak adhesion. Less than 50% cell viability was observed after 50 minutes in a group with 0.5% PPD, About 48% cell viability was observed after 10 minutes in a group with 1~3% PPD, and more than 45~40% cell viability was observed after 10 minutes in a group with 4~5% of PPD. The results due to the degree of cytotoxicity show that; The survival rate of cultivated cells after 48 hours increased after treatment for 10 minutes for a group with 0.5~1% PPD compared to cultivated cells treated for 24 hours. This suggests cells recovered little by little after 48 hours from a group with less than 1% of the treatment concentration for 10 minutes. But, they didn’t recover in the group with 2~5% of the treatment concentration. Therefore, the survival rate of HaCaT cells went down and cell viability went up after increasing the treatment concentration and treatment time in relation to the degree of cell viability from the PPD. Cell numbers increased because of the recovery of cells after 48 hours with treatment for 10 minutes from groups with 0.5% and 1% (54.93% survival rate in 0.5% PPD, 49.25% survival rate in 1% PPD). The cytotoxicity rate decreased with the lower concentration treatment and shorter treatment time.
1998년도 부산지역에서의 Influenza 바이러스의 분리
김영희,조경순 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
This study was performed to monitor the circulation of various influenza virus strains since influenza is one of the commonest respiratory disease in man, its causative virus has been the subjects of extensive research. The authors investigated the epidemics of influenza in Pusan in 1998. Influenza viruses have been isolated from patients with respiratory disease whose ages range from 1 to 68. Virus isolation from female was higher than male. The isolation of virus was mostly concentrated in December in 1998. The isolated virus showed strong cytopathic effect on MDCK cells and identified as influenza A/Sydney/05/97-like(H3N2) and influenza A/Beijing/262/95-like(H1N1). A negative staining of electron micrograph showed 130 ㎚ with H3N1 and 145 ㎚ with H1N1 in diameter, respectively.
1998년도 부산지역에서 Infuenza 바이러스의 분리
조경순 ( Kyung Soon Cho ) 대한임상검사과학회 1999 대한임상검사과학회지(KJCLS) Vol.31 No.2
This study is focused to monitor the circulation of various influenza virus strains since influenza is one of the commonest respiratory disease in man and its causative virus has been the subjects of extensive research. The authors investigated the epidemics of influenza from Pusan in 1998. Influenza virus has been isolated from patients with respiratoη disease suffered from ages 1 year old to 68 years old. Virus isolation from fema1e was higher than ma1e. The isolation of virus was most1y concentrated in December in 1998. The isolated virus showed strong cytopathic effect on MDCK cells and identified as influenza Aj Sydney j 05j97-like (H3N2) and influenza AjBeijingj262j95-1ike (H1N1). A negative staining of electronmicrograph showed 130nm with H3h H3N1 and 145nm with H1N1 in diameter, respectively.
부산지역 무균성 뇌막염 원인 바이러스의 분리 및 동정 : 1998년을 중심으로
김영희,정영기,김기순,지영미,윤재득,김병준,구평태,민상기,정구영,김만수,조경순 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
The incidence of aseptic meningitis infection is ensuing and threatening the health of children. Enteroviruses are the major agents of aseptic meningitis and identification of virus has been a clue to diagnosis and epidemiology. The outbreak of aseptic meningitis occurred in Pusan, 1998. Patients were concentrated from April through November. Children were more susceptible than adults. Among 306 cases of specimens from stool, throat swab tested, only 7.2% were positive on virus isolation, 12 cases from stool and 10 from throat, respectively. All isolated 7 serotypes of viruses represented cytopathic effect on cultured cells. Three types of echovirus 6, 25, 30 and coxsackievirus B2, B3, B4, B6 were identified by neutralizing anti body test. Isolated coxsackievirus and echovirus were observed by an electron microscope with negative staining.
Inhibitory Effect on Replication of Enterovirus 71 of Herb Methanol Extract
Choi, Hwa-Jung,Song, Jae-Hyoung,Ahn, Young-Joon,Kwon, Dur-Han The Korean Society for Applied Biological Chemistr 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.3
Anti-enterovirus 71 (EV 71) activities of fifteen herb plant species extracts were examined by SRB assay, among which Origanum vulgare and Rosmarinus officinalis (Anna Rosemary) extracts exhibited the activities with $IC_{50}$ of 8.28 and $8.17\;{\mu}g/mL$, respectively. Their 50% cytotoxicity concentrations ($CC_{50}$) were 691.89 and $1104.19\;{\mu}g/mL$, and the therapeutic indices were 83.56 and 135.15, respectively. Amantadine (positive control) showed anti-EV 71 activity with 50% inhibitory concentration and $CC_{50}$ of 4.46 and $145.22\;{\mu}g/mL$, respectively. Addition of the methanol extracts of O. vulgare and R. officinalis (Anna Rosemary) in EV 71-infected Vero cells strongly inhibited the formation of visible cytopathic effects without changing the normal morphology of the cells. These results indicate that methanol extracts of O. vulgare and R. officinalis (Anna Rosemary) may contain antiviral compound inhibiting the EV 71 replication.
Inhibitory Effect on Replication of Enterovirus 71 of Herb Methanol Extract
( Hwa Jung Choi ),( Jae Hyoung Song ),( Young Joon Ahn ),( Dur Han Kwon ) 한국응용생명화학회 2007 Journal of Applied Biological Chemistry (J. Appl. Vol.50 No.3
Anti-enterovirus 71 (EV 71) activities of fifteen herb plant species extracts were examined by SRB assay, among which Origanum vulgare and Rosmarinus officinalis (Anna Rosemary) extracts exhibited the activities with IC50 of 8.28 and 8.17 μg/mL, respectively. Their 50% cytotoxicity concentrations (CC50) were 691.89 and 1104.19 μg/mL, and the therapeutic indices were 83.56 and 135.15, respectively. Amantadine (positive control) showed anti-EV 71 activity with 50% inhibitory concentration and CC50 of 4.46 and 145.22 μg/mL, respectively. Addition of the methanol extracts of O. vulgare and R. officinalis (Anna Rosemary) in EV 71-infected Vero cells strongly inhibited the formation of visible cytopathic effects without changing the normal morphology of the cells. These results indicate that methanol extracts of O. vulgare and R. officinalis (Anna Rosemary) may contain antiviral compound inhibiting the EV 71 replication.
( Zu Bing Li ),( Jing Liu ),( Yi Fang Zhao ) 생화학분자생물학회 2005 BMB Reports Vol.38 No.1
GLPG (Ganoderma lucidum proteoglycan) was a bioactive fraction obtained by the liquid fermentation of the mycelia of Ganoderma lucidum, EtOH precipitation, and DEAE-cellulose column chromatography. GLPG was a proteoglycan with a carbohydrate: protein ratio of 10.4: 1. Its antiviral activities against herpes simplex virus type 1 (HSV 1) and type 2 (HSV-2) were investigated using a cytopathic inhibition assay. GLPG inhibited cell death in a dose-dependent manner in HSV-infected cells. In addition, it had no cytotoxic effect even at 2 mg/ml. In order to study the mode of action of the antiviral activity of GLPG, cells were treated with GLPG before, during, and after infection, and viral titer in the supernatant of cell culture 48 h post-infection was determined using a TCIDG_(50) assay. The antiviral effects of GLPG were more remarkable before viral treatment than after treatment. Although the precise mechanism has yet to be defined, our work suggests that GLPG inhibits viral replication by interfering with the early events of viral adsorption and entry into target cells. Thus, this proteoglycan appears to be a candidate anti-HSV agent.
Li, Zubing,Liu, Jing,Zhao, Yifang Korean Society for Biochemistry and Molecular Biol 2005 Journal of biochemistry and molecular biology Vol.38 No.1
GLPG (Ganoderma lucidum proteoglycan) was a bioactive fraction obtained by the liquid fermentation of the mycelia of Ganoderma lucidum, EtOH precipitation, and DEAE-cellulose column chromatography. GLPG was a proteoglycan with a carbohydrate: protein ratio of 10.4: 1. Its antiviral activities against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) were investigated using a cytopathic inhibition assay. GLPG inhibited cell death in a dose-dependent manner in HSV-infected cells. In addition, it had no cytotoxic effect even at 2 mg/ml. In order to study the mode of action of the antiviral activity of GLPG, cells were treated with GLPG before, during, and after infection, and viral titer in the supernatant of cell culture 48 h post-infection was determined using a $TCID_{50}$ assay. The antiviral effects of GLPG were more remarkable before viral treatment than after treatment. Although the precise mechanism has yet to be defined, our work suggests that GLPG inhibits viral replication by interfering with the early events of viral adsorption and entry into target cells. Thus, this proteoglycan appears to be a candidate anti-HSV agent.