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Correlation between in vitro fertilization and artificial insemination in Holstein bulls
Sun Wei,Li Yunxia,Su Jie,Bao Xiangnan,Ding Rui,Zhao Gaoping,Cao Guifang,Hu Shuxiang,Wang Jianguo,Sun Qingyuan,Yu Haiquan,Li Xihe 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12
Objective: Owing to the lack of a breeding index for efficient and quick fertility evaluations of Holstein bulls when using traditional or genome-wide detection methods, this study aimed to determine whether in vitro fertilization (IVF) could be used as an indicator of conception rate of artificial insemination (AI).Methods: Conventional and sexed frozen semen from nine bulls were used for IVF and AI.Results: The IVF and AI conception rates of each bull were confirmed to be positively correlated between the conventional frozen and sexed frozen semen. The correlation coefficient R values of nine bulls between IVF and AI methods were 0.73 and 0.97 for the conventional frozen and sexed frozen semen, respectively. The average conception rate of three bulls undergoing AI was 69.5% and 64.2%, 61.8% and 58.8%, and 48.2% and 46.2% in first-, second-, and third-born cows when conventional frozen and sexed frozen semen were used, respectively, which showed a positive correlation with the fertilization rate in the same parity. We propose an evaluation standard to assess the fertilization ability of bulls based on their IVF test results, which is categorized into three grades: grade one, normal fertility bull with an AI conception rate of 40%±5% and IVF rate of 45% to 60%; grade two, higher fertility bull with an AI conception rate of 50%±5% and IVF rate of 61% to 80%; and grade three, highest fertility bull with an AI conception rate of 60%±5% and IVF rate of >80%.Conclusion: These findings reveal that IVF results can be used as a breeding index for bulls to evaluate their AI conception ability, which may shorten the time required to select bulls for breeding. Objective: Owing to the lack of a breeding index for efficient and quick fertility evaluations of Holstein bulls when using traditional or genome-wide detection methods, this study aimed to determine whether in vitro fertilization (IVF) could be used as an indicator of conception rate of artificial insemination (AI). Methods: Conventional and sexed frozen semen from nine bulls were used for IVF and AI. Results: The IVF and AI conception rates of each bull were confirmed to be positively correlated between the conventional frozen and sexed frozen semen. The correlation coefficient R values of nine bulls between IVF and AI methods were 0.73 and 0.97 for the conventional frozen and sexed frozen semen, respectively. The average conception rate of three bulls undergoing AI was 69.5% and 64.2%, 61.8% and 58.8%, and 48.2% and 46.2% in first-, second-, and third-born cows when conventional frozen and sexed frozen semen were used, respectively, which showed a positive correlation with the fertilization rate in the same parity. We propose an evaluation standard to assess the fertilization ability of bulls based on their IVF test results, which is categorized into three grades: grade one, normal fertility bull with an AI conception rate of 40%±5% and IVF rate of 45% to 60%; grade two, higher fertility bull with an AI conception rate of 50%±5% and IVF rate of 61% to 80%; and grade three, highest fertility bull with an AI conception rate of 60%±5% and IVF rate of >80%. Conclusion: These findings reveal that IVF results can be used as a breeding index for bulls to evaluate their AI conception ability, which may shorten the time required to select bulls for breeding.
Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng
Sun, Hang,Liu, Fangbing,Sun, Liwei,Liu, Jianzeng,Wang, Manying,Chen, Xuenan,Xu, Xiaohao,Ma, Rui,Feng, Kai,Jiang, Rui The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2
Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.
Improved information fusion approach based on D-S evidence theory
Rui Sun,Hong-Zhong Huang,Qiang Miao 대한기계학회 2008 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.22 No.12
Conventional D-S evidence theory has an unavoidable disadvantage in that it will give counter-intuitive result when fusing high conflict information. This paper proposes an improved method to solve this problem. By reassigning weight factors before fusing, the method can give reasonable results especially when the initial weight factors of conflict evidences are almost equal. It gives an adjustable factor to adjust the reassigning force. An example is given to illustrate these advantages.
Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng
Hang Sun,Fangbing Liu,Liwei Sun,Jianzeng Liu,Manying Wang,Xuenan Chen,Xiaohao Xu,Rui Ma,Kai Feng,Rui Jiang 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2
Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.
Avery Rui Sun,Qili Sun,Yansong Wang,Liqiu Hu,Yutong Wu,Fenbo Ma,Jiayi Liu,Xiangchao Pang,Bin Tang 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background Titanium (Ti) is now widely used as implant material due to its excellent mechanical properties and superior biocompatibilities, while its inert bioactivities might lead to insufficient osseointegration, and limit its performance in dental applications. Methods We introduced a robust and simple approach of modifying titanium surfaces with polysaccharide complexes. Titanium samples were subjected to hydrothermal treatment to create a uniform porous structure on the surface, followed by coating with a bioinspired and self-assembly polydopamine layer. Strontium Eucommia Ulmoides Polysaccharide (EUP-Sr) complexes are then introduced to the polydopamine-coated porous titanium. Multiple morphological and physiochemical characterizations are employed for material evaluation, while cell proliferation and gene expression tests using macrophages, primary alveolar bone osteoblasts, and vascular endothelial cells are used to provide an overall insight into the functions of the product. The significances of statistical differences were analyzed using student’s t-test. Results Microscopic and spectrometric characterizations confirmed that the Ti surface formed a porous structure with an adequate amount of EUP-Sr loading. The attachment was attributed to hydrogen bonding between the ubiquitous glycosidic linkage of the polysaccharide complex and the ring structure of polydopamine, yet the loaded EUP-Sr complex can be gradually released, consequently benefiting the neighboring microenvironment. Cell experiments showed no cytotoxicity of the material, and the product showed promising anti-inflammation, osseointegration, and angiogenesis properties, which were further confirmed by in vivo evaluations. Conclusion We believe the EUP-Sr modified titanium implant is a promising candidate to be used in dental applications with notable osteoimmunomodulation and angiogenesis functions. And the novel technique proposed in this study would benefit the modification of metal/inorganic surfaces with polysaccharides for future research.
손예 ( Sun Rui ),홍창기 ( Hong Chang-kee ) 커뮤니케이션디자인학회 2022 커뮤니케이션 디자인학연구 Vol.81 No.-
찐빵은 대한민국의 대표 먹거리이자 전통 간식이다. 가창찐빵거리는 대구광역시에서 청도로 가는 30번국도 변을 중심으로 500여에 걸쳐 형성되어 있으며 10여 곳의 찐빵집이 성업 중이다. 2000년 첫 번째 찐빵집이 들어선 이후, 2002년 대구 지역 방송 에 알려지면서 입소문을 타기 시작하였고, 2003년에는 전국적인 관심을 얻으며 특성화 골목으로 명성을 떨치게 되었다. 찐빵 특화 거리로 대구 시뿐 만 아니라 전국적으로 유명세를 떨쳤던 가 창 찐빵 거리는 현재 상인들이 경기 침체와 매출 감소로 어려움을 겪고 있다. 2019년의 코로나 때문에 상황이 더 설상 가상이다. 본 연구는 이상 실제상황을 반영하여 지역경제 활성화에 대한 지역특화 전략의 중요성이 증대됨에 따라 지역특수성을 강조하여 가창 찐빵 골목의 부진을 진단했다. 플레이스 브랜딩과 복합문화공간에 관한 선행연구 및 이론적 고찰을 통해 플레이스 브랜딩의 3가지 유형인 ‘장소 기반, 콘텐츠 기반, 환경 문화적 기반’을 도출하고 복합문화공간의 4가지 유형인 ‘문화예술 형, 상업 공간 형, 체험 공간 형, 지역 자원융합 형’을 도출하여 종합 디자인과 서비스 디자인 분야에서 전략적 연결점을 찾아 맞춤형 요소를 융합하는 방안을 제시하며, 브랜드공간과 서비스디자인에 있어 새로운 방향과 아이디어와 분석을 제시하고 실사 및 디자인개발을 통해 가창찐빵골목의 재생을 희망하는 목적을 달성하였다. 본 연구를 통해 지역 브랜드 향상을 위한 방법으로 지역자원인 문화와 역사적 건물들을 활용하고 지역주민과의 연계를 통한 프로그램을 계획하여 그 지역만의 상징성을 느낄 수 있는 장소로 변화된다면 이와 동시에 본 연구는 플레이스 브랜드에 대한 이론적 고찰을 통해 지역의 브랜드 파워에 대한 경제활 성화, 지역 특성화 홍보의 효과가 있으며, 새로운 가치 창출을 통한 과정의 창출을 희망하며, 미래의 플레이스 브랜딩 디자인과 마케팅 분야 및 체계적인 창의적 방법의 결과 도출에 의의가 있다. Pastry is a representative food and traditional snack of the Republic of Korea, and since the first pastry shop was built in 2000, Kachang Pastry Alley became widely known on Daegu area broadcasts in 2002 and began to spread by word of mouth, gaining national attention and fame as a special alleyway in 2003. Kachang Pastry Street, once renowned in Daegu and nationwide as a specialty street for confectionery, is now in difficulty due to economic recession and reduced sales. The covid-19 that began in 2019 has added to the woes of a declining pastry street. This study reflects the reality described above, diagnosing the depressed state of the Kachang pastry alley as the importance of regional distinctiveness strategies for revitalizing the regional economy increases, emphasizing regional specificities. Through prior research and theoretical investigation of Place Branding and integrated cultural spaces, three types of Place Branding are derived: place-based, content-based and environment-culture-based, and four types of integrated cultural spaces are derived: culture and art-based, commercial space-based, experience space-based and regional resource integration-based. In addition to identifying strategic links in the field of integrated design and service design, the project also proposes new directions, ideas and analyses in the area of brand space and service design, with the aim of regenerating Kachang Steamed Bun Alley through field research and design development. Through this study, as a method of enhancing the brand of this area, cultural and historical buildings that make use of the local resources are planned to be transformed into symbolic places where the uniqueness of the area can be felt through projects that connect with the residents of the area. At the same time, this study, by examining the theory of local branding, has the power to activate the economy, promote regional effects and hopefully create new value after arriving at a process that is important for future findings in the field of local brand design and marketing as well as systematic creative approaches.
Research of the Interconnection of Workflow System Based on Web Service
Gang Yuan,Rui-zhi Sun,Yong Xiang,Yin-xue Shi 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.2
In order to achieve the interconnection between different workflow management systems, it was proposed that all the distributed workflow systems would be encapsulated as web services to perform the entire business process collaboratively by the way of processes’ composition in this paper. By analyzing the comparison between the composition of processes and ordinary Web service, we studied interactive control, the parameters required to be passed through the distributed workflow systems, the workflow system service’s interfaces and its packaging. Furthermore we put forward a general method of the workflow systems interactive interfaces’ extension and the way of the workflow service’s encapsulating and invoking. By this approach, it can easily combine the processes or process fragments which deployed on different workflow systems without other agents and components. It also provides support for the interconnection of the workflow systems in distributed environment, and ultimately achieves a coordinated operation between different workflow engines.
Jianzeng Liu,Xiaohao Xu,Jingyuan Zhou,Guang Sun,Zhenzhuo Li,Lu Zhai,Jing Wang,Rui Ma,Daqing Zhao,Rui Jiang,Liwei Sun 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.6
Background: Our previous investigation indicated that the preparation of Panax ginseng Meyer(P. ginseng) inhibited melanogenesis. It comprised salicylic acid (SA), protocatechuic acid (PA), p-coumaricacid (p-CA), vanillic acid (VA), and caffeic acid (CA). In this investigation, the regulatory effects ofP. ginseng phenolic acid monomers on melanin production were assessed. Methods: In vitro and in vivo impact of phenolic acid monomers were assessed. Results: SA, PA, p-CA and VA inhibited tyrosinase (TYR) to reduce melanin production, whereas CA hadthe opposite effects. SA, PA, p-CA and VA significantly downregulated the melanocortin 1 receptor(MC1R), cycle AMP (cAMP), protein kinase A (PKA), cycle AMP-response element-binding protein (CREB),microphthalmia-associated transcription factor (MITF) pathway, reducing mRNA and protein levels ofTYR, tyrosinase-related protein 1 (TYRP1), and TYRP2. Moreover, CA treatment enhanced the cAMP, PKA,and CREB pathways to promote MITF mRNA level and phosphorylation. It also alleviated MITF proteinlevel in a-MSH-stimulated B16F10 cells, comparable to untreated B16F10, increasing the expression ofphosphorylation glycogen synthase kinase 3b (p-GSK3b), b-catenin, p-ERK/ERK, and p-p38/p38. Furthermore, the GSK3b inhibitor promoted p-GSK3b and p-MITF expression, as observed in CA-treatedcells. Moreover, p38 and ERK inhibitors inhibited CA-stimulated p-p38/p38, p-ERK/ERK, and p-MITFincrease, which had negative binding energies with MC1R, as depicted by molecular docking. Conclusion: P. ginseng roots' phenolic acid monomers can safely inhibit melanin production by bidirectionallyregulating melanin synthase transcription. Furthermore, they reduced MITF expression viaMC1R/cAMP/PKA signaling pathway and enhanced MITF post-translational modification via Wnt/mitogen-activated protein kinase signaling pathway.