장미과 식물 추출물의 생물학적 활성

서정민 ( Jeong Min Seo ),안정엽 ( Jeung-youb Ahn ) 대한화장품학회 2004 대한화장품학회지 Vol.30 No.4

장미과 식물(에탄을 추출)의 항산화력을 측정하기 위하여 1,1-diphenyl-2-picrylhydrazyl (DPPH) 자유 라디칼 생성 시스템을 이용하여 하였다. 또한 산화 환원 과정을 통하여 피부의 과잉 색소 생성에 관련된 멜라닌 생합성 과정의 조절 가능성을 조사하였다. 장미과 식물중에서 Prunus sargentii, Rubus coreanus, Chaenomeles sinensis, Photinia glabra와 Pyrus pyrifolia은 생합성 과정에서 dopa [3-(3,4-dihydroxyphenyl) alanine]를 도파크롬으로 변환시켜 주는 tyrosinase 저해 효과를 나타내었다. MTT 실험법은 장미과 에탄올 추출물의 사람 섬유아세포에 미치는 독성정도를 실험하기 위해 사용되었다. 장미과 식물중 특히 Prunus sargentii의 껍질, Phorinia glabra의 껍질과 나무 그리고 Chaenomeles sinenis의 잎, 껍질, 나무 모든 부분에서 mushroom tyrosinase에 대해 100 ㎍/mL에서 50% 이상의 저해 활성을 보였으며, 10 ㎍/mL 농도에서 강한 라디칼 소거 효과를 나타내었다. 또한 이들 추출물은 사람 섬유아 세포에 대해 높은 생존율을 나타냄으로써 멜라닌 형성 과정을 조절할 수 있을 것으로 기대한다. DPPH radical-generating system was used to evaluate the antioxidant properties of the Rosaceae. The inhibitory effects of ethanolic extracts from Rosaceae plants were investigated on melanin biosynthesis which is closely related to hyperpigmentation. Of the Rosaceae extracts, Prunus sargentii, Rubus coreanus, Chaenomeles sinensis, Photinia glabra and Pyrus pyrifolia showed a potent inhibition of tyrosinase, the enzyme which converts 3-(3,4-dihydroxyphenyl) alanine (dopa) to dopachrome in the melanin biosynthetic process. Furthermore, MMT assay was used to check the cytotoxicity of extracts on the human foreskin fibroblast cell line, Hs68. Among the Rosaceae, bark of Prunus sargentii, bark wood of Photinia glabra and all parts or Chaenomeles sinensis showed more than 50% inhibition of mushroom tyrosinase activity at 100 ㎍/mL and more than 80% of strong DPPH radical-scavenging activity at 10 ㎍/mL. In audition to, they had no cytotoxic activity on Hs68. These results suggest that these extracts might be except a controler in pigmentation.

韓國産 薔薇科 植物에 관한 本草學的 硏究 I

정종길,황상욱,송호준,신민교 한국전통의학연구소 2004 한국전통의학지 Vol.14 No.1

For the purpose of developing Korean Herbalogy with the plants of Rosaceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been thoroughly investigated and the results obtained as follows: 1. There were totaled to 36 genera and 262 in Korea and among them medicinal plants are 27 genera, 117species, some 44% in total, but the number of species may be added because of the similiar plants. 2. According to sum of 262 species in Rosaceae family, they were classified into Agrimonia genera 3, Potentilla genera 26, Prunus genera 48,pyrus genera 17, Rosa genera 24, Rubus genera 28, Sorbaria genera 17, Spiraea genera 20. Thus it was noticed that Prunus genera was the main kind in total. 3. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Rosaceae family were classified as Herba 24, Radix 43, Foilum 31, Fructus 48, Semen 11, cortex 14, Lignum, Ramulus;12 each Flos 3, pericarpium 3, resina 2, the others 6. so Fructus is 24% of all and it is the most applied in the clinical treatments. 4. According to nature and flavour of medicinal plants, they were classified into balance 84, warmth, heat; 44 each, cold, cool; 35 each bitter taste 79, sweet taste 74, sour taste 61 thus it was noticed that balance and bitter taste is the main in nature and flavour of medicinal plants 5. According to meridian tropism of herbs they were classified into liver meridian 50 lung meridian 28, stomach meridian 22, spleen meridian 20, heart meridian 19 thus it was noticed that liver meridian is the main kind 6. According to the Properties and Principal curative action, they were classified into drugs for treating detoxicant 72, drugs for treating febrifugal 55, drugs for invigorating blood circulation 53, drugs for removing dampness 46, drugs for expelling wind and drugs to stop bleeding 38 each, drugs to cure tramatic swelling and drugs for relieving pain 36 each. Thus it was noticed that drugs for treating detoxicant and febrifugal was the main kind 7. It was researched that toxic drugs include minor toxin were 10 kinds From this result, it was revealed that Fructus was the main kind in the plants for medical purpose of Rosaceae. and toxic plants was slightly poisonous that it will be used for clinical treatments more easily. It is considered that many experiments and clinical approaches must be continued to use Rosaceae plants widely.

韓國産 薔薇科 植物에 관한 本草學的 硏究 1

정종길,황상욱,송호준,신민교 한국전통의학연구소 2004 한국전통의학지 Vol.14 No.1

For the purpose of developing Korean Herbalogy with the plants of Rosaceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been thoroughly investigated and the results obtained as follows: 1. There were totaled to 36 genera and 262 in Korea and among them medicinal plants are 27 genera, 117species, some 44% in total, but the number of species may be added because of the similiar plants. 2. According to sum of 262 species in Rosaceae family, they were classified into Agrimonia genera 3, Potentilla genera 26, Prunus genera 48,pyrus genera 17, Rosa genera 24, Rubus genera 28, Sorbaria genera 17, Spiraea genera 20. Thus it was noticed that Prunus genera was the main kind in total. 3. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Rosaceae family were classified as Herba 24, Radix 43, Foilum 31, Fructus 48, Semen 11, cortex 14, Lignum, Ramulus;12 each Flos 3, pericarpium 3, resina 2, the others 6. so Fructus is 24% of all and it is the most applied in the clinical treatments. 4. According to nature and flavour of medicinal plants, they were classified into balance 84, warmth, heat; 44 each, cold, cool; 35 each bitter taste 79, sweet taste 74, sour taste 61 thus it was noticed that balance and bitter taste is the main in nature and flavour of medicinal plants 5. According to meridian tropism of herbs they were classified into liver meridian 50 lung meridian 28, stomach meridian 22, spleen meridian 20, heart meridian 19 thus it was noticed that liver meridian is the main kind 6. According to the Properties and Principal curative action, they were classified into drugs for treating detoxicant 72, drugs for treating febrifugal 55, drugs for invigorating blood circulation 53, drugs for removing dampness 46, drugs for expelling wind and drugs to stop bleeding 38 each, drugs to cure tramatic swelling and drugs for relieving pain 36 each. Thus it was noticed that drugs for treating detoxicant and febrifugal was the main kind 7. It was researched that toxic drugs include minor toxin were 10 kinds From this result, it was revealed that Fructus was the main kind in the plants for medical purpose of Rosaceae. and toxic plants was slightly poisonous that it will be used for clinical treatments more easily. It is considered that many experiments and clinical approaches must be continued to use Rosaceae plants widely.

Phylogenetic analysis of PISTILLATA sequences in Neillia (Rosaceae)

오상훈 한국식물학회 2013 Journal of Plant Biology Vol.56 No.3

Putative PISTILLATA genes were generated in nine species of Neillia, to examine the phylogenetic relationships among the species, and to test the hypothesis of hybrid origin within the genus. The PI genes determined in Neillia have two introns in the I-box region, which is consistent with PI genes in other Rosaceae. Phylogenetic analyses of the I-box region, including the introns, indicated that the species formerly classified in Stephanandra were nested within Neillia, supporting the taxonomic merger of the two genera. The PI data do not have a sufficiently strong signal to reject the hypothesis that Stephanandra is a hybrid in origin. The PI data, in conjunction with nuclear LEAFY, ribosomal DNA, and chloroplast DNA data, suggest that N. affinis might have been derived from hybridization between N. thibetica and N. gracilis. The phylogenetic position of N. affinis in the N. thibetica clade is supported by the PI and rDNA data, whereas N. affinis is also supported as a sister to N. gracilis in the LEAFY and cpDNA data. The pattern of phylogenetic placements of N. affinis in two different clades in two different sets of data suggests that the genome of the species might be comprised of a combination of the putative parental species.

The complete plastid genome and nuclear ribosomal transcription unit sequences of Spiraea prunifolia f. simpliciflora (Rosaceae)

최정진,김원희,박지영,강종수,양태진 한국식물분류학회 2023 식물 분류학회지 Vol.53 No.1

Spiraea prunifolia f. simpliciflora Nakai is a perennial shrub widely used for horticultural and medicinal purposes. We simultaneously obtained the complete plastid genome (plastome) and nuclear ribosomal gene transcription units, 45S nuclear ribosomal DNA (nrDNA) and 5S nrDNA of S. prunifolia f. simpliciflora, using Illumina short-read data. The plastome is 155,984 bp in length with a canonical quadripartite structure consisting of 84,417 bp of a large single-copy region, 18,887 bp of a short single-copy region, and 26,340 bp of two inverted repeat regions. Overall, a total of 113 genes (79 protein-coding genes, 30 tRNAs, and four rRNAs) were annotated in the plastome. The 45S nrDNA transcription unit is 5,848 bp in length: 1,809 bp, 161 bp, and 3,397 bp for 18S, 5.8S, and 26S, respectively, and 261 bp and 220 bp for internal transcribed spacer (ITS) 1 and ITS 2 regions, respectively. The 5S nrDNA unit is 512 bp, including 121 bp of 5S rRNA and 391 bp of intergenic spacer regions. Phylogenetic analyses showed that the genus Spiraea was monophyletic and sister to the clade of Sibiraea angustata, Petrophytum caespitosum and Kelseya uniflora. Within the genus Spiraea, the sections Calospira and Spiraea were monophyletic, but the sect. Glomerati was nested within the sect. Chamaedryon. In the sect. Glomerati, S. prunifolia f. simpliciflora formed a subclade with S. media, and the subclade was sister to S. thunbergii and S. mongolica. The close relationship between S. prunifolia f. simpliciflora and S. media was also supported by the nrDNA phylogeny, indicating that the plastome and nrDNA sequences assembled in this study belong to the genus Spiraea. The newly reported complete plastome and nrDNA transcription unit sequences of S. prunifolia f. simpliciflora provide useful information for further phylogenetic and evolutionary studies of the genus Spiraea, as well as the family Rosaceae.

쉬땅나무속(장미과-조팝나무아과)의 미기록 식물: 좀쉬땅나무

송준호,장진성,홍석표 한국식물분류학회 2010 식물 분류학회지 Vol.40 No.3

We report an unrecorded species of the genus Sorbaria (Rosaceae) in Korea, namely Sorbaria kirilowii (Regel & Tiling) Maxim. This plant was discovered both on Pocheon-si, Naecheon-myeon and Yeoju-gun, Buknae-myeon in Gyeonggi Province. It is distinguishable from Sorbaria sorbifolia (L.) A. Braun var. stellipila Maxim. by characteristics such as presence of hair on leaf and rachis, morphology of inflorescence, length and width of petal, shape of sepal, number and length of stamens, presence of hair upon and size of follicles. This taxon was given the Korean common name ‘Jom-swi-ttang-na-mu’ based on the small size of floral characters. 경기도 포천시 내천면과 여주군 북내면에서 발견된 우리나라 장미과 내의 미기록 종인 Sorbaria kirilowii (Regel & Tiling) Maxim.을 확인하여 보고하고자 한다. 본 종은 근연분류군인 쉬땅나무(Sorbaria sorbifolia (L.) A. Braun var. stellipila Maxim.)에 비해서 잎과 화경에 털이 없고, 원추화서는 넓게 퍼져 아래로 처지며, 꽃은 5−8mm로 작고, 꽃받침은 반원형, 둔두이며, 수술의 수가 적고, 비교적 짧으며, 골돌은 작고 털이 없다는 점에서 구별된다. 국명은 꽃과 열매의 크기가 쉬땅나무에 비해 작은 특징을 고려하여 ‘좀쉬땅나무’로 명명하였다.

Determination of Fire Blight Susceptibility on Wild Rosaceae Plants in Korea by Artificial Inoculation

Park In Woong,Song Yu-Rim,Oh Eom-Ji,Kim Yoel,Hwang In Sun,Jeon Mi Jin,Ahn Chorong,Kim Jin-Suk,Kim Soonok,오창식 한국식물병리학회 2023 식물병연구 Vol.29 No.1

The fire blight caused by Erwinia amylovora (Ea) is a devastating disease of Rosaceae plants, including commercially important apple and pear trees. Since the first report in Korea in May 2015, it has been spreading to neighboring regions gradually. Host plants can be infected by pollinators like bees, rainfall accompanied by wind, and cultural practices such as pruning. Many studies have revealed that wild Rosaceae plants such as Cotoneaster spp., Crataegus spp., Pyracantha spp., Prunus spp., and Sorbus spp. can be reservoirs of Ea in nature. However, wild Rosaceae plants in Korea have not been examined yet whether they are susceptible to fire blight. Therefore, the susceptibility to fire blight was examined with 25 species in 10 genera of wild Rosaceae plants, which were collected during 2020–2022, by artificial inoculation. Bacterial suspension (10⁸ cfu/ml) of Ea type strain TS3128 was inoculated artificially in flowers, leaves, stems, and fruits of each plant species, and development of disease symptoms were monitored. Moreover, the presence of Ea bacteria from inoculated samples were checked by conventional polymerase chain reaction. Total 14 species of wild Rosaceae plants showed disease symptoms of fire blight, and Ea bacteria were detected inside of inoculated plant parts. These results suggest that wild Rosaceae plants growing nearby commercial apple and pear orchards in Korea can be Ea reservoirs, and thus they should be monitored regularly to minimize the damage by Ea infection and spreading.

Genome-wide identification of the HKT genes in five Rosaceae species and expression analysis of HKT genes in response to salt-stress in Fragaria vesca

Shichuang Zhang,Yuexia Tong,Yingjun Li,Zong‑Ming Cheng,Yan Zhong 한국유전학회 2019 Genes & Genomics Vol.41 No.3

Introduction The high-affinity potassium transporter (HKT) gene family is implicated in the regulation of response to salt stress in different plants. Yet, knowledge remains very limited for them in the Rosaceae species. The whole genome sequences of the five Rosaceae species, including Fragaria vesca (woodland strawberry), Malus domestica (apple), Pyrus communis (pear), Prunus mume (mei) and Prunus persica (peach), give the opportunity to uncover the evolutionary pattern of the HKT genes among Rosaceae genomes. Objectives The primary objective of this study was to identify the HKT genes in five Rosaceae species and understand the potential biological functions of the HKT genes in response to salt stress in Fragaria vesca. Methods In this study, the HKT genes in five Rosaceae species were identified by bioinformatics. The phylogenetic tree was constructed and Ka and Ks values were calculated by MEGA 5.0. Genetic mapping of these genes were processed by MapInspect software. In addition, the physico-chemical properties, the cis-acting elements, the intron/exon structures, and the conserved domain motifs of these genes were analyzed and predicted by the websites of ProtParam, Pfam, PlantCARE, GSDS, MEME, and Motif Scan. Results In the present study, a total of 12 HKT genes were identified from five Rosaceae species. In the phylogenetic tree, orthologous genes were clustering together rather than paralogous genes and PcHKTs underwent two species-specific duplications before the divergence of pear and apple.. Furthermore, various expression levels of FvHKT genes revealed that woodland strawberry HKT genes are associated in responding to the salt stress. The activities of ROS-scavenging enzymes, superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were also measured in different treatments of salt-stressed woodland strawberry leaves, which suggested that the three enzymes may also take part in response to salt stress. Conclusion The Ka/Ks ratio demonstrated that purifying selection played a major role in the evolutionary processes of HKT genes among the Rosaceae species, and FvHKT genes are involved in response to salt stress.

Genomics and bioinformatics resources for translational science in Rosaceae

Sook Jung,Dorrie Main 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.2

Recent technological advances in biologypromise unprecedented opportunities for rapid and sustainableadvancement of crop quality. Following this trend,the Rosaceae research community continues to generatelarge amounts of genomic, genetic and breeding data. These include annotated whole genome sequences, transcriptomeand expression data, proteomic and metabolomicdata, genotypic and phenotypic data, and genetic andphysical maps. Analysis, storage, integration and disseminationof these data using bioinformatics tools and databasesare essential to provide utility of the data for basic,translational and applied research. This review discussesthe currently available genomics and bioinformaticsresources for the Rosaceae family.

솜양지꽃 뿌리로부터 스테로이드와 트리테르페노이드 성분의 분리

박희준,이경태,박종희 한국생약학회 2007 생약학회지 Vol.38 No.4

compounds (1-3) were isolated from the roots of Potentilla discolor (Rosaceae). The structure of compounds1-3 were elucidated as stigmast-5-en-3-ol (β-sitosterol, 1), 2,19α-dihydroxy-2-oxo-urs-1,12-dien-28-oic acid (fupenjic acid, 2),3-O-β-D-glucopyranosylstigmast-5-en-3-ol (β-sitosterol β-D-glucopyranoside, 3) based on physical and spectroscopic data.13C-NMR assignments were completed by 2D-NMR technique. The three compounds were firstly isolated from Potentilla dis-color.Key wordsPotentilla discolor, Rosaceae, steroid, fupenjic acid, 13C-NMR..... ... (Rosaceae). ... ... ...... .. 15-30 cm.. , . ... ..... ... .