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- 저자 : Roman Bleha (검색결과 2 건)
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Sarang Cho,Roman Bleha,Jeong Yeon Seo,Jisun Lee,Chang Won Lee,Andriy Synytsya,Yong Il Park 한국당과학회 2017 한국당과학회 학술대회 Vol.2017 No.01
Polysaccharides of Korean pine nut were isolated, identified and their bioactivity was evaluated. The water-soluble polysaccharides were extracted from the defatted Korean pine nut by boiling for 3 h in water and purified by ethanol precipitation and DEAE-Cellulose column chromatography. The monosaccharide composition analysis showed that purified polysaccharide(s) (PNE-P1) consisted of xylose (7.2, molar ratio), arabinose (8.8), and galacturonic acid (4.4) as major sugars and other minor sugars including glucose, galactose, rhamnose, and glucuronic acid. Their molecular weights were determined to be 1,213 kDa, 634 kDa and 331 kDa by size-exclusion HPLC. FT-IR and proton NMR analyses showed that PNE-P1 is a mixture of pectin-type polysaccharides containing arabinan, homogalacturonan (HG), rhamnogalacturonan type 1 (RG-1) and heteroxylan. The immunostimulating activity of PNE-P1 was investigated in murine macrophage RAW264.7 cells. PNE-P1 did not show any detectable level of cytotoxicity to RAW264.7 cells in the concentration range tested (10-200 μg/ml) and rather, it promoted the cell proliferation. PNE-P1 induced the production of pro-inflammatory cytokines (TNF-α, Interleukin-6 and Interleukin-10) and chemokines (RANTES and MIP-1α). These results indicate that PNE-P1 stimulated the RAW264.7 cells to increase cytokine secretions. Taken together, these results suggest that PNE-P1 can be considered as a potential immunopotentiator.
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Sarang Cho,Peter Capek,Roman Bleha,Andrej Sinica,Jung Sik Lim,Jin Ree,Yong Il Park 한국당과학회 2022 한국당과학회 학술대회 Vol.2022 No.07
Many plant polysaccharide have immunomodulatory and anti-tumour activities. In the present study, water-soluble polysaccharides were isolated from an edible plant seed mainly by hot water extraction, 75% ethanol precipitation, and DEAE-cellulose column chromatography and tentatively named as PSP-F1. The PSP-F1 was shown to be a mixture of three major polysaccharides with molecular mass of approximately 1,200, 640, and 300 kDa, respectively, as estimated by size-exclusion HPLC analysis. The HPAEC-PAD analysis for neutral sugar composition and showed that PSP-F1 consists of arabinose (Ara) and xylose (Xyl), as the major neutral sugars, and other minor neutral sugars such as rhamnose (Rha), galactose (Gal), and glucose (Glc). HPLC analysis for uronic acids showed that galacturonic acid (GalA) presents as the major uronic acid and glucuronic acid (GlcA) was the minor one. Together with the neutral monosaccharides and uronic acids composition, the FT-IR and NMR analysis suggested that PSP-F1 appears to be a mixture of pectin type and heteroxylan polysaccharides. The results showed that the PSP-F1 (0-100 μg/ml) significantly induced the secretion of pro-inflammatory cytokines TNF-α and IL-6 and upregulation of MAPKs (ERK, JNK and p38) phosphorylation. The secretion of various pro-inflammatory cytokines from macrophages is mainly caused by MAPK cascade activation. These results demonstrated that the PSP-F1 isolated from a plant seed is a mixture of pectin type and heteroxylan polysaccharides, which are typical for plant cell wall polysaccharides, and that it effectively stimulates macrophages to secrete pro-inflammatory cytokines, such as TNF-α and IL-6, by upregulation of MAPKs phosphorylation. The results of this study suggests that PSP-F1 may be potential immunostimulator for health benefits and as a pharmaceutical component.
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