촉칠(蜀漆)의 기원에 관한 문헌적 고찰

김재현 ( Chae-hyun Kim ),정종길 ( Jong-gil Jeong ) 대한상한금궤의학회 2009 대한상한금궤의학회지 Vol.1 No.1

Objective : The purpose of this study is to analyze the origin plants and the characteristics of the origin-plants of Chok-Chil. Method : To achieve the purpose of this study, bibliographies about Chok-Chil were examined. The examination was focused on the origin. Standing on this analysis the list and characteristics of the origin plants were presented. Results : 1. Chok-Chil(蜀漆) is the twig and leaf of Saxifragaceae Dichroa febrifuga Lour. 2. Sang-San(常山) is the root of Saxifragaceae Dichroa febrifuga Lour. 3. Chok-chil is toxic, and the effects are to make expectoration easy and to prevent further attack of malaria. Conclusions : Chok-Chil(蜀漆) is the twig and leaf of Saxifragaceae Dichroa febrifuga Lour.

노루오줌으로부터 Bergenin의 함량 분석 및 에탄올 추출물의자궁경부암세포 성장 억제 효과

김아현,노종현,우경완,강주은,심미옥,김민석,함성호,조현우 한국생약학회 2018 생약학회지 Vol.49 No.1

Astilbe rubra (AR) is a perennial, belongs to the Saxifragaceae family, it contains tannin and triterpene. AR has been used in republic korea to improve toxication, fever, pain and convulsion. Recently, number of natural products have been analyzed for potential pharmacological activities including anti-cancer, anti-obesity and anti-diabetic medication. Consequently, we investigated the growth inhibition effect of Astilbe rubra water extract (WAC), ethanol extract (EAC) and bergenin on Hela cell (human adenocarcinoma cell). From whole plant of A. rubra, bergenin was isolated by column chromatography and its structures were identified by 1H, 13C NMR and IT TOF-ESI MS. High extraction efficiency of bergenin was shown at 0.95% under 60 min reflux extraction with 50% MeOH. The MTS assay showed that EAC (ethanol extract) treatment increased cell death in a dose-dependent manner. Moreover, EAC treatment on Hela cell increased apoptotic cell death and caspase-3 activity. Results suggest that EAC has growth inhibition effect on Hela cells, but not WAC and bergenin. 500 μg/mL EAC treatment inhibited Hela cell at 60.2±1.5%.

Taxonomical Study of Chrysosplenium L. (Saxifragaceae) in Korea Based on Chemical Composition

Kim, Hyun-Jun,Jeong, Hea-Seok,Kang, Shin-Ho The Plant Resources Society of Korea 2013 한국자원식물학회지 Vol.26 No.6

Components extracted from 7 species and 18 populations of Chrysosplenium in Korea were compared and analyzed using GC-MS analysis. 57 components (${\geq}80%$ quality) were identified, of which neophytadiene, palmitic acid and phytol were found at all the taxa. Percentage composition of isolated extracts showed a clear difference in components type and GC-MS profile. On the basis of that result, data matrix was made and cluster analysis using UPGMA was conducted. From the result of cluster analysis, two groups were recombined; one with alternate leaves comprised C. japonicum in Ser. Alternifolia and C. flagelliferum in Ser. Flagellifera and the other with opposite leaves gradationally comprised C. psuedofauriei in Ser. Sinica, C. ramosum in Ser. Oppositifolia and C. sphaerospermum, C. valdepilosum, C. flaviflorum in Ser. Pilosa. These chemotaxonomic results agreed in general with those of existing studies on external morphology and molecular. In conclusion, chemical composition can be an useful characters in understanding the relation analysis among interspecific and intraspecific complex with the help of cluster analysis of 7 species and 18 populations of Chrysosplenium in Korea.

Chrysosplenium epigealum J.W.Han et S.H.Kang : A New Species of Chrysosplenium (Saxifragaceae) from Korea

Han, Jong-Won,Kang, Shin-Ho The Plant Resources Society of Korea 2012 한국자원식물학회지 Vol.25 No.3

A new species of Chrysosplenium (Saxifragaceae), C. epigealum J.W.Han & S.H.Kang is described from Mt. Seoraksan, Inje-gun, Gangwon-do, Korea. This new species is distinct from C. flaviflorum Ohwi, its closely relative species, in having calyx 2-2.5 mm long, pistils slightly shorter than calyx, filaments 2-3 times longer than anthers and stolons epigeal.

자생 숙은노루오줌의 종자발아에 미치는 환경조건과 화학적 처리의 영향

장보국,조주성,이철희 한국자원식물학회 2016 한국자원식물학회지 Vol.29 No.2

Factors affecting germination of seeds in the forms of various environment and chemical compounds. The present study was aim to produced effective seed propagation method of Astilbe koreana (Kom.) Nakai which had expected high value for the cut flower, ornamental and pharmaceutical material. Seed width and length ranged 0.62, 2.22 ㎜, respectively, and weight of thousand seeds was 40.5 ㎎. As result of imbibition test, showed moisture content of fresh seed (2.57%) increased rapidly by water-soaking treatment under 24 hours, recording to maximum value of 29.8%, and expansion of the seed coat was observed. Seed germination was the best at 15℃ and light conditions (40.8%) among temperature and light conditions treated. Percent germination of seeds was improved under the low (15, 20℃) than high temperature (25, 30℃). In addition, the seed was not germinated at dark condition regardless of temperature. Seeds of A. koreana thus seemed that it had low temperature germinability conditions. To improve germination rate, seeds were submerged in various concentrations of growth regulators such as GA3 and kinetin, and minerals as KNO3 and KCl. As a results, KNO3 treatment, regardless of concentrations, promoted germination compared to control. Especially, percent of germination (77.8%), germination energy (96.1%), mean germination time (11.3 days) and T50 (6.5 days) were effectively improved by treatment of KNO3 20 mM. Key words - Astilbe, Saxifragaceae, GA3, Kinetin, KNO3, KCl 본 연구는 절화, 관상 및 의약소재로 이용가치가 높은 자생 숙은노루오줌의 종자 번식법을 개발하고자 수행되었다. 연구 에 사용된 종자는 크기가 0.62×2.22 ㎜이며, 1,000립중은 40.5 ㎎으로 조사되었다. 종자의 기간별 수분흡수율을 조사한 결과, 2.57%였던 수분함량이 침지처리 24시간 만에 29.8%로 증가하 였으며, 종피의 팽창이 관찰되었다. 숙은노루오줌의 종자는 저 온요구성 광발아성으로 15℃, 명조건에서 최종발아율(40.8%) 이 가장 높았으며, 발아상의 온도가 높아질수록 발아율은 낮아 졌다. 암조건에서는 어느 온도조건에서도 전혀 발아되지 않았 다. 종자에 생장조절제 및 무기염류를 처리한 결과, KNO3는 농 도에 관계없이 무처리구에 비해 우수한 발아촉진 효과를 나타 내었다. 특히 KNO3 20 mM 처리구에서 최종발아율(77.8%)과 발아세(96.1%)가 향상되었으며, 평균발아소요일수(11.3일)와 T50 (6.5일)도 효과적으로 단축시킬 수 있었다.

Phytochemical Investigation of Tiarella polyphylla

Guanghai Shen,오세량,민병선,Joongku Lee,Kyung Seop Ahn,김영호,Hyeong-Kyu Lee 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.1

Seven flavonoids (1-7), two triterpenoids (8 and 9) and four steroids (10, 11, 12 and 13) were isolated from the whole plant of Tiarella polyphylla. Based on the physicochemical and spectroscopic analyses, their structures were identified as myricetin (1), astragalin (2), afzelin (3), quercitrin (4), myricitin (5), nicotiflorin (6), isoquercitrin (7), tiarellic acid (8), 3β-hydroxy- 20(29)lupen-27-oic acid (9), β-sitosterol-3-O-β-D-glucoside (10), stigmasterol-3-O-β-D–glucoside (11), β–sitosterol (12) and ergosterol endoperoxide (13). All 13 compounds, with the exception of tiarellic acid were isolated for the first time from T. polyphylla. In the anti-complementary assay, the steroids (12 and 13) exhibited potent activities; whereas, the flavonoids (1 to 7) showed weak or no activities, but even the triterpenoids (8 and 9) and steroidal saponins (10 and 11) evoked hemolysis.

Anticomplementary Activity of Oleanane-type Triterpenes from the Roots of Aceriphyllum rossii

민병선 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.6

To gain a better understanding of the anticomplement activity of triterpenoids, the complement activity of five unusual oleanane-type triterpenoids, bearing a carboxyl group at the C-29 position, were estimated against the classical pathway of a complementary system. The five triterpenoids were obtained from the roots of Aceriphyllum rossii (Saxifragaceae), and were determined to be aceriphyllic acids B-E (1-4) and K (5). Of the isolated compounds, compounds 1, 2 and 5 showed anticomplement activities, with IC50 values of 328.4, 77.5 and 348.6 μM, respectively, whereas, compounds 3 and 4 were inactive. This showed that a carboxyl group at the C-23 position and an α-configuration of a hydroxyl group at the C-3 position in the olean-29-carboxylic acid triterpenoids seemed to play an important role in the anticomplement activity of human serum against erythrocytes.

Compounds from the Aerial Parts of Aceriphyllum rossii and Their Cytotoxic Activity

Thi Thu Trang Tran,민병선,Thi Yen Nguyen,Manh Hung Tran,원권연,우미희 한국생약학회 2014 Natural Product Sciences Vol.20 No.3

The purification of the MeOH extracts from the aerial parts of Aceriphyllum rossii Engler (Saxifragaceae) using column chromatography furnished fourteen compounds (1 - 14). The chemical structures of the isolated compounds were determined using mainly nuclear magnetic resonance spectra and mass spectrometry. All the isolated compounds were tested for their cytotoxic activity against LH-60, MCF-7 and HeLa cancer cells in vitro using a MTT assay method. Compounds possessing an olean-triterpenoid skeleton entirely exhibit dose dependent cytotoxic activity. These findings partially confirmed the anticancer effect of this medicinal plant, suggesting a further study on the anticancer potential of the triterpenoid structure in compounds from this plant.

Cytotoxicity of Triterpenes Isolated from <i>Aceriphyllum rossii</i>

Lee, IkSoo,Yoo, Jae Kuk,Na, MinKyun,Min, Byung Sun,Lee, JongPill,Yun, Bong Sik,Jin, WenYi,Kim, HongJin,Youn, UiJung,Chen, Quan Cheng,Song, Kyung Sik,Seong, Yeon Hee,Bae, KiHwan The Pharmaceutical Society of Japan 2007 Chemical & pharmaceutical bulletin Vol.55 No.9

<P>Bioassay-guided fractionation of a MeOH extract of the whole plant of <I>Aceriphyllum rossii</I> (Saxifragaceae) led to the isolation of two new triterpenes, 3α,23-isopropylidenedioxyolean-12-en-27-oic acid (1) and 23-hydroxy-3-oxoolean-12-en-27-oic acid (2), together with six known triterpenes, 3-oxoolean-12-en-27-oic acid (3), 3α-hydroxyolean-12-en-27-oic acid (4), β-peltoboykinolic acid (5), aceriphyllic acid A (6), oleanolic acid (7), and gypsogenic acid (8). The structures of these compounds were elucidated on the basis of physicochemical and spectroscopic analyses. These compounds were evaluated for <I>in vitro</I> cytotoxicity against the K562 and HL-60 cell lines. Olean-12-en-27-oic acid derivatives (1—6) exhibited considerable cytotoxicity against K562 and HL-60 cell lines with IC<SUB>50</SUB> values ranging from 12.2 to 28.7 μ<SMALL>M</SMALL> and from 12.1 to 25.8 μ<SMALL>M</SMALL>, respectively.</P>

Determination of Bergenin in Different Parts of Bergenia ciliata using a Validated RP-HPLC Method

Ejaz Ali,Khalid Hussain,Nadeem Irfan Bukhari,Najma Arshad,Amjad Hussain,Nasir Abbas,Sohail Arshad,Sajida Parveen,Naureen Shehzadi,Shaista Qamar,Abida Qamar 한국생약학회 2021 Natural Product Sciences Vol.27 No.1

Bergenia ciliata (Family: Saxifragaceae) is a folklore remedy for the treatment of various ailments in Asian countries. Bergenin (1) has been isolated as an active constituent in many studies, however, the amount of bergenin has not been determined in all parts of the plant. A simple RP-HPLC method was developed to determine the amount of bergenin in methanol extracts of leaves, rhizomes and roots of the plant. Separation was achieved on an Agilent Eclipse XDB-C18 column maintained at 25 oC using isocratic solvent system (water: methanol: acetic acid; 62.5:37:0.5 v/v/v) adjusted at pH 2 0 at a flow rate of 1.0 mL/min. and detected at 275 nm. Correlation coefficient (0.9952) showed linearity of concentration (5-200 μg/mL) and response. The values of LOD (0.00947 μg/mL) and LOQ (0.02869 μg/mL) indicated that method was sensitive. The recovery of bergenin was 99.99-100% indicating accuracy of method. The methanol extract of rhizomes contained higher amount of bergenin (19.4%) than roots (9.2%) and leaves (6.9%). It is concluded that methanol extract of rhizomes is a better source of bergenin than other parts of the plant. The findings are useful for standardization of bergenin containing extracts and herbal preparations.