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이광원,박문희,오신규,한정민 한국안전학회 2003 한국안전학회지 Vol.18 No.4
Bayes theorem, suggested by the British Mathematician Bayes (18th century), enables the prior estimate of the probability of an event under the condition given by a specific event. This theorem has been frequently used to revise the failure probability of a component or system. 2-stage Bayesian procedure was firstly published by Shultis et al. (1981) and Kaplan (1983), and was further developed based on the studies of Hora & Iman (1990) Papazpgolou et al., P?n(1993). For a small observed failure number (below 12), the estimated reliability of a system or component is not reliable. In the case in which the reliability data of the corresponding system or component can be found in a generic reliability reference book, however, a reliable estimation of the failure probability can be realized by using Bayes theorem, which jointly makes use of the observed data (specific data) and the data found in reference book (generic data).
거북밀깍지벌레(Cerostegia japonicus)의 發生消長과 그 防除에 관한 硏究
裵泰雄,曺種澤,河祥永,韓熙世,文炳周 東亞大學校 1979 東亞論叢 Vol.16 No.2
거북밀깍지벌레의 效果的인 防除法을 규명하기 위하여 釜山地域에서의 發生消長을 검토하고, 4종류의 寄主別로 기계유유제, 有機燐劑 3종 및 Carbamate劑 3종을 공시하여 殺蟲率을 Bliss의 Probit법으로 분석하고 農藥의 농도에 따른 蟲體의 呼吸代謝를 검토 하였다. 1. 釜山地方에서의 거북밀깍지벌레의 孵化器는 6月15日∼7月12日이였으며 孵化最盛期는 7月 初旬이였다. 2. 防除適期는 8月10日경이었다. 3. 8月3日에 처리한 寄主別의 본 害蟲의 LD?이 가장 낮은 것은 자목련에서는 DEP, 감나무에서는 BUX,사철나무와 치자나무에서는 BUX와 DEP로서 전체적으로 볼 때 BUX 및 DEP가 가장 效果的이고 그 다음이 Diazinon이였다. 4. 8月18日 처리에서 LD?이 가장 낮은 것은 자목련과 감나무에서는 Diazinon이, 사철나무와 치자나무에서는 BUX와 DEP로서 전체적으로 보면 Diazinon, BUX와 DEP등이 感受性이 높았다. 5. 8月3日의 처리에서 LD?이 가장 낮은 농약은 BUX로서 寄主別 농도 범위는 0.011∼0.043%였다. 6. 8月18日의 처리에서 LD?이 가장 낮은 농약은 BUX와 Diazinon으로서 寄主別 농도 범위는 각각 0.011∼0.1076%, 0.028∼0.074%였다. 7. 本 害蟲의 正常 酸素消費量은 9.25∼10.02㎕O₂/㎎ body weight/hour였으며 寄主에 따른 차이를 인정할 수 없었다. 8. Diazinon과 BUX에서는 농도가 높아질수록 산소소비량은 정상호흡량 보다 훨씬 증가하였으나 기계유유제에서는 감소되었다. In order to investigate the effective control of Florida was scale (Cerostegia japonicus), the seasonal occurrences of 1st in star in Busan area was examined, the insecticides tested with the different four hosts were carbamates(NAC,BUX BPMC), organicphosphates(DEP,MEP,Diazinon)and Machine-oil emulson, and the mortality was analyzed by the probit analysis. Also the effects of insecticide's concentration on respiratory metabolism of Florida wax scale was examined. The results obtained were as follows 1. The periods of the larva occurrence was from June 15 to July 12, and the major peak of its occurrence was found to be in the early July. 2. The adequate period for the effective control of Florida wax scale was about August 10. 3. The lowest insecticides of LD 50 values to Florida wax scale with each hosts in August 10 was DEP in the people magnolia, BUX in the persimmon tree, BUX and DEP in the spindle tree and the gardenia. So generally the most effective insecticides for controlling it were BUX and DEP, Diazinon was the next. 4. In the second experiments treated in August 18, the lowest insecticide of LD50 values to it was Diazinon in the purple magnolia and the persimmon tree, BUX and DEP in the spindle tree and the gardenia. So the susceptibility of it to the insecticides was highest in Diazinon, BUX and DEP. 5. The lowest insecticide of LD90 values to it was BUX and the concentration range by the hosts was from. 0.011% to 0.043%. 6. In the second experiment treated in August 18, the lowest insecticides of LD90 values to it were BUX and Diazinon, and the concentration ranges by the hosts were from 0.011 to 0.1076%, 0.028 to 0.074% respectively. 7. The normal oxygen consumption of Florida wax scale was from 9.25 to 10.02㎕O₂/㎎ body weight/hour and wasn't recognized the significance by the hosts. 8. The oxygen consumption of it in Diazinon and BUX was higher with increasing of insecticides concentration than the normal respiratory, but it was decreased in machine oil emulsion.
Moon, Hi Gyu,Jung, Youngmo,Han, Soo Deok,Shim, Young-Seok,Jung, Woo-Suk,Lee, Taikjin,Lee, Seok,Park, Jung Han,Baek, Seung-Hyub,Kim, Jin-Sang,Park, Hyung-Ho,Kim, Chulki,Kang, Chong-Yun Elsevier 2018 Sensors and actuators. B Chemical Vol.257 No.-
<P><B>Abstract</B></P> <P>Chemiresistive electronic nose (CEN) composed of villi-like nanostructures (VLNs) of SnO<SUB>2</SUB> and WO<SUB>3</SUB>, and Au-functionalized VLNs was fabricated by applying electron-beam evaporation in a glancing angle deposition mode. The VLNs-based CEN with a back-heater (212°C) shows high responses with low detection limits of parts per billion (ppb)-levels for NO and NH<SUB>3</SUB> vapors at 80% relative humidity atmosphere. The enhanced sensitivities in a high humidity condition turn out to be attributed to the spillover effect by the Au nanoparticles and a large surface-to-volume ratio in porous VLNs. Employing Au NPs on VLNs leads to the increase of O<SUP>−</SUP> ions via the spillover effect which impedes the adsorption of water molecules, maintaining the enhanced responses against environmental humidity. Consequently, high responses for NO and NH<SUB>3</SUB> vapors maintain even in the high humidity condition. Herein, with the principal component analysis (PCA), we demonstrate highly selective detection of NO and NH<SUB>3</SUB> vapors against C<SUB>2</SUB>H<SUB>5</SUB>OH, CO, C<SUB>7</SUB>H<SUB>8</SUB>, C6H6, and CH<SUB>3</SUB>COCH<SUB>3</SUB> vapors. These results open up wide applications of the VLNs-based CEN as an inexpensive and non-invasive diagnostic tool for asthma and kidney disorder.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A villi-like nanostructures (VLNs)-based chemiresistive electronic nose (CEN) as exhaled breath analyzer was developed. </LI> <LI> The VLNs-based CEN exhibits high sensitive detection to NO (Asthma) and NH<SUB>3</SUB> (Kidney disorder) as biomarkers. </LI> <LI> The detection limits (DLs) of the VLNs-based CEN are 899ppt–10.2ppb for NO and 312ppb–639ppb for NH<SUB>3</SUB>, respectively. </LI> <LI> The enhanced responses in humid environment are attribute to the spillover effect by Au nanoparticles. </LI> <LI> The highly selective NO and NH<SUB>3</SUB> detections were clearly distinguished by principal component analysis (PCA). </LI> </UL> </P>
Development of a Competitive Enzyme-Linked Immunoassay for Human Apolipoprotein B
Han,Moon Hi,Kwak,Ju Won,Yoon,Michung 한국지질학회 1993 韓國脂質學會誌 Vol.3 No.1
Apolipoprotein (apo) B, the protein moiety of low density lipoprotein (LDL), is known as a better marker for prediction of coronary artery disease than is the customary lipid marker, LDL cholesterol. We developed a competitive enzyme-linked immunosorbent assay (ELISA) to measure this apo B concentration in human serum. 96-well microtiter plates were coated with native LDL. After washing the unbound LDL and blocking non-specific binding sites, antiserum to apo B prepared in rabbits was added to the wells together with serum samples. After the incubation and washing steps, bound antibodies were measured by addition of horseradish peroxidase-conjugated goat anti-rabbit IgG and relevant substrates of peroxidase. Standard curve was obtained by using freshly prepared LDL-apo B standard. The sensitivity of assay was 0.125 ㎍ of apo B per ㎖ of human serum. About cross-reactivity, human serum albumin nor apo A-I didn't show any detectable cross-reaction with the antiserum against apo B in the assay system. The within-assay coefficient of variance (CV) was 4.5% and the between-assay CV was 8.9%. For validation of the assay, 35 serum samples with known LDL cholesterol values were used for the apo B assay, and the measured apo B values correlated with LDL cholesterol values to a correlation coefficient (γ) of 0.79. From these results, it is concluded that the assay has several advantages; sensitivity, specificity, no need of hazardous radiochemicals and potential for use with monoclonal antibodies.