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Poria cocos ethanol extract enhances pentobarbital-induced sleeping behaviors in mice agonist
Vikash Kumar Shah, 권영옥, 이미경, 오기완 충북대학교 약품자원개발연구소 2014 약학논문집 Vol.29 No.-
The treatment of insomnia requires evidence-based effective pharmacological treatments. The present study investigated whether Poria cocos ethanol extract (PCE) enhances pentobarbital- induced sleeping behaviors in mice. Our result showed that PCE inhibited locomotor activity in mice. PCE potentiated pentobarbital-induced sleep time, and shortend sleep latency at the hypnotic (40mg/kg) dose of pentobarbital. The extract also increased the number of sleeping mice and total sleeping time, showing synergistic effects, similar to muscimol (a GABAA agonist, 0.2 mg/kg) at the sub-hypnotic (28 mg/kg) dosage in mice. The findings provide experimental evidence for the potential use of PCE in insomnia treatment.
Pachymic Acid Enhances Pentobarbital-Induced Sleeping Behaviors via GABAA-ergic Systems in Mice
( Vikash Kumar Shah ),( Jae Joon Choi ),( Jin Yi Han ),( Mi Kyeong Lee ),( Jin Tae Hong ),( Ki Wan Oh ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.4
This study was investigated to know whether pachymic acid (PA), one of the predominant triterpenoids in Poria cocos (Hoelen) has the sedative-hypnotic effects, and underlying mechanisms are mediated via γ-aminobutyric acid (GABA)-ergic systems. Oral administration of PA markedly suppressed locomotion activity in mice. This compound also prolonged sleeping time, and reduced sleep latency showing synergic effects with muscimol (0.2 mg/kg) in shortening sleep onset and enhancing sleep time induced by pentobarbital, both at the hypnotic (40 mg/kg) and sub-hypnotic (28 mg/kg) doses. Additionally, PA elevated intracellular chloride levels in hypothalamic primary cultured neuronal cells of rats. Moreover, Western blotting quantitative results showed that PA increased the amount of protein level expression of GAD65/67 over a broader range of doses. PA increased α- and β-subunits protein levels, but decreased γ-subunit protein levels in GABAA receptors. The present experiment provides evidence for the hypnotic effects as PA enhanced pentobarbital-induced sleeping behaviors via GABAA-ergic mechanisms in rodents. Taken together, it is proposed that PA may be useful for the treatment of sleep disturbed subjects with insomnia.
Vikash Kumar Shah,Sam-Shik Na,Myong-Soo Chong,Jae-Hoon Woo,Yeong-Ok Kwon,Mi Kyeong Lee,Ki-Wan Oh 충북대학교 동물의학연구소 2015 Journal of Biomedical and Translational Research Vol.16 No.3
Poria cocos is a well-known traditional Chinese traditional medicine (TCM) that grows around roots of pine trees in China, Korea, Japan, and North America. Poria cocos has been used in Asian countries to treat insomnia as either a single herb or part of an herbal formula. In a previous experiment, pachymic acid (PA), an active constituent of Poria cocos ethanol extract (PCE), increased pentobarbital-induced sleeping behaviors. The aim of this experiment was to evaluate whether or not PCE and PA modulate sleep architectures in rats as well as whether or not their effects are mediated through GABAA-ergic transmission. PCE and PA were orally administered to individual rats 7 days after surgical implantation of a transmitter, and sleep architectures were recorded by Telemetric Cortical encephalogram (EEG) upon oral administration of test drugs. PCE and PA increased total sleep time and non-rapid eye movement (NREM) sleep as well as reduced numbers of sleep/wake cycles recorded by EEG. Furthermore, PCE increased intracellular chloride levels, GAD65/67 protein levels, and α-, β-, and γ-subunits of GABAA receptors in primary cultured hypothalamic neuronal cells. These data suggest that PCE modulates sleep architectures via activation of GABAA-ergic systems. Further, as PA is an active component of PCE, they may have the same pharmacological effects.