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      • The combined analysis of electrical penetration graph and video observation of behaviors by the Green Peach aphid, Myzus persicae

        Hyoyoung Jang,Hyojoong Kim,Yikweon Jang 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

        Plant penetration by aphids can be monitored electrically by the electrical penetration graph (EPG) technique. To confirm whether some behaviors are correlated to specific graph pattern in EPG, we analyzed the two synchronized data, EPG and video records. We recorded electrical penetration graph (EPG) and behaviors of aphids simultaneously. Then we compared the behaviors of aphids with the recorded EPG waveforms in order to match their visible behaviors and invisible behaviors with stylet. The visible behaviors were categorized for walking, wagging, honeydew production, and reproduction. When the aphids were generally motionless, the EPG denoted feeding-related waveforms (E1, E2, F and G). Whereas, probing waveforms (B and pd) frequently occurred when they were wagging. We aim to present the correlation between observed behaviors and EPG patterns.

      • No Effect of the Acoustic Stimulus on the Reproductive Rate in Myzus persicae

        Soojin Jang,Yikweon Jang 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.10

        An aphid usually stays at one feeding site for a long time for development and reproduction. The green peach aphids, Myzus persicae (Sulzer), feed consistently and regularly throughout all stages of nymphs and adult at one feeding site. In M. persicae, honey dew production, which indicates a state of feeding, occurs at regular intervals within a given stage, and moving, which may be related to escape or dispersal, interrupts feeding, The results of the playback experiments showed that acoustic stimuli with frequencies of 100 and 10000 Hz were effective in inducing feeding repression in M. persicae. That is, HDP occurred less often, and MV occurred more often and longer under acoustic stimuli. In this study, we tested whether the acoustic stimulus effective for inducing feeding suppression decreased reproductive rate in M. persicae. A group of 20 aphids were placed in a host plant and was subject to the acoustic stimulus with two frequency components, 100 and 10000 Hz, for a given time (1, 3, 6, and 12 hours) each day for four days. The result of this experiment showed that the acoustic stimulus did not affect the reproductive rate, regardless of exposure time, in M. persicae.

      • Lifetime Calling Patterns and Tactics in the Field Cricket Teleogryllus emma (Orthoptera: Gryllidae)

        Soojin Jang,Yikweon Jang,Jae Chun Choe 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

        Males of many cricket species produce calling songs to attract receptive, conspecific females. Calling songs which affected by calling pattern are critical for male mating success, since male with no or limited calling songs are unable to attract female crickets. Investigating among- and within-individual variation in calling pattern may reveal the presence of alternative mating tactics such as attracting females by producing calling songs or being satellites in which males intercept females who are attracted to calling males. In this study, I investigated patterns of calling song production during the entire adulthood of 32 laboratory-reared and 24 field-captured individuals of Teleogryllu emma. Using recording system which can record acoustic signals from up to 32 individuals simultaneously, I measured daily calling output (DCO) and total calling output (TCO) in addition to acoustic parameters of calling songs and classified T.emma males into consistent and inconsistent singers based on the consistency of singing. Consistent singers lived longer, sang longer both in a day and during the lifetime than the inconsistent singers. These results suggest that the consistent singers in this study may be considered to employ the calling tactic. In lab-reared individuals, there seemed to be a trade-off between increasing pulse duration and length of phrases and ling chirp although older males produced calling songs with longer ling chirps and more multiple phrases.

      • Concepts and Methods of Large-scale Ecological Data Collection for a Citizen Science Project

        Jaeyeon Kang,Eunjae Jang,Yikweon Jang 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

        Collection of ecological data such as the temporo-spatial distribution of a species is very difficult, due to broad distribution over large areas, phenology, and lack of resources for field survey. Citizen science, which is a cooperative scientific endeavor between researchers and interested citizens, is ideal for collecting large-scale ecological data. However, lack of proper equipment, species identification, and/or communication between researchers and participants are hindrance for a successful citizen science project. Here, we introduce the concept and methods of large-scale ecological data collection using smartphone apps. Most of the ecological data typically consist of sound or video recording, picture, geographic coordinate, and notes. There are several apps that can collect some or all of these ecological data. Furthermore, the result of a survey can be reported to researchers using Google Docs. The data collected by non-specialists can be validated by cross-checking of the survey report by Google Docs and the ecological data sent by apps. Finally, we report the results of a citizen science project in which temporo-spatial distributions of cicada species in Korea were studied via smartphone apps and Google Docs.

      • Acoustic competition in the multi-species cicada choruses

        Yikweon Jang,Jae Yeon Kang,Nguyen Quynh Hoa 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10

        Five species of the subfamily Cicadinae are typically found in urban areas of central Korea, including Cryptotympana atrata, Graptopsaltria nigrofuscata, Hyalessa fuscata, Meimuna mongolica, and Meimuna opalifera. These species form multi-species choruses. We hypothesized that there is a negative relationship between dominance of choruses among cicada species. We tested this hypothesis by conducting the 24-h recording experiments in four localities: Gawcheon, Jamsil, Ewha University, and Yangpyeong. Based on the enumeration surveys of exuviae, the results of 24-h recordings showed that choruses of C. atrata and H. fuscata were negatively correlated with other in Jamsil and Ewha University, but were positively correlated with other in Gawcheon and Yangpyeong. Thus, it seemed unclear cicada species competed with each other negatively over. Males of C. atrata produced calling songs usually during the day at temperatures typically higher than 28 C°. However, males of H. fuscata produced calling songs throughout the day at a wide range of temperatures. As a result, the chorus of C. atrata dominated during the day, but that of H. fuscata in urban areas where the densities of C. atrata were sufficiently high.

      • What Determines Densities of Cicada Species in Central Korea?

        Yikweon Jang,Tae Eun Kim,Seung-Yoon Oh 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

        In some cicada species, male calling songs are so loud that they become a nuisance to city dwellers in Korea. To understand the abundance of cicada species in central Korea, we conducted complete enumeration surveys of exuviae in Hyalessa fuscata, Cryptotympana atrata, Meimuna spp, and Graptopsaltria nigrofuscata. Exuviae collection was conducted in three representative habitats in central Korea: metropolitan, suburban, and country. We collected the exuviae twice with a 10-day interval between samplings in August 2010. Resource-weighted density of each species was calculated based on the area and the number of trees. H. fuscata was the dominant species in all three regions. Resource-weighted densities in metropolitan and suburban regions were much higher than those in the country region, due to H. fuscata and C. atrata. The results of the multivariate general linear models showed that region, date, and the interaction between these two variables were all significant for population densities of cicada species. Unlike the resource-weighted densities, the organism-weighted densities that indicated the intensity that an individual had to share its host with others were much larger than tree-weighted densities for cicada exuviae. Furthermore, there was no significant difference in organism-weighted densities among habitats. The difference between resource-weighted and organism-weighted densities implied that larger proportions of trees were not used by cicada juveniles in the country. Thus, the distributions and abundance of tree host species could be an important factor for cicada density in Korea.

      • Individual variation in aggressiveness against conspecific advertisement signals in Gryllus rubens (Orthoptera: Gryllidae)

        Yikweon Jang 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

        Consistent individual difference in a behavior is recognized as an important evolutionary force in niche variation and may be a personality trait, an inherent property of the individual. To understand whether aggressiveness is a personality trait, independent of resource holding potential (RHP) and resource value in animal aggression assumed by game theory models, I determined individual variation in aggressiveness by measuringboth positive phonotactic and acoustic responses to the broadcast stimulus repeatedly over the three-week period in males of Gryllus rubens. Factors tested included amplitude levels of the broadcast stimulus, body size, wing dimorphism, and age. The analyses of the generalized linear mixed models revealed that RHP factors such as age, wing dimorphism, and body size, were not significant for both positive phonotactic and acoustic responses. However, there seemed to be more aggressive responses to higher levels of amplitudes. The significant variance-covariance structures of the models indicated that some individuals consistently aggressive or nonagressive during the experimental periods. Because potential factors for resource values were controlled, and because potential RHP factors would not explain variation in aggressive responses, I concluded that aggressiveness is a personality trait that is not characterized by factors that I tested.

      • Inhibition of feeding behaviors using acoustic stimuli in aphids:Electrical penetration graph analyses

        Hyoyoung Jang,Amanda Wee Huixin,Sim Sze Ching Danielle,Yeo Qin Yi,Hyojoong Kim,Yikweon Jang 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.05

        Aphids feed on host plants by penetrating the stems or leaves with stylets. The feeding behavior of aphids consists of probing, penetration, salivation, and sap ingestion. To assess the effects of sound on feeding behavior, we monitored the stylet activity of the green peach aphid, Myzus persicae (Sulzer), using electrical penetration graph (EPG). The use of EPG was critical for determining the stage, frequency, and duration of feeding in aphids. We played back three acoustic stimuli of sine waves with frequencies of 100, 1000, and 5000 Hz to adult aphids. In the sound treatment group, the frequencies of probing, penetration, and salivation increased, whereas the duration of sap ingestion decreased. The results of EPG revealed that the acoustic stimuli may restrict aphid feeding by inhibiting sap ingestion.

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