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권상우(S. W. Kwon),정원택(W. T. Jeong),공유식(Y. S. Kong),김영식(Y. S. Kim),김선진(S. J. Kim) 한국동력기계공학회 2004 한국동력기계공학회 학술대회 논문집 Vol.- No.-
Dissimilar friction welding were produced using 15㎜ diameter solid bar in chrome molybedenum steel(KS SCM440) to carbon steel(KS S45C) to investigate their mechanical properties. The main friction welding parameters were selected to endure good quality welds on the basis of visual examination, tensile tests, Virkers hardness surveys of the bond of area and HAZ and macrostructure investigations. The specimens were tested as-welded and post-weld heat-treated. The tensile strength of the friction welded steel bars was increased up to 100% of the S45C base metal under the condition of all heating time. Optimal welding conditions were n=2,000(rpm), P₁=60(MPa), P₂=100(MPa), t₁=4(s), t₂=5(s) when the total upset length is 5.4(㎜).
Seroprevalence of equine piroplasms in the Republic of Korea
Seo, M.G.,Yun, S.H.,Choi, S.K.,Cho, G.J.,Park, Y.S.,Kwon, O.D.,Cho, K.H.,Kim, T.H.,Jeong, K.S.,Park, S.J.,Kwon, Y.S.,Kwak, D. Elsevier Scientific Pub. Co 2011 Veterinary parasitology Vol.179 No.1
Equine piroplasms include two tick-borne protozoan parasites, Babesia caballi and Theileria equi. Although no clinical equine piroplasmosis has been reported in the Republic of Korea, the possible existence of the disease has been proposed due to a nationwide distribution of the vector ticks. To determine if the antibodies against B. caballi and T. equi were present, 184 sera of horses (Equus caballus) raised in the Republic of Korea from 2007 to 2010 were assessed using cELISA kits. Two (1.1%) out of 184 sera were positive for T. equi, but none were seropositive for B. caballi. Both samples tested positive came from one region (Gyeonggi province). The accuracy of the cELISA was confirmed by PCR using primers specific to the 18S rRNA of T. equi. This study presents for the first time horses infected by T. equi in the Republic of Korea. Since the infection of T. equi occurred in horses raised in the Republic of Korea, further studies with continuous monitoring of the vector ticks for equine piroplasms and appropriate control programs need to be established.
Choo, Y.K.,Kwon, H.J.,Oh, S.T.,Kang, C.W.,Kim, H.K.,Hong, E.C.,Heo, K.N.,Lee, S.K.,An, B.K. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4
There are multiple experiments conducted with male Korean native ducks (KND) to evaluate the optimal levels of limiting amino acids (AA). In Exp. 1, a total of 450 one-d-old male KNDs were divided into five groups with six replicates and fed experimental diets with varying levels of lysine, total sulfur amino acids (TSAA) and threonine (T1, 0.90/0.74/0.70%; T2, 1.00/0.82/0.77%; T3, 1.10/0.90/0.85%; T4, 1.20/0.98/0.93%; T5, 1.30/1.07/1.01%) to 21 d of age. In Exp. 2, one-d-old male KND were received and fed commercial starter diet from hatching to 21 d of age, and then divided into five groups with six replicates and fed one of five diets varying levels of lysine, TSAA, and threonine (T1, 0.73/0.62/0.54%; T2, 0.80/0.68/0.60%; T3, 0.87/0.74/0.65%; T4, 0.94/0.80/0.70%; T5, 1.01/0.86/0.75%) during 22 to 56 d of age, respectively. The BW gain was linearly increased as dietary limiting AA levels increased to 1.20% lysine, 0.98% TSAA and 0.93% threonine. There were no significant differences in feed intake, gain:feed and uniformity among groups. In Exp. 2, the BW gain and gain:feed were not affected by dietary limiting AA levels. There were no significant differences in carcass characteristics and meat quality among groups. The growth performance and carcass characteristics did not show the significant response to increasing dietary limiting AA levels in KND during 22 to 56 d of age. In conclusion, the levels of lysine, TSAA and threonine necessary to maximize growth for starter phase were at least 1.20%, 0.98%, and 0.93%, respectively. On the other hands, KND require relatively low levels of limiting AA for late growth and carcass yield. The dietary levels of 0.73% lysine, 0.62% TSAA and 0.54% threonine appear to be adequate during growing phase.
Rapid and label-free bioanalytical method of alpha fetoprotein detection using LSPR chip
Kim, D.,Kim, J.,Kwak, C.H.,Heo, N.S.,Oh, S.Y.,Lee, H.,Lee, G.W.,Vilian, A.T.E.,Han, Y.K.,Kim, W.S.,Kim, G.b.,Kwon, S.,Huh, Y.S. North-Holland Pub. Co 2017 Journal of crystal growth Vol.469 No.-
Alpha fetoprotein (AFP) is a cancer marker, particularly for hepatocellular carcinoma. Normal levels of AFP are less than 20ng/mL; however, its levels can reach more than 400ng/mL in patients with HCC. Enzyme linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) have been employed for clinical diagnosis of AFP; however, these methods are time consuming and labor intensive. In this study, we developed a localized surface plasmon resonance (LSPR) based biosensor for simple and rapid detection of AFP. This biosensor consists of a UV-Vis spectrometer, a cuvette cell, and a biosensor chip nanopatterned with gold nanoparticles (AuNPs). In our LSPR biosensor, binding of AFP to the surface of the sensor chip led to an increasing magnitude of the LSPR signals, which was measured by an ultraviolet-visible (UV-Vis) spectrometer. Our LSPR biosensor showed sufficient detectability of AFP at concentrations of 1ng/mL to 1μg/mL. Moreover, the overall procedure for detection of AFP was completed within 20min. This biosensor could also be utilized for a point of care test (POCT) by employing a portable UV-Vis spectrometer. Owing to the simplicity and rapidity of the detection process, our LSPR biosensor is expected to replace traditional diagnostic methods for the early detection of diseases.
Systematic Investigation of Possibilities for New Physics Effects in b →s Penguin Processes
S. Kim, C.,J. Kwon, Y.,Lee, Jake,Yoshikawa, T. Yukawa Institute for Theoretical Physics and the P 2006 Progress of theoretical physics Vol.116 No.1
<P>Although recent experimental results in b →s penguin process seem to be roughly consistent with the standard model predictions, there may be still large possibilities of new physics hiding in these processes. Therefore, here we investigate systematically the potential new physics effects that may appear in time-dependent CP asymmetries of B →φK0, B →η′K0 and B →K0 π0 decay modes, by classifying the cases for the values of the mixing-induced indirect CP asymmetries, SφK0, Sη′K0, SK0 π0 which are compared to SJ/ψK0. We also show that several Bs decay modes may help to resolve the ambiguities in such an analysis. Through combining analysis with the time-dependent CP asymmetries of Bs decay modes such as Bs →φη′, Bs →η' π0 and Bs→K0 overlineK0, we can determine where the new CP phases precisely come from.</P>
Experimental Approach to Explosive Nucloeosynthesis with RI Beams
S. Kubono,Y. Yamaguchi,G. Amadio,S. Hayakawa,Y. Wakabayashi,Y. Kurihara,J. J. He,A. Saito,H. Fujikawa,Le Hong Khiem,M. Niikura,T. Teranishi,N. Iwasa,S. Kato,S. Nishimura,C. S. Lee,Y. K. Kwon,I. S. Hah 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.1
Experimental efforts to investigate stellar reactions under high-temperature and high density conditions have been made as a major program using the RI beams from the Center for Nuclear Study (CNS) low-energy in-flight RI beam separator (CRIB) at University of Tokyo in order to understand the evolution of the universe, as well as various stellar phenomena. Specically, two subjects of hydrogen burning are discussed here. One is a reaction study of the pp-chain and the second is of the explosive hydrogen burning, the rp-process. Some s-wave resonances have been identified by the thick target method in the crucial reaction processes in the hydrogen burning. The resonant scattering with the thick target method also succeeded in identifying inelastic resonant scattering, giving proton widths for the first excited state of the target nucleus. This provided very efficiently the reaction rate estimate for the process under high-temperature equilibrium conditions. Possibilities of the CRIB facility in near future are also briey discussed. Experimental efforts to investigate stellar reactions under high-temperature and high density conditions have been made as a major program using the RI beams from the Center for Nuclear Study (CNS) low-energy in-flight RI beam separator (CRIB) at University of Tokyo in order to understand the evolution of the universe, as well as various stellar phenomena. Specically, two subjects of hydrogen burning are discussed here. One is a reaction study of the pp-chain and the second is of the explosive hydrogen burning, the rp-process. Some s-wave resonances have been identified by the thick target method in the crucial reaction processes in the hydrogen burning. The resonant scattering with the thick target method also succeeded in identifying inelastic resonant scattering, giving proton widths for the first excited state of the target nucleus. This provided very efficiently the reaction rate estimate for the process under high-temperature equilibrium conditions. Possibilities of the CRIB facility in near future are also briey discussed.
Kwon, K.M.,Kang, S.G.,Sokolova, T.G.,Cho, S.S.,Kim, Y.J.,Kim, C.H.,Kwon, S.T. IPC Science and Technology Press ; Elsevier Scienc 2016 Enzyme and microbial technology Vol.86 No.-
<P>The family B DNA polymerase gene from the euryarchaeon Thermococcus barophilus Ch5 (Tba5) contains an open reading frame of 6198 base pairs that encodes 2065 amino acid residues. The gene is split by three inteins that must be spliced out to form the mature DNA polymerase. A Tba5 DNA polymerase gene without inteins (genetically intein-spliced) was expressed under the control of the pET-28b(+)T7lac promoter in E. coli Rosetta 2(DE3)pLysS cells. The molecular mass of the purified Tba5 DNA polymerase was about 90 kDa consistent with the 90,470 Da molecular mass calculated based on the 776 amino acid sequence. The optimal pH for Tba5 DNA polymerase activity was 7.5 and the optimal temperature was 70-75 degrees C. The enzyme possessed 3' -> 5' exonuclease activity and was activated by magnesium ions. PCR amplification using Tba5 DNA polymerase enables high-yield for 1- to 6-kb target DNA products, while 8- to 10-kb target DNA products were amplified at low or inefficient levels. To simultaneously improve product yield and amplification fidelity, Tba5 plus DNA polymerase mixtures were constituted with various amounts of Tba5 DNA polymerase mixed with Taq DNA polymerase. The Tba5 plus DNA polymerase mixtures robustly amplified up to 25-kb lambda DNA fragments. In addition, the PCR error rate of Tba5 plus3 and Tba5 plus4 mixtures were much lower than those of wild-type Tba5 DNA polymerase, Pfu DNA polymerase, Taq DNA polymerase, and Pfu plus DNA polymerase. (C) 2016 Elsevier Inc. All rights reserved.</P>
Kwon, Y.T.,Park, M.S.,Kim, J.D.,Choi, K.Y.,Jung, M.H.,Iyo, A.,Tokiwa, K.,Kim, Y.C.,Lee, S.I. Elsevier 2010 CURRENT APPLIED PHYSICS Vol.10 No.4
By using the Hao-Clem model, we analyzed the equilibrium magnetization of the grain-aligned HgBa<SUB>2</SUB>Ca<SUB>4</SUB>Cu<SUB>5</SUB>O<SUB>12+y</SUB> (Hg1245) with T<SUB>c</SUB>~108K. We obtained thermodynamic parameters, such as the penetration depth [λ<SUB>ab</SUB>(0)] and the coherence length [ξ<SUB>ab</SUB>(0)] from the thermodynamic critical field [H<SUB>c</SUB>] and the Ginzburg-Landau parameter [κ]. Compared to the four layered superconductor HgBa<SUB>2</SUB>Ca<SUB>3</SUB>Cu<SUB>4</SUB>O<SUB>10+y</SUB> (Hg1234), the obtained penetration depth was slightly increased indicating that the Cooper pair density of Hg1245 was decreased, which was one of the reasons why the transition temperature (T<SUB>c</SUB>) of the Hg1245 did not increase as compared to the Hg1234. The expected increase of T<SUB>c</SUB> for the material with the more CuO<SUB>2</SUB> planes was not achieved because of the insufficient doping of the Cooper pairs while adding CuO<SUB>2</SUB> planes.