구기자 분말을 첨가한 데미글라스 소스의 품질특성

구영재,이승주 한국외식산업학회 2023 한국외식산업학회지 Vol.19 No.4

In this study, investigated the physiological activity and quality characteristics for the development of a healthy functional cooking menu by adding Lycii fructus at concentrations of 0, 1, 3, 5, and 7% to demi-glass sauce. The analysis of demi-glace with different amounts of Lycii fructus powder, the more the amount of Lycii fructus powder added, the lower the moisture content and the pH. In addition, the more the amount of Lycii fructus powder added, the higher the total soluble and the viscosity, while the amount of Lycii fructus powder did not affect the salinity. for the color, the more the amount of Lycii fructus powder added, the lower the L, and the higher the a and the b. analysis of the total polyphenol content and DPPH free radical scavenging ability showed that the more the amount of Lycii fructus power added, the higher the antioxidant performance. According to the consumer preference survey, LPDG2 recorded the highest scores in all items except for color, such as appearance, flavor, taste, viscosity, and overall preference. As such, the optimal ratio was found to be 3% when adding Lycii fructus powder to demi-glace. In conclusion, it is essential to continue the research and development of various functional food using Lycii fructus.

생약추출물이 Aspergillus parasiticus Aflatoxin B1 생성에 미치는 영향

정상진 한국약용작물학회 2003 한국약용작물학회지 Vol.11 No.5

본 연구에서 8종의 생약 추출물이 A. parasiticus의 배양시 aflatoxin B1 생성에 미치는 효과를 조사하였다. 배지의 pH는 배양 3일 후에 모든 생약 추출물이 pH 4 이하를 나타냈으며 구기자, 오매, 계피, 두충은 배양 6일에 다시 pH 4 이상으로 상승하였고 이중 대추가 배양 기간 중 가장 낮은 pH를 나타냈다. 균체 생성량은 모든 실험군이 대조군보다 높았으며 갈근, 두충, 오미자, 대추, 오매,구기자, 목과의 순으로 나타났다. 이중 갈근이 최대 생성량을 나타냈으며 목과가 가장 낮은 생성량을 나타냈다. Aflatoxin B1은 갈근과 대추 추출물을 제외한 모든 실험군에서 생성이 억제되었다. 특히 계피, 오매, 두충 구기자, 오미자 추출물 에서 aflatoxin B1 생성이 현저히 저하되었으며 계피가 가장 큰 억제 효과를 나타냈다. 균체량이 많이 생성되면 aflatoxin B1 생성이 적어지고 균체량이 적게 생성되면 aflatoxin B1의 생성이 많아졌다. Aspergillus parasiticus에 의해 aflatoxin B1을 가장 적게 생성하는 계피와 가장 많이 생성하는 갈근 추출물의 총단백질 생성량은 계피는 3일째 (34.5%), 갈근 ext ract는 4일째 (36.4%) 총단백질의 함량이 최대가 되어 균체 내의 총단백질의 함량은 계피와 갈근 추출물을 첨가한 시험군이 게조군의 총단백질 함량 (32.7%)보다 약간 많았으며 aflatoxin B1 생성 및 축적이 최대가 되는 시기는 총단백질량이 최대가 되는 시기보다 대체로 1일 정도 늦게 나타났다. The influences of the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix, Lycii Fructus, Zizyphi Fructus, Schisandrae Fructus, Mume Fructus, Chaenomelis Fructus on mycelial growth and aflatoxin B1 production from Aspergillus parasiticus were analyzed. The pH of the culture media were reduced to below pH 4 by all the herbal extracts after 3 days incubation. However, the pH of the culture media increased above pH 6 after 6 days incubation using the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix and Lycii Fructus. The mycelial growth of A. parasiticus was increased over the amount of the control. Puerariae Radix produced the largest amount of mycelial growth and Chaenomelis Fructus produced the smallest amount of mycelial growth. The productions of aflatoxin B1 from A. parasticus culture were increased by the extracts of Puerariae Radix and Zizyphi Fructus, while inhibited by the extracts of Cinnamomi Cortex, Eucommiae Cortex, Lycii Fructus, Schisandrae Fructus, Mume Fructus and Chaenomelis Fructus. In particular, the extracts of Cinnamomi Cortex, Lycii Fructus and Schisandrae Fructus almost inhibited the production of aflatoxin B1. The production of the total protein from Cinnamomi Cortex, which produced much less aflatoxin B1, and Puerariae Radix, which produced a great deal of aflatoxin B1 from A parasticus were slightly higher than the production of the total protein of the control medium.

생약추출물이 Aspergillus parasiticus의 Aflatoxin B₁ 생성에 미치는 영향

정상진(Jeong Sang Jin) 한국약용작물학회 2003 한국약용작물학회지 Vol.11 No.5

The influences of the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix, Lycii Fructus, Zizyphi Fructus, Schisandrae Fructus, Mume Fructus, Chaenomelis Fructus on mycelial growth and aflatoxin B₁ production from Aspergillus parasiticus were analyzed. The pH of the culture media were reduced to below pH 4 by all the herbal extracts after 3 days incubation However, the pH of the culture media increased above pH 6 after 6 days incubation using the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix and Lycii Fructus. The mycelial growth of A parasiticus was increased over the amount of the control, Puerariae Radix produced the largest amount of mycelial growth and Chaenomelis Fructus produced the smallest amount of mycelial growth. The productions of aflatoxin B₁ from A parasticus culture were increased by the extracts of Puerariae Radix and Zizyphi Fructus, while inhibited by the extracts of Cinnamomi Cortex, Eucommiae Cortex, Lycii Fructus , Schisandrae Fructus, Mume Fructus and Chaenomelis Fructus. In particular, the extracts of Cinnamomi Cortex, Lycii Fructus and Schisandrae Fructus almost inhibited the production of aflatoxin B₁. The production of the total protein from Cinnamomi Cortex, which produced much less Stoxin B₁, and Puerariae Radix, which produced a great deal of aflatoxin B₁ from A parasticus were slightly higher than the production of the total protein of the control medium.

생약추출물이 Aspergillus parasiticus의 Aflatoxin B1 생성에 미치는 영향

정산진 한국약용작물학회 2003 韓國藥用作物學會誌 Vol.11 No.5

The influences of the extracts from Cinnamomi cortex, Eucommiae Cortex, Puerariae Radix, Lycii Fructus, Zixyphi Fructus, Schisandrae Fructus, Mume Fructus, Chaenomelis Fructus on mycelial growth and aflatoxin B1 production from Aspergillus parasiticus were analyzed. The pH of the culture media were redeced to below pH 4 by all the herbal extracts after 3 days incubation. However, the pH of the culture media increased above pH 6 after 6 days incubation using the extracts from Cinnamomi Cortex, Eucommiae Cortex, Puerariae Radix and Lycii Fructus. The mycelial growth of A. parasiticus was increased over the amount of the control. Puerariae Radix produced the largest amount of mycelial growth and Chaenomelis Fructus produced the smallest amount of mycelialgrowth. The productions of aflatoxin B1 from A. parasticus culture were increased by the extracts of Puerariae Radix and Zizyphi Fructus, while inhibited by the extractso of Cinnamomi Cortes, Eucommiae Cortex, Lycii Fructus, Schisandrae fructus, Mume Fructus and Chaenomelis Fructus. In particular, the extracts of Cinnamomi Cortex, Lycii Fructus and schisandrae Fructus almost inhibited the preduction of aflatoxin b1. The production of the total protein from Cinnamomi Cortex, which produced much less aflatoxin B1, and Puerariae Radix, whichproduced a great deal of aflatoxin B1 from A. parasticus were slightly higher than the production of the total protein of the control medium.

Determination of Betaine in Fructus Lycii Using Hydrophilic Interaction Liquid Chromatography with Evaporative Light Scattering Detection

Hyun Du Shin,서준혁,Junghyun Kim,Hyeyeon Lee,엄한영,김운용,양동혁,한상범,염정록 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

A simple new method was developed for the determination of betaine in Fructus Lycii using hydrophilic interaction liquid chromatography with evaporative light scattering detection (HILIC-ELSD). Good chromatographic separation and reasonable betaine retention was achieved on a Kinetex HILIC column (2.1 × 100 mm,2.6 μm) packed with fused-core particle. The mobile phase consisted of (A) acetonitrile and (B) 10 mM ammonium formate (pH 3.0)/acetonitrile (90/10, v/v). It was used with gradient elution at a flow rate of 0.7 mL/min. The column temperature was set at 27.5 °C and the injection volume was 10 μL. The ELSD drift tube temperature was 50 °C and the nebulizing gas (nitrogen) pressure was 3.0 bar. Stachydrine, a zwitterionic compound, was used as an internal standard. Calibration curve over 10-250 μg/mL showed good linearity (R2> 0.9992) and betaine in the 70% methanol extract of Fructus Lycii was well separated from other peaks. Intraand inter-day precision ranged from 1.1 to 3.0% and from 2.4 to 5.3%, respectively, while intra- and inter-day accuracy ranged from 100.0 to 107.0% and from 94.3 to 103.9%, respectively. The limit of quantification (LOQ) was 10 μg/mL and the recoveries were in the range of 98.2-102.7%. The developed HILIC-ELSD method was successfully applied to quantitatively determine the amount of betaine in fourteen Fructus Lycii samples from different locations, demonstrating that this method is simple, rapid, and suitable for the quality control of Fructus Lycii.

Determination of Betaine in Fructus Lycii Using Hydrophilic Interaction Liquid Chromatography with Evaporative Light Scattering Detection

Shin, Hyun-Du,Suh, Joon-Hyuk,Kim, Jung-Hyun,Lee, Hye-Yeon,Eom, Han-Young,Kim, Un-Yong,Yang, Dong-Hyug,Han, Sang-Beom,Youm, Jeong-Rok Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2

A simple new method was developed for the determination of betaine in Fructus Lycii using hydrophilic interaction liquid chromatography with evaporative light scattering detection (HILIC-ELSD). Good chromatographic separation and reasonable betaine retention was achieved on a Kinetex HILIC column ($2.1{\times}100mm$, $2.6{\mu}m$) packed with fused-core particle. The mobile phase consisted of (A) acetonitrile and (B) 10 mM ammonium formate (pH 3.0)/acetonitrile (90/10, v/v). It was used with gradient elution at a flow rate of 0.7 mL/min. The column temperature was set at $27.5^{\circ}C$ and the injection volume was $10{\mu}L$. The ELSD drift tube temperature was $50^{\circ}C$ and the nebulizing gas (nitrogen) pressure was 3.0 bar. Stachydrine, a zwitterionic compound, was used as an internal standard. Calibration curve over $10-250{\mu}g/mL$ showed good linearity ($R^2$ > 0.9992) and betaine in the 70% methanol extract of Fructus Lycii was well separated from other peaks. Intraand inter-day precision ranged from 1.1 to 3.0% and from 2.4 to 5.3%, respectively, while intra- and inter-day accuracy ranged from 100.0 to 107.0% and from 94.3 to 103.9%, respectively. The limit of quantification (LOQ) was $10{\mu}g/mL$ and the recoveries were in the range of 98.2-102.7%. The developed HILIC-ELSD method was successfully applied to quantitatively determine the amount of betaine in fourteen Fructus Lycii samples from different locations, demonstrating that this method is simple, rapid, and suitable for the quality control of Fructus Lycii.

RESEARCH ARTICLE : Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD

( Bing Tian Zhao ),( Su Yang Jeong ),( Kyoung Hwangbo ),( Dong Cheul Moon ),( Eun Kyoung Seo ),( Dongho Lee ),( Je Hyun Lee ),( Byung Sun Min ),( Eun Sook Ma ),( Jong Keun Son ),( Mi Hee Woo ) 영남대학교 약품개발연구소 2014 영남대학교 약품개발연구소 연구업적집 Vol.24 No.0

A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 A) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.

구기자의 혈당강하작용

신준수,김경순,정기화,정춘식,고광호,박정일,허훈,김박광,Shin, Joon-Su,Kim, Kyoung-Soon,Jeong, Gi-Hwa,Cheong, Chun-Sik,Ko, Kwang-Ho,Park, Jeong-Hill,Huh, Hoon,Kim, Bak-Kwang 한국생약학회 1997 생약학회지 Vol.28 No.3

To elucidate antidiabetic activity of Lycii Fructus. The extract of the Lycii Fructus was subjected to bioactivity-guided fractionation. The extract of the Lycii Fructus was partitioned suceesively with hexane, dichloromethane, ethylacetate and butanol. The butanol fraction, which is most abundant in the Lycii Fructus was subjected to silica gel column chromatography and resulted in seven fractions namely Fr. I - Fr. VII. Among tested several subfractions, Fr IV. Showed the highest antidiabetic activity in the streptozotocin-induced model rat.

황칠나무, 산수유, 구기자 복합 초임계유체추출물의 항산화 및 항노화 효과

신동철 ( Dong Chul Shin ),김귀철 ( Gwui Cheol Kim ),송시영 ( Si Young Song ),김희진 ( Hee Jin Kim ),양재찬 ( Jae Chan Yang ),김보애 ( Bo Ae Kim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6

Objectives: The purpose of this study was to investigate antiaging and antioxidant effects on cultured human skin fibroblast with supercritical fluid extracts of Dendropanax morbifera, Corni fructus and Lycii Fructus. Methods : Supercritical fluid extraction (SFE) technique was applied to extract from three medicinal plants including stem of Dendropanax morbifera, Corni fructus and Lycii Fructus. Antioxidant activity of extract was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. These extracts were tested for cell viability on HS68 skin fibroblast by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. We investigated the effects of Ultraviolet-B irradiation on cytotoxicity, type 1 collagen, elastin level and oxidative damage in cultured human skin fibroblast (HS68). Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. Results : The extracts obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The supercritical fluid extracts of complex herbal medicine showed low cytotoxicity as more than 100% cell viability in 100ppm/ml concentration. HS68 fibroblasts were survived 70% at 120 mJ/cm2 UVB irradiation and treated tumor necrosis factor (TNF)-alpha. The levels of aging factors and cytotoxicity were decreased by supercritical fluid extract of complex herbal medicine. Conclusions : These results suggest that supercritical fluid extracts may have value as the potential antioxidant and antiaging medicinal plant.

한약재복합 추출물의 인간피부섬유아세포 HS68에 대한 항노화 효과

신동철 ( Dong Chul Shin ),김귀철 ( Gwui Cheol Kim ),송시영 ( Si Young Song ),김희진 ( Hee Jin Kim ),양재찬 ( Jae Chan Yang ),이용화 ( Yong Hwa Lee ),김보애 ( Bo Ae Kim ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.2

Objectives : The purpose of this study was to investigate antiaging and antioxidant effects on cultured human skin fibroblast with 80% ethanol extracts of plants including of stem of Dendropanax morbifera, Corni fructus and Lycii Fructus. Methods : An ethanol extract of three medicinal plants including stem of Dendropanax morbifera, Corni fructus and Lycii Fructus. Extracts were assessed to determine the mechanism of antioxidant and antiaging activities. Antioxidant activity of extract was evaluated by two different assays as 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and super oxide dismutase (SOD) like activities. These extracts were tested for cell viability on HS68 skin fibroblast by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. We investigated the effects of Ultraviolet-B irradiation on cytotoxicity, type 1 collagen, elastin level and oxidative damage in cultured human skin fibroblast (HS68). Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. Results : The extracts obtained dose-dependently increased the scavenging activity on DPPH radical scavenging activity and SOD like activity. The extracts of complex herbal medicine showed low cytotoxicity as more than 100% cell viability in 100ppm/ml concentration. HS68 fibroblasts were survived 70% at 120 mJ/cm2 UVB irradiation and treated tumor necrosis factor (TNF)-alpha. The levels of aging factors and cytotoxicity were decreased by ethanol extract of complex herbal medicine. Conclusions : These results suggest that ethanol extracts of complex medicinal plants of including of stem of Dendropanax morbifera, Corni fructus and Lycii Fructus may have value as the potential antioxidant and antiaging medicinal plant.