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RISS 인기검색어
- 검색키워드
- 저자 : Zhifei Chen (검색결과 5 건)
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Liu, Di,Zhang, Ting,Chen, Zhifei,Wang, Ying,Ma, Shuang,Liu, Jiyun,Liu, Jingbo The Korean Society of Ginseng 2017 Journal of Ginseng Research Vol.41 No.2
Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by $40{\mu}M$ $H_2O_2$. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of $14.48{\pm}4.04{\mu}M\;TE\;per\;{\mu}g/mL$). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.
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Di Liu,Ting Zhang,Zhifei Chen,Ying Wang,Shuang Ma,Jiyun Liu,Jingbo Liu 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.2
Background: Ginsenosides are the main pharmacological components of Panax ginseng root, which are thought to be primarily responsible for the suppressing effect on oxidative stress. Methods: 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and oxygen radical absorption capacity were applied to evaluate the antioxidant activities of the ginsenosides. Human embryonic kidney 293 (HEK-293) cells were incubated with ginsenosides extracted by pulsed electric field (PEF) and solvent cold soak extraction (SCSE) for 24 h and then the injury was induced by 40mM H2O2. The cell viability and surface morphology of HEK-293 cells were studied using MTS assay and scanning electron microscopy, respectively. Dichloro-dihydro-fluorescein diacetate fluorescent probe assay was used to measure the level of intracellular reactive oxygen species. The intracellular antioxidant activities of ginsenosides were evaluated by cellular antioxidant activity assay in HepG2 cells. Results: The PEF extracts displayed the higher 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and stronger oxygen radical absorption capacity (with an oxygen radical absorption capacity value of 14.48 4.04mM TE per mg/mL). The HEK-293 cell model also suggested that the protective effect of PEF extracts was dose-dependently greater than SCSE extracts. Dichloro-dihydro-fluorescein diacetate assay further proved that PEF extracts are more active (8% higher than SCSE extracts) in reducing intracellular reactive oxygen species accumulation. In addition, scanning electron microscopy images showed that the HEK-293 cells, which were treated with PEF extracts, maintained more intact surface morphology. Cellular antioxidant activity values indicated that ginsenosides extracted by PEF had stronger cellular antioxidant activity than SCSE ginsenosides extracts. Conclusion: The present study demonstrated the antioxidative effect of ginsenosides extracted by PEF in vitro. Furthermore, rather than SCSE, PEF may be more useful as an alternative extraction technique for the extraction of ginsenosides with enhanced antioxidant activity.
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Acoustic emission characterization and cracking characteristics of reinforced concrete pipe
Jianfeng Li,Linbing Wang,Dongcan Chen,Yalin Liu,Zhifei Gao,Wentao Wang,Hailu Yang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.9
Acoustic emission (AE) and digital image correlation (DIC) technology were used in this study to monitor the behavior of reinforced concrete pipe (RCP) in the optimized three-edge bearing test (TEBT). At the same time, the AE source location of different pipe sections was simulated. The results show that the whole compressive loading process for RCP could be divided into 3 to 4 stages. Both the AE counts and AE energy could well characterize the cracking of concrete, and AE energy was more sensitive to the breaking of steel bar. The amplitude and duration of AE signal produced by the breaking of steel bar were larger than those produced by the cracking of concrete. The AE frequency for concrete cracking was relatively scattered. The AE frequency for steel bar breaking was small and concentrated, and this frequency distribution showed an exponential decreasing trend. The results of optimized AE source location showed that the generation and development of cracks occur in the initial loading stage. The results of multi-index analysis showed that the cracks in the top (loading position) and the bottom (bearing position) of the RCP show that the cracks extend from the inner wall to the outer wall, while the cracks on the left and right sides perpendicular to the loading direction show that the cracks extend from the outer wall to the inner wall.
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