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Development of male sterile transgenic lines in rice by tapetum specific expression of barnase gene
Kumar, Pravin,Kaur, Kulwinder,Purty, Ram Singh,Mohan, Madan,Burma, Pradeep Kumar The Korean Society of Plant Biotechnology 2017 식물생명공학회지 Vol.44 No.4
The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.
Pravin Kumar,Ram Singh Purty 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.5
The indispensable requirement for the barnase–barstar based hybrid seed production technology is the availability of a tightly regulated tapetum-specifc promoter. Until now, the specifcity of literature reported tapetum-specifc promoters are mainly characterized based on the fusion of non-lethal GUS/GFP gene as promoter–reporter fusion approach. In our previous report, the tapetum-specifc OsG6b promoter had successfully generated 100% male sterile barnase line, whereas the OsRTS promoter (from indica rice cultivar IR64) was leaky in nature. In continuation, our observation with another two tapetum-specifc promoters is reported here. Two distinct phenotypes with no male sterility were observed in the OsFbox promoter barnase lines due to leaky expression, whereas almost half of the OsYY2 promoter lines were male sterile. Based on our results, it is clear that the tapetum-specifc promoters characterized based on the fusion of non-lethal GUS/GFP gene regulate the non-specifc lethal barnase gene diferently. Therefore, the ideal way of characterizing the tapetum-specifc promoter for hybrid seed production should be a lethal gene such as barnase instead of non-lethal GUS/GFP gene
Development of male sterile transgenic lines in rice by tapetum specific expression of barnase gene
Pravin Kumar,Kulwinder Kaur,Ram Singh Purty,Madan Mohan,Pradeep Kumar Burma 한국식물생명공학회 2017 JOURNAL OF PLANT BIOTECHNOLOGY Vol.44 No.4
The key to development of barnase-barstar transgene based hybrid seed technology is the availability of tightly regulated tapetum specific promoter, as any leaky expression of the barnase gene leads to several unintended effects. In the present study, we used two different tapetum specific promoters i.e. promoter of the RTS gene isolated from rice cultivar IR64 and the OsG6b promoter from japonica rice cultivar Hayayuki to express the barnase gene in rice transgenic lines. While viable male sterile transgenic lines could not be obtained with RTS promoter we could develop single copy male sterile lines when the barnase gene was expressed under the OsG6b promoter.
Bhati, Tushar,Gupta, Rahul,Yadav, Nisha,Singh, Ruhi,Fuloria, Antra,Waziri, Aafrin,Chatterjee, Sayan,Purty, Ram Singh The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.2
In the present study, we have studied the bioremediating capability of bacterial strain against six heavy metals. The strain was isolated from river Yamuna, New Delhi which is a very rich repository of bioremediating flora and fauna. The strain was found to be Gram positive as indicated by Gram staining. The strain was characterized using 16s rRNA gene sequencing and the BlastN result showed its close resemblance with the Cellulosimicrobium sp. As each treatment has its own toxicity eliciting expression of different factors, we observed varied growth characteristics of the bacterial isolate and its protein content in response to different heavy metals. The assessment of its bioremediation capability showed that the strain Cellulosimicrobium sp. has potential to consume or sequester the six heavy metals in this study in the following order iron > lead > zinc > cooper > nickel > cadmium. Thus, the strain Cellulosimicrobium sp. isolated in the present study can be a good model system to understand the molecular mechanism behind its bioremediating capabilities under multiple stress conditions.
Tushar Bhati,Rahul Gupta,Nisha Yadav,Ruhi Singh,Antra Fuloria,Aafrin Waziri,Sayan Chatterjee,Ram Singh Purty 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2
In the present study, we have studied the bioremediating capability of bacterial strain against six heavy metals. The strain was isolated from river Yamuna, New Delhi which is a very rich repository of bioremediating flora and fauna. The strain was found to be Gram positive as indicated by Gram staining. The strain was characterized using 16s rRNA gene sequencing and the BlastN result showed its close resemblance with the Cellulosimicrobium sp. As each treatment has its own toxicity eliciting expression of different factors, we observed varied growth characteristics of the bacterial isolate and its protein content in response to different heavy metals. The assessment of its bioremediation capability showed that the strain Cellulosimicrobium sp. has potential to consume or sequester the six heavy metals in this study in the following order iron > lead > zinc > cooper > nickel > cadmium. Thus, the strain Cellulosimicrobium sp. isolated in the present study can be a good model system to understand the molecular mechanism behind its bioremediating capabilities under multiple stress conditions.