침구경락 음양론의 새로운 발전, 기능적 뇌 척주요법 FCST

인창식 ( Chang Shik Yin ),고형균 ( Hyeong Gyun Koh ),이영진 ( Young Jin Lee ),전세일 ( Sae Il Chun ),이영준 ( Young Jun Lee ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.4

Objectives: Functional Cerebrospinal Therapy (FCST) is a new physiologic therapeutics developed in Korea as a meridian yinyang balance approach. The theory of yinyang balance has been at the core of health enhancement approach of meridian and acupuncture medicine ever since its start. Methods: Introductory overview of FCST is presented in relation with meridian yinynag balance theory. Results: As the temporomandibular joint (TMJ) and related tissues have direct interconnection with brainstem proprioceptive or motor systems and the face is where all the meridians converge, FCST applies a fine adjustment of the posture of TMJ as a treatment tool for neurologic conditions or meridian imbalances. Conclusions: Highly sophisticated diagnostic and therapeutic techniques to adjust various subset aspects of yinyang balance are developed within FCST, which is supposed to be one of major contributions to natural healing.

Risk factors affecting amputation in diabetic foot

Jun Ho Lee,Ji Sung Yoon,Hyoung Woo Lee,Kyu Chang Won,Jun Sung Moon,Seung Min Chung,Yin Young Lee 영남대학교 의과대학 2020 Yeungnam University Journal of Medicine Vol.37 No.4

Background: A diabetic foot is the most common cause of non-traumatic lower extremity amputations (LEA). The study seeks to assess the risk factors of amputation in patients with diabetic foot ulcers (DFU). Methods: The study was conducted on 351 patients with DFUs from January 2010 to December 2018. Their demographic characteristics, disease history, laboratory data, ankle-brachial index, Wagner classification, osteomyelitis, sarcopenia index, and ulcer sizes were considered as variables to predict outcome. A chi-square test and multivariate logistic regression analysis were performed to test the relationship of the data gathered. Additionally, the subjects were divided into two groups based on their amputation surgery. Results: Out of the 351 subjects, 170 required LEA. The mean age of the subjects was 61 years and the mean duration of diabetes was 15 years; there was no significant difference between the two groups in terms of these averages. Osteomyelitis (hazard ratio [HR], 6.164; 95% confidence interval [CI], 3.561−10.671), lesion on percutaneous transluminal angioplasty (HR, 2.494; 95% CI, 1.087−5.721), estimated glomerular filtration rate (eGFR; HR, 0.99; 95% CI, 0.981−0.999), ulcer size (HR, 1.247; 95% CI, 1.107−1.405), and forefoot ulcer location (HR, 2.475; 95% CI, 0.224−0.73) were associated with risk of amputation. Conclusion: Osteomyelitis, peripheral artery disease, chronic kidney disease, ulcer size, and forefoot ulcer location were risk factors for amputation in diabetic foot patients. Further investigation would contribute to the establishment of a diabetic foot risk stratification system for Koreans, allowing for optimal individualized treatment.

A HYBRID ENERGY STORAGE SYSTEM COMBINING PSP AND CAES

Jun lian Yin,De zhong Wang,Yu-Taek Kim,Young Ho Lee 한국신재생에너지학회 2012 AFORE Vol.2012 No.-

MODIFIED MULTIPLICATIVE UPDATE ALGORITHMS FOR COMPUTING THE NEAREST CORRELATION MATRIX

Yin, Jun-Feng,Huang, Yumei The Korean Society for Computational and Applied M 2012 Journal of applied mathematics & informatics Vol.30 No.1

A modified multiplicative update algorithms is presented for computing the nearest correlation matrix. The convergence property is analyzed in details and a sufficient condition is given to guarantee that the proposed approach will not breakdown. A number of numerical experiments show that the modified multiplicative updating algorithm is efficient, and comparable with the existing algorithms.

Analysis of the peptides detected in atopic dermatitis and various inflammatory diseases patients-derived sera

Yin, Shang-Jun,Cho, Ick-Hyun,Yang, Hee Seung,Park, Yong-Doo,Yang, Jun-Mo Elsevier 2018 International journal of biological macromolecules Vol.106 No.-

<P><B>Abstract</B></P> <P>Serum proteomics has been applied for the discovery and analysis of biomarkers related to human disease. Serum is an optimal source to identify proteins derived from diseased-tissue compartments. We recently established an integrative method to analyze highly basic proteins that remain unresolved by the general 2D-PAGE method. In this follow-up study, we successfully detected several disease-associated proteins from sera samples obtained from patients with atopic dermatitis (AD). After proteomic analyses, target proteins were validated from AD patient-derived sera using ELISA or Western blotting methods We detected zinc finger CCHC domain containing 10 (ZCCHC10), peptidoglycan recognition protein L (PGRP-L), kininogen, α-1-antitrypsin, and hornerin proteins that are dysregulated in AD patient sera samples, which suggest effective approaches to methodologically analyze the serum proteome. Thus, the integrated proteomic method approach described here could be applicable for the detection of proteins associated with other human diseases. Our present study provides new insights into optimized serum proteomic techniques to understand systemic events of AD.</P>

Preparation of a cyanine-based fluorescent probe for highly selective detection of glutathione and its use in living cells and tissues of mice

Yin, Jun,Kwon, Younghee,Kim, Dabin,Lee, Dayoung,Kim, Gyoungmi,Hu, Ying,Ryu, Ji-Hwan,Yoon, Juyoung Nature Publishing Group 2015 NATURE PROTOCOLS -ELECTRONIC EDITION- Vol.10 No.11

Glutathione (GSH) is a major endogenous antioxidant that has a central role in cellular defense against toxins and free radicals. This protocol describes the preparation of CPDSA, a cyanine-based near-infrared (NIR) fluorescent probe for the detection of GSH in cells and in vivo. CPDSA is prepared with high yield through a simple two-step process. The first step is to react commercially available IR-780 iodide with excess anhydrous piperazine in anhydrous N,N-dimethyl formamide at 85 °C to form cyanine-piperazine (CP). The second step is the sulfonylation of CP with dansyl chloride in anhydrous dichloromethane. CPDSA selectively detects GSH in cells, and it has been shown to not react with other biothiols such as cysteine (Cys) and homocysteine (Hcy). This probe can also be used to monitor the GSH level of mouse bone marrow–derived neutrophils (BMDNs). The preparation of probe CPDSA takes 2 d, and experiments in cells and mice take 12–13 d.

Fluorescent probes and bioimaging: alkali metals, alkaline earth metals and pH

Yin, Jun,Hu, Ying,Yoon, Juyoung The Royal Society of Chemistry 2015 Chemical Society reviews Vol.44 No.14

<P>All living species and life forms have an absolute requirement for bio-functional metals and acid–base equilibrium chemistry owing to the critical roles they play in biological processes. Hence, a great need exists for efficient methods to detect and monitor biometals and acids. In the last few years, great attention has been paid to the development of organic molecule based fluorescent chemosensors. The availability of new synthetic fluorescent probes has made fluorescence microscopy an indispensable tool for tracing biologically important molecules and in the area of clinical diagnostics. This review highlights the recent advances that have been made in the design and bioimaging applications of fluorescent probes for alkali metals and alkaline earth metal cations, including lithium, sodium and potassium, magnesium and calcium, and for pH determination within biological systems.</P> <P>Graphic Abstract</P><P>This review highlights the recent advances that have been made in the design and bioimaging applications of fluorescent probes for alkali metals, alkaline earth metal cations and for pH determination within biological systems. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c4cs00275j'> </P>

Cyanine-Based Fluorescent Probe for Highly Selective Detection of Glutathione in Cell Cultures and Live Mouse Tissues

Yin, Jun,Kwon, Younghee,Kim, Dabin,Lee, Dayoung,Kim, Gyoungmi,Hu, Ying,Ryu, Ji-Hwan,Yoon, Juyoung American Chemical Society 2014 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.136 No.14

<P>Glutathione (GSH) plays a crucial role in human pathologies. Near-infrared fluorescence-based sensors capable of detecting intracellular GSH in vivo would be useful tools to understand the mechanisms of diseases. In this work, two cyanine-based fluorescent probes, <B>1</B> and <B>2</B>, containing sulfonamide groups were prepared. Evaluation of the fluorescence changes displayed by probe <B>1</B>, which contains a 2,4-dinitrobenzenesulfonamide group, shows that it is cell-membrane-permeable and can selectively detect thiols such as GSH, cysteine (Cys), and homocysteine (Hcy) in living cells. The response of <B>1</B> to thiols can be reversed by treatment with <I>N</I>-methylmaleimide (NMM). Probe <B>2</B>, which possesses a 5-(dimethylamino)naphthalenesulfonamide group, displays high selectivity for GSH over Cys and Hcy, and its response can be reversed using NMM. The potential biological utility of <B>2</B> was shown by its use in fluorescence imaging of GSH in living cells. Furthermore, probe <B>2</B> can determine changes in the intracellular levels of GSH modualated by H<SUB>2</SUB>O<SUB>2</SUB>. The properties of <B>2</B> enable its use in monitoring GSH in vivo in a mouse model. The results showed that intravenous injection of <B>2</B> into a mouse generates a dramatic image in which strong fluorescence is emitted from various tissues, including the liver, kidney, lung, and spleen. Importantly, <B>2</B> can be utilized to monitor the depletion of GSH in mouse tissue cells promoted by excessive administration of the painkiller acetaminophen. The combined results coming from this effort suggest that the new probe will serve as an efficient tool for detecting cellular GSH in animals.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/2014/jacsat.2014.136.issue-14/ja412628z/production/images/medium/ja-2013-12628z_0013.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ja412628z'>ACS Electronic Supporting Info</A></P>

Metabolic responses and arginine kinase expression of juvenile cuttlefish (<i>Sepia pharaonis</i>) under salinity stress

Yin, Shang-Jun,Zhang, Linmeng,Zhang, Lili,Wan, Jiaxin,Song, Wei,Jiang, Xiamin,Park, Yong-Doo,Si, Yue-Xiu Elsevier 2018 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES Vol.113 No.-

<P><B>Abstract</B></P> <P>The pharaoh cuttlefish <I>Sepia pharaonis</I> is particularly sensitive to environmental changes in its breeding environment. The breeding of <I>S</I>. <I>pharaonis</I> larvae was carried out in different salinities for 48h, and the changes in survival rate, histological structure, energy metabolism, and anti-oxidative stress parameters were investigated and correlated with arginine kinase (AK) expression changes in muscle and liver tissues. The suitable salinity for larvae cultivation ranged from 24 to 30‰, and the survival rate showed a significant decline at 21‰ salinity. Histological observations of muscle and liver showed that changes in salinity and osmotic pressure had an adverse effect on tissue structure. Measurements of glycogen and lactic acid levels suggested that <I>S</I>. <I>pharaonis</I> could dynamically adjust energy metabolism to provide additional energy under unsuitable salinity. The protein levels and enzyme activities of AK in muscle significantly increased at 21‰ salinity. The results were consistent with prompt replenishment of phosphoarginine stores during salinity stress to maintain a dynamic ATP balance, suggesting that AK plays an important role in the regulation of energy metabolism. This study provides insight into metabolic changes during salinity stress and sheds light on the functional role of AK in <I>S</I>. <I>pharaonis</I>.</P>

Research on Traditional Culture Transmission in Digital Games Based on the IP Economic Model

Yin Jun,Zhou Xuanyi 한중경제문화학회 2023 한중경제문화연구 Vol.23 No.-