가교화 찹쌀전분의 물리화학적 성질 및 유과제조 특성

유철(Chul Yu),최현욱(Hyun-Wook Choi),김종태(Chong-Tai Kim),안순철(Soon-Cheol Ahn),최성원(Sung-Won Choi),김병용(Byung-Yong Kim),백무열(Moo-Yeol Baik) 한국식품과학회 2007 한국식품과학회지 Vol.39 No.5

유과 제조 시 제조 시간을 단축할 수 있는 소재를 개발하기 위하여 찹쌀전분을 POCl3와 반응시켜 가교화 찹쌀전분을 제조하고 이들의 물리화학적 특성을 연구하였다. 가교화 찹쌀전분의 치환율은 0.04-2.73으로 나타났으며, 팽윤력은 일반 찹쌀전분에 비해 약간 증가되는 경향을 보였으며 용해도는 일반 찹쌀전분보다 낮은 경향을 보였다. 등온흡습곡선은 가교화에 따른 유의적인 차이를 보이지 않았다. 전분의 pasting 특성을 검토한 결과, 호화개시온도는 가교화에 따른 변화가 없는 것으로 나타났으며, 최대점도, holding strength, final viscosity, setback은 일반 찹쌀전분보다 높아지는 결과를 나타냈다. 열적특성은 호화개시온도, 최대호화온도, 호화종결온도 그리고 호화엔탈피 모두 각 전분간에 유의적인 차이가 나타나지 않았다. X-ray 회절 분석 결과로 볼 때, 가교화 찹쌀전분 및 일반 찹쌀전분 모두 A형의 결정 형태를 나타내었고, 상대적 결정화도의 차이가 나타나지 않는 것으로 보아 가교화가 찹쌀전분의 결정형영역에는 영향을 주지 않는 것으로 보여진다. 가교화 찹쌀전분을 사용하여 제조한 유과의 경우 일반 찹쌀을 장시간 침지한 후 제조한 시료와 비슷한 팽화율을 나타내었으며 시료의 변색 반응이 나타나지 않는 것으로 보아 유과 제조용 소재로서 적합한 것으로 판단되었다. In this study, waxy rice starch was chemically modified using phosphorous oxychloride (POCl₃, 0.002-0.008%). Then the physicochemical properties of resulting cross-linked waxy rice starches were investigated in order to reduce the steeping time of Yukwa (a Korean oil-puffed rice snack) processing. The swelling powers of the cross-linked waxy rice starch samples were higher than the native waxy rice starch at temperatures above 60℃, and their increases were proportional to the POCl₃ concentration. The solubility of the cross-linked waxy rice starch was lower (1.6-3.4%) than the native waxy rice starch (2.7-6.1%). However, the moisture sorption isotherm of the cross-linked waxy rice starch was not significantly different from the native waxy rice starch. The rapid visco analyzer (RVA) pasting temperatures (65.4-67℃) of the cross-linked waxy rice starch were lower than those of the native starch (67℃). The RVA peak viscosities (287-337 RVU) of the cross-linked waxy rice starch were higher than that of native starch (179 rapid visco units (RVU)), and increased with increasing POCl₃ concentration. For the differential scornning calorimeter thermal characteristics, although Tc shifted toward higher temperatures with cross-linking, the To, Tp, and amylopectin melting enthalpy of the cross-linked waxy rice starch showed no differences compared to the native waxy rice starch. The X-ray diffraction patterns of both the native and cross-linked waxy rice starches showed typical A-type crystal patterns, suggesting that cross-linking mainly occurs in the amorphous regions of starch granules. Therefore, the cross-linking reaction did not change the crystalline region, but altered the amorphous region of the waxy rice starch molecules, resulting in changes of solubility and RVA pasting properties in the cross-linked waxy rice starch. In summary, since cross-linked waxy rice starch has a high puffing efficiency and no browning reaction, it may be applicable for Yukwa processing without a long steeping process.

가교화 후 하이드록시프로필화한 복합변성 쌀전분의 이화학적 특성

최현욱 ( Hyun Wook Choi ),홍사훈 ( Sa Hoon Hong ),최성원 ( Sung Won Choi ),김창남 ( Chang Nam Kim ),유승석 ( Seung Seok Yoo ),김병용 ( Byung Yong Kim ),백무열 ( Moo Yeol Baik ) 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.4

Physicochemical properties of dual-modified rice starches, cross-linked (with POCl3) and hydroxypropylated (with propylene oxide) rice starches, were studied. Rice starch was cross-linked using POCl3 (0.005%, 0.02% (v/w)) at 45oC for 2 hr and then hydroxypropylated using propylene oxide (2%, 6%, 12% (v/w)) at 45oC for 24 hr, respectively. Swelling power, solubility, thermal properties (DSC) and pasting properties (RVA) of cross-linked and hydroxypropylated (CLHP) rice starches were determined. Swelling power of CLHP rice starch increased at relatively lower temperature than native rice starch. Solubility of CLHP rice starch was lower than that of native rice starch. Peak viscosity of CLHP rice starch was lower than that of native starch while holding strength and final viscosity were increased with modification. Breakdown value was lower and setback value was higher than native rice starch. DSC thermal transitions of CLHP rice starch shifted toward lower temperature. Amylopectin-melting enthalpy of CLHP rice starch decreased, whereas it was not affected by the amount of POCl3.

쌀 전분 첨가 옥수수, 감자, 고구마 전분 및 밀가루의

이향미,이영택 한국산업식품공학회 2013 산업 식품공학 Vol.17 No.3

가공식품의 원료로 널리 사용되고 있는 옥수수, 감자, 고구마 전분에 멥쌀 및 찹쌀로부터 분리한 전분을 일부(10-30%) 대체하여 신속점도측정기(RVA)로 호화특성을 조사하였다. 쌀 전분은 호화개시온도가 옥수수, 감자, 고구마전분에 비해 낮았고 페이스트 점도가 타 전분에 비해 낮은 경향이었다. 멥쌀 전분의 호화개시온도는 찹쌀 전분에 비해높았으며 최고점도, breakdown이 보다 높게 나타났다. 옥수수 전분에 멥쌀 및 찹쌀 전분을 10-30% 대체하여 RVA 호화양상을 측정한 결과 쌀 전분의 첨가는 RVA 최고점도, trough 점도를 증가시켰으나 최종점도에는 큰 증가세를 보이지 않아 setback은 다소 감소하는 경향이었다. 찰옥수수 전분에 쌀 전분의 첨가는 옥수수 전분과는 달리 RVA 최고점도를 감소시켰으며 최종점도는 증가세를 나타내 setback이 증가하는 것으로 나타났다. 감자 전분에 쌀 전분의 첨가는 최고점도, trough 점도, 최종점도를 다소 감소시켰으나 여전히 높은 점도를 유지하는 것으로 나타났으며setback 역시 여전히 낮게 유지됨을 보여주었다. 고구마 전분에 쌀 전분의 첨가는 전분 paste의 최고점도, trough, 최종점도가 감소하는 결과를 초래하였다. 밀가루에 쌀 전분을 대체시에 멥쌀 전분은 밀가루의 페이스트 점도를 증가시키는 반면에 찹쌀 전분은 전반적으로 점도를 감소시키는 것으로 나타났다. Rapid visco-analyzer (RVA) was used to evaluate the pasting properties of selected starches; i.e., corn, potato, and sweet potato starches and wheat flours substituted with rice starches isolated from normal and waxy rice samples. The gelatinization temperature and pasting viscosities of rice starch were lower than those of the corn, potato, and sweet potato starches. The peak and trough viscosities of normal corn starch increased as substitution levels of normal and waxy rice starches increased, while setback values tended to decrease. Contrary to normal corn starch, the peak viscosity of waxy corn starch decreased as substitution levels of rice starch increased, while final and setback viscosities increased. The peak viscosity of potato starch, showing the highest value among the starches, slightly decreased with increasing levels of rice starch. However, compared to the other starches, the pasting viscosity ofpotato starch substituted with rice starch was the highest and its setback value was the lowest. The pasting viscosityof sweet potato starch decreased as substitution levels of rice starch increased. Incorporating normal rice starch increased the pasting viscosities of wheat flour, while waxy rice starch resulted in lowered flour pasting viscosities.

하이드록시프로필화 쌀 전분의 이화학적 특성

최현욱,구혜진,김종태,황성연,김동섭,최성원,허남윤,백무열,Choi, Hyun-Wook,Koo, Hye-Jin,Kim, Chong-Tai,Hwang, Seong-Yun,Kim, Dong-Seob,Choi, Sung-Won,Hur, Nam-Youn,Baik, Moo-Yeol 한국식품과학회 2005 한국식품과학회지 Vol.37 No.1

쌀 전분에 propylene oxide(PO)를 단계별(전분 공형분 대비 2-12%)로 20시간과 24시간 반응시켜 하이드록시프로필화 쌀 전분을 제조하고, 변성된 쌀 전분의 용해도, 팽윤력, RVA, DSC 특성을 연구하였다. 팽윤력은 일반 쌀 전분 보다 낮은 온도에서 증가되기 시작하였으나 높은 경향을 보였으며, 하이드록시프로필화 쌀 전분이 일반 쌀 전분보다 완만한 상승을 나타내었다. 용해도는 하이드록시프로필화 쌀 전분이 일반 쌀 전분보다 낮은 결과를 나타내었고 PO 함량이 높을수록 높아지는 결과를 보였다. RVA 분석결과 PO 함량이 높을수록 pasting temperature와 peak time이 낮아졌고 최고 점도는 하이드록시프로필화 쌀 전분이 일반 쌀 전분보다 낮고 holding strength는 높은 경향을 나타내고 breakdown의 경우 반응시간이 24시간이 20시간보다 낮은 경향을 보였으며 Setback은 24시간 반응시킨 처리구들이 일반 쌀 전분보다 낮은 것으로 나타났다. DSC분석결과 PO 함량이 높을수록 To, Tp, Tc, ${\Dalta}H$가 감소하였다. 따라서 하이드록시프로필화 쌀 전분의 경우 전분입자내의 intemal bond가 하이드록시프로필기에 의해 약해져서 호화가 쉽게, 즉 낮은 온도에서 일어나는 것으로 판단되었고 최대 팽윤력, breakdown에서 옥수수 전분과는 다른 결과를 나타내어 전분의 종류에 따라 같은 변성 처리라고 하더라도 다른 결과를 나타낼 수 있다는 사실이 확인되었다. Physicochemical properties of hydroxypropylated rice starches were investigated. Swelling power of hydroxypropylated rice starch increased at relatively lower temperature than native rice starch. Solubility of hydroxypropylated rice starch was lower (1.9-13.4%) than that of native rice starch (2.2-13.8%), and increased with increasing amount of propylene oxide. Pasting temperature ($66.2-70.8^{\circ}C$) and peak viscosity (2,843-3,395cp) of hydroxypropylated rice starch were lower than those of native starch ($71.6^{\circ}C,\;3,976\;cp$) and decreased with increasing amount of propylene oxide, regardless of reaction time. DSC thermal transitions of hydroxypropylated rice starches shifted toward lower temperature. Amylopectin-melting enthalpy of hydroxypropylated rice starch decreased (11.8-9.8J/g) with increasing amount of propylene oxide and was lower than that of native starch (11.9 J/g). These results indicate hydroxypropylation lowered swelling power and gelatinization temperatures of rice starch, because internal bonds of rice starch molecules were sterically weaken by substituted hydroxypropyl groups.

가교화 후 하이드록시프로필화한 복합변성 쌀전분의 이화학적 특성

최현욱,홍사훈,최성원,김창남,김병용,백무열,유승석 한국산업식품공학회 2011 산업 식품공학 Vol.15 No.4

POCl3 농도(0.005%, 0.02%)를 달리한 가교화 쌀전분에 PO(2%, 6%, 12%)를 처리하여 가교화 후하이드록시프로필화(CLHP) 쌀전분을 제조하고, 변성된 CLHP 쌀전분의 용해도, 팽윤력, RVA, DSC 특성을 연구하였다. 팽윤력은 일반 쌀전분 보다 다소 낮은 온도에서 일찍 증가되기 시작하였으나 그 이후에 모두 낮은 수치를 보여 CLHP 쌀전분이 일반 쌀전분보다 완만한 상승을 나타내었고 용해도는 CLHP 쌀전분이 일반 쌀전분보다 전체적으로 낮은 결과를 나타내었다. RVA 분석결과 CLHP 쌀전분의 최고점도는 일반 쌀전분보다 낮아졌고 holding strength와 final viscosity는 높은 경향을 나타내었고, breakdown은 낮아지고 setback 은 높은 것으로 나타났다. DSC 분석 결과 CLHP 쌀전분의 To, Tp, Tc가 일반 쌀전분보다 낮게 나타났으며, ΔH 역시 작은 값을 보였으며 POCl3 함량에 따른 차이, 즉 가교화에 의한 차이는 크지 않았다. 따라서 가교화 후 하이드록시프로필화 한 쌀전분의 경우 RVA점도 특성은 가교화에 의한 영향이 크고 하이드록시프로필화에 따른 영향이 작다고 볼 수 있었으며, DSC열적특성은 가교화에 의한 영향으로 하이드록시프로필화가 원활하지 않았던 것으로 생각된다. 즉 인산기에 의해 강해진 전분 입자에 의해 하이드록시프로필기의 치환이 덜 일어난 것으로 판단된다. Physicochemical properties of dual-modified rice starches, cross-linked (with POCl3) and hydroxypropylated (with propylene oxide) rice starches, were studied. Rice starch was cross-linked using POCl3 (0.005%, 0.02% (v/w)) at 45oC for 2 hr and then hydroxypropylated using propylene oxide (2%, 6%, 12% (v/w)) at 45oC for 24 hr, respectively. Swelling power, solubility, thermal properties (DSC) and pasting properties (RVA) of cross-linked and hydroxypropylated (CLHP) rice starches were determined. Swelling power of CLHP rice starch increased at relatively lower temperature than native rice starch. Solubility of CLHP rice starch was lower than that of native rice starch. Peak viscosity of CLHP rice starch was lower than that of native starch while holding strength and final viscosity were increased with modification. Breakdown value was lower and setback value was higher than native rice starch. DSC thermal transitions of CLHP rice starch shifted toward lower temperature. Amylopectin-melting enthalpy of CLHP rice starch decreased, whereas it was not affected by the amount of POCl3.

pH-Induced Crosslinking of Rice Starch via Schiff Base Formation

Narudom Srisawang,Saksit Nobsathian,Supa Wirasate,Chayanisa Chitichotpanya 한국고분자학회 2019 Macromolecular Research Vol.27 No.12

Rice starch is an abundant, inexpensive, and biocompatible biopolymer with a wide range of potential applications, particularly in the biomedical field. However, the use of rice starch is limited because of its water-solubility. One way of addressing this is to modify the crosslinking properties. If the degree of crosslinking is sufficiently high, the starch becomes insoluble in water, but it remains swellable. In this study, the first synthesis of pH-induced crosslinking of rice starch without the use of any external chemical crosslinking agent was demonstrated. The crosslinked rice starch was prepared by forming a Schiff base reaction (imine) between the two modified starches: oxidized rice starch (ORS) and amino rice starch (ARS). Here, the ORS and ARS were successfully synthesized, with the content of the glucose units being 32.01 and 27.80 mol%, respectively. Imine bonds were shown to be formed from the aldehyde and amine groups. The relationships between the pH and the degree of crosslinking were also investigated. The chemical structures of the synthesized ORS and ARS and their crosslinked products at different pH levels were determined by 1H NMR, 13C NMR, and FTIR. Furthermore, the effect of pH on swelling behavior was explored. The relationships between the pH and the chemical structure agreed well with the swelling behavior. The swelling ratio was the highest at pH 5 and decreased with increased pH. Under basic conditions, hydrolysis was initiated, reducing the crosslink density and the efficiency of water uptake. The present study suggests that the pH-induced crosslinked rice starch can be a promising biomaterial for controlled release applications.

특수용도 쌀품종 내 전분의 구조적 및 이화학적 특성

이슬(Seul Lee),이은정(Eun-Jung Lee),정현정(Hyun-Jung Chung) 한국식품과학회 2017 한국식품과학회지 Vol.49 No.1

본 연구에서는 국내산 특수용도 쌀품종 내 전분의 분자 및 결정 구조적 특성과 소화 특성을 분석하고 상관성을 살펴봄으로써 향후 쌀가공식품들을 개발하는 기초자료로 활용하고자 하였다. 백진주1호 전분은 다른 쌀품종에 비하여 아밀로스 함량, 아밀로펙틴의 분자량, A 사슬 (DP 6-12), 페이스팅 온도, 치반점도, SDS함량은 유의적으로 낮았으며 아밀로펙틴의 B1 사슬(DP 13-24), 상대적 결정성, 호화온도(T<SUB>o</SUB>, T<SUB>p</SUB>, T<SUB>c</SUB>), 호화 엔탈피(ΔH), 최고점도, 강하점도, RDS 함량이 유의적으로 높은 결과를 나타냈다. 소화율과 구조 및 이화학적 특성 간의 상관성을 분석한 결과 RDS 및 SDS는 아밀로스 함량, 결정성, 호화 온도와 높은 상관성을 보였으며 이는 쌀전분의 구조적 특성과 이화학적 특성의 차이가 전분의 소화율에 영향을 미친 것으로 생각된다. 국내에서 생산된 4가지 특수용도 쌀품종의 전분 분자 및 결정 구조적 특성, 이화학적 특성, 및 소화율 특성이 다르게 나타났으며 이러한 결과는 특수용도 쌀품종을 활용한 가공제품개발에 기초자료로 활용될 것으로 기대된다. Molecular structure, physicochemical properties, and in vitro digestibility of starch from Korean rice cultivars for special uses (Baegjinju 1, Hanareum, Deuraechan, and Goami 4) were investigated. The starch from Baegjinju 1 had the lowest amylose content (9.7%) and Hanareum, Deuraechan, and Goami 4 had intermediate amylose (20-25%) contents. Baegjinju 1 had a lower proportion of short amylopectin branch chains than the other rice starches. Hanareum had the lowest relative crystallinity and the highest intensity ratio of 1047 cm<SUP>−1</SUP>/1022 cm<SUP>−1</SUP> among the rice starches. The starch from Goami 4 had a higher pasting temperature and lower gelatinization enthalpy than the other rice starches. Peak viscosity of rice starch from Baegjinju 1 was substantially higher than peak viscosity of other rice starches. Rice starch from Baegjinju 1 had significantly higher rapidly digestible starch content and lower resistant starch content than other rice starches, whereas there was no significant difference in resistant starch content among the rice starches.

쌀 및 쌀 전분을 활용한 약주의 품질특성

강지은,김재운,최한석,김찬우,여수환,정석태 한국식품저장유통학회 2015 한국식품저장유통학회지 Vol.22 No.3

Yakju, a Korean traditional alcoholic beverage, is made from rice and Nuruk. In this study, the fermentation characteristics of Yakju was investigated using rice and rice starch. Ingredients was classified into raw material (rice, rice starch) and starter (enzyme supplements, modified Nuruk, traditional Nuruk, and yellow rice koji) for fermentation. The crude protein content of rice, rice starch, and starter were determined as follows (%): rice 6.69, rice starch 0.44, enzyme supplements 7.84, modified Nuruk 15.29, traditional Nuruk 14.28, and yellow rice koji 7.28. The alcohol content of rice with traditional Nuruk (20.13±0.12%) was higher than other Yakju. The concentration of organic acids of rice starch-based Yakju (389.83~538.34 mg%) was higher than that of rice Yakju (259.27~357.70 mg%). The concentration of nitrogen compound of rice Yakju (498.38~5976.93 ppm) was higher than that of rice starch-based Yakju (600.43~4463.79 ppm). In line with these findings, further studies will be necessary for the quality analysis of the rice, rice starch and fermented starter (enzyme supplements, modified Nuruk, traditional Nuruk and yellow rice koji).

Modification of Starch Composition Using RNAi Targeting Soluble Starch Synthase I in Japonica Rice

( Hye Jung Lee ),( Moo Geun Jee ),( Joon Ki Kim ),( Franz Mc Nogoy ),( Marjohn C Nino ),( Dal A Yu ),( Me Sun Kim ),( Ming Mao Sun ),( Kwon Kyoo Kang ),( Ill Sup Nou ),( Yong Gu Cho ) 한국육종학회 2014 Plant Breeding and Biotechnology Vol.2 No.3

An increasing preference for good eating quality of rice among consumers has become one of the important considerations in rice breeding. Amylose content is a leading factor affecting eating quality of rice. Amylose composition is determined by the relative activity of soluble starch synthase (SSS) and granule-bound starch synthase (GBSS). This study focused on modifying the expression of SSSI gene which is responsible for amylopectin and amylose synthesis in rice by using RNA interference (RNAi) technology. The transgenic rice plants showed various amylose contents (11-17%) in rice grains. Favorable rice lines were selected according to genomic PCR, transgene expression and amylose contents analysis. A semi-quantitative RT-PCR was carried out to determine the expression level of SSSI gene after flowering of transgenic rice and wild type. Down-regulation of SSSI gene in transgenic plants was evident in the decreasing expression in rice grains. Accordingly, scanning electron microscopy (SEM) analysis revealed uniform size with smooth curves starch granules in down-regulation rice lines, in contrast with the non-uniform granules in wild type. Results indicated that RNAi-SSSI transgenic lines produced low amylose contents that fell between glutinous and non-glutinous rice. This study showed that down-regulation of endogenous SSSI may improve the eating quality in rice.

Identifying genes for resistant starch, slowly digestible starch, and rapidly digestible starch in rice using genome-wide association studies

Ning Zhang,Maike Wang,Ji Fu,Yi Shen,Yi Ding,Dianxing Wu,Xiaoli Shu,Wenjian Song 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background The digestibility of starch is important for the nutritive value of staple food. Although several genes are responsible for resistant starch (RS) and slowly digestible starch (SDS), gaps persist concerning the molecular basis of RS and SDS formation due to the complex genetic mechanisms of starch digestibility. Objectives The objective of this study was to identify new genes for starch digestibility in rice and interprete the genetic mechanisms of RS and SDS by GWAS. Methods Genome-wide association studies were conducted by associating the RS and SDS phenotypes of 104 re-sequenced rice lines to an SNP dataset of 2,288,867 sites using a compressed mixed linear model. Candidate genes were identifed according to the position of the SNPs based on data from the MSU Rice Genome Annotation Project. Results Seven quantitative trait loci (QTLs) were detected to be associated with the RS content, among which the SNP 6 m1765761 was located on Waxy. Starch branching enzymes IIa (BEIIa) close to QTL qRS-I4 was detected and further identifed as a specifc candidate gene for RS in INDICA. Two QTLs were associated with SDS, and the LOC_Os09g09360 encoding lipase was identifed as a causal gene for SDS. Conclusions GWAS is a valid strategy to genetically dissect the formation of starch digestion properties in rice. RS formation in grains is dependent on the rice type; lipid might also contribute to starch digestibility and should be an alternative factor to improve rice starch digestibility.