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부위특이 재조합을 이용한 생쥐 Embryonic Stem 세포에서의 유청단백질 유전자의 불활성화
이창규,Piedrahita, Jorege A. 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.6
Regulatory elements of the whey acidic protein (WAP) gene are widely used for the expression of transgenes into milk. Although high levels of transgene expression can be obtained using the WAP promoter, there can be problems in these transgenic animals such as abnormal development of the mammary gland. Towards the understanding of its function, the mouse WAP gene in embryonic stem (ES) cells was inactivated using a combination of homologous recombination and site-specific recombination. The endogenous locus was successfully targeted with a construct containing three loxP sites. After transient expression of Cre recombinase, two types of deletion events were seen in the targeted WAP locus, a complete deletion of the WAP gene and the neomycin-thymidine kinase (Neo-TK) selection cassette (type I deletion), and a deletion of the selection cassette only leaving the intact WAP gene flanked by two loxP sites (type II deletion). Both of these cell lines should facilitate the study of the physiological function of the WAP.
Lim, Ji-Hey,Piedrahita, Jorge A.,Jackson, Lauren,Ghashghaei, Troy,Olby, Natasha J. Mary Ann Liebert 2010 Cellular reprogramming Vol.12 No.6
<P>Abstract Research into transplantation strategies to treat spinal cord injury (SCI) is frequently performed in rodents, but translation of results to clinical patients can be poor and a large mammalian model of severe SCI is needed. The pig has been considered an optimal model species in which to perform preclinical testing, and the Yucatan minipig can be cloned successfully utilizing somatic cell nuclear transfer (SCNT). However, induction of paralysis in pigs poses significant welfare and nursing challenges. The present study was conducted to determine whether Yucatan SCNT clones could be used to develop an SCI animal model for cellular transplantation research. First, we demonstrated that transection of the sacrocaudal spinal cord in Yucatan SCNT clones produces profound, quantifiable neurological deficits restricted to the tail. We then established that neurospheres could be isolated from brain tissue of green fluorescence protein (GFP) transfected SCNT clones. Finally, we confirmed survival of transplanted GFP-expressing neural stem cells in the SCI lesion and their differentiation into glial and neuronal lineages for up to 4 weeks without immunosuppression. We conclude that this model of sacrocaudal SCI in Yucatan SCNT clones represents a powerful research tool to investigate the effect of cellular transplantation on axonal regeneration and functional recovery.</P>
Transgenesis and Germ Cell Engineering in Domestic Animals
Lee, C.K.,Piedrahita, J.A. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.6
Transgenesis is a very powerful tool not only to help understanding the basics of life science but also to improve the efficiency of animal production. Since the first transgenic mouse was born in 1980, rapid development and wide application of this technique have been made in laboratory animals as well as in domestic animals. Although pronuclear injection is the most widely used method and nuclear transfer using somatic cells broadens the choice of making transgenic domestic animals, the demand for precise manipulation of the genome leads to the utilization of gene targeting. To make this technique possible, a pluripotent embryonic cell line such as embryonic stem (ES) cell is required to carry genetic mutation to further generations. However, ES cell, well established in mice, is not available in domestic animals even though many attempt to establish the cell line. An alternate source of pluripotent cells is embryonic germ (EG) cells derived from primordial germ cells (PGCs). To make gene targeting feasible in this cell line, a better culture system would help to minimize the unnecessary loss of cells in vitro. In this review, general methods to produce transgenic domestic animals will be mentioned. Also, it will focus on germ cell engineering and methods to improve the establishment of pluripotent embryonic cell lines in domestic animals.
Multidimensional Fourier transform and fractional derivative
J.E. RESTREPO,A. PIEDRAHITA,P. AGARWAL 장전수학회 2019 Proceedings of the Jangjeon mathematical society Vol.22 No.2
Some properties of the multidimensional Fourier transform and the suitable fractional derivative are established. The obtained results are used to prove that the inverse problem for a time fractional advection-dispersion equation in a 2{D setting is ill-posed.
Application of Animal Biotechnology to the Beef Industry
Westhusin, M.E.,Piedrahita, J.A. 韓國受精卵移植學會 1995 한국동물생명공학회지 Vol.10 No.1
In conclusion, tremendous potential exists for the application of animal biotechnology to the beef industry, especially with the utilization of embryo cloning to produce genetically identical animals and genetic engineering to modify animal genomes to improve and /or create new phenotypes for many economically important traits. Research involving embryo cloning and genetic engineering of animals has been continuous now for over a decade, however inefficiencies in techniques have prevented large scale application. large numbers of identical cattle will some day be produced and producers will be utilizing transgenic cattle in their beef production programs.
Lee Chang-Kyu,Jorge A. Piedrahita 한국발생생물학회 2001 한국발생생물학회 학술발표대회 Vol.2001 No.-
One of the problems associated with in vitro culture of primordial gern cells (PGCs) is the large loss of cells during the initial period of culture. This study characterized the initial loss and determined the effectiveness of two classes of apoptosis inhibitors, protease inhibitors and antioxidants, on the ability of the porcine PGCs to survive in culture. Results from electron microscopic analysis and in situ DNA fragmentation assay indicated that porcine PGCs rapidly undergo apoptosis when placed in culture. Additionally, \ulcorner2-macroglobulin, a protease inhibitor and cytokine carrier, and N-acetylcysteine, an antioxidant, increased the survival of PGCs in vitro. While other protease inhibitors tested did not affect survival of PGCs, all antioxidants tested improved survival of PGCs (p<0.05). Further results indicated that the beneficial effect of the antioxidants was critical only during the initial period of culture. Finally, it was determined that in short-term culture, in the absence of feeder layer, antioxidants could partially replace the effect(s) of growth factors and reduce apoptosis. Collectively, these results indicate that the addition of \ulcorner2-macroglobulin and antioxidatns can increase the number of PGCs in vitro by suppressing apoptosis.
J. P. Morán-Lázaro,F. López-Urías,E. Muñoz-Sandoval,M. Courel-Piedrahita,A. Carreon-Alvarez,V. M. Rodríguez-Betancourtt,I. Zamudio-Torres,E. S. Guillén-López,A. Palafox-Corona 대한금속·재료학회 2023 ELECTRONIC MATERIALS LETTERS Vol.19 No.1
The acetone contained in human breath is of great interest for the health sector as it is a marker that allows to diagnoseand control diabetes in a non-invasive way. However, its concentration is extremely low. Therefore, high-performanceacetone sensors are still a challenge. With this in mind, MgCo 2 O 4 nanoparticles were synthesized using a microwaveassistedcolloidal route with subsequent calcination. Structural and morphological characterizations were done through varioustechniques. The MgCo 2 O 4 sensor was fabricated with the sample calcined at 500 °C. The sensing results showed that thesensor could detect acetone vapors ranging from 0.5 to 50 ppm at an optimum operating temperature of 250 °C with a highresponse, repeatability, stability, and selectivity. These sensing characteristics revealed that MgCo 2 O 4 could be used as a newsensor material to detect acetone in exhaled human breath.