Kinetics of Enzymatic Synthesis of L-ascorbyl Acetate by Lipozyme TLIM and Novozym 435

Dong-Hao Zhang,Ya-Qiong Li,Chao Li,Yv-Qin Lv,Yang-L 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1

L-ascorbyl acetate was synthesized through lipase-catalyzed esterification using Lipozyme TLIM and Novozym 435. Four solvents, including methanol, ethanol,acetonitrile, and acetone were investigated for the reaction,and acetone and acetonitrile were found to be suitable reaction media. The influences of several parameters such as water activity (aw), substrate molar ratio, enzyme loading,and reaction temperature on esterification of Lascorbic acid were systematically and quantitatively analyzed. Through optimizing the reaction, lipase-catalyzed esterification of L-ascorbic acid gave a maximum conversion of 99%. The results from using Lipozyme TLIM and Novozym 435 as biocatalysts both showed that aw was an important factor for the conversion of L-ascorbic acid. The effect of pH value on lipase-catalyzed L-ascorbic acid esterification in acetone was also investigated. Furthermore,results from a kinetic characterization of Lipozyme TLIM were compared with those for Novozym 435, and suggested that the maximum reaction rate for Lipozyme TLIM was greater than that for Novozym 435, while the enzyme affinity for substrate was greater for Novozym 436.

Effect of Rare Earth Elements on Proliferation and Fatty Acids Accumulation of 3T3-L1 Cells

He, M.L.,Yang, W.Z.,Hidari, H.,Rambeck, W.A. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.1

The present study including two experiments was designed to determine the effect of media containing different rare earth elements (REE) on proliferation and fatty acids accumulation in 3T3-L1 cell cultures. In Experiment 1, 3T3-L1 preadipocytes in 96-well plates ($1.5{\times}10^4cells/ml$) were cultured with Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) for 24 h. Then the media were changed to the following 10 different media for 48 h: DMEM containing 10% FBS for the control; the above media containing $5{\mu}M$, $10{\mu}M$ or $15{\mu}M$ of $LaCl_3$, $CeCl_3$ or the mixture of these REE chlorides. The proliferation rate of the cells was measured and compared by a non-isotope method-XTT method. In Experiment 2 the cells in 24-well plates ($1.5{\times}10^4cells/ml$) were cultured in DMEM containing 10% FBS for 7 days until confluent and then were changed to above DMEM containing dexamethasone, methyl-isobutylxanthine and insulin (DMI) for two days. Afterwards the media were changed to the 10 different media with REE supplements as in Experiment 1 and cultured for 6 days. The cells were then harvested for fatty acids analysis by gas chromatography. It was found that supplementation of La (5, 10 and $15{\mu}M$), Ce ($5{\mu}M$ and $15{\mu}M$) and the mixture REE (5, 10 and $15{\mu}M$) stimulated (p<0.05) the proliferation of 3T3-L1 preadipocytes (Experiment 1). In the differentiating 3T3-L1 cells supplementation of La ($5{\mu}M$ and $10{\mu}M$), Ce ($5{\mu}M$) and the mixture REE ($5{\mu}M$ and $15{\mu}M$) decreased (p<0.05) the concentration of monounsaturated fatty acids (MUFA) per $10^5cells$, while the supplementation of La ($5{\mu}M$), Ce ($5{\mu}M$) and the mixture REE ($15{\mu}M$) increased (p<0.05) the ratio of saturated fatty acids (SFA) to MUFA. These results indicate that the supplementation of REE to the media may affect proliferation, differentiation and lipogenesis rates of 3T3-L1 cells. However, the effect may depend upon the level or type of REE applied.

Effects of L-tryptophan, Fructan, and Casein on Reducing Ammonia, Hydrogen Sulfide, and Skatole in Fermented Swine Manure

Sheng, Q.K.,Yang, Z.J.,Zhao, H.B.,Wang, X.L.,Guo, J.F. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.8

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide ($H_2S$), and 3-methylindole (skatole). Eighty swine ($50.0{\pm}0.5kg$) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a $3{\times}3$ orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, $H_2S$, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, $H_2S$, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced $H_2S$ production (r = 0.9981). Fructan and Bs significantly interacted in $H_2S$ production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and $H_2S$.

Effects of L-tryptophan, Fructan, and Casein on Reducing Ammonia, Hydrogen Sulfide, and Skatole in Fermented Swine Manure

Q.K. Sheng,Z.J. Yang,H.B. Zhao,X.L. Wang,J.F. Guo 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.8

The effects of daily dietary Bacillus subtilis (Bs), and adding L-tryptophan, fructan, or casein to fecal fermentation broths were investigated as means to reduce the production of noxious gas during manure fermentation caused by ammonia, hydrogen sulfide (H2S), and 3-methylindole (skatole). Eighty swine (50.0±0.5 kg) were equally apportioned to an experimental group given Bs in daily feed, or a control group without Bs. After 6 weeks, fresh manure was collected from both groups for fermentation studies using a 3×3 orthogonal array, in which tryptophan, casein, and fructan were added at various concentrations. After fermentation, the ammonia, H2S, L-tryptophan, skatole, and microflora were measured. In both groups, L-tryptophan was the principle additive increasing skatole production, with significant correlation (r = 0.9992). L-tryptophan had no effect on the production of ammonia, H2S, or skatole in animals fed Bs. In both groups, fructan was the principle additive that reduced H2S production (r = 0.9981). Fructan and Bs significantly interacted in H2S production (p = 0.014). Casein was the principle additive affecting the concentration of ammonia, only in the control group. Casein and Bs significantly interacted in ammonia production (p = 0.039). The predominant bacteria were Bacillus spp. CWBI B1434 (26%) in the control group, and Streptococcus alactolyticus AF201899 (36%) in the experimental group. In summary, daily dietary Bs reduced ammonia production during fecal fermentation. Lessening L-tryptophan and increasing fructan in the fermentation broth reduced skatole and H2S.

Heat Shock Protein Augmentation of Angelica gigas Nakai Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes

Lumbera, Wenchie Marie L.,Cruz, Joseph dela,Yang, Seung-Hak,Hwang, Seong Gu Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.3

There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation through biochemical responses. Previous study showed that Angelica gigas Nakai (AGN) root extract promoted adipogenic differentiation in murine 3T3-L1 preadipocytes under the normal temperature condition. However, its effect in heat shocked 3T3-L1 cells has not been established. In this study, we investigated the effect of AGN root hot water extract in the adipogenic differentiation of murine 3T3-L1 preadipocytes following heat shock and its possible mechanism of action. Thermal stress procedure was executed within the same stage of preadipocyte confluence (G0) through incubation at $42^{\circ}C$ for one hour and then allowed to recover at normal incubation temperature of $37^{\circ}C$ for another hour before AGN treatment for both cell viability assay and Oil Red O. Cell viability assay showed that AGN was able to dose dependently (0 to $400{\mu}g/mL$) increase cell proliferation under normal incubation temperature and also was able to prevent cytotoxicity due to heat shock accompanied by cell proliferation. Confluent preadipocytes were subjected into heat shock procedure, recovery and then AGN treatment prior to stimulation with the differentiation solution. Heat shocked preadipocytes exhibited reduced differentiation as supported by decreased amount of lipid accumulation in Oil Red O staining and triglyceride measurement. However, those heat shocked preadipocytes that then were given AGN extract showed a dose dependent increase in lipid accumulation as shown by both evaluation procedures. In line with these results, real-time polymerase chain reaction (RT-PCR) and Western blot analysis showed that AGN increased adipogenic differentiation by upregulating heat shock protection related genes and proteins together with the adipogenic markers. These findings imply the potential of AGN in heat shock amelioration among 3T3-L1 preadipocytes through heat shock factor and proteins augmentation and enhanced adipogenic marker expression.

Dipyrenylcalix[4]arene—A Fluorescence-Based Chemosensor for Trinitroaromatic Explosives

Lee, Young Hoon,Liu, Hongguang,Lee, Jin Yong,Kim, Sang Hoon,Kim, Sung Kuk,Sessler, Jonathan L.,Kim, Yang,Kim, Jong Seung WILEY-VCH Verlag 2010 Chemistry Vol.16 No.20

<P>A new chemosensor-based approach to the detection of nitroaromatics is described. It involves the analyte-induced quenching of excimer emission of a dipyrenyl calix[4]arene (L). The chemical and photophysical properties of the complexes formed between L and mono-, di-, and trinitrobenzene, and di- and trinitrotoluene were studied in acetonitrile and chloroform by using <SUP>1</SUP>H NMR, UV/Vis, and fluorescence spectroscopy. Fluorescence spectroscopy revealed that the trinitroaromatics engendered the largest response among the various substrates tested, with the sensitivity for these analytes being correspondingly high. Quantitative analysis of the fluorescence titration profile generated from the titration of L with TNT provided evidence that this particular functionalized calix[4]arene receptor allows for the detection of TNT down to the low ppb level in CH<SUB>3</SUB>CN. A single-crystal X-ray diffraction analysis revealed that in the solid state the complex L⋅TNT consists of a supramolecular crystalline polymeric structure, the formation of which appears to be driven by intermolecular π–π interactions between two pyrene units and a TNT molecule held at a distance of 3.2–3.6 Å, as well as by intra- and intermolecular hydrogen-bonds among the amide linkages. Nevertheless, the changes in the <SUP>1</SUP>H NMR, UV/Vis, and fluorescence spectrum, including sharp color changes, are ascribed to a charge-transfer interaction arising from complementary π–π overlap between the pyrene subunits and the bound trinitroaromatic substrates. A number of ab initio calculations were also carried out and, considered in concert, they provide further support for the proposed charge-transfer interactions, particularly in the case of L⋅TNT.</P> <B>Graphic Abstract</B> <P>How to find TNT! A new chemosensor-based approach for the detection of nitrated aromatics is described. It involves the analyte-induced quenching of excimer emission of a dipyrenyl-calix[4]-arene (L; see graphic). In the solid state, the complex L⋅TNT consists of a supramolecular crystalline polymeric structure, the formation of which appears to be driven by intermolecular π–π interactions between two pyrene units and a TNT molecule held at a distance of 3.2–3.6 Å. <img src='wiley_img_2010/09476539-2010-16-20-CHEM200903439-content.gif' alt='wiley_img_2010/09476539-2010-16-20-CHEM200903439-content'> </P>

Pressure-induced dramatic changes in organic–inorganic halide perovskites

Lü,, Xujie,Yang, Wenge,Jia, Quanxi,Xu, Hongwu Royal Society of Chemistry 2017 Chemical Science Vol.8 No.10

<▼1><P>We summarise cutting-edge discoveries and provide insights into the important theme of halide perovskites using pressure as a tuning tool.</P></▼1><▼2><P>Organic–inorganic halide perovskites have emerged as a promising family of functional materials for advanced photovoltaic and optoelectronic applications with high performances and low costs. Various chemical methods and processing approaches have been employed to modify the compositions, structures, morphologies, and electronic properties of hybrid perovskites. However, challenges still remain in terms of their stability, the use of environmentally unfriendly chemicals, and the lack of an insightful understanding into structure–property relationships. Alternatively, pressure, a fundamental thermodynamic parameter that can significantly alter the atomic and electronic structures of functional materials, has been widely utilized to further our understanding of structure–property relationships, and also to enable emergent or enhanced properties of given materials. In this perspective, we describe the recent progress of high-pressure research on hybrid perovskites, particularly regarding pressure-induced novel phenomena and pressure-enhanced properties. We discuss the effect of pressure on structures and properties, their relationships and the underlying mechanisms. Finally, we give an outlook on future research avenues in which high pressure and related alternative methods such as chemical tailoring and interfacial engineering may lead to novel hybrid perovskites uniquely suited for high-performance energy applications.</P></▼2>

The Effect of Trifluoroethanol on Tyrosinase Activity and Conformation: Inhibition Kinetics and Computational Simulations

L체, Z. R.,Shi, L.,Wang, J.,Park, D.,Bhak, J.,Yang, J. M.,Park, Y. D.,Zhou, H. W.,Zou, F. HUMANA PRESS INC 2010 Applied biochemistry and biotechnology Vol.160 No.7

A Survey of Old-field Herbs for Susceptibility to Phenolic Compounds

Stowe, L. Gordon,Kil, Bong-Seop,Yim, Yang-Jai 圓光大學校 基礎自然科學硏究所 1988 基礎科學硏究誌 Vol.7 No.3

北美 Massachusetts주에 있는 묵밭에 나는 名 屬의 草本植物 27種을 골라 實驗室에서 5 x 10^3M ∼ 10^5M 사이의 濃度別 p-coumaric acid와 p-hydroxybenzoec acid가 名 植物의 發芽와 生長에 미치는 影響을 實驗하였다. 그 中 實驗에 성공한 18種에서 얻은 結果로부터 5 x 10^-5M 濃度에서는 대부분의 植物이 對照區와 有意한 差異를 나타내지 않았으나 5 x 10^3M 濃度에서는 심한 抑制를 나타냈으며, 5 x 10^4M 과 5 x 10^3M 사이에 臨界濃度가 있음을 밝혔다. 또 이러한 現象은 p-hydroxybenzoic acid보다 p-coumaric acid에서 더 뚜렷하였다. 發芽, 伸張 및 乾量生長에 있어 대체로 遷移의 初期段階에 나는 種인 Cirsium과 Lepidium 등이 後期段階에 나는 植物種보다 더욱 뚜렷하게 抑制됨으로써 前者의 種間競爭에 중요한 役割을 하는 것으로 해석된다. 또 抵抗指數(resistance indes, 5x 10^3M의 phenolics에 대한 種의 感應)는 種에 따라 달랐지만 發芽率, 伸張, 乾物生長에서 얻은 指數値 사이에는 直線的 相關이 있었다. Phenolic compounds, p-coumaric and p-hydroxybenzoic acids, known as inhibitors for development and growth of many pioneer species on early stage of succession were used for the test of susceptibility in various herbs collected from abandoned agricultural fields in the vicinity of Amherst (U. S. A). The percent inhibition was generally greater for p-coumaric acid than for p-hydroxybenzoic acid. Concentrations of 5x 10^-5 and 5x 10^-4 M generally had no significant effects, but at 5x 10^-3 M was inhibitory to germination and growth of tested species. And the percent inhibition caused by the two phenolics was correlated (r=.843, p<.01). Also the indices of resistance for germination and elongation were significant (r=.695, p<.01) in this study. While cirsium and Lepidium invading species of early stage of succession were sharply susceptible for toxic activity by phenolic acids.

Biogenic reductive preparation of magnetic inverse spinel iron oxide nanoparticles for the adsorption removal of heavy metals

Lingamdinne, L.P.,Chang, Y.Y.,Yang, J.K.,Singh, J.,Choi, E.H.,Shiratani, M.,Koduru, J.R.,Attri, P. Elsevier 2017 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.307 No.-

The accumulation of heavy metal ions in living cells impairs the organ function of living creatures. So, needs to develop new materials with high efficiency of heavy metals removal from the environment. As part of this effort, here, synthesized magnetic inverse spinel iron oxide nanoparticles (MISFNPs) using a biogenic methodology. To synthesize the biogenic MISFNPs, we used the seed extract of Cnidiummonnieri (L.) Cuss (CLC) as a precursor. The prepared MISFNPs was characterized using PXRD, FT-IR, SEM-EDX, SEM, BET, AFM and XPS. We also investigated the size, surface area, structure and magnetic properties of MISFNPs. Additionally, magnetic property measurement system (MPMS) studies show that our prepared MISFNPs was superparamagnetic at room temperature. Further, we used the MISFNPs for the removal of Pb(II) and Cr(III) from aqueous solutions through batch studies. Batch adsorption studies revealed that Pb(II) and Cr(III) follow pseudo-second-order kinetics during adsorption onto the homogenous surface of MISFNPs. Besides, we also found that the adsorption of Pb(II) and Cr(III) on nanoparticles followed an endothermic process. Lastly, we concluded that MISFNPs synthesized by a green route is capable of recycling and removal of heavy metals without loss of its stability.