Effect of Irrigation Frequency on Growth and Functional Ingredient Contents of Gynura procumbens Cultivated in Hydroponics System

Kyu-Hoi Lee,Sung-Hee Lee,Eun-Sol Yeon,Who-Bong Chang,Ju-Hyoung Kim,Jae-Ho Park,Tong-Min Sa,Gwang-Hyun Han,Jin-Hee Park,Sook-Jin Kim 한국토양비료학회 2020 한국토양비료학회지 Vol.53 No.2

Gynura procumbens (Family Asteraceae) is a medicinal plant commonly found in tropical Asian countries such as Malaysia, Indonesia, Thailand, China, and Vietnam. It is widely used as a traditional medicine in many different countries for the treatment of a variety of health ailments such as kidney discomfort, hypertension, constipation, diabetes mellitus, and rheumatism. The objective of this study was to investigate the effect of irrigation on growth and functional ingredients of Gynura procumbens. Gynura procumbens were planted in a greenhouse on April 24 (2019), and irrigation was carried out throughout the entire growth period at 12, 24, 48, 72hour cycles using drip hydroponics system. The total height of Gynura procumbens became higher with shorter irrigation cycle, whereas a smaller height was recorded with the longer periods. The maximum yield was recorded for 12 - 24 h irrigation cycle, but it tended to decrease as the irrigation cycle became longer. The leaf marketability based on the irrigation cycle, leaf length, leaf width, leaf weight, and leaf thickness were best for the 24 h irrigation cycle treatment. The total polyphenols was higher for the 72 h irrigation cycle treatments compared to the others. Vitamin C concentrations were high at 48 and 72 h irrigation cycle treatment. Hence, the growth of Gynura procumbens based on irrigation cycle was maximum at 24-hour, and total polyphenol and vitamin C content was highest at 72-hour irrigation cycle treatment.

Effect of Shading on Growth and Functional Ingredient Contents of Gynura procumbens Cultivated in Hydroponics System

Kyu-Hoi Lee,Sung-Hee Lee,Eun-Sol Yeon,Who-Bong Chang,Ju-Hyoung Kim,Jae-Ho Park,Tong-Min Sa,Gwang-Hyun Han,Jin-Hee Park,Sook-Jin Kim 한국토양비료학회 2020 한국토양비료학회지 Vol.53 No.2

Gynura procumbens (Family Asteraceae) is a medicinal plant commonly found in tropical Asian countries such as China, Thailand, Indonesia, Malaysia, and Vietnam. It is widely used as a traditional medicine in many different countries for the treatment of a variety of health ailments such as kidney discomfort, rheumatism, diabetes mellitus, constipation, and hypertension. The objective of this study was to investigate the effect of shading on growth and functional ingredients of Gynura procumbens. Gynura procumbens was planted in a greenhouse on April 24 (2019), and shading treatments were carried out throughout the entire growth period at 0%, 35%, 55%, and 75% using a black net. The total plant height of Gynura procumbens was highest for the 75% shading treated ones. Additionally, the stem diameter and yield was higher for all the shading treated plants compared to the non-shaded control plants. The DPPH radical scavenging activity of the 0% and 35% shading treated plants were significantly higher than the plants exposed to 55% and 75% shading treatment at 0.5% and 2.5% sample concentrations. The total flavonoids and vitamin C concentrations had also shown the same trend, whereas rutin content was highest for 0% shading treatment and the lowest was for 55% and 75% shading treatments. Hence, this study suggests that 35% shading treatment on Gynura procumbens can be considered as an advantageous condition for their growth and functional ingredients.

유기 수경재배 시 천연배지 조성에 의한 명월초 생육 및 항산화 활성

이성희,김주형,박재호,이규회 한국유기농업학회 2019 韓國有機農業學會誌 Vol.27 No.3

Gynura procumbens (Family Asteraceae) is a medicinal plant commonly found in tropical Asia countries such as China, Thailand, Indonesia, Malaysia, and Vietnam. Traditionally, it is widely used in many different countries for the treatment of a wide variety of health ailments such as kidney discomfort, rheumatism, diabetes mellitus, constipation, and hypertension. The objective of this study was to select the natural growth media for hydroponic cultivation of Gynura procumbens. The commercially available thirty four different organic nutrient solutions were analyzed for growing Gynura procumbens. After analysis, two organic nutrient solution were selected, mixed (pH 5.5~6.5/EC 0.5~1.0 ds/m) and then used as organic fertilizers. Thirty day old seedlings were planted and the environmental conditions of the rhizosphere were also examined to select the natural media composition. The minimum temperature of rhizosphere by type of natural media was highest in cocopeat media and lowest in perlite media. Furthermore, plant growth was examined from 50 days old seedlings, and vermiculite was observed to be most effective and perlite was the least effective component as natural media. The total polyphenol content of the studied plant material was also higher in vermiculite in comparison to other growth media. The Gynura procumbens yields were also higher in vermiculite in comparison to other growth media. Thus, vermiculite can be used to improve Gynura procumbens plant growth as a natural growth media.

Inhibitory Effects of an Aqueous Extract of Gynura procumbens on Human Mesangial Cell Proliferation

Hee Jae Lee,Byung-Cheol Lee,Joo-Ho Chung,Sumali Wiryowidagdo,Wanjoo Chun,Sung-Soo Kim,Hyunsook Kim,Myeon Choe 대한생리학회-대한약리학회 2007 The Korean Journal of Physiology & Pharmacology Vol.20 No.3

<I>Gynura procumbens</I> (Lour.) Merr. has been used in some parts of Southeast Asia as a folk medicine to treat kidney diseases, diabetes mellitus, and hyperlipidemia. The present work was undertaken to prove the mechanisms of <I>G. procumbens</I> in the management of glomerular diseases. We investigated the effect of an aqueous extract of G. procumbens on cell proliferation, DNA synthesis, and the expressions of TGF-β1, <I>PDGF-BB, CDK1, CDK2, </I>and CDK4 in fetal bovine serum-activated human mesangial cells (MCs). The G. procumbens extract inhibited proliferation, DNA synthesis, expressions of <I>PDGF-BB, CDK1,</I> and <I>CDK2 </I>mRNA, and expression of TGF-β1 protein in MCs. The inhibitory effect of G. procumbens on MC proliferation may be mediated by suppression of PDGF-BB and TGF-β1 expressions and the modulation of <I>CDK1 </I>and <I>CDK2</I> expression. Therefore, <I>G. procumbens </I>shows promise as an adjunct therapy in preventing progressive renal diseases.

명월초에서 분리한 Broad bean wilt virus 2 분리주들의 염기서열 분석과 기주반응 비교

최성안,박태선,박지수,민동주,홍진성 강원대학교 농업생명과학연구원 2019 강원 농업생명환경연구 Vol.31 No.3

Gynura procumbens (Myeongwolcho or longevity spinach) showing virus-like mosaic and yellowing symptoms was collected in Chuncheon. To identify the virus from G. procumbens, RT-PCR analysis was performed using several genus-specific primers sets. BBWV2-Gyp was identified from the amplified target product using Fabavirus genus-specific primers. BBWV2- Gyp had a similar host range pattern to BBWV2–SN that has already reported in G. procumbens. BBWV2-Gyp induced systemic mosaic and BBWV2-SN induced systemic necrotic spots on Phaseolus angularis. To investigate the sequence specificity, BBWV2-Gyp RNA2 was sequenced and compared with other BBWV2 isolates. Sequence homologies and phylogenetic analyses clearly indicated that BBWV2-Gyp had the closest relationship to BBWV2-SN. Also, BBWV2- Gyp had 98.5%, 98.8% and 98.5% homologies with movement protein (MP), large coat protein (LCP) and small coat protein (SCP) segments, respectively, of BBWV2-SN at amino acid level. Although two BBWV2 isolates from G. procumbens have high nucleotide sequence similarity (99%), they have distinct biological properties.

High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens

지은이,Nornita Sham Atong,안우석,안명석,민병환,Saleh Kadzimin,김석원 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.1

The efficient plant regeneration system from embryogenic cell suspension cultures of Gynura procumbens (Lour.) Merr. is described. Leaf, stem and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) in various concentrations (0, 0.1, 0.3, 1.0 and 3.0 mg l−1). Leaf, stem and petiole explants formed pale-yellow nodular callus and off-white calluses at a frequency of 100% when cultured on MS medium supplemented with more than 1 mg l−1 of 2,4-D after 4 weeks incubation. However, only 20% of pale-yellow nodular callus derived from petiole explants developed into white embryonic structures. Upon transfer to MS basal medium without growth regulators, these white embryonic structures differentiated into somatic embryos. Embryogenic cell suspension cultures were initiated from petiole-derived pale-yellow nodular calluses. More than 73.2% of regenerated plantlets via somatic embryogenesis produced roots on MS medium supplemented with 0.1 mg l−1 α-naphthaleneacetic acid and 1 mg l−1 indole-3-butyric acid (IBA), respectively. Rooted plantlets were successfully transplanted to soil mixture of sterile vermiculite and potting soil (1:1) and grown to maturity in a growth chamber, achieving a survival rate of > 95%. The plant regeneration system from embryogenic cell suspension cultures of G. procumbens established in this study could be applied as an alternative for mass proliferation as well as molecular breeding for quality improvement of G. procumbens.

명월초에서 분리한 오이모자이크바이러스의 감염 첫 보고

권준,홍진성 한국식물병리학회 2017 식물병연구 Vol.23 No.4

In March 2016, an isolate of Cucumber mosaic virus (named Gyp-CMV) was isolated from the Sambungai (Gynura procumbens) showing the symptoms of mosaic and chlorosis. The isolate Gyp-CMV was characterized by disease reactions in several indicator plants, reverse transcription-polymerase chain reaction (RT-PCR), PCR-restriction fragment length polymorphism, and sequence analysis of movement protein (3a) and coat protein (CP) genes. Tobacco, tomato, pepper, ground cherry, and lambsquarters (Chenopodium quinoa and C. amaranticolor) appeared typical CMV symptoms, but zucchini and cucumber were not infected. Phylogenetic analysis of the 3a and CP gene indicated that Gyp-CMV belongs to the CMV subgroup II. Sequence identities of the Gyp-CMV 3a and CP genes showed 99.3% and 100% to that of Hnt-CMV at amino acid level. To our knowledge, this is the first report of CMV infection in Gynura procumbens.

Sterols and Sterol Glycosides from the Leaves of Gynura procumbens

Sadikun, A.,Aminah, I.,Ismail, N.,Ibrahim, P. The Korean Society of Pharmacognosy 1996 Natural Product Sciences Vol.2 No.1

A mixture of sterols containing ${\beta}-sitosterol$ and stigmasterol (1), and sterol glycosides containing $3-O-{\beta}-D-glucopyranosyl$ ${\beta}-sitosterol$ and $3-O-{\beta}-D-glucopyranosyl$ stigmasterol (2) were isolated from the leaves of Gynura procumbens. After acetylation of 2 with pyridine-acetic anhydride, $3-0-{\beta}-D-tetra-O-acetylglucopyranosyl$ ${\beta}>-sitosterol$ (3) was isolated.

Effects of Extracts and Fractions of Gynura procumbens on Rat Atrial Contraction

Omar Saad Saleh Abrika,Mun Fei Yam,Mohd. Zaini Asmawi,Amirin Sadikun,Hamady Dieng,Elssanousi Ali Hussain 사단법인약침학회 2013 Journal of Acupuncture & Meridian Studies Vol.6 No.4

There is currently a great deal of research interest in utilizing plant compounds against humandiseases, including hypertension. The present study investigated the effects of differentextracts and fractions from leaves of Gynura procumbens Merr. on rat atrial contractionin vitro. Isolated left and right atria, mounted in a 20-ml organ bath, were allowed to equilibratefor 15 min before the application of the extracts or fractions. The extracts (petroleum-ether extract (PE) and methanol extract (ME)) and the fractions (chloroform fraction(CHL), ethyl-acetate fraction (EA), n-butanol fraction (NB) and water fraction (WA) ofthe methanol extract) were tested at three concentrations (0.25, 0.5 and 1.0 mg/ml), witha b-adrenergic agonist (isoprenaline) as a control. All data on contraction responses werelog-transformed and analyzed. When exposed to the different extracts, both atria tendedto exhibit greater contractive responses with the NB whereas cardiac contractions hada tendency to be reduced with most other extracts. For a given extract, the contraction responseswere particularly greater at 0.5 mg/ml for the right atrium and at 1 mg/ml for theleft atrium. Further analysis focusing on the NB fraction revealed that positive inotropismwas greater in left atria exposed to highly-concentrated F2 and F3 sub-fractions. Taken together,our results suggest that NB extracts and fractions from the G. procumbens-leafmethanol extract have positive inotropic activities and, hence, can be considered as an alternative/traditional medicine against increased blood pressure in humans or can be usedin strategies aimed at finding antihypertensive biomolecules from an accessible source.

명월초 추출물의 항산화성분 분리 및 루틴의 정량분석

전형주(Jeon, Hyeong-Ju),차윤환(Cha, Yun-Hwan),장혜륜(Jang, Hye-Rroon) 한국웰니스학회 2016 한국웰니스학회지 Vol.11 No.4

본 연구는 명월초 추출물의 항산화성분 분리 및 루틴의 정량분석을 평가하기 위한 것이다. 항산화성분의 함량을 가장 많이 함유한 명월초 잎 에탄올추출물(Leaf-E)에서 기능성 소재로서의 연구를 수행하였다. Flavonol 배당체의 분리 및 동정과 LDL-antioxidant 활성분석결과 80% MeOH 수용액을 여과, 감압 농축하여 MeOH 추출물을 얻었다. 이를 EtOAc 분획, n-BuOH 분획, H2O 분획으로 나누었으며, EtOAc 분획에 대해 silica gel column chromatography를 실시하여 4종의 Flavonol 배당체를 분리 및 정제하였다. 1H-NMR, 13C-NMR, DEPT spectrum 및 Mass spectrum 등을 통하여 화합물 1-Astrgalin, 화합물 2-Isoquercitin, 화합물 3-Kaempferol, 화합물 4-Rutin의 구조를 결정하였고, Rutin 함량 분석을 통해 명월초의 기능성 소재 및 융합소재로의 가능성을 평가하였다. This study is to evaluate the antioxidant activity and functionality of the Gynura Procumbens. The content of most commonly contains the content of the antioxidant patients early leaf ethanol was studied as a functional material in the extract (Leaf-E). Flavonol glycosides and Identification and separation of the LDL-antioxidant extracted by the activity analysis of 80% MeOH aqueous solution was filtered and concentrated under reduced pressure to obtain a MeOH extract. In EtOAc fraction, n-BuOH fraction, fractions were divided into H2O, the four kinds of Flavonol glycosides isolated and purified by silica gel column chromatography performed for the EtOAc fraction. 1H-NMR, 13C-NMR, DEPT spectrum and Mass spectrum, such as through the 1-Astrgalin compound, the compound 2-Isoquercitin, compound 3-kaempferol, to determine the structure of the compound 4-Rutin. Rutin substance containing a name beginning with a variety of activities that will be enough the value of a functional food material and fused material.