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RISS 인기검색어
- 검색키워드
- 저자 : Sea Yull Chun (검색결과 5 건)
- 무료
- 기관 내 무료
- 유료
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The Relationship between Vitamin E Deficieney and the Selenium Content in Animal Organs
Chun, Sea Yull 생화학분자생물학회 1976 BMB Reports Vol.5 No.2
The relationship between selenium and vitamin E has attracted considerable attention. Detailed feeding experiments with laboratory animals have shown a certain relation betvveen the symptoms of vitamin E deficiency and selenium, but up to now the underlying mechanisms have remained obscure. We have used activation analysis to determine whether a deficiency of vitamin E alters selenium content of certain typical organs. Selenium, garlic and vitamin E supplemented diet was depleted hatched chick breeding. At an age of three weeks the chicks were killed and preparations of blood, skin, muscle, liver, kidney, testis and ovary were made. In order to assay the selenium content of some 100 preparations in a reasonable time, a fast method of analysis was chosen using the 18 sec. ^(77m) Se. Further details of the selenium vitamin E mechanism will be presented.
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Determination by Neutron Analysis of Mercury Residues in Foodstuffs
전세열,Chun, Sea-Yull Korean Society of Food Science and Technology 1971 한국식품과학회지 Vol.3 No.3
유기수은제 농약은 도열병 방제의 목적으로 다량 살포되는데 그 결과로 식품중의 수은잔류량증가로 그 피해가 예견되어 수은함량을 검색할 필요성이 요구된다. 그러나 종래 분석방법인 dithizone 비색법은 시료 분해시 수은 화합물의 손실, 유출물의 변동으로 인하여 정확도를 기대하기 어려운점이 있으나 본 연구에서 시도한 방사화 분석법에 의하면 극미량도 고감도로 정량 할 수가 있다. 본 실험에서는 곡류, 야채, 육류, 과일, 계란을 산지별로 시료를 수집하여 건조, 포장하여 vial에 넣고 열중성자속 $3.8{\times}10^{12}\;n/cm\;sec$에 15시간 조사하였다. 이 시료를 Bethge 장치로 분해시켜 수은을 증류하여 TMC 100 Channel pluse height analyzer로 $^{203}Hg$ 방사능율 0.279 MeV Photopeck로 측정하였다. 시료별로 수은 함량은 곡류 $0.033{\sim}0.250\;ppm$, 야채 $0.012{\sim}0.190\;ppm$, 닭고기 $0.04{\sim}0.07\;ppm$, 과일 $0.085{\sim}0.145\;ppm$, 계란$0.051{\sim}0.165\;ppm$, 콩나물 0.123 ppm 됨을 알게 되었다. In order to find out the degree of mercury contamination of common foodstuffs a series of determination was carried out by a highly sensitive activation analysis and the following results were obtained. 1. Polished rice contained 0.050 ppm of mercury whereas rice bran had 0.095 ppm mercury which was found in other grain in lesser degree. 2. Vegetables and fruits also contained $0.035{\sim}0.190\;ppm$ of mercury with relatively small variations from sample except persimmon which had a considerably higher amount of mercury. 3. Soybean sprout contained an unexpectantly high amount of mercury. 4. Of the animal products chicken and egg contained more mercury than the meat.
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A Study on the Incorporation of Sulfate-sulfur into Sulfur-containing Protein
이정희,전세열,신상주,Lee, Chung-Hee,Chun, Sea-Yull,Shinn, Sang-Joou 생화학분자생물학회 1971 한국생화학회지 Vol.4 No.1
고등동물에 있어서 황산염 유황의 단백질 전환은 소화관내에 기식하는 미생물의 도움으로만 가능한 것으로 알려져 있으나 본 실험에서는 고등 동물자체가 독자적으로 그와 같은 기능을 가졌는가를 구명하고져 반추초식덩물 아닌 토끼를 이용하여 표지된 황산염을 여러가지 방법으로 투여하여 그것이 단백질 합성에 이용됨을 구명하였고 토끼가 그와 같은 능력을 독자적으로 지녔음을 확인하였다. 각 기관에 있어서의 방사능 분포 상태도 아울러 조사하였다. Hinted by a symbiotic relationship between rumen microorganisms and host ruminant in utilization of inorganic sulfur compounds such as sodium sulfate and ammonium sulfate, an investigation was conducted to determine a similar symbiotic effect between ceacum microflora and host rabbit, for ammonium sulfate. Radioactivity of fecal emission was detected from the first day of administration but the radioactive compound(s) was not precipitated by barium chloride. A similar fact was found when the labelled sulfate-sulfur was administered by intraperitoneal injection. When urinary ejection was fractionated into 3 parts, namely, trichloroacetic acid precipitate, barium chloride precipitate and supernatant fractions, radioactivity was almost equally distributed into barium chloride precipitable and supernatant fractions with a minor beta-ray emission from TCA precipitated fraction. Radioactivity was detected in hairs of experimental subjects regardless the mode of drug administration. Our finding shows that angola rabbit possesses per se the capacity of such utilization independent to its ceacum microflora. Liver homogenates made 48 hours after the suspension of drug administrations showed no radioactive emission from its barium chloride precipitated fracton. Majorities of beta-ray emission was detected in the TCA precipitated fraction. A minor amount of count, detected from supernatant is interpreted as an evidence that in the liver, transformation of inorganic sulfate sulfur into other sulfur compound (s) is a pre-requisite for protein incorporation or other physiological or metabolic process. The results of in vitro test for microbial incorporation of labelled sulfate-sulfur show that when ceacum microfiora are incubated under anaerobic conditions the microorganisms can incorporate the labelled element into their cellular protein. The capacity of doing so seems to be increased by a prolonged exposure to or contact with such compound expressed otherwise, they can be trained to do so.
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표지된 황산염 유황의 유황단백질에 대한 이용에 관한 연구
이정희,전세열,신상주 ( Chung Hee Lee,Sea Yull Chun,Sang Joou Shinn ) 생화학분자생물학회 1971 BMB Reports Vol.4 No.1
Hinted by a symbiotic relationship between rumen microorganisms and host ruminant in utilization of inorganic sulfur compounds such as sodium sulfate and ammonium sulfate, an investigation was conducted to determine a similar symbiotic effect between ceacum microflora and host rabbit, for ammonium sulfate. Radioactivity of fecal emission was detected from the first day of administration but the radioactive compound (s) was not precipitated by barium chloride. A similar fact was found when the labelled sulfate-sulfur was administered by intraperitoneal injection. When urinary ejection was fractionated into 3 parts, namely, trichloroacetic acid precipitate, barium chloride precipitate and supernatant fractions, radioactivity was almost equally distributed into barium chloride precipitable and supernatant fractions with a minor beta-ray emission from TCA precipitated fraction. Radioactivity was detected in hairs of experimental subjects regardless the mode of drug administration. Our finding shows that angola rabbit possesses per se the capacity of such utilization independent to its ceacum microflora. Liver homogenates made 48 hours after the suspension of drug administrations showed no radioactive emission from its barium chloride precipitated fraction. Majorities of beta-ray emission was detected in the TCA precipitated fraction. A minor amount of count, detected from supernatant is interpreted as an evidence that in the liver, transformation of inorganic sulfate sulfur into other sulfur compound(s) is a pre-requisite for protein incorporation or other physiological or metabolic process. The results of in vitro test for microbial incorporation of labelled sulfate-sulfur show that when ceacum microflora are incubated under anaerobic conditions the microorganisms can incorporate the labelled element into their cellular protein. The capacity of doing so seems to be increased by a prolonged exposure to or contact with such compound expressed otherwise, they can be trained to do so.
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