http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Ziqian Li,Kaying Chan,Yifei Qi,Linlin Lu,Fen Ning,Mengling Wu,Haifang Wang,Yuan Wang,Shaohui Cai,Jun Du 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3
Purpose Cancer-associated fibroblasts (CAFs) activated by cancer cells has a central role in development and malignant biological behavior in colorectal cancer (CRC). Adult fibroblasts do not express Snail, but Snail-positive fibroblasts are discovered in the stroma of malignant CRC and reported to be the key role to chemoresistance. However, the reciprocal effect of CAFs expressed Snail to chemoresistance on CRC cells and the underlying molecular mechanisms are not fully characterized. Materials and Methods Snail-overexpressed 3T3 stable cell lines were generated by lipidosome and CT26 mixed with 3T3-Snail subcutaneous transplanted CRC models were established by subcutaneous injection. Cell Counting Kit-8, flow cytometry and western blotting assays were performed, and immunohistochemistry staining was studied. The cytokines participated in chemoresistance was validated with reverse transcriptase-polymerase chain reaction and heatmap. Results Snail-expression fibroblasts are discovered in human and mouse spontaneous CRCs. Overexpression of Snail induces 3T3 fibroblasts transdifferentiation to CAFs. CT26 co-cultured with 3T3-Snail resisted the impairment from 5-fluorouracil and paclitaxel in vitro. The subcutaneous transplanted tumor models included 3T3-Snail cells develop without restrictions even after treating with 5-fluorouracil or paclitaxel. Moreover, these chemoresistant processes may be mediated by CCL1 secreted by Snail-expression fibroblasts via transforming growth factor /nuclear factor-B signaling pathways. Conclusion Taken together, Snail-expressing 3T3 fibroblasts display CAFs properties that support 5-fluorouracil and paclitaxel chemoresistance in CRC via participation of CCL1 and suggest that inhibition of the Snail-expression fibroblasts in tumor may be a useful strategy to limit chemoresistance.
Tao Liu,Jingjing Dong,Chaonan Ye,Xianyan Wu,Wei Fan,Linlin Lu,Jingyu Kang,Xingzhong Gao,Baozhong Sun,Honglei Yi 한국섬유공학회 2022 Fibers and polymers Vol.23 No.12
In this work, the bending modal properties of three-dimensional (3D) braided composites were investigated withthe experimental and numerical methods. Three braided angles were selected to study the braided angle effect on the modalbehavior of 3D braided composites. According to the experimental parameters, a homogeneous model containing the interior,surface and corner representative unit cells of the braided composites were proposed to compute the elastic constants of eachcomposite. Besides, the fiber breakage defects were introduced to the homogeneous model to improve the accuracy ofpredicting the modal behavior of the braided composite. The results indicate that the increase of the braided angle and thefiber breakage defects could apparently reduce the natural frequency of the braided composite. The modal propertiessimulated by the homogeneous models containing some fiber breakage defects could fit well with the correspondingexperimental results. The proposed method to create the homogenous model can be used to predict the modal behavior of the3D braided composites.
SNPs in the 5’ terminal-region of IGFBP6 gene and its linkage with pig body size
Xibi Fang,Songcai Liu,Yunyun Cheng,Siming Li,Qingyan Wu,Dan Su,Chao Lu,Hao Yu,Linlin Hao 한국통합생물학회 2015 Animal cells and systems Vol.19 No.6
Insulin-like growth factor binding protein 6 (IGFBP6) is a key factor in regulating the effects of insulin-like growth factor 2 (IGF2) on the animal growth and development, but the mechanism is far from known. In this study, the 5’-terminal sequence of the IGFBP6 gene (from −920 to −1 bp) which may regulate gene expression was sequenced in the Bama mini-pigs, the Tibetan mini-pigs, the Landrace pigs, the Large White pigs and the Northeast wild boars to screen for the single nucleotide polymorphisms (SNPs) and analyze the relations with the body size traits of swine through a chi-square test analysis. The genotype frequencies of the SNPs in the 5’-terminal sequence have shown that c.-726C > T, c.-722T > C, c.-535C > A, c.- 488T > C, c.-403A > G, and c.-378T > C may be related to the dwarf traits of the Bama mini-pig and the Tibetan minipigs (P < .05). A haplotype analysis of the 5’-terminal sequence of the IGFBP6 gene in the Landrace pigs, the Large White pigs and the Northeast wild boars found the two SNPs at −403 nt and −378 nt were in linkage and formed three kinds of haplotypes; AT was the dominant haplotype and the haplotype block was not formed in the Bama mini-pigs and Tibetan mini-pigs. Above all, the SNPs and haplotypes of the 5’-terminal sequence of the IGFBP6 gene may be involved in the regulation of swine body size.