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Investigation of a Plasma-Etching Process That Uses a Porous Dielectric Template
Xiaoxia Zhong,Luqi Yuan,Xiaochen Wu,Qiwei Shu,Yuxing Xia 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.4
A plasma etching proces of GaN films that used a porous dielectric template was investigated by using numerical simulations. The electrical field, caused by both the substrate bias and the charges acumulated on the insulating template surface, was asumed to affect ions trajectories. Reactive ions enter the sheath with a velocity calculated by using a maxwelian distribution. We found that the microscopic electrical field over the template could change the ion movement. The substrate potential and the nanopore structure showed an ability to affect the etching rate. A peak value of the reactive etching species could be found on the lateral wal in the nanopores. Changing the parameters helped to weaken the peak and, hence, to make the etching proces more effective. The work reported here may help people to understand plasma etching using a porous alumina template.
( Xia Zhong ),( Shaomin Yang ),( Xinying Su ),( Xiaoxia Shen ),( Wenzhao ),( Zhi Chan ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.5
Nitrilase is a valuable hydrolase that catalyzes nitriles into carboxylic acid and ammonia. Its applications, however, are severely restricted by the harsh conditions of industrial reaction processes. To solve this problem, a nitrilase from Acidovorax facilis 72W was inserted into an Escherichia coli-Bacillus subtilis shuttle vector for spore surface display. Western blot, enzyme activity measurements and flow cytometric analysis results all indicated a successful spore surface display of the CotB-nit fusion protein. In addition, the optimal catalytic pH value and temperature of the displayed nitrilase were determined to be 7.0 and 50°C, respectively. Moreover, results of reusability tests revealed that 64% of the initial activity of the displayed nitrilase was still retained at the 10th cycle. Furthermore, hydrolysis efficiency of upscale production of cyanocarboxylic acid was significantly higher in the displayed nitrilase-treated group than in the free group expressed by E. coli (pET-28a-nit). Generally, the display of A. facilis 72W nitrilase on the spore surface of Bacillus subtilis may be a useful method for immobilization of enzyme and consequent biocatalytic stabilization.
Wie Zhou,Xiaoxia Zhong,Luqi Yaun,Qiwei Shu,Xiaochen Wu,Yuxing Xia 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.5I
Titanium-oxide (TiO2) thin films were deposited on unheated (111)-Si substrates by reactive dc magnetron sputtering with a substrate bias voltage of .100 V. A pure metallic titanium target was sputtered in a mixture of argon and oxygen. The structure and the optical properties of the deposited films were studied by changing the value of the total sputtering pressure. X-ray diffraction patterns show the following features: (a) only one wide rutile (110) diffraction peak was observed at a total sputtering pressure of 0.3 Pa; (b) no diffraction peak was observed at 1.2 Pa, implying an amorphous phase; (c) a TiO2 film with a pure anatase phase was obtained at 3.6 Pa, and the dominant diffraction was from the anatase (101) planes. The surface morphology was characterized with atomic force microscopy, and the roughness of the surface was found to increase with the crystal size derived from the XRD data. The optical constants were determined by using spectroscopic ellipsometry. The film deposited at 0.3 Pa, a rutile phase, possessed both a high refractive index and a low extinction coefficient. The value of the optical bandgap for the deposited on films in this experiment was much higher than the reported experimental and theoretical values.D
Hui Jin,Haiyan Cui,Xiaoyan Yang,Lihong Xu,Xudong Li,Rentao Liu,Zhiqiang Yan,Xiuzhuang Li,Weili Zheng,Yuhui Zhao,Xiaoxia Song,Lihua Zhong,Anxiang Su,Bo Qin 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.4
Aphelenchoides besseyi and Ditylenchus destructor can cause serious problems for a number of important agricultural crops and vegetables. In this study, the ethanol extract of Stellera chamaejasme L. roots showed strong nematicidal activity against Aphelenchoides besseyi and Ditylenchus destructor. By using a bioactivity-driven fractionation, three biflavonoids were isolated from the extract and their structures were identified by mass and nuclear magnetic resonance spectral data. Nematicidal activity bioassays revealed that isoneochamaejasmin A had the strongest nematicidal activity against A. besseyi and D. destructor with LC 50 values of 2.32 and 0.18 mM at 72 h, respectively. Chamaejasmenin B displayed weaker nematicidal activity against A. besseyi with an LC 50 value of 3.94 mM at 72 h. Neochamaejasmin B induced the lowest mortality against D. destructor with an LC 50 values of 15.6 mM at 72 h. These results suggested that the kind and position of substitutions and the relative configuration of 2-H/3-H and 2”-H/3”-H could be considered as important factors responsible for the nematicidal activity of these purified C-3/C-3″ biflavonoids.