단감의 이용성 증대를 위한 protopectinase처리 가공기술 개발

하정욱,이대희,김기운,황용일 慶南大學校 附設 基礎科學硏究所 2002 硏究論文集 Vol.16 No.-

가공식품의 개발에 있어서 식품의 맛과 더불어 저장성, 열 안정성 및 색조유지는 소비자의 기호도에 중요한 영향을 미친다. B. subtilis EK11 유래의 protopectinase(PPase)는 식물조직 중엽부의 주성분인 불용성 protopectin을 분해하여 단세포화하는 효소이다. PPase를 단감에 작용시켜 단감 고유의 세포 속에 함유되어 있는 세포내 성분들의 파손 없이 단세포를 유리하였다. PPase처리된 단감 단세포화물의 착즙 후 회수율과 잔사율은 각각 95%와 5%로서, 기계적 마쇄물에서의 85%와 15%에 비하여 높은 회수율과 낮은 잔사율을 나타내었다. 총당, 환원당, 자당, 조단백질, 조지방 및 조섬유의 함량변화는 큰 차이가 없었으며, 이는 단세포 처리에 의하여 이들 성분이 안정하게 유지됨을 알 수 있었다. 식품중 열 또는 빛에 가장 불안정한 비타민 C의 경우 단감 단세포화물은 1일 경과 후에도 50% 이상이 보존되는 것으로 보아 단세포에 의한 일반적인 구성성분이 안정하게 유지 보호됨을 알 수 있다. PPase로 처리된 단감 단세포화물을 4℃에서 9일간 저장하며 색조를 관찰한 결과, 단세포화물에서는 뚜렷한 색조의 차이가 없었고 기계적 마쇄물에서는 변색이 일어났다. 또한 단감 단세포화물을 100℃에서 60분간 열처리한 후 관찰한 결과, 기계적 마쇄물의 경우 짧은 처리에도 변화를 보였으나 단세포화물에서는 그다지 큰 변화가 없었으며 이는 효소적 단세포화물의 높은 열 안정성을 의미한다. PPase를 이용한 단감의 단세포화는 음료제조 및 원료보존에 유용하게 응용가능하며, 나아가 단감 단세포화물의 폭넓은 식품소재화 가능성과 고부가가치 기능성 식품제조에 이용될 수 있음을 의미한다. In development of the processed food, it is important not only to make the food delicious but to enhance its storage span and thermal stability without change of the food quality in color, which greatly affects the tastes of customers. Protopectinase (PPase) from Bacillus subtilis EK11 hydrolyses or dissolves protopectin in the middle lamella of plant tissues with the resultant separation of plant cells from each other, called enzymatic maceration. With the PPase, persimmon was enzymatically macerated to separate cells to primary cell wall without damage. Yields of persimmon treated with PPase and mechanical maceration were 95% and 85%, respectively. Total and reducing sugars, crude protein and fat in the enzymatic maceration were well preserved as in the mechanical maceration. Importantly, over 50% of vitamin C, which is the most unstable component in application of the mechanical maceration, remained with intact form for one day after the enzymatic treatment. When the suspensions of persimmon macerated with both treatments had been stored at 4℃ for 9 days, the suspension of mechanically macerated persimmon was decolorized. Whereas decolorization was not found in the enzymatically macerated persimmon. Moreover, the mechanically macerated persimmon was greatly deteriorated after heat treatment at 100℃ for 60 min; the cell suspension of the enzymatically separated persimmon appeared to be stable, indicating the thermal stability. Thus, the PPase treatment of persimmon could be a better choice for preparation of the highly valuable and functional processed food as well as for prolonging the preservation period.

Odd-Even sorting network을 사용한 non-blocking ATM switch

채일호,김운식,황영원,김상하 충남대학교 기초과학연구소 1994 忠南科學硏究誌 Vol.21 No.1

광대역 통신망의 발달로 ATM switch의 중요성은 점차 증가되고 있으며 이에 대한 많은 연구가 진행되고 있다. ATM switch는 크게 blocking, non-blocking으로 분류된다. 이 중 우리가 관심을 갖는 non-blocking switch는 내부에 buffer를 두거나 Sort-Banyan network을 사용해서 구현된다. 본 논문에서는 Odd-Even Sort를 Sort-Banyan network의 전위 network으로 갖는 ATM switch를 제안한다. 이러한 switch는 기존의 Sort-Banyan switch에 비해서 비교기의 숫자를 감소시킬 수 있어 VLSI구현시 큰 장점을 갖는다.

자연정화공법에 의한 소형 하수처리장치 개발을 위한 최적 깊이 및 호기ㆍ협기 비율

서동철,박미령,곽내운,황하나,이홍재,조주식,허종수 한국환경농학회 2006 한국환경농학회지 Vol.25 No.1

현미 내 철분고함유 벼 종자 간이 선발법

Woon-Ha Hwang,Jung-Sung Back,Sung-Hyun An,Jae-Heok Jeong,Han-Yong Jeong,Hyen-Seok Lee,Jong-Tak Yun,Gun-Hwi Lee,Kyung-Jin Choi 한국국제농업개발학회 2016 韓國國際農業開發學會誌 Vol.28 No.4

We investigate the change of leaf chlorophyll content according to iron content in brown rice when cultivated on the iron limited solid MS medium. By cultivating wild-type and transgenic brown rice in a solid MS medium, we confirmed that iron deficiency chlorosis did not occur in MS media which were contained over 20% of iron content compared to normal MS condition. After selecting twenty kinds of Korean rice varieties, those brown rice were cultivated in solid MS media which were contained from 0 to 15% of iron content compared to normal MS condition then the leaf chlorophyll content was measured. The leaf chlorophyll content was changed according to iron content in brown rice when cultivated in solid MS medium which was contained 0 and 5% of iron content,. There was a strong correlation between iron content in brown rice and leaf chlorophyll content cultivated in solid MS medium with 5% of Fe content. Therefore we expect that analysis of leaf chlorophyll content after cultivated on MS medium with 5% iron content compared to normal MS media condition will be more simple and effective method to screening high iron content brown rice without measurement of iron content.

The Simple and Rough Screening Method of Phosphorus Deficient Tolerance Rice

Woon-Ha Hwang,Dae-Wook Kim,Jae-Heok Jeong,Han-Yong Jeong,Hyen-Seok Lee,Kyung-Jin Choi,Gun-Hwi Lee,In-Jung Lee,Sung-Hwan Oh 한국작물학회 2015 Korean journal of crop science Vol.60 No.4

Even though phosphorus (P) is essential element for plant growth and development, it is not enough for crop production in soil. To breed more P deficient tolerance rice, screening and selection in rice population is needed. We tried to develop more simple and rough screening method for breeding of P deficient tolerance rice. In P deficient condition, tiller number was dramatically decreased among yield components in rice. Though this result, we confirmed tiller number could be the best marker in screening of P deficient tolerance rice. 480 rice genetic resources were cultivated in rice bed tray filled with P deficient soil for four weeks and each dry weight was measured. Among them, the 55 kinds of genetic resource were selected then cultivated in paddy field with 3 fertilizer conditions. Plant dry weight and tiller number in ripening stage were shown significant difference according to P condition. Plant dry weight and tiller number in ripening stage was highly correlated especially in P deficient condition. Furthermore, the tiller number in ripening stage and plant dry weight in rough screening were shown high degree correlation. Though these results, we might expect measuring of plant dry weight after cultivation in rice bed tray filled with P deficient soil could be a simple and effective screening method in selection of P deficient tolerance rice.

Selection of the Korean rice cultivars having adaptable heading characters in tropical regions

Hwang Woon-Ha,Ahn Seung-Hyeon,Jeong Jae-Heok,Jeong Han-Yong,Lee Hyen-Seok,Yoon Jong-Tak,Lee Gun-Hwi,Choi Kyung-Jin 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-

The effective method to screen high Fe content brown rice

Woon-Ha Hwang,Soo-Kwon Park,Dongjin Shin,Min-Hee Nam,In-Jung Lee,Dong-Soo Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Even rice is one of the most important food crops in the world, its micronutrient contents including iron is not enough to solve mineral malnutrition which is a significant public health issue in most developing countries. Iron deficiency is probably the most widespread micronutrient deficiency in humans. Experts estimate that a rice based diet should contain 14.5 ppm iron in endosperm. However, Cesar P et al reported that average iron content in milled rice was 2 ~ 3 ppm, whease it was 10 ~ 11 ppm in brown rice. Fe content of rice is usually measured by inductively plasma spectrometry (ICP). It takes times and could make error while sample processing. To breed high iron contained rice variety, the effective screen method for select high iron contained elite line is essential. To develop more effective method in screening high Fe contained brown rice, we investigate the relation the leaf chlorophyll content with iron content in brown rice. Result of analyzing leaf chlorophyll content of OsNAS3-OX which contain more Fe than wild-type plant after cultivated on Fe limited MS medium, those of OsNAS3-OX was higher than those of wild-type plant in 0 and 20 % Fe contained MS medium. After measured Fe content in twenty kinds of brown rice, we cultivated those in Fe limited MS medium then investigate the relation of leaf chlorophyll content with Fe content of brown rice. In 0 and 5 % Fe contained MS medium, the leaf chlorophyll content was highly related with Fe content of brown rice as 0.66 and 0.79. Though these result, analyze of leaf chlorophyll content cultivated in 5 % Fe content in MS media was effect on screening high Fe contained.

Ripening Characteristics and Antioxidant Enzyme Activity of Ilmi under Heat Stress Condition

Hwang, Woon-Ha,Jeong, Jae-Hyeok,Lee, Hyeon-Seok,Choi, In-Bae,Ahn, Seung-Hyeon,Jeong, Han-Young,Yoon, Jong-Tak,Choi, Kyung-Jin The Korean Society of Crop Science 2018 한국작물학회지 Vol.63 No.2

Heat stress during the ripening stage reduces the yield and quality of rice. Considering the adverse effects of global warming, it is necessary to breed heat stress tolerant rice cultivars and analyze their stress tolerance characteristics. We investigated the ripening characteristics and antioxidant enzyme activity of Ilmi under heat stress condition during the ripening stage. Ripening rate, 1000 brown grain weight, and rice quality of Ilmi were not significantly changed by heat stress during the ripening stage. Leaf chlorophyll, chlorophyll a/b ratio, and malondialdehyde (MDA) contents of Ilmi leaves were also less changed than those of Ilpum. These results strongly suggested that Ilmi has heat tolerance characteristics during the ripening stage. Analysis of antioxidant enzyme activity of Ilmi revealed that peroxidase (POX) activity was significantly higher than that of Ilpum, and showed a significant correlation with the change in chlorophyll a/b ratio and hydrogen peroxide content of flag leaves of Ilmi. These data suggest that the high POX enzyme activity of Ilmi could be considered one of its major heat tolerance characteristics.

The Change of Agronomic Characters and Pi-deficient Stress Resistance by Over-expression OsPT6 in Rice

( Woon Ha Hwang ),( Soo Kwon Park ),( Dong Jin Shin ),( Min Hee Nam ),( Do Hoon Kim ),( In Jung Lee ),( Dong Soo Park ) 한국국제농업개발학회 2014 韓國國際農業開發學會誌 Vol.26 No.2

We investigated the change of growth characters by over-expressing OsPT6 (OsPT6-OX) under phosphorus (Pi) deficient condition in rice. In analyzing the change of growth rate according to Pi treatment for two weeks, shoot growth rate of OsPT6-OX was increased in Pi deficient condition than in Pi sufficient condition. Therefore, the chlorophyll content of leaf in OsPT6-OXs did not increase even decreased in the youngest leaf. However growth rate of wild type plant in Pi deficient condition showed significant reduction with increment of chlorophyll content. In analyzing agronomic characters, the tiller number of OsPT6-OX less decreased than those of wild type plant in Pi deficient condition. Panicle number and ripening rate of wild type plant were changed according to Pi treatment. However those of OsPT6-OX did not change in such conditions. Resulting of changes in agronomic characters, yield of wild type plant decreased about 10 % in Pi deficient condition. That of OsPT6-OX did not show significant difference in Pi deficient condition. The results obtained this study demonstrated that over-expression of OsPT6 could increase phosphorus deficient stress resistance in rice.

Phosphate uptake and growth characteristics of transgenic rice under high phosphate soils

Woon-Ha Hwang,Soo-Kwon Park,Tackmin Kwon,Sais-Beul Lee,Min-Hee Nam,Doh-Hoon Kim,Dong-Soo Park 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

Farmers have use phosphate fertilizer to provide sufficient yields. However, overuse of phosphorus accumulate in soil and causes soil and water pollution. We evaluated the phosphate acquisition and growth characteristics of OsPT1 transgenic rice (OsPT1-OX, over-expressing the high affinity phosphate transporter 1) in high phosphate soils with different level of nitrogen fertilizer treatment to investigate removing ability of excessive phosphate from soil. OsPT1-OX had shorter culm length but more tillers than those of wild-type plants in each soil conditions. Phosphate content per dry weight of OsPT1-OX was 1.8 times higher than that of wild-type under control fertilizer treated conditions. Although the dry weight of OsPT1-OX was not different from that of wild-type plants, whole plant phosphate content was 1.7 times higher than that of wild-type plants under control fertilizer conditions. Tiller number and phosphate content per dry weight of wild-type plants increased following high levels of phosphate application but did not change by following additional nitrogen application. Tiller number and phosphate content per dry weight of OsPT1-OX did not change under the high phosphate condition, but increased following nitrogen application under similar conditions. Whole plant phosphate content was highest under high nitrogen and high phosphate application conditions. These results suggest that OsPT1-OX may reduce phosphate content in soils containing excess phosphate and may be further effective under high nitrogen condition.