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Subunit Interactions of Vertebrate Lactate Dehydrogenase: I. Immunochemistry of Subunits
Park, Sang-Yoon,Yum, Jung-Joo,Kim, Sang-Yeop The Korean Society for Integrative Biology 1979 동물학회지 Vol.22 No.3
경골어류 드렁허리와 가물치에서 젖산수소이탈효소의 세가지 동질사량체를 순수 정제하였다. 이들에 대한 항혈청을 얻어 젖산수소이탈효소와 면역화학적 반응을 실시하였다. 하부단위체의 활성자리는 항원결정군에 속해 있지 않으며, 항혈청내의 항체 또는 미지의 물질이 하부단위체의 하전을 변화시키며, 두 하부단위체는 공통의 항원결정군을 갖고 있음이 확인되었다. Two homotetrameric lactate dehydrogenase isozymes from Fluta alba and one from Ophicephalus argus were purified by combination of gel filtration and DEAE-cellulose chromatogrphy. The final preparations were isozymically pure and used to elicit antibodies in rabbits. The immunochemical reactivities demonstrated that the amino acids of active site is not to be included in the antigenic determinants, that antibodies or unknown component of immunized rabbit serum might be responsible for the electrophoretic abnormality and that two subunits share common antigenic determinants, reflecting that these polypeptides have a common evolutionary origin.
Comparative Immunochemistry of a Fraction in Soluble Muscle Proteins
Park, Sang Yoon,Yum, Jung Joo,Kim, Sang Yeop 成均館大學校 1979 論文集 Vol.26 No.-
파충류 젖산수소이탈효소와 물리화학적 성질이 유사한 수용성 근단백질을 유혈목이(Rhabdophis tigrinus)로부터 정제하였으며 토끼로부터 이에 대한 항혈청을 얻었다. 척추동물 19종의 골격근 추출액과 항혈청의 반응정도를 면역확산 방법에 의하여 얻었다. 포유류와 조류에서는 흡수반응이 없었으나 파충류에서는 모두 반응을 나타냈다. 쇠살모사(Agkistrodon caliginosus)를 신종으로 기재한 것은 분자수준에서도 타당한 것 같으며 좀개구리(Rana dybowskii)는 Rana 속의 종들중 가장 유연관계가 먼 것 같다. A protein fraction sharing similar physicochemical properties with M_2H_2 lactate dehydrogenase isozyme was purified from the Rhabdophis tigrinus and injected into rabbits. The resulting antiserum was tested for reactivity with crude extract of skeletal muscle in nineteen vertebrate species. No cross-reactivity was made in the immunodiffusion tests with species of classes Mammalia and Aves examined. All reptiles revealed the immunochemical reactivities and Agkistrodon caliginosus was reevaluated as a new species at the molecular level. Rana dybowskii is considered to be a species less related to other ranids.
( Sang-yeop Lee ),( Won-seok Yang ),( Se-won Park ),( Gun-ho Han ),( Yong-chil Seo ),( Woo-hyen Kim ),( Young-sik Yoon ),( Won-sik Lee ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 심포지움 Vol.2019 No.1
Solid refuse fuel (SRF) manufacturing facilities, residues are buried at a rate of 30~45% of incoming amount or some are disposed of by incineration facilities. It is known that landfill can increase the pollution load and it takes about 20% of total facility operation cost due to landfill disposal cost. In the study residue waste from SRF manufacturing plant was attempted to combust, the bottom ash generated from incineration tests was utilized aggregates to fabricate bricks to assess the applicability. The variation in bottom ash properties influences the characteristics of bricks and also it could be hazardous for environment. So, before making bricks from generated bottom ash, basic characteristic of bottom ash such as ignition loss and heavy metal leaching test must be analyzed. These bricks were manufactured by substitution of stone powder and sand. These can be used as an interlocking block for the sidewalk and open space. Finally we analyzed the basic characteristics of the bottom ash and analyzed the water absorption, bending strength, compressive strength, and dimensions of the mixed bricks. Following these results, as comparing with standard interlocking block, recycling brick satisfied with the less than 10% abosrption rate and more than 5Mpa flexural strenghth. we could assess the applicability of these bricks manufactured.
Immunological Comparison of Muscle Soluble Proteins in Lower Vertebrates
Park, Sang-Yoon,Kim, Sang-Yeop,Yum, Jung-Joo 成均館大學校 1977 論文集 Vol.24 No.-
담수어류 가물치 수용성 근단백질에 대한 항혈청을 얻어 척추동물 11종의 근단백질과 항원-항체반응을 실시하였다. 잉어, 붕어, 메기 및 드렁허리는 가물치와 가까운 유연관계에 있고 뱀장어, 미꾸라지 및 바다뱀장어는 가물치와 유연관계가 적은 종들로 밝혀졌다. 하등척추동물 단백질의 3차구조를 유지하고 있는 단백질이 조류에서는 존재하지 않는 것 같다. 가물치의 수용성 근단백질에는 다발성 항원이 존재함이 확인되었다. Antisera against whole muscle soluble proteins in fresh-water Ophicephalus argus were prepared and tested for antigen-antibody reactivity with crude muscle extracts of eleven vertebrate species. Teleost of Cyprinus carpio, Carassius carassius, Parasilurus asotus and Fulata alba seem to be closely related to O. argus. Teleost of Anguilla japonica, Misgurnus mizolepis and Ophisurus macrorhynchus are considered to be species less related to O. argus. It is likely that there is no protein in avian species which has maintained the three dimensional structure of lower vertebrate protein. There are also multispecific antigens in O. argus proteins.
Immunological Comparison of Reptilian Plasma Albumins and Hemoglobins
Park, Sang-Yoon,Kim, Sang-Yeop,Yum, Jung-Joo The Korean Society for Integrative Biology 1977 동물학회지 Vol.20 No.4
유혈목이(Rhabdophis tigrimus)의 혈장알부민을 polythylene glycol과 ethanol 침전 방법에 의해 정제하였고 혈색소는 agar-gel 전기영동방법에 의해 정제하였다. 이 두 단백질과 유혈목이 혈장을 각각 토끼에 주사하여 항혈청들을 얻었으며 포유류 1종, 조류 1종, 파충류 9종, 및 양서류 1종의 혈장 및 혈구파쇄물과 면역확산 및 면역 전기영동을 실시하였다. 혈장알부민과 혈장에 대한 항혈청들은 유혈목이 혈장알부민과만 반응을 나타냈고 다른종의 혈장알부민과는 반응을 나타내지 않았다. 혈색소에 대한 항혈청은 8종의 사류 혈색소와 강한 반응을 나타냈다. 파충류의 혈장알부민의 구조는 상당히 빠르게 변형되어져 왔고 파충류의 경우 혈색소 분자는 강한 구조상의 상동성을 갖고 있는 것 같다. 그러므로 파충류의 경우 혈색소 분자의 구조는 혈장알부민의 구조보다 느리게 변형되어져 온 것으로 생각된다. Plasma albumin and hemoglobin were purified from the blood of Rhabdophis tigrinus. Both purified proteins and R. tigrinus crude plasma were injected into rabbits. The resulting antisera were tested for reactivity with plasma albumin and hemoglobin from, eight species of squamate and four species of non-squamate-vertebrate. Reactivity was detected qualitatively by immunodiffusion tests and immunoelectrophoreses. Antisera against plasma albumin and crude plasma reacted only with R. tigrinus plasma albumin. No antigen-antibody reactions were detected in plasma albumins of other species. Antiserum against R. tigrinus hemoglobin reacted strongly with eight squamate hemoglobins. It is likely that the reptilian plasma albumin has been experiencing rapid structural change and the reptilian hemoglobin molecules seem to be of high homogeneity. Thus, by immunological evidences, the structure of the hemoglobin molecule is considered to have been changing at slower rate than that of the plasma albumin in reptiles.
Demonstration Test of Full System Decontamination and Its Operational Review
Sung Hyun Lee,Jung-Hyun Lee,Ju-Hyeon Park,Sung-Sik Yoon,Jeong-Yeop Cha,Seung-Ki Lee,Sung-Su Kim,Chung-kyu Lee,Ki-Chul Kim 한국방사성폐기물학회 2022 한국방사성폐기물학회 학술논문요약집 Vol.20 No.2
In this research, KPS manufactured Full System Decontamination (FSD) equipment, which is consisted of Oxidizing Agent Manufacturing System (OAMS), Chemical Injection System (CIS), RadWaste Treatment System (RWTS), Chemical Waste Decomposition & Treatment System (CWDS) and conducted demonstration test to prepare Decontamination and Decommissioning (D&D) project of Kori nuclear power plant in Korea. Each equipment of FSD was modularized due to the limited size of equipment hatch of Kori nuclear power plant. To simulate the expected circumstances in nuclear power plant such as usage of heater or position of each equipment, additional equipment was used. The chemical concentration and flow rate of process water for FSD were used as mentioned in the previous study by KHNP CRI. FSD was conducted for three cycles and each cycle was consisted of oxidation, reduction, chemical decomposition and purification. Oxidation and reduction process were conducted at 90°C. Chemical decomposition and purification process were conducted at 40°C due to the damage of UV lamp and IX by the heat. Total volume of process water for FSD demonstration test was 2.5 m2. KPS conducted decontamination performance review by calculating thickness reduction and weight loss of installed specimen. Operational review was conducted as if FSD test was conducted in the field based on the result of demonstration test. One of the most prioritized features is the workers’ safety. Also, the appropriate position of equipment needs to be considered to meet the required specification of component.