OSP 투자지능화 시스템의 고찰

오구영,임미숙,정재우,조용환 충북대학교 컴퓨터 정보통신 연구소 2000 컴퓨터정보통신연구 Vol.8 No.1

본 논문은 OSP(Outside Plant) 투자지능화 시스템개발을 위해서 국내외에서 개발하여 사용하고 있는 선로투자공급 시스템들에 대하여 관심을 두고, 관련된 시스템들을 조사하여 각각의 시스템의 특징 및 구성에 대하여 분석하였다. 이러한 분석결과를 토대로 국내 통신 사업체에서 통신망 계획 및 분석을 위해 앞으로 개발하여야 할 선로투자공급 모형에 대한 기준을 제시한다. This paper analysed systems that are developed as relevant systems for OSP. We focused their distinction and composition of each system which are developed and used to interior and overseas. This result is able to propose the standard model of a Intelligent System for OSP model which have to develop system for plan and analysis of communication network to telecommunication operator.

월경전 기분 변화와 출산후 불안-우울 증상간의 상관성

한창수,김용구,이낙우,김 탁,김해중,김 현,조숙행 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.1

To investigate the relationship between premenstrual mood changes and anxiety, and mood symptoms in the perinatal period , questionaire survey was carried out. There was no significant correlation between premenstrual symptom scale value and Edinburgh postpartum depression scale value. And theme was no relationship between premenstrual symptom scale value and state anxiety inventory scale value in the perinatal period. This findings suggest that premenstrual mood changes are not directly correlated with mood changes in the perinatal period.

cDNA Cloning and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes-Jocheon

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.

Effect of Dongchunghacho on Gene Expression in Aorta and Liver in ApoE -/- Mice Fed a High-fat/high-cholesterol Diet

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sook Jae Sea,Hong Ja Kim,Eunju Park,Gyeong Im Jeon,Hyun Jung Lee, 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

Molecular Cloning, Expression and Genomic Structure of Glyceraldehyde-3-Phosphate Dehydrogenase from the Entomopathogenic fungus, Paecilomyces tenuipes Jocheon-1

Nam Sook Park,Han Seok Kang,Seon Ku Kim,Yong Gyun Kim,Byung Uuk Cho,Teak Soon Shin,Keun Ki Kim,Hyun Chul Park,Hong Joo Son,Hong Gu Lee,Sang Mong Lee 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces tenuipes Jocheon-1. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. Paecilomyces tenuipes Jocheon-1 cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes Jocheon-1 was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDHis comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The cDNA encoding Pt-GAPDH was expressed as a 37 kDa polypeptide in baculovirus-infected insect Sf9 cells. The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA.

Evaluation of Effects of Soil Moisture Condition on the Growth of Medicinal Crops(Cnidium officinale Makino, Astragalus membranaceus Bunge)

Yong Goo Kim(김용구),Yong Il Kim(김용일),Eun song Lee(이은송),Sang Ku Lee(이상구),Kyung Sook Han(한경숙) 한국약용작물학회 2022 한국약용작물학회 학술대회논문집 Vol.2022 No.1

Cucurbitacin D induces cell cycle arrest and apoptosis by inhibiting STAT3 and NF-κB signaling in doxorubicin-resistant human breast carcinoma (MCF7/ADR) cells

Ku, Jin Mo,Kim, Soon Re,Hong, Se Hyang,Choi, Han-Seok,Seo, Hye Sook,Shin, Yong Cheol,Ko, Seong-Gyu Springer US 2015 MOLECULAR AND CELLULAR BIOCHEMISTRY - Vol.409 No.1

<P>Breast cancer is the most common cancer for women and is a major cause of mortality in women. Doxorubicin is a generally used chemotherapy drug for breast cancer. However, multidrug resistance of breast cancer interferes with the chemotherapy. We examined whether cucurbitacin D affects doxorubicin resistance of MCF7/ADR breast cancer cells. Cell viability was measured by MTT assay. Levels of p-STAT3, p-NF-κB, IκB, and caspases were measured by Western blot analysis. Nuclear staining of Stat3 and NF-κB was measured by immunocytochemistry. STAT3 and NF-κB transcriptional activity was detected by STAT3 and NF-κB luciferase reporter gene assays. Analysis of cell cycle arrest was performed by flow cytometry. Induction of apoptosis by cucurbitacin D was measured by Annexin V-FITC/propidium iodide assay. More than 90 % of MCF7/ADR cells lived upon treatment with doxorubicin for 24 h. However, upon treatment with cucurbitacin D, cell death was more than 60 %. Co-administration of cucurbitacin D and doxorubicin induced apoptosis, and G2/M cell cycle arrest, and inhibited upregulated Stat3 by doxorubicin on MCF7/ADR cells. Additionally, cucurbitacin D led to an increase in the IκBα level in the cytosol and a decrease in the p-NF-κB level in the nucleus. Finally, cucurbitacin D inhibited translocation of Stat3 and NF-κB and decreased transcriptional activity in the nucleus. Cucurbitacin D decreases cell proliferation and induces apoptosis by inhibiting Stat3 and NF-κB signaling in doxorubicin-resistant breast cancer cells. Cucurbitacin D could be used as a useful compound to treat adriamycin-resistant patients.</P>

Spatiotemporal distribution and genetic diversity of identified bat astroviruses in Korea

( Sook-young Lee ),( Ji-su Kim ),( Ki-dong Son ),( Yong-kwan Kim ),( Yong-sik Kim ),( Hye-sung Jeong ),( Weon-hwa Jheong ),( Jae-ku Oem ) 대한인수공통전염병학회 2018 창립총회 및 학술대회 초록집 Vol.2018 No.1

Bats have been identified as a natural reservoir for several potentially zoonotic viruses such as Rabies virus, Lyssa virus, Ebola virus, Marburg, SARS coronavirus, MERS coronavirus. However, current surveillance studies on bats are insufficient in Korea. Therefore, in this study, we performed a molecular and epidemiological investigation of bat viruses in Korea. A total of 363 bat samples (sixty-one oral swabs, 244 fecal samples, and fifty-eight bat carcasses) were collected at twenty-nine sites across sixteen provinces in Korea from January to September 2016. RNA viruses from bat samples were investigated by consensus primer-based reverse transcriptase polymerase chain reactions (RT-PCRs). Consequently, the partial RNA-dependent RNA polymerase (RdRp) gene of astroviruses (34/363 bats, 9.4%) was detected in bat samples by RT-PCR. Bat astroviruses were highly detected in Sunchang (8/13 bats, 61.5%), and in the samples collected in April (12/19 bats, 63.2%). The amino acid similarity of astroviral sequences identified from Korean bat samples was ≥ 46.6%. Specifically, the amino acid similarity of astrovirus haplotypes in Korean bats was ≥ 50.8%. In addition, the phylogenetic topology between astroviruses from different families of bats was shown to have a close relationship. Furthermore, phylogenetic analysis of the partial ORF2 sequence of representative bat astorviruses was found to have a maximum similarity of 73.3-74.8% compared to available bat astrovirus sequences. These results indicated a high probability of multiple-infection of several bat astrovirus species in individual bats, as well as the transmission of astroviruses across the bat families. However, the wide diversity of astroviral sequences appeared to have no significant correlation with bat species or the spatiotemporal distribution of Korean bat astroviruses. In bats, the co-infection and recombination of viruses has been occasionally reported. This suggests that the ecological characteristics of bats may increase the opportunities for recombination in various viruses. The movement of bats due to a decrease in the size of their habitats causes an increase in contact opportunities with domestic animals as well as humans; zoonotic pathogens in bats may then pose a potential risk to be transmitted to humans and domestic animals. Therefore, further surveillance studies of bat viruses are required due to the lack of clear knowledge on bat pathogens in South Korea. The subject is supported by the National Institute of Environmental Research of the Republic of Korea (No. 2016-01-033) and Korea Ministry of Environment (MOE) as "Public Technology Program based on Environmental Policy (No. 2016000210002)".

Interaction of glycopolymers with human hematopoietic cells from cord blood and peripheral blood

Lee, Yong-Su,Park, Keun-Hong,Kim, Tea-Sik,Kim, Jea-Myeoung,Sohn, In-Sook,Park, Jin-Ki,Chang, Won-Kyong,Kim, Dong-Ku Wiley Subscription Services, Inc., A Wiley Company 2008 Journal of biomedical materials research. Part A Vol.a86 No.4

<P>Polystyrene derivatives, poly[N-pvinylbenzyl-O-D-glucopyranosyl-(1-4)-D-glucoamide] (PV Maltose) and poly[N-p-vinylbenzyl-O-mannopyranosyl-(1-4)-D-glucoamide] (PV Mannose), which contain glucose and mannose moieties, respectively, have the specific binding ability with murine hematopoietic cells. In this study, we confirm the ability of these glycopolymers to interact specifically with human hematopoietic stem cells (HSCs) and mature cells derived from human cord blood (CB) and peripheral blood (PB). Using fluorescence isothiocyanate (FITC)-labeled glycopolymers, we observed that 98% to 93% of hematopoietic cells interacted very strongly with PV Mannose, and 63% of CB and 29% PB interacted with PV Maltose. Both glycopolymers bound better to cells from CB than from PB. Cytotoxic studies revealed that a 0.1 mM dose of PV Mannose induced apoptosis in 20% CB cells, in contrast to 3–5% PB cells. Furthermore, we demonstrated that all of CD34<SUP>+</SUP> HSCs of both origins bound specifically to PV Mannose, whereas 33–47% bound to PV Maltose. In addition, the majority of B cells (CD19<SUP>+</SUP>), T cells (CD3<SUP>+</SUP>), monocytes (CD14<SUP>+</SUP>), and erythrocytes (CD235a<SUP>+</SUP>) bound to PV Mannose, but a lower percentage interacted with PV Maltose. In vivo study, bone marrow, spleen, and liver tissues in NOD-SCID mice injected with PV Mannose conjugated CB, were detected PV Mannose positive hematopoietic cells. These data suggest that the use of PV Mannose and PV Maltose might be used for gene and drug delivery for hematopoietic cells and thus, may be useful in therapeutic settings. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008</P>

가공 방법 및 저장기간에 따른 대나무통 발효차의 성분 변화

이용호(Yong-Ho Lee),하기정(Gi-jeong Ha),김낙구(Nak-Ku Kim),이남숙(Nam-Sook Lee),손길만(Gil-Man Shon) 한국차학회 2013 한국차학회지 Vol.19 No.1

대나무통에 유념한 차 잎을 넣어 실온, 30℃, 40℃의 온도에서 12시간, 24시간, 36시간으로 조건을 달리하여 발효시켰으며, 발효 후에는 65℃ 온도에서 15일 동안 건조시켜 저장 30일부터 저장 90일까지 발효차의 성분변화를 비교분석 하였다. 발효차 저장 중 일반성분 함량 변화에서는 조단백질, 조회분, 조지방 함량이 발효온도, 발효시간, 저장기간에 따른 큰 변화는 보이지 않았으나, 수분 함량은 저장 전(3.1-5.3%) 저장 90일(5.9-8.6%)로 저장 기간이 경과할수록 함량이 증가하였다. 무기질 함량변화는 K>Ca>Mg>Mn>Na > Fe 순으로 높게 나타났다. pH 변화에서는 발효온도와 발효시간 및 저장기간이 경과할수록 큰 변화를 보이지 않았고, 총산 함량은 저장 전에 1.53-2.12%, 저장 30일 2.07-2.47%, 저장 60일 2.18-2.47%, 저장 90일 2.14-2.56%로 저장기간이 경과할수록 총산 함량이 증가하였다. 저장기간이 경과할수록 L(명도) 값은 높게 나타났으며 적색도(a)와 황색도(b)는 낮아지는 현상을 보였다. 가용성 고형분(brix°)의 변화는 저장 전과 저장 30일에 0.4-0.5 brix°로 낮게 나타났으나 저장 60일에서 저장 90일에서는 0.6 brix°로 높아지면서 일정한 brix°를 유지하였다. Gallic acid는 저장기간이 경과할수록 함량이 감소하였고, 저장 전과 발효온도 30℃, 24시간 발효에서 가장 높은 함량을 나타냈다. 카테킨류는 6종류가 검출되었으며, EGCG >ECG > C > GC > EGC > EC 순으로 높은 함량을 나타냈으며, 저장기간이 경과할수록 함량이 낮아지는 현상을 보였다. Caffeine의 경우 저장기간이 경과할수록 감소하였으며, 실온, 12시간 발효에서 가장 높은 함량을 나타냈다. 아미노산은 생잎과 발효 전부터 발효 후까지 발효시간에 상관없이 실온에서 90일 저장 시와 30℃, 40℃에서 30일 저장 하였을 때 ammonium chloride가, 상온과 30℃에서 30일간 저장 시 glycine이, 40℃에서 90일 저장 시 alanine이 가장 많이 검출되었다. 차의 수색에 관여하는 성분 중에서 차의 품질을 결정하는 클로로필 함량은 저장기간이 경과할수록 함량이 감소하는 경향을 보였다. 기호도 조사에서는 40℃에서 12시간 발효 시켰을 때 선호도가 가장 높게 나타났으며, 발효시간이 짧을수록 선호도가 높게 나타났다. Abstract Tea leaves were prepared using a fermentation method for 12 hours, 24 hours, and 36 hours with 30℃ and 40℃ temperature in a bamboo tube container. After fermentation, changes in the general components of tea leaves dehydrated in 65℃ for 15 days were analyzed from before storage to the 90th day at 30 day intervals. In terms of the general content, the moisture was found to be proportional to the storage period, which was 3.1-5.3% before storage and 5.9-8.6% at the 90th day of storage. The mineral content was K, Ca, Mg, Mn, Na and Fe arranged in the order of their concentration. The pH was 4.80-5.10 before storage, which decreased to 4.45-4.62 at the 30^th day of storage, and was maintained at those levels until the 90^th day after storage. The total acid was 1.53%-1.74% before storage, and 2.07-2.47% at the 30th day to 90^th day of storage. The soluble solid content was 0.4-0.5 brix° before storage, which was maintained at 0.6 brix° for 60-90 days. For the chromaticity, the brightness was proportional to the storage period, whereas the reddish and yellowish rates were inversely proportional to the storage period. The gallic acid content was inversely proportional to the storage period, which shows the highest value for the 12 hour fermentation processing time. Six types of catechins were detected in the experiment and the order according to the amount was EGCG, ECG, EC, EGC and GC. In general, the catechin content is inversely proportional to the storage period. The caffeine content was highest at 12 hours at normal temperatures. Finally, the fermentation conditions of the highest preference were 40℃ for 12 hours, which is inversely proportional to the storage period.