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DNA methylation-specific multiplex assays for body fluid identification.
An, Ja Hyun,Choi, Ajin,Shin, Kyoung-Jin,Yang, Woo Ick,Lee, Hwan Young Springer International ; Springer 2013 International journal of legal medicine Vol.127 No.1
<P>Recent advances in whole-genome epigenetic analysis indicate that chromosome segments called tissue-specific differentially methylated regions (tDMRs) show different DNA methylation profiles according to cell or tissue type. Therefore, body fluid-specific differential DNA methylation is a promising indicator for body fluid identification. However, DNA methylation patterns are susceptible to change in response to environmental factors and aging. Therefore, we investigated age-related methylation changes in semen-specific tDMRs using body fluids from young and elderly men. After confirming the stability of the body fluid-specific DNA methylation profile over time, two different multiplex PCR systems were constructed using methylation-sensitive restriction enzyme PCR and methylation SNaPshot, in order to analyze the methylation status of specific CpG sites from the USP49, DACT1, PRMT2, and PFN3 tDMRs. Both multiplex systems could successfully identify semen with spermatozoa and could differentiate menstrual blood and vaginal fluids from blood and saliva. Although including more markers for body fluid identification might be necessary, this study adds to the support that body fluid identification by DNA methylation profiles could be a valuable tool for forensic analysis of body fluids.</P>
Ahn, Chul Won,Kwon, Do Young,Jun, Doo Sung,Lee, Yoo Min,Kim, Young Chul Springer-Verlag ; Springer 2015 Amino acids Vol.47 No.6
<P>We determined the alterations in metabolic conversion of cysteine into glutathione and taurine in liver of rats treated with ethanol acutely. Ethanol treatment reduced cysteine as well as glutathione levels in liver for 24 h. However, cysteine dioxygenase was up-regulated rapidly, and hypotaurine/taurine levels were significantly higher than those found in the saline-treated rats. It is therefore suggested that enhancement of cysteine catabolism into taurine contributes to the depletion of hepatic glutathione, which could exacerbate the ethanol-induced oxidative liver injury.</P>
Acupuncture for the treatment of cancer pain: a systematic review of randomised clinical trials.
Choi, Tae-Young,Lee, Myeong Soo,Kim, Tae-Hun,Zaslawski, Christopher,Ernst, Edzard Springer International ; Springer 2012 SUPPORTIVE CARE IN CANCER Vol.20 No.6
<P>Controlling cancer-related pain is an important component in the palliative care of cancer patients. The objective of this review was to assess the effectiveness of acupuncture for treating cancer pain.</P>
Park, Jin-Hee,Bae, Sun Hyoung,Jung, Yong Sik,Jung, Young-Mi Springer International ; Springer 2015 Supportive Care in Cancer Vol.23 No.9
<P>The aim of this study was to evaluate the psychometric properties of the Korean version of the Functional Assessment of Cancer Therapy-Cognitive scale (FACT-Cog) in patients with breast cancer in Korea.</P>
Segmental hair analysis and estimation of methamphetamine use pattern.
Han, Eunyoung,Yang, Heejin,Seol, Ilung,Park, Yunshin,Lee, Bongwoo,Song, Joon Myong Springer International ; Springer 2013 International journal of legal medicine Vol.127 No.2
<P>The aim of this study was to investigate whether the results of segmental hair analysis can be used to estimate patterns of methamphetamine (MA) use. Segmental hair analysis for MA and amphetamine (AP) was performed. Hair was cut into the hair root, consecutive 1?cm length segments and 1-4?cm length segments. Whole hair was also analyzed. The hair samples were incubated for 20?h in 1?mL methanol containing 1?% hydrochloric acid after washing the hair samples. Hair extracts were evaporated and derivatization was performed using trifluoroacetic anhydride in ethylacetate at 65?C for 30?min. Derivatized extract was analyzed by gas chromatography/mass spectrometry. The 15 subjects consisted of 13 males and two females and their ages ranged from 25 to 42 (mean, 32). MA and AP concentrations in the whole hair ranged from 3.00 to 105.10?ng/mg (mean, 34.53) and from 0.05 to 4.76?ng/mg (mean, 2.42), respectively. Based on the analysis of the 1?cm length segmental hair, the results were interpreted in a way to distinguish between continuous use of MA (n?=?10), no recent but previous use of MA (n?=?3), and recent but no previous use of MA (n?=?2). Furthermore, the individuals were interpreted as light, moderate, and heavy users based on concentration ranges previously published.</P>
Shim, J.K.,Choi, Y.S.,Yoo, K.J.,Kwak, Y.L. Springer International ; Springer-Verlag New York 2009 European journal of cardio-thoracic surgery Vol.36 No.3
Although use of carbon dioxide (CO<SUB>2</SUB>) blower has been regarded safe during off-pump coronary bypass surgery (OPCAB), we experienced a case of right coronary artery ischaemia induced by retrograde CO<SUB>2</SUB> embolism originating from the opened obtuse marginalis artery during OPCAB. The spray pressure can exceed the diastolic pressure, especially during grafting at the lateral or posterior wall when haemodynamic compromise due to mechanical heart displacement is most severe. In this situation, CO<SUB>2</SUB> blowing at an incompletely slinged coronary arteriotomy site can result in retrograde migration of CO<SUB>2</SUB> into the ascending aorta causing coronary embolism of the right coronary artery. When signs of ischaemia on the right coronary artery are encountered during grafting of other coronary artery, although CO<SUB>2</SUB> blower has been used, gas embolism should also be considered as the cause and identified at the mid-oesophageal aortic valve long-axis view. When confirmed, the use of gas blower should immediately be discontinued and coronary perfusion pressure increased while allowing time for the CO<SUB>2</SUB> to be absorbed. In case of massive embolism, needle aspiration of the gas should also be considered.
Jung, Hee-Won,Kim, Jin Won,Kim, Ji-Yeon,Kim, Sun-Wook,Yang, Hyun Kyung,Lee, Joon Woo,Lee, Keun-Wook,Kim, Duck-Woo,Kang, Sung-Bum,Kim, Kwang-Il,Kim, Cheol-Ho,Kim, Jee Hyun Springer International ; Springer 2015 SUPPORTIVE CARE IN CANCER Vol.23 No.3
<P>The purpose of this study was to elucidate the effect of decreased muscle mass on the toxicity and survival of patients with colon cancer treated with adjuvant chemotherapy after surgery.</P>
Kim, Kyung-Yong,Kwon, Younghyuk,Bazarragchaa, Munkhtsetseg,Park, Ae-Ja,Bang, Hyowon,Lee, Won-Bok,Lee, Junyoung,Lee, Kwang-Ho,Kim, Bum-Joon,Kim, Kijeong Springer International ; Springer 2013 International journal of legal medicine Vol.127 No.1
<P>Allelic dropout due to stochastic variation in degraded small quantity DNA appears to be one of the most serious genotyping errors. Most methods require PCR replication to address this problem. The small amounts of valuable samples are often a limitation for such replications. We report a real-time PCR-based amelogonin Y (AMELY) allele dropout estimation model in an AMEL-based gender typing. We examined 915 replicates of AMELY-positive modern male DNA with varying amounts of DNA and humic acid. A male-specific AMEL fragment (AMELy) dropped out in 143 genuine male replicates, leading to gender typing errors. By graphing a scatter plot of the crossing point versus the end cycle fluorescence of the male replicates, a standard graph model for the estimation of the AMELy allele dropout was constructed with the dropout-prone and dropout-free zones. This model was then applied to ancient DNA (aDNA) samples. Nine samples identified as female were found in the dropout-prone zone; with higher DNA concentrations, six were shifted to the dropout-free zone. Among them, two female identifications were converted to male. All the aDNA gender was confirmed by sex-determination region Y marker amplification. Our data suggest that this model could be a basic approach for securing AMELy allele dropout-safe data from the stochastic variation of degraded inhibitory DNA samples.</P>
Hwang, In-Chang,Kim, Yong-Jin,Kim, Kyung-Hee,Lee, Seung-Pyo,Kim, Hyung-Kwan,Sohn, Dae-Won,Oh, Byung-Hee,Park, Young-Bae Springer International ; Springer-Verlag New York 2013 European journal of cardio-thoracic surgery Vol.43 No.1
<P>The prognostic value of B-type natriuretic peptide (BNP) for surgical outcome in patients with mitral regurgitation (MR) has not been studied. The purpose of this study was to determine the prognostic value of BNP in patients with chronic severe MR, undergoing mitral valve surgery.</P>