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RISS 인기검색어
- 검색키워드
- 저자 : William J . Kelleher Arthur E . Schwarting ( Byong Kak Kim ) (검색결과 2 건)
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Claviceps paspali 의 액내배양에서의 라이써직산 알카로이드 생성에 대한 비산의 촉진작용
김병각,William J . Kelleher Arthur E . Schwarting ( Byong Kak Kim ) 생화학분자생물학회 1972 BMB Reports Vol.5 No.1
Analysis of the culture filtrates of lysergic acid alkaloid-producing fermentations at intervals during growth of the cultures showed that phosphate is most rapidly depleted from the medium in fermentations that produce the highest levels of alkaloid. In view of the known interference with phosphate metabolism by arsenate, sodium arsenate was added to the culture medium with the intent of creating, in effect, a phosphate deficiency. The addition of arsenate at levels between one-fiftieth and one-twentieth the molar concentration of phosphate resulted in increases in alkaloid production up to 100 per cent. Experiments with ten-times the usual concentration of phosphate showed that the ratio of arsenate to phosphate rather than the absolute concentration of arsenate was most significant in obtaining this effect. Increasing the concentration of phosphate alone (up to twenty times the usual concentration) resulted in substantial increases in alkaloid production. The effect of arsenate on both the rate and the extent of phosphate uptake was determined. Among several inhibitors of electron transport and/or oxidative phosphorylation, only the uncoupler of oxidative phosphorylation (dinitrophenol) was effective in increasing alkaloid production.
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김병국,Kim, Byong-Kak,Kelleher, William J.,Schwarting, Arthur E. 생화학분자생물학회 1972 한국생화학회지 Vol.5 No.1
라이써직산 알카로이드를 생성하는 발효에서 맥각균 Claviceps paspali의 성장과정중 경시적으로 발효액을 분석하여 본바, 가장 높은 농도의 알카로이드를 생성하는 발효액에서 인산이 가장 신속히 소모됨을 알았다. 인산대사가 비산에 의해 방해된다는 점에 착안하여 일종의 인산결핍상태를 만들 목적으로 배양액에다 비산나트륨을 첨가하였다. 인산의 0.02 및 0.05 몰 농도에 해당하는 비산을 첨가하였을때 알카로이드 생성이 100% 까지 증가하는 결과를 나타내었다. 폐지의 정상농도의 10배의 인산을 사용한 실험에서, 알카로이드의 생성증가에 중요한 영향을 미치는 것은 비산의 절대농도가 아니라 인산 대 비산의 비 임을 알게 되었다. 인산 농도만을 증가시켰을 때(정상 농도의 20배까지), 알카로이드 생성이 상당히 증가되는 결과를 초래하였다. 맥각균의 인산 섭취의 범위와 속도에 대한 비산의 영향을 측정하였다. 전자 수송 혹은 산화적 인산화 또는 두 가지 모두를 저지하는 수종의 억제제 중에서 다이나이트로훼놀만이 알카로이드 생성을 촉진시키는 데 유효하였다. Analysis of the culture filtrates of lysergic acid alkaloid-producing fermentations at intervals during growth of the cultures showed that phosphate is most rapidly depleted from the medium in fermentations that produce the highest levels of alkaloid. In view of the known interference with phosphate metabolism by arsenate, sodium arsenate was added to the culture medium with the intent of creating, in effect, a phosphate deficiency. The addition of arsenate at levels between one-fiftieth and one-twentieth the molar concentration of phosphate resulted in increases in alkaloid production up to 100 per cent. Experiments with ten-times the usual concentration of phosphate showed that the ratio of arsenate to phosphate rather than the absolute concentration of arsenate was most significant in obtaining this effect. Increasing the concentration of phosphate alone (up to twenty times the usual concentration) resulted in substantial increases in alkaloid production. The effect of arsenate on both the rate and the extent of phosphate uptake was determined. Among several inhibitors of electron transport and/or oxidative phosphorylation, only the un coupler of oxidative phosphorylation (dinitrophenol) was effective in increasing alkaloid production.
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