H. pylori 제균 실패율과 clarithromycin 내성률의 일치성

허재형 ( J. H. Heo ),남승우 ( S. W. Nam ),노임환 ( I. H. Roe ),양미라 ( M. R. Yang ),김정택 ( J. T. Kim ),송일환 ( I. H. Song ),임창영 ( C. Y. Lim ),김정원 ( J. W. Kim ),신지현 ( J. H. Shin ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> H. pylori 제균 치료성적을 좌우하는 요소에는 약제와 대상 환자군의 선정, 균검사방법의 차이, 항생제 저항성 등이 중요시되고 있다. 이 중에서도 항생제 저항성은 나라간의 H. pylori 제균 성적을 다르게 하는 대표적인 원인이다. 우리나라는 제균률이 외국보다 저조하여 85%내외로 보고되고 있다. 본 연구의 목적은 H. pylori 제균 실패율과 clarithromycin 내성률을 조사하여 제균 실패 원인으로서의 clarithromycin

Effect of Curing Time on the Physicochemical and Sensory Properties of Beef Jerky Replaced Salt with Soy Sauce, Red Pepper Paste and Soybean Paste

Lim, H.J.,Kim, G.D.,Jung, E.Y.,Seo, H.W.,Joo, S.T.,Jin, S.K.,Yang, H.S. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.8

This study was done to investigate the quality properties of beef jerky with soy sauce, red pepper paste, and soybean paste replacing salt. Sliced beef samples were cured in salt (control), soy sauce, red pepper paste, and soybean paste for 24 or 48 h and then dried at $70^{\circ}C$ for 8 h. Treatments showed higher final moisture content and lower $Na^+$ concentration than the control after drying for 8 h. The lightness and shear force values were lower in all treatment samples than in the control during 48 h of curing time. In particular, lower lipid oxidation was found in the jerky cured with red pepper paste than in the control. Sensory evaluation showed that color, flavor, and tenderness of jerky samples were improved by replacing salt with soy sauce, red pepper paste and soybean paste, and higher likeability scores of the beef jerky were obtained among those treatments after 48 h of curing time.

Live attenuated nephropathogenic infectious bronchitis virus vaccine provides broad cross protection against new variant strains.

Lim, T-H,Kim, M-S,Jang, J-H,Lee, D-H,Park, J-K,Youn, H-N,Lee, J-B,Park, S-Y,Choi, I-S,Song, C-S Poultry Science Association, etc 2012 Poultry science Vol.91 No.1

<P>Infectious bronchitis virus (IBV) infections cause great economic losses to the poultry industry worldwide, and the emergence of new variant strains complicates disease control. The present study investigated the genetic and protectotypic features of newly emerged Korean IBV strains. A phylogenetic analysis showed that several recent isolates formed 2 different clusters (new cluster 1 and 2), which were distinct from other preexisting clusters. New cluster 1 IBV strains represented recombinants between Korean nephropathogenic strain KM91 and the QXIBV strain. New cluster 2 IBV strains showed low amino acid homology (<58.7%) compared with previous isolates. We evaluated the protective efficacy of commercial IBV vaccines (H120 and K2 strain) against these new isolates. In cross-protection studies, the H120 strain did not provide sufficient protection against these variants. However, highly attenuated nephropathogenic IBV vaccine, K2 strain, provided significantly higher levels of protection against variants compared with chickens vaccinated with H120 (P < 0.05 or better). These results indicate that the K2 vaccine could be helpful for the reduction of economic losses caused by newly evolving IBV recombinants (new cluster 1) and variants (new cluster 2).</P>

NFATc4 and ATF3 Negatively Regulate Adiponectin Gene Expression in 3T3-L1 Adipocytes

Kim, H. B.,Kong, M.,Kim, T. M.,Suh, Y. H.,Kim, W.-H.,Lim, J. H.,Song, J. H.,Jung, M. H. American Diabetes Association 2006 Diabetes Vol.55 No.5

<P>Expression of adiponectin decreases with obesity and insulin resistance. At present, the mechanisms responsible for negatively regulating adiponectin expression in adipocytes are poorly understood. In this investigation, we analyzed the effects of 5' serial deletion constructs on the murine adiponectin promoter. Here, we identified the repressor region located between -472 and -313 bp of the promoter. Removal of the putative nuclear factor of activated T-cells (NFATs) binding site increased the promoter activity, and overexpression of NFATc4 reduced the promoter activity. Treatment with the calcium ionophore A23187, an activator of NFAT, reduced mRNA as well as promoter activity. The binding of NFATc4 to the promoter was associated with increased recruitment of histone deacetylase 1 and reduced acetylation of histone H3 at the promoter site. In addition, binding of activating transcription factor 3 (ATF3) to the putative activator protein-1 site located adjacent to the NFAT binding site also repressed the promoter activity. Treatment with thapsigargin, an inducer of ATF3, reduced both mRNA and promoter activity. Importantly, the binding activities of NFATc4 and ATF3, increased significantly in white adipose tissues of ob/ob and db/db mice compared with controls. Taken together, this study demonstrates for the first time that NFATc4 and ATF3 function as negative regulators of adiponectin gene expression, which may play critical roles in downregulating adiponectin expression in obesity and type 2 diabetes.</P>

Shewanella fodinae sp. nov., isolated from a coal mine and from a marine lagoon

Sravan Kumar, R.,Sasi Jyothsna, T. S.,Sasikala, Ch.,Seong, C. N.,Lim, C. H.,Park, S. C.,Ramana, Ch. V. Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.7

<P>Strains JC15<SUP>T</SUP> and JC19 were isolated from samples collected from different locations in India, including a coal mine and a marine lagoon. Both strains were Gram-stain-negative rods, motile by means of a single polar flagellum, catalase- and oxidase-positive, and hydrolysed casein, produced H2S and showed <I>β</I>-haemolysis. Strain JC15<SUP>T</SUP> grew optimally at pH 6 (range pH 5-8) while strain JC19 grew optimally at pH 7 (range pH 6-9) and both had a growth temperature optimum of 30-37 °C (range 22-40 °C). Both strains could grow chemo-organoheterotrophically and chemolithoautotrophically. Neither strain required NaCl for growth and both could tolerate up to 9 % (w/v) NaCl, with optimum growth at 5 % NaCl. Vitamin B12 was required as a growth factor by both strains. The major fatty acids were iso-C15 : 0, C17 : 1<I>ω</I>8<I>c</I> and iso-C13 : 0 3-OH. The DNA G<I>+</I>C contents of strains JC15<SUP>T</SUP> and JC19 were 53.6 and 54.3 mol%, respectively. A phylogenetic tree based on 16S rRNA gene sequence analysis showed that strains JC15<SUP>T</SUP> and JC19 were most closely related to <I>Shewanella haliotis</I> DW01<SUP>T</SUP> (approximately 94 % sequence similarity) and to other members of the genus <I>Shewanella.</I> Genomic relatedness (DNA-DNA hybridization) between strains JC15<SUP>T</SUP> and JC19 is 88 %. On the basis of phenotypic and molecular genetic evidence, strain JC15<SUP>T</SUP> represents a novel species of the genus <I>Shewanella</I>, for which the name <I>Shewanella fodinae</I> sp. nov. is proposed. The type strain is JC15<SUP>T</SUP> (=CCUG 57102<SUP>T</SUP> =NBRC 105216<SUP>T</SUP> =KCTC 22506<SUP>T</SUP>).</P>

Association of tumor necrosis factor- gene polymorphisms with advanced stage endometriosis

Lee, G. H.,Choi, Y. M.,Kim, S. H.,Hong, M. A.,Oh, S. T.,Lim, Y. T.,Moon, S. Y. Oxford University Press 2008 Human reproduction Vol.23 No.4

<P>BACKGROUND: This study was performed to investigate whether specific haplotypes and several single nucleotide polymorphisms in the promoter region of the tumor necrosis factor (TNF)-alpha gene are associated with the risk of advanced stage endometriosis in a Korean population. METHODS: This study comprised women with (n = 246) or without (n = 248) endometriosis. The TNF:g.[-1031T > C], TNF:g.[-863C > A] and TNF:g.[-857C > T] polymorphism in the TNF-alpha gene were assessed by PCR-restriction fragment length polymorphism analysis, which utilized digestion by BbsI, HypCH4IV and HypCH4IV restriction enzymes, respectively. In silico haplotypes were deduced by using the Haploview version 3.32. RESULTS: The genotype distribution of TNF:g.[-1031T > C] was significantly different between total endometriosis patients and the controls (T/T of 56.9 versus 60.1%, T/C of 35.4 versus 37.5% and C/C of 7.7 versus 2.4%, respectively, P = 0.027). This difference at the TNF:g.[-1031T > C] tends to increase in Stage IV endometriosis (P = 0.01). However, there was no difference in the TNF:g.[-863C > A] and TNF:g.[-857C > T] site between the two groups. Even when the endometriosis cases were subdivided into American Society for Reproductive Medicine Stages III and IV, genotype differences were not found. The CC homozygote at TNF:g.-863 was more frequently found in the controls than Non-CC group (P = 0.04; odds ratio = 0.67; 95% confidence interval = 0.45-0.98). All haplotypes and diplotypes, deduced by in silico analysis, showed no association with subgroups or controls. CONCLUSIONS: Our results suggest that the genotype frequencies at the TNF:g.[-1031T > C] and the TNF:g.[-863C > A] sites may be associated with advanced stage endometriosis in the Korean population.</P>

Microstructure-dependent fatigue behavior of aged Cu-6Ni-1.5Si alloy with discontinuous/cellular precipitates

Goto, M.,Yamamoto, T.,Han, S.Z.,Lim, S.H.,Kim, S.,Iwamura, T.,Kitamura, J.,Ahn, J.-H.,Yakushiji, T.,Lee, J. Elsevier 2019 Materials science & engineering. properties, micro Vol.747 No.-

<P><B>Abstract</B></P> <P>The effect of microstructure on the fatigue strength of age-hardened Cu–6Ni–1.5Si alloy was investigated in this study. The aging was conducted at 500 °C for 0.5 h for the precipitation of disk-shaped δ-Ni<SUB>2</SUB>Si precipitates (continuous precipitates) and 3 h for the discontinuous precipitation of fiber-shaped, stable δ-Ni<SUB>2</SUB>Si precipitates to strengthen the Cu matrix. The tensile strength of 0.5 h-aged specimens was about 1.3 times greater than that of the 3 h-aged counterparts, but with inferior electrical conductivity. Despite the considerable difference in tensile strength, no notable difference in fatigue strength was observed between 0.5 h- and 3 h-aged specimens. The 3 h-aged specimens had superior electrical conductivity without sacrificing fatigue strength of the 0.5 h-aged specimens. The physical background of high-cycle fatigue strength in Cu–6Ni–1.5Si alloys with continuous precipitates and discontinuous precipitates is discussed considering the microstructure-sensitive behavior of fatigue cracks.</P>

Comparison of the Haas and the Oxford classifications for prediction of renal outcome in patients with IgA nephropathy

Park, K.S.,Han, S.H.,Kie, J.H.,Nam, K.H.,Lee, M.J.,Lim, B.J.,Kwon, Y.E.,Kim, Y.L.,An, S.Y.,Kim, C.H.,Doh, F.M.,Koo, H.M.,Oh, H.J.,Kang, S.W.,Choi, K.H.,Jeong, H.J.,Yoo, T.H. W. B. Saunders Co ; Centrum Philadelphia 2014 Human pathology Vol.45 No.2

Pathologic features can provide valuable information for determining prognosis in IgA nephropathy (IgAN). However, it is uncertain whether the Oxford classification, a new classification of IgAN, can predict renal outcome better than previous ones. We conducted a retrospective cohort study in 500 patients with biopsy-proven IgAN between January 2002 and December 2010 to compare the ability of the Haas and the Oxford classifications to predict renal outcome. Primary outcome was a doubling of the baseline serum creatinine concentration (D-SCr). During a mean follow-up of 68months, 52 (10.4%) and 35 (7.0%) developed D-SCr and end-stage renal disease, respectively. There were graded increases in the development of D-SCr in the higher Haas classes. In addition, the primary endpoint of D-SCr occurred more in patients with the Oxford M and T lesions than those without such lesions. In multivariate Cox regression analyses, the Haas class V (HR, 12.19; P=.002) and the Oxford T1 (hazard ratio [HR], 6.68; P<.001) and T2 (HR, 12.16; P<.001) lesions were independently associated with an increased risk of reaching D-SCr. Harrell's C index of each multivariate model with the Haas and the Oxford classification was 0.867 (P=.015) and 0.881 (P=.004), respectively. This was significantly higher than that of model with clinical factors only (C=0.819). However, there was no difference in C-statistics between the 2 models with the Haas and the Oxford classifications (P=.348). This study suggests that the Haas and the Oxford classifications are comparable in predicting progression of IgAN.

Effect of Co/Ni ratios in cobalt nickel mixed oxide catalysts on methane combustion

Lim, T.H.,Cho, S.J.,Yang, H.S.,Engelhard, M.,Kim, D.H. Elsevier 2015 Applied Catalysis A Vol.505 No.-

A series of cobalt nickel mixed oxide catalysts with the varying ratios of Co to Ni, prepared by co-precipitation method, were applied to methane combustion. Among the various ratios, cobalt nickel mixed oxides having the ratios of Co to Ni of (50:50) and (67:33) demonstrate the highest activity for methane combustion. Structural analysis obtained from X-ray diffraction (XRD) and extended X-ray absorption fine structure (EXAFS) evidently demonstrates that CoNi (50:50) and (67:33) samples consist of NiCo<SUB>2</SUB>O<SUB>4</SUB> and NiO phase and, more importantly, NiCo<SUB>2</SUB>O<SUB>4</SUB> spinel structure is largely distorted, which is attributed to the insertion of Ni<SUP>2+</SUP> ions into octahedral sites in Co<SUB>3</SUB>O<SUB>4</SUB> spinel structure. Such structural disorder results in the enhanced portion of surface oxygen species, thus leading to the improved reducibility of the catalysts in the low temperature region as evidenced by temperature programmed reduction by hydrogen (H<SUB>2</SUB> TPR) and X-ray photoelectron spectroscopy (XPS) O 1s results. They prove that structural disorder in cobalt nickel mixed oxides enhances the catalytic performance for methane combustion. Thus, it is concluded that a strong relationship between structural property and activity in cobalt nickel mixed oxide for methane combustion exists and, more importantly, distorted NiCo<SUB>2</SUB>O<SUB>4</SUB> spinel structure is found to be an active site for methane combustion.

H2 / SiH4 와 마이크로웨이브 출력에 따른 ECRPCVD A-Si:H 박막의 특성

강문상(M. S. Kang),이진엽(J. Y. Lee),전법주(B. J. Jeon),신동우(D. W. Sin),김재영(J. Y. Kim),임태훈(T. H. Lim),안철(C. An) 대한전자공학회 1995 대한전자공학회 학술대회 Vol.1995 No.12