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Rina Hidayati Pratiwi,Iman Hidayat,Muhammad Hanafi,Wibowo Mangunwardoyo 한국미생물학회 2017 The journal of microbiology Vol.55 No.4
This study’s aim was to determine the identity of antibacterialcompounds produced by Pseudomonas aeruginosa strainUICC B-40 and describe the antibacterial compounds’ mechanismsof action for damaging pathogenic bacteria cells. Isolation and identification of the compounds were carriedout using thin layer chromatography (TLC), nuclear magneticresonance (NMR) spectroscopy and liquid chromatographymass spectrometry (LC-MS) analyses. Antibacterialactivity was assayed via minimum inhibitory concentration(MIC) and the antibacterial compound mechanism was observedmorphologically through scanning electron microscopy(SEM). This study successfully identified the (2E,5E)-phenyltetradeca-2,5-dienoate antibacterial compound (molecularweight 300 g/mol), composed of a phenolic ester, fattyacid and long chain of aliphatic group structures. MIC valuesfor this compound were determined at 62.5 μg/ml againstStaphylococcus aureus strain ATCC 25923. The mechanismof the compound involved breaking down the bacterial cellwalls through the lysis process. The (2E,5E)-phenyltetradeca-2,5-dienoate compound exhibited inhibitory activity on thegrowth of Gram-positive bacteria.