Field Evaluation of Mungbean Recombinant Inbred Lines against Mungbean Yellow Mosaic Disease Using New Disease Scale in Thailand

Akhtar, Khalid P.,Kitsanachandee, R.,Srinives, P.,Abbas, G.,Asghar, M.J.,Shah, T.M.,Atta, B.M.,Chatchawankanphanich, O.,Sarwar, G.,Ahmad, M.,Sarwar, N. The Korean Society of Plant Pathology 2009 Plant Pathology Journal Vol.25 No.4

Studies were conducted to identify the sources of resistance in mungbean recombinant inbred lines (RILs) in Thailand against mungbean yellow mosaic disease (MYMD). 146 mungbean RILs in $F_8$ series were evaluated in a field including resistant parent NM-10-12-1 and susceptible parent KPS 2 during summer 2008 under high inoculum pressure. The RILs were subsequently scored for disease symptom severity ratings (DSSR) using a new scale. Observations regarding DSSR and % disease index (%DI) showed that the tested RILs responded differently to the disease. A large number of RILs (132) were found highly susceptible, 12 were susceptible, 3 were tolerant and one was resistant. Overall screening results showed that three RILs, viz. line no. 30, 100 and 101 had minimum DSSR and % disease index thus they are good source of resistance to MYMD in spite of high disease pressure and can therefore be used directly as varieties to manage the disease in Thailand.

Sensitive NADH detection in a tumorigenic cell line using a nano-biosensor based on the organic complex formation

Akhtar, M.H.,Mir, T.A.,Gurudatt, N.G.,Chung, S.,Shim, Y.B. Elsevier Applied Science 2016 Biosensors & Bioelectronics Vol.85 No.-

A robust amperometric sensor for β-nicotinamide adenine dinucleotide (NADH) detection was developed through the organic complex formation with ethylenediaminetetraacetic acid (EDTA) bonded on the polyethylenimine (PEI)/activated graphene oxide (AGO) layer. The EDTA immobilized sensor probe (GCE/AGO/PEI-EDTA) revealed a catalytic property towards NADH oxidation that allows for the highly sensitive electrochemical detection of NADH at a low oxidation potential. Surface characterization demonstrated that the negatively charged AGO acted as nanofillers in the positively charged PEI matrix through the charge interaction. The immobilization of EDTA on the polymer layer provided more surface area for NADH to interact with through the enhanced chemical interlocking between them. We observed the strong interaction between NADH and EDTA on the AGO/PEI layer using a quartz crystal microbalance (QCM), X-ray photoelectron spectroscopy (XPS), and the calculation of the minimized energy for complex formation. The dynamic range of NADH was determined to be between 0.05μM and 500μM with a detection limit (LD) of 20.0+/-1.1nM. The reliability of the developed sensor for biomedical applications was examined by detecting NADH in tumorigenic lung epithelial cells using the standard addition method.

Low temperature solution processed Mn₃O₄ nanoparticles: Enhanced performance of electrochemical supercapacitors

Bui, P.T.M.,Song, J.H.,Li, Z.Y.,Akhtar, M.S.,Yang, O.B. Elsevier Sequoia 2017 JOURNAL OF ALLOYS AND COMPOUNDS Vol.694 No.-

Well-crystalline Mn<SUB>3</SUB>O<SUB>4</SUB> nanoparticles (NPs) were synthesized by an economical and high yield facile low temperature solution process at 80 <SUP>o</SUP>C for electro-active electrode materials in supercapacitor applications. The semispherical morphology of Mn<SUB>3</SUB>O<SUB>4</SUB> NPs was observed which have the average particle size of 20-30 nm. The crystalline, structural, compositional and optical properties of synthesized materials evidenced the formation of Mn<SUB>3</SUB>O<SUB>4</SUB> without existing any other oxide phases, possessing the typical tetragonal structure of Mn<SUB>3</SUB>O<SUB>4</SUB> with the band gap of 2.37 eV. To evaluate the properties of the synthesized Mn<SUB>3</SUB>O<SUB>4</SUB> NPs as effective electrode in electrochemical double layer supercapacitors (EDLCs), the cyclicvoltammetry and galvanostatic charge-discharge cycling analysis were carried out in 2 M TEABF<SUB>4</SUB> electrolyte. Mn<SUB>3</SUB>O<SUB>4</SUB> NPs electrode with activated carbon showed the highest capacitance of ~216 Fg<SUP>-1</SUP> as compared to bare AC and bare Mn<SUB>3</SUB>O<SUB>4</SUB> NPs electrodes. An excellent stability by maintaining ~85% of initial value after 1000 cycles was recorded for Mn<SUB>3</SUB>O<SUB>4</SUB> NPs-AC electrode based EDLC.

Field Evaluation of Mungbean Recombinant Inbred Lines against Mungbean Yellow Mosaic Disease Using New Disease Scale in Thailand

Khalid P. Akhtar,R. Kitsanachandee,P. Srinives,G. Abbas,M. J. Asghar,T. M. Shah,B. M. Atta,O. Chatchawankanphanich,G. Sarwar,M. Ahmad,N. Sarwar 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.4

Studies were conducted to identify the sources of resistance in mungbean recombinant inbred lines (RILs) in Thailand against mungbean yellow mosaic disease (MYMD). 146 mungbean RILs in F8 series were evaluated in a field including resistant parent NM-10- 12-1 and susceptible parent KPS 2 during summer 2008 under high inoculum pressure. The RILs were subsequently scored for disease symptom severity ratings (DSSR) using a new scale. Observations regarding DSSR and % disease index (%DI) showed that the tested RILs responded differently to the disease. A large number of RILs (132) were found highly susceptible, 12 were susceptible, 3 were tolerant and one was resistant. Overall screening results showed that three RILs, viz. line no. 30, 100 and 101 had minimum DSSR and % disease index thus they are good source of resistance to MYMD in spite of high disease pressure and can therefore be used directly as varieties to manage the disease in Thailand.

An Overview of Biopulping Research: Discovery and Engineering

Scott, Gary M.,Akhtar, Masood,Lentz, Michael J.,Horn, Eric,Swaney, Ross E.,Kirk, T.Kent Korea Technical Association of the Pulp and Paper 1998 펄프.종이기술 Vol.30 No.4

Biopulping is defined as the treatment of wood chips with lignin-degrading fungi prior to pulping. Fungal pretreatment prior to mechanical pulping reduces electrical energy requirements during refining or increases mill throughput, improves paper strength, reduces the pitch content, and reduces the environmental impact of pulping. Our recent work involved scaling up the biopulping process towards the industrial level, investigating both the engineering and economic feasibility. We envision the process to be done in either a chip-pile or silo-based system for which several factors need to be considered: the degree of decontamination, a hospitable environment for the fungus, and the overall process economics. Currently, treatment of the chips with low-pressure steam is sufficient for decontamination and a simple, forced ventilation system maintains the proper temperature, humidity, and moisture conditions, thus promoting uniform growth of the fungus. The pilot-scale trial resulted in the successful treatment of 4 tons of wood chips (dry weight basis) with results comparable to those on a laboratory. Larger, 40-ton trials were also successful, with energy savings and paper properties comparable with the laboratory scale. The overall economics of the process also look very favorable and can result in significant annual savings to the mill. Although the current research has focused on biopulping for mechanical pulping, it is also beneficial for sulfite chemical pulping and some applications to recycled fiber have been investigated.

Low temperature HFCVD synthesis of tungsten oxide thin film for high response hydrogen gas sensor application

Godbole, Rhushikesh,Ameen, Sadia,Nakate, Umesh T.,Shaheer Akhtar, M.,Shin, Hyung-Shik Elsevier 2019 Materials letters Vol.254 No.-

<P><B>Abstract</B></P> <P>A quick and efficient approach was employed for the growth of nanocrystalline tungsten oxide (WO<SUB>3</SUB>) thin films using one-step hot filament chemical vapor deposition (HFCVD) method at low temperature. In a typical experiment, the parent material tungsten (W) was subjected to oxidation, gasification and its subsequent condensation to obtain the uniform deposition of WO<SUB>3</SUB> thin film on silicon substrate at significantly low temperature of ∼200 °C. Prepared WO<SUB>3</SUB> thin film possessed the cauliflower nanostructure (WCNs) with typical monoclinic WO<SUB>3</SUB> crystal structure. Prepared WCNs thin films were applied for the detection of hydrogen (H<SUB>2</SUB>) gas at 100 ppm. H<SUB>2</SUB> response increased with rise in temperature and the maximum response of ∼87% was obtained at the optimized temperature of ∼250 °C with response time 180 s.</P> <P><B>Highlights</B></P> <P> <UL> <LI> WO<SUB>3</SUB> thin films are obtained through low temperature HFCVD technique. </LI> <LI> WO<SUB>3</SUB> thin films exhibits cauliflower morphology (WCNs) with typical monoclinic WO<SUB>3</SUB> crystal structure. </LI> <LI> Highly uniform WCNs thin film is prepared. </LI> <LI> High sensitivity of ∼87% towards H<SUB>2</SUB> gas is achieved. </LI> </UL> </P>

Identification of QTLs Associated with Cold Tolerance in Rice(O. sativa L.)

You-Chun Song,Jun-Hyeon Cho,Woon-Gu Ha,Ji-Yoon Lee,No-Bong Park,Jin-Il Choung,Do-Yeon Kwak,Min-Hee Nam,Shambhu P. Khatiwada,T. Akhtar,B. Chaudhary,Byeon-Geun Oh 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Cold tolerance at every growing stage of rice(Oryza sativa L.) is one of the main determinations for the stable growth in temperature and high elevate area. In the current study, a 181 lines of BC population derived from a cross of Gayabyeo, a Tongil type sensitive to cold and Chhamrong a tolerant to cold were evaluated for cold tolerance with cold water irrigation(17℃) at seeding stage as well as low temperature germinate at 13℃, respectively. The resulting linkage map consists of 157 marker loci, covering all of 12 rice chromosomes and spanning 910cM(Haldane function) with an average interval of 76cM between markers. Three main-effect QTLs were identified. The comparison of the OTLs identified in this cold treatments resulted in an intriquing finding that each treatments were controlled by a major QTL. The QTL qCWI-4 on chromosome 4 was found to increase its additive effect to -0.84 as the cold water irrigate stress was given. In the meanwhile, the QTL qLTG-8 was detected with a LOD score of5.54, explaining up to 13% of the phenotypic variation controlled by Gayabyeo allele. In addition, the QTL qLTGV-3, controlled by Gayabyeo allele with a LOD score of 5.19 explaining about 12.5% of the variation was also identified. These results would favor our better understanding of the genetic control of cold tolerance in rice and be important for the development of rice cultivars with a broaden climatic adaptation.

Identification of QTLs Associated with Cold Tolerance under High Elevation Rice Growing Area in Rice(O. sativa L.)

You-Chun Song,Jun-Hyeon Cho,Woon-Gu Ha,Ji-Yoon Lee,No-Bong Park,Jin-Il Choung,Shambhu P. Khatiwada,T. Akhtar,B. Chaudhary,Byeon-Geun Oh 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10

Cold sensitivity has been shown to vary and the cold tolerance from vegetative to reproductive stage appears to be independent during the life cycle. In the current study, cold tolerance under high elevation rice growing area were evaluated using F4 generation of 181 lines derived from Gayabyeo*2/Chhamrong. Five main-effect QTLs related to days to heading, panicle exertion and spikelet fertility were identified. The QTL qDH-3 on chromosome 3 showed the peak LOD score of 6.3, explaining up to 16.5% of phenotypic variation with additive effect of -2.6. Moreover, the QTL qPE-3 and qSF-3 on chromosome 3 were coincided with the QTL qDH-3 showing an opposite allele effects. Thus, the region harboring marker RM523-RM14281 could be helpful for the selection of cold tolerance genotypes in marker assisted selection(MAS) of rice breeding program.