저온 상압플라즈마에 의한 Hairless Mouse-2 마우스 조직의 Candida albicans 사멸 효과

박상례 ( Sang Rye Park ),김규천 ( Gyoo Cheon Kim ) 한국치위생과학회 2014 치위생과학회지 Vol.14 No.1

본 연구는 저온 상압 플라즈마 장치를 이용하여 구강점막 질환을 일으키는 C. albicans 균을 효과적으로 사멸하기 위해 시행하였다. 조직에 적합하게 처리될 수 있도록 저온 상압 플라즈마 장치를 고안하고, 먼저 agar plate에 C. albicans 균을 처리하여 플라즈마를 조사한 결과 agar plate 표면에 C. albicans 균을 처리 후 저온 상압 플라즈마 장치를 적용한 결과 60초 처리시 1.2 cm, 180초 처리시 1.4 cm, 300초 처리시 1.7 cm의 박테리아 생장 억제 구간이 나타나는 것을 확인하였다. 또한, 조직에서의 구강병원균 사멸 효과를 확인하기 위해 HRM-2 마우스 조직에 C. albicans 균을 처리하여 저온 상압 플라즈마를 조사 시 마우스 조직 표면에 C. albicans 균을 오염시켜 저온 상압 플라즈마 처리후 CFU 방법으로 측정한 결과 300초간 1회 처리시 2 log CFU/ml, 300초간 2회 처리시 3 log CFU/ml, 300초간 3 회처리시 6 log CFU/ml의 균 수 감소 효과가 나타나는 것을 확인하였다(p<0.05). 따라서, 저온의 저온 상압 플라즈마 장치는 효과적으로 구강 병원균을 사멸시킬 수 있으며, 구강점막질환 치료 장비로서 사용될 수 있을 것으로 생각된다. The purpose of this study was to investigate the killing effect of Candida albicans on hairless mouse-2 (HRM-2) mouse tissues. We tested theeffectiveness of a non-thermal atmospheric pressure plasma in killing C. albicans strains. The viability of C. albicans was determined by countingthe colony forming units (CFU), after non-thermal atmospheric pressure plasma treatment. When non-thermal atmospheric pressure plasma wasrepeatedly treated on mouse skin which inoculated with C. albicans. The C. albicans cells were planted on skin tissue, and then the infected mousetissue was exposed to non-thermal atmospheric pressure plasma for 0 sec, 60 sec, 180 sec and 300 sec. The death rate of C. albicans wasincreased in dependent with treatment times. The three times of non-thermal atmospheric pressure plasma at the interval of 10 minutes significantlyshowed the 6 log CFU/ml reduction of death rate on HRM-2 mouse tissues. Thus, non-thermal atmospheric pressure plasma could be used forthe disinfection of C. albicans on oral surface.

Non-Thermal Atmospheric-Pressure Plasma Possible Application in Wound Healing

Haertel, Beate,von Woedtke, Thomas,Weltmann, Klaus-Dieter,Lindequist, Ulrike The Korean Society of Applied Pharmacology 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.6

Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

Hairless 마우스 조직에서의 저온 상압 플라즈마의 안정성 평가

박상례 ( Sang Rye Park ),김규천 ( Gyoo Cheon Kim ),최별보라 ( Byul Bora Choi ),김지영 ( Ji Young Kim ) 대한예방치과·구강보건학회 2014 大韓口腔保健學會誌 Vol.38 No.3

Objectives: The aim of the present study was to evaluate the stability of non-thermal atmosphericpressure plasma on Candida albicans in hairless mouse-2 (HRM-2) tissues. Methods: HRM-2 mice were subjected to non-thermal atmospheric-pressure plasma jet treatment using an optical fiber probe and monitored using a thermometer. The skin of HRM-2 mice was treated with plasma jet for 0, 60, 180, and 300 s per day for 5 days. After plasma treatment, morphological changes in Candida albicans on the skin of these mice were examined using a scanning electron microscope. Biopsy of the plasma-treated skin was performed and the tissues were histologically analyzed using hematoxylin and eosin (H&E) and Masson’s trichrome stains. Results: The scanning electron microscopic images revealed the morphological changes in the membrane structure of the plasma-treated Candida albicans. Histological analysis showed that non-thermal plasma treatment did not cause epidermal damage or tissue inflammation and did not significantly modify the collagen layers of the mouse skin. Conclusions: The results of this study suggest that non-thermal atmospheric-pressure plasma might be safe and effective for clinical applications in the field of dentistry.

Spectroscopic investigation of atmospheric pressure cold plasma jet produced in dielectric barrier discharge

Imran Mubashair,Khan Majid,Javed M.A.,Ahmad S.,Qayyum A. 한국물리학회 2023 Current Applied Physics Vol.50 No.-

Non-thermal cold atmospheric pressure plasma jet (APPJ) produced in multi-pulse dielectric barrier discharge (DBD) preferentially heats the electrons that subsequently transfer their energy to the other plasma species converting them into reactive species. Therefore the characterization of different energy groups of electrons is essential for efficient production and control of reactive plasma species contributing to surface modifications. Here we present the spectroscopic investigations of the APPJ produced in multi-pulse DBD using pin electrode configuration. Boltzmann plot relates the emission intensities of the several Ar–I spectral lines to their corresponding threshold excitation energies to give the electron temperature more precisely. The Stark broadening of the Ar–I (696.54 nm) line profile gives the electron number density after de-convoluting the contributions of the Doppler and instrumental broadenings. Plasma measurements correlate the gas flow rate, applied jet power across the electrodes, and a group of electrons contributing to optical emission. Sequential imaging correlates the size of the plasma bullets momentarily with the discharge pulse for different argon flow rates and applied jet powers. With an increasing flow rate of up to 1.4 L/min, the plasma plume of the jet becomes more elongated, intense, and spatially uniform. However, the glow intensity and size of the plume start reducing with a further increase in gas flow rate. Experimental findings propose plasma processing for small-scale localized plasma surface treatments.

대기압 유전체장벽방전 플라즈마에 의한 식품유해 미생물 살균

이승제,송윤석,박유리,류승민,전형원,엄상흠 한국식품위생안전성학회 2017 한국식품위생안전성학회지 Vol.32 No.3

This study aimed to explore the potential for food-industry application of atmospheric pressure dielectric barrier discharge plasma (atmospheric pressure DBD plasma) as a non-thermal sterilization technology for microorganism. The effects of the key parameters such as power, oxygen ratio, exposure time and distance on Escherichia coli KCCM 21052 sterilization by the atmospheric pressure DBD plasma treatment were investigated. The experimental results revealed that increasing the power, exposure time or oxygen ratio and decreasing the exposure distance led to an improvement in the sterilization efficiency of E. coli. Furthermore, the atmospheric pressure DBD plasma (1.0 kW power, 1.0% (v/v) O2, 5 min exposure time and 20 mm exposure distance) treatment was very effective for the sterilization of food-borne pathogenic bacteria. The sterilization rate of E. coli, Bacillus cereus KCCM 40935, Bacillus subtilis KCCM 12027, Bacillus thuringiensis KCCM 11429 and Bacillus atrophaeus KCCM 11314 were 72.3%, 74.6%, 88.5%, 84.7% and 91.3%, respectively.

Electron Temperature and Density of Non-Thermal Atmospheric Pressure Argon Plasma Jet by Convective Wave Packet Model

Jirapong Sornsakdanuphap,Pradoong Suanpoot,홍영준,Bhagirath Ghimire,조광섭,엄환섭,김도영,김윤지,최은하 한국물리학회 2017 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.70 No.11

The propagation velocities (ug) of argon plasma jet are obtained by intensified charge coupled device (ICCD) camera images at fixed gate width time of 5 ns. The propagation velocities in upstream and downstream regions are in the order of 104 - 105 m/s. The plasma ambipolar diffusion velocities are measured to be in the order of 10 - 102 m/s. Plasma jet discharges are generated by sinusoidal power supply in varying voltages from 2 to 4 kV at repetition frequency of about 40 kHz. By employing convective wave packet model, the electron temperature (Te) inside plasma bullet for argon non-thermal atmospheric pressure plasma jet is estimated to be about 1.18 eV. Also, the electron density (ne) is found to be 8.0 × 1014 - 2.5 × 1015 cm−3.

Sterilization effect of atmospheric pressure non-thermal air plasma on dental instruments

Su-Jin Sung,Jung-Bo Huh,Mi-Jung Yun,Brian Myung W. Chang,Chang-Mo Jeong,Young-Chan Jeon 대한치과보철학회 2013 The Journal of Advanced Prosthodontics Vol.5 No.1

PURPOSE. Autoclaves and UV sterilizers have been commonly used to prevent cross-infections between dental patients and dental instruments or materials contaminated by saliva and blood. To develop a dental sterilizer which can sterilize most materials, such as metals, rubbers, and plastics, the sterilization effect of an atmospheric pressure non-thermal air plasma device was evaluated. MATERIALS AND METHODS. After inoculating E. coli and B. subtilis the diamond burs and polyvinyl siloxane materials were sterilized by exposing them to the plasma for different lengths of time (30, 60, 90, 120, 180 and, 240 seconds). The diamond burs and polyvinyl siloxane materials were immersed in PBS solutions, cultured on agar plates and quantified by counting the colony forming units. The data were analyzed using one-way ANOVA and significance was assessed by the LSD post hoc test (α=0.05). RESULTS. The device was effective in killing E. coli contained in the plasma device compared with the UV sterilizer. The atmospheric pressure non-thermal air plasma device contributed greatly to the sterilization of diamond burs and polyvinyl siloxane materials inoculated with E. coli and B. subtilis. Diamond burs and polyvinyl siloxane materials inoculated with E. coli was effective after 60 and 90 seconds. The diamond burs and polyvinyl siloxane materials inoculated with B. subtilis was effective after 120 and 180 seconds. CONCLUSION. The atmospheric pressure non-thermal air plasma device was effective in killing both E. coli and B. subtilis, and was more effective in killing E. coli than the UV sterilizer.

상온대기압 질소 및 공기 플라즈마가 의치상용 레진의 표면 특성과 살균효과에 미치는 영향

서혜연 ( Hye Yeon Seo ),유은미 ( Eun Mi Yoo ),최유리 ( Yu Ri Choi ),김수화 ( Soo Hwa Kim ),김광만 ( Kwang Mahn Kim ),김경남 ( Kyoung Nam Kim ) 한국치위생학회(구 한국치위생교육학회) 2014 한국치위생학회지 Vol.14 No.5

Objectives : The purpose of this study was to investigate the effect of non-thermal atmospheric pressure plasma jet(NTAPPJ) on surface properties and Streptococcus mutans disinfection of denture base resin. Methods : Self-cured denture base resin (Jet denture repair resin, Lang dental Mfg, co., USA) was used to make specimen(12 mm × 2 mm). To observe surface change before and after plasma process, surface roughness and contact angle were measured. For sterilization experiments, the surfaces of specimens were treated with nitrogen and air NTAPPJ for 1 minute after S. mutans was inoculated on the material surfaces. Results : Before plasma process, surface roughness of denture base resin was 0.21 §­± 0.02 §­. After air and nitrogen NTAPPJ process, surface roughness was 0.19 §­± 0.03 §­and 0.18 §­± 0.01 §­respectively. There was no significant difference(p>0.05). Contact angle of control group without plasma process was 83.81ß± 3.14ß, while after plasma treatment, contact angles of air NTAPPJ and nitrogen NTAPPJ groups were 63.29ß± 2.27ßand 46.68ß± 5.82ßrespectively. The result showed a significant decrease in contact angle after plasma process(p<0.05). Compared to the control group 6020.33(CFU/mL) without plasma process, CFU decreased significantly after air NTAPPJ 90.75(CFU/mL) and nitrogen NTAPPJ 80.25(CFU/mL) treatment(p<0.05). Conclusions : It was considered that NTAPPJ can be used for denture disinfection without changing surface properties of materials.

ELECTRON TEMPERATURE ESTIMATION OF NON-THERMAL ATMOSPHERIC-PRESSURE NEON AND OXYGEN ADMIXTURE PLASMA JET BY CONVECTIVE WAVE PACKET MODEL

Jirapong SORNSAKDANUPHAP,Pradoong SUANPOOT,Young June Hong,Bhagirath Ghimire,Guangsup CHO,EunHa CHOI 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.2

Sequential Deposition of Hexamethyldisiloxane and Benzene in Non-Thermal Plasma Adhesion to Dental Ceramic

한금준,김재훈,김창근,정성노,전배혁,조병훈 한국고분자학회 2013 Macromolecular Research Vol.21 No.10

According to the types of dental ceramic, various surface treatment techniques have been tried for adhesive cementation, but each treatment has its shortcomings. A simple and universal surface treatment for intraoral and chair-side adhesion promotion is needed for resin cements. This study investigated whether hexamethyldisiloxane (HMDSO) and benzene, when deposited using a low-power non-thermal atmospheric pressure dielectric barrier discharge jet, were effective precursor monomers in dental ceramic adhesion. Their effect on adhesion was evaluated with shear bond strength test (SBS), contact angle measurement, X-ray photoelectron spectroscopy, and Fourier transform infra-red spectrophotometer in an attenuated total reflectance mode. The bonded interfaces and fractured surfaces were evaluated using a scanning electron microscope. Plasma polymerization of HMDSO resulted in formation of a siloxane network (Si2 peak), but its surface hydrophobicity hindered adhesive wetting. Additional deposition of benzene onto the HMDSO-coated ceramic surface increased the C1 peak, which partly corresponded to the C=C double bonds, and the hydrophilic peaks (C-O, C=O and O-C=O bonds). Benzene deposition itself improved the adhesion between the ceramic and the overlying adhesive through the chemical interaction of C=C double bonds and the increased hydrophilic groups. Additional deposition of HMDSO before benzene mediated the chemical adhesion of benzene to the ceramic surface and led to an additional increase of bond strength. Plasma polymerization of benzene and HMDSO/benzene increased the bond strength of composite resin to dental ceramic by improving wettability and adaptation with hydrophilic ether, carbonyl and ester groups, and chemical bonding with active species, such as C=C double bonds and silanol groups.