개에서 발생한 기관 허탈의 진단과 관리

이새미,윤기영,서경원,최호정,정성목,송근호,조종기,박성준 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2012 動物醫科學硏究誌 Vol.19 No.1

A 11-year-old Yorkshire terrier anesthetized for computed tomography. The dog showed cyanosis and syncope during postanesthetic recovery, therefore drug administrated parenterally. Goose-honking was noticed after full recovery. As a result of survey radiography and fluoroscopy, grade 4 tracheal collapse was diagnosed. Prescription for the dog was oral administration of hydrocodone 0.22 mg/kg. theophylline 20 mg/kg, prednisone 0.5 mg/kg, amoxicillin 22 mg/kg and chlorpheniramine 2 mg/kg twice a day for 3 days. A 10-year-old Pomeranian was diagnosed as grade 2 traceal collapse at the local animal hospital 2 years before being referred to the veterinary teaching hospital of Chungnam National University. On physical examination, goose-honking, coughing and intermittent open-mouth breathing. Tracheal narrowing was observed on survey einradiography and fluoroscopy. Oral administration of theophylline 13 mg/kg, enrofloxacin 5 mg/kg, chlropheniramine 0.5 mg/kg twice a day was prescribed for 2 weeks.

대장수술 후에 발생한 급성신부전의 위험인자

이혜미,황창재,김재황,김흥대,박대팔,서일숙,송선옥,김세연,이덕희,지대림 영남대학교 의과대학 2007 Yeungnam University Journal of Medicine Vol.24 No.2

연구 배경 : 급성 신부전은 술 후 발생할 수 있는 합병증 중에서 사망률은 큰 영향을 줄 수 있으므로 이 연구를 통해 위험인자를 알아보려고 한다. 재료 및 방법 : 2004년 1월부터 2006년 12월까지 3년간 영남대학교 의과대학 부속병원에서 대장 수술을 받은 570명의 환자들을 대상으로 하였다. 환자들의 성별과 연령, 미국 마취과학회 신체등급, 동반질환, 수술의 종류, 응급수술의 여부, 수술 시간, 재수술의 여부, 술 중의 투약 상황, 술 후에 자가 통증 조절기에 사용한 약제, 술 중에 저혈압의 유무, 수혈 여부, 술 후 기계적 환기를 시행한 경우를 비교하였다. 결과 : 비교 결과에서 성별과 수술의 종류, 응급 수술의 여부, 개복여부 등에는 유의한 차이를 보이지 않았다. 반면 환자의 나이와 수술 시간, 재수술의 여부, 술중에 이뇨제 등을 사용한 경우, 술 중에 저혈압의 유무, 술 후에 기계적 환기를 시행한 경우 등에는 유의한 차이를 보였다. 결론 : 이상의 결과로 급성신부전의 원인은 어느 한 가지라고 하기 보다는 위험인자에 얼마나 노출되어 있는가가 결정하는 것 같고, 그 원인들이 모여서 상승효과를 내는 것 같다. 그러므로 수술 전부터 많은 위험에 노출된 환자는 술 중 더 적극적인 감시를 시행하여 수술 후의 합병증 발생을 줄이는 노력을 기울여야 하겠다. Background : Acute renal failure is one of the leading causes of postoperative morbidity and mortality. The purpose of this study was to determine the risk factors that are associated with acute renal failure after colorectal surgery. Materials and Methods : Five hundred seventy patients who operated colorectal surgery at the Yeungnam University Medical Center over three years from 2004 to 2006 were enrolled in this study. The effects of gender, age, ASA classification, concomitant disease, surgery type and duration, reoperation, urogenital manipulation, medication, hypotension, hypovolemia, transfusion, and postoperative ventilatory care on the occurrence of acute renal failure after colorectal surgery were studied. Results : The major risk factors of acute renal failure after colorectal surgery were age of patients (P=0.003), ASA classification (P<0.001), concomitant disease (P<0.001), duration of the time surgery (P=0.034), reoperation (P=0.001), use of intraoperative diuretics (P=0.005), use of postoperative diuretics (P<0.001), intraoperative hypotension (P=0.018), intraoperative transfusion (P<0.001), postoperative transfusion (P<0.001), and postoperative ventilatory care (P=0.001). Conclusion : Multiple factors cause synergistic effects on the development of acute renal failure after colorectal surgery. Therefore, efforts to reduce the risk factors associated with acute renal failure are needed. In addition, intensive postoperative care should be provided to all patients.

Alprazolam함유 poly(D,L-lactic acid) microsphere의 제조 및 평가

용철순,오두만,권미라,박새해 영남대학교 약품개발연구소 1996 영남대학교 약품개발연구소 연구업적집 Vol.6 No.-

Poly(D.L-lactic acid)(PLA) microspheres containing alprazolam(APZ) were prepared by a solvent-emulsion evaporation method and their release patterns were invostigated in vitro. Various batches of microspherss with different size and drug content were obtained by changing the ration of APZ to PLA, PLA concentration in the dispersed phase and stirring rate. Rod-like APZ crystals on microsphere surface, which were released rapidly and could act as a loading dose, were observed with increasing drug content. The release rate was increased with increase in drug contents and decresase in the molecular weight of PLA. The release rate of APZ for long-acting injectable delivery system in vitro, which would aid in predicting in vivo release profile, could be controlled by properly optimizing various factors affecting characteristics of microspheres.

A case of multiple juvenile xanthogranuloma with involvement of the glans penis

( Sae Mi Park ),( Hyun Ji Kim ),( Young Bok Lee ),( Jin Wou Kim ),( Dong Soo Yu ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Juvenial xanthogranuloma (JXG) is a rare benign skin lesion arising in early childhood, showing usually self-limiting course. It is the commonest form of non-Langerhans cell histiocytosis and typically presents as a well-defined, solitary or multiple, dome-shaped, yellow-red papulonodules. Clinically it generally arises on the head and neck region followed by the trunk and upper extremities. It can also affect other organs involving eye, lung, or liver and be associated with systemic disease such as neurofibromatosis or juvenile leukemia. A 10-month old male presented with multiple dome-shaped orange-colored papules on the lower abdomen, scrotum and glans penis and this lesion had been developing since birth. Histopathologic examination revealed the dermal infiltration of histiocytes with foam cells and multinucleated giant cells. Also, tumor cells showed positivity for CD 68, which was compatible with the diagnosis of xanthogranuloma. We performed laboratory and imaging studies including ophthalmology examination and the patient showed no systemic involvement. Although JXG can develop anywhere on the skin, based on the previous reports, penile involvement is very rare in infants. Herein, we report on a case of juvenile xanthogranuoma involving the glans penis in a 10-month old infant.

Eccrine porocarcinoma of the scalp: A rare case report

( Sae Mi Park ),( Soo Young Lee ),( Young Bok Lee ),( Jin Wou Kim ),( Dong Soo Yu ) 대한피부과학회 2016 대한피부과학회 학술발표대회집 Vol.68 No.1

Eccrine porocarcinoma is a rare malignant neoplasm of the skin that originates from the intraepidermal portion of the eccrine sweat glands with various clinicopathologic presentations. It is rarely reported in literature and represents 0.005-0.01% of all skin tumors. Clinically it presents with a verrucous or polypoid nodule mimicking other skin tumor such as wart and squamous cell carcinoma. It commonly affects late adulthood between 60 to 80 years of age, and arises frequently on the upper extremities and rarely on head and neck region. A 55-year-old woman presented with asymptomatic, solitary, 5x4x3cm-sized, irregular-shaped, erythematous hyperkeratotic protruding mass on the scalp for 20 years. The patient reported that the tumor had increased rapidly in past several months and accompanied by oozing and bleeding. The histopathologic finding revealed atypical basaloid cells with ductal structures in tumor nests infiltrating dermis, showing frequent mitosis and eccrine differentiation. Immunohistochemistry was positive for CK5/6, CK19, Epithelial Membrane Antigen. The patient was diagnosed with eccrine porocarcinoma and PET-CT was done for staging. The imaging study showed no metastasis and the patient underwent wide surgical excision. To the best of our knowledge, there have been few cases of porocarcinoma arising on scalp. Herein, we report a rare case of porocarcinoma arising on the scalp in a 58-year-old female patient.

Amelioration of inflammatory responses by <i>Socheongryong-Tang</i> , a traditional herbal medicine, in RAW 264.7 cells and rats

Park, Sang Mi,Lee, Tae Hoon,Zhao, Rongjie,Kim, Youn Sook,Jung, Ji Yun,Park, Chung A.,Jegal, Kyung Hwan,Ku, Sae Kwang,Kim, Jae Kwang,Lee, Chul Won,Kim, Young Woo,Cho, Il Je,An, Won G.,Kim, Sang Chan D.A. Spandidos 2018 International journal of molecular medicine Vol.41 No.5

<P>Socheongryong-Tang (SCRT) is a natural medicine prescription that has been mainly used in East Asia for the treatment of inflammatory disorders, including asthma and allergic rhinitis. The present study evaluated the anti-inflammatory effects of SCRT on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and in a rat model of carrageenan (CA)-induced paw edema. Levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in the culture supernatant were quantified and nitric oxide (NO) production was monitored. In addition, the effect of SCRT on the protein expression of nuclear factor-κB (NF-κB), mitogen-activated protein kinases (MAPKs), inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) was assessed by western blot analysis. Furthermore, the effects of SCRT on acute inflammation <I>in vivo</I> and changes in the histomorphometry and histopathology of paw skin were observed using CA-treated rats. SCRT (1 mg/ml) inhibited the LPS-induced changes in the protein expression of NF-κB, JNK, ERK1/2, iNOS and COX-2, as well as the production of NO, PGE<SUB>2</SUB> and cytokines. In the rat paw edema assay, administration of 1 g/kg of lyophilized powder obtained from the aqueous extracts of SCRT for 3 consecutive days inhibited the CA-induced increases in skin thickness, mast cell degranulation, and infiltration of inflammatory cells in the ventral and dorsal pedis skin within 4 h. These results demonstrated that SCRT exerts its anti-inflammatory activities in LPS-stimulated RAW 264.7 cells through decreasing the production of inflammatory mediators, including PGE<SUB>2</SUB>, NO and cytokines, via suppression of the NF-κB and JNK and ERK1/2 signaling pathways. In addition, the data of the CA-induced paw edema indicated an anti-edema effect of SCRT. SCRT (1 g/kg) reduced acute edematous inflammation through inhibition of mast cell degranulation and infiltration of inflammatory cells. Therefore, the present study provided scientific evidence for the anti-inflammatory activities of SCRT as well as the underlying mechanisms.</P>

NecroX-5 prevents breast cancer metastasis by AKT inhibition via reducing intracellular calcium levels

Park, Jin-Hee,Kim, Hyoung Kyu,Jung, Hana,Kim, Ki Hyang,Kang, Mi Seon,Hong, Jun Hyuk,Yu, Byeng Chul,Park, Sungjae,Seo, Su-Kil,Choi, Il Whan,Kim, Soon Ha,Kim, Nari,Han, Jin,Park, Sae Gwang Spandidos Publications 2017 International journal of oncology Vol.50 No.1

<P>A major goal of breast cancer research is to prevent the molecular events that lead to tumour metastasis. It is well-established that both cytoplasmic and mitochondrial reactive oxygen species (ROS) play important roles in cell migration and metastasis. Accordingly, this study examined the molecular mechanisms of the anti-metastatic effects of NecroX-5, a mitochondrial ROS scavenger. NecroX-5 inhibited lung cancer metastasis by ameliorating migration in a mouse model. In human cancer cells, the inhibition of migration by NecroX-5 is cell type-dependent. We observed that the effect of NecroX-5 correlated with a reduction in mitochondrial ROS, but mitochondrial ROS reduction by MitoQ did not inhibit cell migration. NecroX-5 decreased intracellular calcium concentration by blocking Ca2+ influx, which mediated the inhibition of cell migration, AKT down regulation and the reduction of mitochondrial ROS levels. However, the reduction of mitochondrial ROS was not associated with supressed migration and AKT downregulation. Our study demonstrates the potential of NecroX-5 as an inhibitor of breast cancer metastasis.</P>

<i>Sipjeondaebo-tang</i> Alleviates Oxidative Stress-Mediated Liver Injury through Activation of the CaMKK2-AMPK Signaling Pathway

Park, Sang Mi,Kim, Sung Woo,Jung, Eun Hye,Ko, Hae Li,Im, Chae Kwang,Lee, Jong Rok,Byun, Sung Hui,Ku, Sae Kwang,Kim, Sang Chan,Park, Chung A,Kim, Kwang Joong,Cho, Il Je Hindawi 2018 Evidence-based Complementary and Alternative Medic Vol.2018 No.-

<P><I>Sipjeondaebo-tang</I> (SDT) is used frequently as a herbal prescription to treat deficiency syndromes in traditional Korean medicine. We investigated the hepatoprotective effects of SDT against oxidative stress and attempted to clarify the underlying molecular mechanisms. SDT pretreatment reduced arachidonic acid (AA) plus iron-mediated cytotoxicity in a concentration-dependent manner and prevented changes in apoptosis-related protein expression. In addition, SDT pretreatment significantly reduced glutathione depletion, hydrogen peroxide production, and mitochondrial dysfunction via treatment with AA plus iron. SDT increased the phosphorylation of AMP-activated protein kinase (AMPK) in accordance with the phosphorylation of Ca<SUP>2+</SUP>/calmodulin-dependent protein kinase kinase 2 (CaMKK2). Experiments using an AMPK chemical inhibitor (Compound C) or CaMKK2 chemical inhibitor (STO-609) suggested that the CaMKK2-AMPK signaling pathway contributes to SDT-mediated protection of mitochondria and cells. Moreover, administration of SDT for 4 consecutive days to mice significantly reduced the alanine aminotransferase and aspartate aminotransferase activities induced by carbon tetrachloride, and the numbers of degenerated hepatocytes, infiltrated inflammatory cells, nitrotyrosine-positive cells, and 4-hydroxynonenal-positive cells in liver tissue. Therefore, SDT protects hepatocytes from oxidative stress via CaMKK2-dependent AMPK activation and has the therapeutic potential to prevent or treat oxidative stress-related liver injury.</P>

Safety & pain reducing effectiveness of facial subcutaneous injection of Cross-linked dextran and polymethylmethacrylate (PMMA) mixture with lidocaine to the nasolabial folds; single center, double blinded, one-arm, 3weeks follow up study

( Sae Mi Park ),( Dae Hong Kim ),( Eun Jong Song ),( Kum Hee Jung ),( Jun Young Lee ),( Young Bok Lee ),( Jin Wou Kim ),( Dong Soo Yu ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: In a filler injections, one of patients` main concerns injections pertains to pain and discomfort, especially for the first time patient. Objectives: To evaluate pain at the injection site during and after the injection of Cross-linked dextran and polymethylmethacrylate (PMMA) with lidocaine mixture or the same filler without lidocaine and to evaluate outcomes after 3 weeks. Methods: In a double-blinded, prospective, randomized, split-face design trial, Cross-linked dextran and PMMA mixture with lidocaine was injected in one nasolabial fold(NLF), and the same filler without lidocaine was injected in the contralateral NLF of 14 enrolled patients. Injection site pain was measured using a visual analogue scale at injection (time 0) and 15, 30, 45, and 60 minutes after injection. Patients were asked to return for an evaluation after 3 weeks and to complete a self-assessment questionnaire during the follow-up visit. Results: There was more than 10mm(VAS score) less pain associated with the dermal gel with lidocaine than with the same filler without lidocaine at all time points (p<.001). The greatest difference in pain was recorded at the time of injection, and then the effect gradually declined over the 60-minute period. Both fillers were well tolerated, and there was no difference in outcome after 3 weeks. Conclusion: Studies have shown that lidocaine enhances treatment comfort and optimizes the injection experience while maintaining a similar safety and effectiveness profile.

Immunomodulatory effects of Ul-Jin thermomineral water on Oxazolone-induced atopic dermatitis murine model

( Sae Mi Park ),( Dae Hong Kim ),( Eun Jong Song ),( Kum Hee Jung ),( Jun Young Lee ),( Young Bok Lee ),( Dong Soo Yu ),( Jin Wou Kim ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2

Background: Althoug the therapeutic effect of Balneotherapy for atopic dermatitis is controversial, many atopic patients are visiting thermomineral springs and present clinical improvements. Objectives: The aim of this study is to evaluate the therapeutic effect and the immunologic mechanism of balneotherapy in atopic dermatitis. Methods: Oxazolone-induced atopic dermatitis murine model was used for evaluating the therapeutic effect of balneotherapy with Ul-Jin thermomineral water. Confocal microscopic imaging analysis was performed for lesional expression of IL-4, IL-17, CD4, Foxp3, and TNF-a. Lesional mRNA expression of IL-33, TSLP, and Foxp3 was evaluated by real-time reverse transcriptase polymerase chain reaction method. Histologic evaluation was also performed. Results: Comparing with distilled water balneotherapy group, the lesional expression of IL-4, TNF-a, IL-17 was decreased in mineral water balneotherapy group by confocal microscopic examination. However, Confocal microscopic evaluation of CD4 and Foxp3 showed increase in mineral water balneotherapy. The lesional mRNA levels of IL-33 and TSLP showed reduction in mineral water balneotherapy group, whereas the level of mRNA of Foxp3 increased in mineral water balneotherapy group. Conclusion: These results suggest that balneotherapy can be an effective and safe treatment option in atopic dermatitis.