An Alternative High-throughput Staining Method for Detection of Neutral Lipids in Green Microalgae for Biodiesel Applications

Gour Gopal Satpati,Sanjaya Kumar Mallick,Ruma Pal 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.6

A simple and high-throughput method for determining in situ intracellular neutral lipid accumulation in Chlorella ellipsoidea and Chlorococcum infusionum with flow cytometry and confocal microscopy was established by employing different solvents and a lipophilic dye, Nile red. Seven different organic solvents, acetic acid, dimethyl sulfoxide (DMSO), acetone, methanol, ethanol, n-hexane, and chloroform at different concentrations ranging from 0 to 80% (v/v) were tested. The fluorescence signal for neutral lipids was collected with a 586/42 emission filter (PE-A) and the maximum fluorescence intensity (% grandparent) was measured as 74.01 ± 4.82% for Chlorella and 70.1 ± 5.52% for Chlorococcum at 30% acetic acid (v/v). The statistical analysis of Nile red-stained cells showed a high coefficient of variation (CV), standard deviation (SD), mean, and median values in the acetic acid-based staining method, followed by DMSO, n-hexane and chloroform. Confocal microscopy revealed a high rate of accumulation of cytosolic neutral lipids when stained with Nile red and other organic solvents. Higher lipid accumulation in Fesupplemented conditions was also detected and a maximum lipid content of 57.36 ± 0.41% (4-fold) in Chlorella and 48.20 ± 0.43% (4-fold) in Chlorococcum were measured at 0.001 g/L of ferrous sulfate (FeSO4). High fluorescence intensity (75.16 ± 0.24% in Chlorella and 72.24 ± 1.07% in Chlorococcum) in Fe-treated cells confirmed the efficiency of the staining procedure.

AREA DISTORTION UNDER MEROMORPHIC MAPPINGS WITH NONZERO POLE HAVING QUASICONFORMAL EXTENSION

Bhowmik, Bappaditya,Satpati, Goutam Korean Mathematical Society 2019 대한수학회지 Vol.56 No.2

Let ${\Sigma}_k(p)$ be the class of univalent meromorphic functions defined on the unit disc ${\mathbb{D}}$ with k-quasiconformal extension to the extended complex plane ${\hat{\mathbb{C}}}$, where $0{\leq}k<1$. Let ${\Sigma}^0_k(p)$ be the class of functions $f{\in}{\Sigma}_k(p)$ having expansion of the form $f(z)=1/(z-p)+{\sum_{n=1}^{\infty}}\;b_nz^n$ on ${\mathbb{D}}$. In this article, we obtain sharp area distortion and weighted area distortion inequalities for functions in ${\sum_{k}^{0}}(p)$. As a consequence of the obtained results, we present a sharp upper bound for the Hilbert transform of characteristic function of a Lebesgue measurable subset of ${\mathbb{D}}$.

Effect of oxygen containing compounds in uranium tetrafluoride on its non-adiabatic calciothermic reduction characteristics

Gupta, Sonal,Kumar, Raj,Satpati, Santosh K.,Sahu, Manharan L. Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.6

Uranium ingot is produced by metallothermic reduction of uranium tetrafluoride using magnesium or calcium as reductant. Presence of oxygen containing compounds viz. uranyl fluoride and uranium oxide in the starting uranium fluoride has a significant effect on the firing time, final temperature of the charge, slag-metal separation and hence the metal recovery. As reported in the literature, the maximum tolerable limit for uranyl fluoride in the UF₄ is 2.5 wt% and limit for uranium oxide content is in the range 2-3 wt%. No theoretical or experimental basis is available till date for these limits. Analyses have been carried out in this study to understand the effect of UO₂F₂ concentration in the starting fluoride on the final temperature of the products and thus the reduction characteristics. UF₄ having uranyl fluoride concentration, less than as well as more than 2.5 wt%, have been investigated. Thermodynamic calculations have been carried out to arrive at a general expression for the final temperature attained by the products during calciothermic reduction of UF₄. Finally, an upper limit for the oxygen containing impurities has been estimated using the CaO-CaF₂ phase diagram.

Area distortion under meromorphic mappings with nonzero pole having quasiconformal extension

Bappaditya Bhowmik,Goutam Satpati 대한수학회 2019 대한수학회지 Vol.56 No.2

Let $\Sigma_k(p)$ be the class of univalent meromorphic functions defined on the unit disc $\mathbb D$ with $k$-quasiconformal extension to the extended complex plane $\widehat{\mathbb C}$, where $0\leq k < 1$. Let $\Sigma_k^0(p)$ be the class of functions $f \in \Sigma_k(p)$ having expansion of the form $f(z)= 1/(z-p) + \sum_{n=1}^{\infty}b_n z^{n}$ on $\mathbb D.$ In this article, we obtain sharp area distortion and weighted area distortion inequalities for functions in $\Sigma_k^0(p)$. As a consequence of the obtained results, we present a sharp upper bound for the Hilbert transform of characteristic function of a Lebesgue measurable subset of $\mathbb D$.

Study of the Changes in Composition of Ammonium Diuranate with Progress of Precipitation, and Study of the Properties of Ammonium Diuranate and its Subsequent Products Produced from both Uranyl Nitrate and Uranyl Fluoride Solutions

Subhankar Manna,Raj Kumar,Santosh K. Satpati,Saswati B. Roy,Jyeshtharaj B. Joshi 한국원자력학회 2017 Nuclear Engineering and Technology Vol.49 No.3

Uranium metal used for fabrication of fuel for research reactors in India is generally produced by magnesio-thermic reduction of UF4. Performance of magnesio-thermic reaction and recovery and quality of uranium largely depends on properties of UF4. As ammonium diuranate (ADU) is first product in powder form in the process flow-sheet, properties of UF4 depend on properties of ADU. ADU is generally produced from uranyl nitrate solution (UNS) for natural uranium metal production and from uranyl fluoride solution (UFS) for low enriched uranium metal production. In present paper, ADU has been produced via both the routes. Variation of uranium recovery and crystal structure and composition of ADU with progress in precipitation reaction has been studied with special attention on first appearance of the precipitate Further, ADU produced by two routes have been calcined to UO3, then reduced to UO2 and hydroflorinated to UF4. Effect of two different process routes of ADU precipitation on the characteristics of ADU, UO3, UO2 and UF4 were studied here.

Influence of ion implantation on depth dependent phase transition in TiO2 films, anatase nanostructures and photo-absorption behavior

Manna Ashis K.,Joshi Shalik R.,Satpati B.,Dash P.,Chattaraj Ananya,Srivastava S.K.,Kanjilal A.,Kanjilal D.,Varma Shikha 한국물리학회 2022 Current Applied Physics Vol.43 No.-

The present study explores the role of Ti ion implantation in structural phase transition in TiO2 thin films. Raman and TEM results reveal that after implantation Rutile phase in films increases at the expense of Anatase nanostructures. Though the as-deposited films display the presence of bigger anatase nanoparticles, after implantation, predominantly phonon confined smaller (~ 8 nm in size) anatase nanostructures are observed. GIXRD and Raman results further reflect presence of a critical fluence, 1 × 1013 ions/cm2, where the initial transformation from anatase to Rutile phase is observed. The role of Oxygen vacancies, in this transformation, has been explored here by XPS. Modifications in UV–Vis and Bandgap results show rich behavior which also reflects phase transformation at the critical fluence. Results further indicate that the phase transition gets first initiated deeper in the film and later on the surface. Interestingly, aggregation of larger Anatase nano-particles appears to be responsible for the structural transformation as observed here.

Influence of abiotic factors on seasonal incidence of pests of tasar Silkworm Antheraea mylitta D.

( Aruna A. Siddaiah ),( Rajendra Prasad ),( Suresh Rai ),( Omprakash Dubey ),( Subrat Satpaty ) 한국잠사학회 2014 International Journal of Industrial Entomology Vol.29 No.1

Rearing of tropical tasar silkworm, Antheraea mylitta Drury is mainly conducted in outdoor on Terminalia tomentosa W. & A. a nature grown primary host plant available in forest and also on raised primary host plant Terminalia arjuna Bedd. Temperature, relative humidity and rainfall are the main environmental factors for occurrence of pests (parasites and predators) of tasar silkworm during I, II and III crop rearing in the tropical tasar producing zones. The present study was aimed to study the influence of abiotic factors on prevalence of tasar silkworm pests. The study was conducted at different agro-climatic regions viz., Central Tasar Research &Training Institute, Ranchi, Jharkhand, Regional Extension Centre, Katghora, Chattisgarh and Regional Extension Centre, Hatgamaria during 2010-13 covering 3 seed crop and 6 commercial crops. Data on incidence of tropical tasar silkworm endo-parasitoids like Uzi Fly, Blepharipa zebina Walker and Ichneumon fly (Yellow Fly), Xathopimpla pedator, Fabricius and Predators such as Stink bug ( Eocanthecona furcellata Wolf), Reduviid bug ( Sycanus collaris Fabricius) and Wasp ( Vespa orientalis Linnaeus) was recorded Weekly. The meteorological data was collected daily. Data was collected from 4 different agro-climatic zones of tasar growing areas. Analysis of the data revealed a significant negative correlation between abiotic factors and incidence of ichneumon fly and uzi fly. Based on the 3 years data on prevalence of pests region-wise pest calendars and prediction models were developed.

Influence of abiotic factors on seasonal incidence of pests of tasar Silkworm Antheraea mylitta D.

Siddaiah, Aruna A.,Prasad, Rajendra,Rai, Suresh,Dubey, Omprakash,Satpaty, Subrat,Sinha, Ravibhushan,Prsad, Suraj,Sahay, Alok Korean Society of Sericultural Science 2014 International Journal of Industrial Entomology Vol.29 No.1

Rearing of tropical tasar silkworm, Antheraea mylitta Drury is mainly conducted in outdoor on Terminalia tomentosa W. & A. a nature grown primary host plant available in forest and also on raised primary host plant Terminalia arjuna Bedd. Temperature, relative humidity and rainfall are the main environmental factors for occurrence of pests (parasites and predators) of tasar silkworm during I, II and III crop rearing in the tropical tasar producing zones. The present study was aimed to study the influence of abiotic factors on prevalence of tasar silkworm pests. The study was conducted at different agro-climatic regions viz., Central Tasar Research &Training Institute, Ranchi, Jharkhand, Regional Extension Centre, Katghora, Chattisgarh and Regional Extension Centre, Hatgamaria during 2010-13 covering 3 seed crop and 6 commercial crops. Data on incidence of tropical tasar silkworm endo-parasitoids like Uzi Fly, Blepharipa zebina Walker and Ichneumon fly (Yellow Fly), Xathopimpla pedator, Fabricius and Predators such as Stink bug (Eocanthecona furcellata Wolf), Reduviid bug (Sycanus collaris Fabricius) and Wasp (Vespa orientalis Linnaeus) was recorded Weekly. The meteorological data was collected daily. Data was collected from 4 different agro-climatic zones of tasar growing areas. Analysis of the data revealed a significant negative correlation between abiotic factors and incidence of ichneumon fly and uzi fly. Based on the 3 years data on prevalence of pests region-wise pest calendars and prediction models were developed.

Effect of process parameters on the recovery of thorium tetrafl uoride prepared by hydrofl uorination of thorium oxide, and their optimization

Raj Kumar,Sonal Gupta,Sourabh Wajhal,S.K. Satpati,M.L. Sahu 한국원자력학회 2022 Nuclear Engineering and Technology Vol.54 No.5

Liquid fueled molten salt reactors (MSRs) have seen renewed interest because of their inherent safetyfeatures, higher thermal efficiency and potential for efficient thorium utilisation for power generation. Thorium fluoride is one of the salts used in liquid fueled MSRs employing TheU cycle. In the presentstudy, ThF4 was prepared by hydro-fluorination of ThO2 using anhydrous HF gas. Process parameters viz. bed depth, hydrofluorination time and hydrofluorination temperature, were optimized for the preparation of ThF4 in a static bed reactor setup. The products were characterized with X-Ray diffraction andexperimental conditions for complete conversion to ThF4 were established which also corroborated withthe yield values. Hydrofluorination of ThO2 at 450 C for half an hour at a bed depth of 6 mm gave thebest result, with a yield of about 99.36% ThF4. No unconverted oxide or any other impurity was observed. Rietveld refinement was performed on the XRD data of this ThF4, and Chi2 value of 3.54 indicated goodagreement between observed and calculated profiles.

Large Spin Diffusion Length in an Amorphous Organic Semiconductor

Shim, J. H.,Raman, K. V.,Park, Y. J.,Santos, T. S.,Miao, G. X.,Satpati, B.,Moodera, J. S. American Physical Society 2008 Physical Review Letters Vol.100 No.22

<P>We directly measured a spin diffusion length (lambdas) of 13.3 nm in amorphous organic semiconductor (OS) rubrene (C42H28) by spin polarized tunneling. In comparison, no spin-conserved transport has been reported in amorphous Si or Ge. Absence of dangling bond defects can explain the spin transport behavior in amorphous OS. Furthermore, when rubrene barriers were grown on a seed layer, the elastic tunneling characteristics were greatly enhanced. Based on our findings, lambdas in single-crystalline rubrene can be expected to reach even millimeters, showing the potential for organic spintronics development.</P>