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Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng
Fan, Ze-Yan,Miao, Cui-Ping,Qiao, Xin-Guo,Zheng, You-Kun,Chen, Hua-Hong,Chen, You-Wei,Xu, Li-Hua,Zhao, Li-Xing,Guan, Hui-Lin The Korean Society of Ginseng 2016 Journal of Ginseng Research Vol.40 No.2
Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.
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Miao Xue,Yanqin Wu,Wenzhe Fan,Jian Guo,Jialiang Wei,Hongyu Wang,Jizhou Tan,Yu Wang,Wang Yao,Yue Zhao,Jiaping Li 대한암학회 2020 Cancer Research and Treatment Vol.52 No.3
Purpose This study aimed to investigate the clinicopathologic features and mutational landscape of patients with hepatitis B virus (HBV)–related advanced hepatocellular carcinomas (HCC) undergoing transcatheter arterial chemoembolization (TACE). Materials and Methods From January 2017 to December 2018, 38 patients newly diagnosed with HBV-related advanced HCC were enrolled in the final analysis. Their pathological tissues and corresponding blood samples before TACE treatment were collected for whole-exome sequencing. Response to TACE was evaluated at 1-3 months after two consecutive use of TACE. Predictive factors were analyzed by univariate and multivariate analyses in a bivariate Logistic regression model. Enrichment of related pathways of all driver genes were acquired using the gene set enrichment analysis (GSEA). Results Among 38 patients, 23 (60.5%) exhibited TACE failure/refractoriness. Patients with TACE failure/refractoriness showed higher frequency of TP53 mutation than their counterparts (p=0.020). Univariate and multivariate analyses showed that only vascular invasion and TP53mutation were significantly correlated with TACE failure/refractoriness in HBV-related advanced HCC. Of the 16 patients without vascular invasion, eight (50.0%) had TP53 mutations, and TP53mutation was associated with TACE failure/refractoriness (p=0.041). Moreover, GSEA showed that mitogen-activated protein kinase and apoptosis pathways induced by TP53 mutation were possibly associated with TACE failure/refractoriness. Conclusion Our study suggested that TP53 mutation was independently related with TACE efficacy, which may work via mitogen-activated protein kinase and apoptosis pathways. These findings may provide evidence to help distinguish patients who will particularly benefit from TACE from those who require more personalized therapeutic regimens and rigorous surveillance in HBV-related advanced HCC.
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Fan, M.,Yu, W.,Wang, W.,Guo, X.,Jin, K.,Miao, R.,Hou, W.,Kim, N.,Tao, J. Springer Science + Business Media 2017 JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE Vol.26 No.1
<P>Thin-multilayer Ti/Al laminates were prepared by one-step explosive bonding method to investigate the interface bonding and mechanical properties owing to their potential application in aerospace and auto industry. It was found that Ti/Al laminates prepared at the detonation velocity of 2100 m/s exhibited superior surface quality without any defects in comparison with other detonation velocities owing to the optimum explosive pressure and impact velocity. The interface morphologies and element distribution of Ti/Al laminates prepared at the detonation velocity of 2100 m/s were characterized by means of SEM and EDS. The results indicated that the linear and wavy bonding interface coexisted and element diffusion occurred in the interfacial zone. Furthermore, thin-multilayer Ti/Al laminates possessed high microhardness, excellent interlaminar shear and tensile properties.</P>
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Peng Miao,Fan Yang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.9
A continuous finite-time neural network with bias noises is proposed to solve the convex quadratic bilevel programming problem in this paper. In order to solve the convex quadratic bilevel programming problem, it is transformed into a nonlinear programming problem based on the Kaeush-Kuhn-Tucker conditions. Then, a neural network is designed to solve this problem. Compared with the existing networks, the designed network contains biased noise. Furthermore, it is proved that the proposed neural network can converge to the equilibrium point in finite time and it is Lyapunov stable. Moreover, the robustness performance of the present neural network against bias noises is discussed and the effect is very good. At the same time, the upper bound of the steady-state error is estimated. Lastly, two numerical examples show the effectiveness of the proposed methods.
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Peng Miao,Liujun Fan,Daoyuan Zhang 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.4
Based on the second order differential equation, this paper investigates finite-time stability, finite-timeconvergence criterions and estimates of convergence time. The main contributions of this paper lie in the factthat two new finite-time convergence criterions are proposed through the property of the second order differentialequation and their upper bound of the convergence time is derived. In addition, our finite-time stability criterionsare used to a recurrent neural network for solving time-varying Sylvester equation and Pseudo-Inverse of a Matrix. At last, a numerical example and a Pseudo-Inverse of a Matrix demonstrate the effectiveness of our method.
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Dynamic Characteristics of Saturated Soft Clays under Cyclic Loading in Drained Condition
Yong-hong Miao,Ruo-Yu Sheng,Jie Yin,Fan-Bo Zhou,Jian-fei Lu 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.2
This study presents an experimental investigation on the effects of cyclic loading waveform and cyclic stress ratio (CSR) on dynamic behaviors through laboratory tests on a source of soft clay under cyclic loading in drained condition. Test results showed that under different cyclic loading waveforms at a given frequency (f = 1 Hz) and CSR = 0.6, the axial strain increases significantly at a relatively lower number of cycles (N) and then became stable, while the axial strain consistently decreases with the increasing N and tend to be stable. At a given N, specimen under sine wave cyclic loads shows a higher axial strain rate than that under triangle wave or square cyclic loads. Test results also indicated that at different CSRs, the axial strain increases rapidly at a relatively lower N and then gradually tends to be stable with the increasing N at lower CSR yet there is no stable trend for specimens at higher CSR, which indicates there is a critical value of CSR. It is recommended to apply the cyclic loading in sine waveform with CSR close to and less than the critical value to get a better treatment effect.
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Wu Zhihao,Miao Wen,Yang Yuhan,Fan Gongjian,Wu Caie,Li Tingting,Xie Chunyan,Shen Dongbei 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.6
In this study, a high monacolin K yield was achieved through solid-state fermentation of Ginkgo biloba seeds. Monascus purpureus suspension made from red yeast rice was used as spore inoculum. Fermentation conditions in solid-state fermentation were optimized using response surface methodology, and the optimal conditions for the maximum monacolin K yield (17.71 ± 1.57 mg/g) were 0.22% ammonium sulfate, 0.34% ammonium chloride, 0.05% magnesium sulfate, fermentation time of 12 days, inoculation volume of 11%, and temperature of 27 °C. The total phenolic content of Monascus-fermented ginkgo seeds attained 9.67 mg GAE/g, 4.88-fold higher than that of unfermented ginkgo seeds. The scavenging abilities of DPPH and ABTS free radicals increased to 9.79 mg TE/g and 13.92 mg TE/g, respectively. These findings highlight the importance of investigating the optimal fermentation conditions for maximum monacolin K yield and the utilization value of ginkgo seed as fermentation substrate for higher bioactivities.
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Wei Dong,Miao Zhao,Fan-Long Jin,박수진 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7
Graphene nanoplatelets (GNPs) employed as conductive fillers were added to an epoxy matrix, diglycidyletherof bisphenol-A (DGEBA), to enhance the electrical conductivity and electromagnetic shielding efficiency ofDGEBA. In addition, we investigated the influence of GNP fraction on the thermal properties, flexural strength, impactstrength, electrical conductivity, electromagnetic shielding efficiency, and morphology of DGEBA/GNP nanocomposites. The electrical properties indicated that the electrical conductivity of DGEBA/GNP nanocomposites consisting ofhigher than 7.5 wt% GNPs was significantly improved compared to that of pristine DGEBA. The electromagneticshielding efficiency of the nanocomposite showed a maximum value of 3.87 dB at 10 wt% GNPs, which is 287% higherthan that of pristine DGEBA. An analysis of the fracture surfaces of the nanocomposites showed a rough morphologywith numerous micro-cracks. In addition, the GNPs in the DGEBA matrix were stacked and formed a continuousconductive path at high GNP contents.
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Diversity, distribution, and antagonistic activities of rhizobacteria of Panax notoginseng
Ze-Yan Fan,Cui-Ping Miao,Xin-Guo Qiao,You-Kun Zheng,Hua-Hong Chen,You-Wei Chen,Li-Hua Xu,Li-Xing Zhao,Hui-Lin Guan 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.2
Background: Rhizobacteria play an important role in plant defense and could be promising sources of biocontrol agents. This study aimed to screen antagonistic bacteria and develop a biocontrol system for root rot complex of Panax notoginseng. Methods: Pure-culture methods were used to isolate bacteria from the rhizosphere soil of notoginseng plants. The identification of isolates was based on the analysis of 16S ribosomal RNA (rRNA) sequences. Results: A total of 279 bacteria were obtained from rhizosphere soils of healthy and root-rot notoginseng plants, and uncultivated soil. Among all the isolates, 88 showed antagonistic activity to at least one of three phytopathogenic fungi, Fusarium oxysporum, Fusarium solani, and Phoma herbarum mainly causing root rot disease of P. notoginseng. Based on the 16S rRNA sequencing, the antagonistic bacteria were characterized into four clusters, Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetesi. The genus Bacillus was the most frequently isolated, and Bacillus siamensis (Hs02), Bacillus atrophaeus (Hs09) showed strong antagonistic activity to the three pathogens. The distribution pattern differed in soil types, genera Achromobacter, Acidovorax, Brevibacterium, Brevundimonas, Flavimonas, and Streptomyces were only found in rhizosphere of healthy plants, while Delftia, Leclercia, Brevibacillus, Microbacterium, Pantoea, Rhizobium, and Stenotrophomonas only exist in soil of diseased plant, and Acinetobacter only exist in uncultivated soil. Conclusion: The results suggest that diverse bacteria exist in the P. notoginseng rhizosphere soil, with differences in community in the same field, and antagonistic isolates may be good potential biological control agent for the notoginseng root-rot diseases caused by F. oxysporum, Fusarium solani, and Panax herbarum.
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