The role of Jacalin-related lectin gene AOL_s00083g511 in the development and pathogenicity of the nematophagous fungus Arthrobotrys oligospora

Dong Xinyuan,Si Jiali,Zhang Guanghui,Shen Zhen,Zhang Li,Sheng Kangliang,Wang Jingmin,Kong Xiaowei,Zha Xiangdong,Wang Yongzhong 한국미생물학회 2021 The journal of microbiology Vol.59 No.8

Arthrobotrys oligospora is a model species of nematophagous fungi and has great potential for the biological control of nematode diseases. Lectin is a protein that binds to carbohydrates and their complexes with high specificity, which mediates recognition events in various physiological and pathological processes. This study aimed to investigate the role of the Jacalin-related lectin (JRL) gene, AOL_s00083g511, in A. oligospora development. Through a homology recombination approach, we obtained the AOL_s00083g511 knockout mutant strain (Δg511). Next, the biological characteristics of the Δg511 mutant strain, including growth rate, conidia germination rate, adaptation to environmental stresses, and nematocidal activity, were compared with those of the wild-type (WT) strain. The results showed that the JRL gene AOL_ s00083g511 did not affect fungal growth, conidia germination, 3D-trap formation, and the ability of A. oligospora to prey on nematodes significantly. We speculate that this phenomenon may be caused by a loss of the key β1–β2 loops in the AOL_ s00083g511-encoded JRL domain and an intrinsic genetic compensation of AOL_s00083g511 in this fungus. The growth rates of both strains on high salt or surfactant media were similar; however, in the strong oxidation medium, the growth rate of the Δg511 mutant was significantly lower than that of the WT strain, indicating that AOL_s00083g511 might play a role in oxidative stress resistance. These findings provide a basis for further analysis of the related functions of the JRL gene in A. oligospora and their potential roles in the biological control of nematodes in the future.

Photocatalytic Degradation of Rhodamine B via Fe-g-C3N4 Activated Sulfate Radical-Based Advanced Oxidation Processes and the Synergistic Mechanism

Zhan Xinyuan,She Zhixiang,Yue Zhengbo,Hu Fupeng,Wang Guangcheng,Wang Shaoping,Li Wei,Liu Bing,Wang Jin 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.1

Fe-doped g-C 3 N 4 has been proven to have the potential of visible light photocatalysis, but its catalytic activity of peroxymonosulfate (PMS) is neglected. Herein, the complex advanced oxidation processes of Fe-g-C 3 N 4 mediated PMS and visible light photocatalysis was developed, named as Vis/Fe-g-C 3 N 4 /PMS system, whose eff ects and synergistic mechanism for decomposing Rhodamine B (RhB) was evaluated. The coupling of sulfate radicals and photocatalysis for RhB degradation showed that the synergistic effi ciency ( η Syn ) and factor ( S c ) were 20.1% and 4.82, respectively, with a degradation effi ciency of 99.8%. Iron species dispersed on g-C 3 N 4 provided active sites for PMS activation to generate sulfate radicals, simultaneously reduced the forbidden band, and separated the photo-generated charges of g-C 3 N 4 . h + , SO 4 · ‾ and 1 O 2 were the main active species, and the increase of 1 O 2 was the cause of the synergistic eff ect. The possible degradation path of RhB by this coupling system was proposed. Our fi ndings prove that Vis/Fe-g-C 3 N 4 /PMS system has a great potential to decompose dye wastewater, and also to be an environmental remediation perspective.

Towards a Comprehensive Analysis of Crowds Anonymity System

Tianbo Lu,Xinyuan Zhang,Xiaofeng Du,Yang Li 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.7

There is an increasing command for anonymity in network. Crowds[1] is an popular anonymity system proposed by Michael K. Reiter and Aviel D. Rubin which main idea behind it is hiding users’ identities by routing their packets randomly within a group of similar users. Crowds can provides sender anonymity and also has an advantage that the computation load of relay nodes is very small but it does not protect the identity of the receiver. This paper presents an overview on Crowds and introduces the development of Crowds from the following aspects: anonymity analysis, application especially the application in P2P and the performance. We summarize the work about anonymity analysis based on Crowds and divide them into receiver analysis, probabilistic behavior, attack and anonymity proof. Crowds is high efficient but it can’t resist the global attack, therefore, to meet the needs of the different levels of anonymity, there is a vast body of anonymity literature concerned with anonymity analysis, for example, Crowds can’t provide receiver anonymity but receiver anonymity is important in many cases, so many work extending the Crowds system from sender anonymity only to sender and receiver anonymity. In addition to the analysis, we also summarize the applications of Crowds. Undoubtedly, it is widely used in P2P. We also give an overlook of the performance of Crowds.

F-Crowds : An Anonymity Scheme for P2P File-Sharing

Tianbo Lu,Xinyuan Zhang,Xiaofeng Du,Yang Li 보안공학연구지원센터 2016 International Journal of Security and Its Applicat Vol.10 No.6

The traditional encryption methods pay more attention to the confidentiality of the message content, but ignore the protection of identity information to the communicating parties. By encrypting, the contents of the communication acquired by attackers became relatively difficult, but they can still found the identity information of the two sides to communicate easily because in the TCP / IP protocol, the sender and receiver of the message are exposed. The anonymity communication technology, is used to solve this problem. The first anonymity communication technology can date back to 1981, Chaum proposed MIX [1] which is a very important technology for reference by a number of researchers of anonymity communication. The rapid development of the Internet has made things convenience to people, but also led to more personal information exposed to the public. As a result, a growing number of areas that required anonymity communication technology to protect the user's privacy. P2P file-sharing is not exception. P2P is undoubtedly the most popular way for file-sharing, but popular P2P file-sharing protocols, such as BitTorrent, can’t provide users anonymity communication service. Many people choose to build upon existing anonymous communication tools, such as Tor, to achieve anonymous P2P file transfer, but Tor primarily provides anonymous service for web browsing, instant message or other low latency applications. A large number of P2P file traffic will seriously threaten the service of Tor. Therefore, the anonymity communication service specifically for P2P file sharing system is very important. In this paper, we presents an anonymity communication scheme specifically for P2P file-sharing networks called F-Crowds which based on Crowds [2] to provide an adjustable anonymity service for P2P file-sharing.

Decrease of Oxygen Vacancy by Zn-Doped for Improving Solar-Blind Photoelectric Performance in β-Ga2O3 Thin Films

Daoyou Guo,Xinyuan Qin,Ming Lv,Haoze Shi,Yuanli Su,Guosheng Yao,Shunli Wang,Chaorong Li,Peigang Li,Weihua Tang 대한금속·재료학회 2017 ELECTRONIC MATERIALS LETTERS Vol.13 No.6

Highly (201) oriented Zn-doped β-Ga2O3 thin films with differentdopant concentrations were grown on (0001) sapphire substrates byradio frequency magnetron sputtering. With the increase of Zn dopantconcentration, the crystal lattice expands, the energy band gap shrinks,and the oxygen vacancy concentration decreases. Both the metalsemiconductor metal (MSM) structure photodetectors based on thepure and Zn-doped β-Ga2O3 thin films exhibit solar blind UVphotoelectric property. Compared to the pure β-Ga2O3 photodetector,the Zn-doped one exhibits a lower dark current, a higher photo/darkcurrent ratio, a faster photoresponse speed, which can be attributed tothe decreases of oxygen vacancy concentration.

Three Degrees of Freedom Global Calibration Method for Measurement Systems with Binocular Vision

Guan Xu,Xinyuan Zhang,Xiaotao Li,Jian Su,Xue Lu,Huanping Liu,Zhaobing Hao 한국광학회 2016 Current Optics and Photonics Vol.20 No.1

We develop a new method to globally calibrate the feature points that are derived from the binocularsystems at different positions. A three-DOF (degree of freedom) global calibration system is establishedto move and rotate the 3D calibration board to an arbitrary position. A three-DOF global calibration modelis constructed for the binocular systems at different positions. The three-DOF calibration model unifiesthe 3D coordinates of the feature points from different binocular systems into a unique world coordinatesystem that is determined by the initial position of the calibration board. Experiments are conducted onthe binocular systems at the coaxial and diagonal positions. The experimental root-mean-square errorsbetween the true and reconstructed 3D coordinates of the feature points are 0.573 mm, 0.520 mm and0.528 mm at the coaxial positions. The experimental root-mean-square errors between the true andreconstructed 3D coordinates of the feature points are 0.495 mm, 0.556 mm and 0.627 mm at the diagonalpositions. This method provides a global and accurate calibration to unity the measurement points ofdifferent binocular vision systems into the same world coordinate system.

Population genetics reveals new introgression in the nucleus herd of min pigs

Liu Tianxin,Ji Dongqing,Li Xinyuan,Liu Jiadong,Xu Fei,Miao Zhiying,Chang Yang,Tian Ming,Xu Chunzhu 한국유전학회 2024 Genes & Genomics Vol.46 No.4

Objective Min pigs are a unique genetic resource among local pig breeds in China. They have more excellent characteristics in cold and stress resistance, good meat quality, and a high reproductive rate. However, the genetic structure and driving factors remain unclear in the nucleus herd. In this study, the genetic diversity of Min pigs was studied to reveal the formation mechanism of its unique genetic structure. We hope to protect and develop the genetic resources of Min pigs. Methods We analyzed different types of genes to identify the genetic structure and gene introgression pattern of Min pigs. The nuclear DNA dataset includes information on 21 microsatellite loci and 6 Y-chromosome genes, and the mitochondrial D-loop gene is selected to represent maternal lineages. The above genes are all from the nucleus herd of Min pigs. Results The results of genetic structure identification and analysis of potential exogenous gene introgression patterns indicate that the nucleus herd of Min pigs maintains a high level of genetic diversity (polymorphism information content = 0.713, expected heterozygosity = 0.662, observed heterozygosity = 0.612). Compared with other Asian pig breeds, the formation of Min pig breeds is more special. Gene introgression from European pig breeds to Min pigs has occurred, which is characterized by complete introgression of paternal genes and incomplete introgression of maternal genes. Conclusion Gene introgression caused by cross-breeding is not the main factor leading to the formation of the current genetic structure of Min pigs, but this process has increased the level of genetic diversity in the nucleus herd. Compared with the influence of gene introgression, our research suggest that artificial selection and environmental adaptive evolution make Min pigs form unique genetic characteristics. Objective Min pigs are a unique genetic resource among local pig breeds in China. They have more excellent characteristics in cold and stress resistance, good meat quality, and a high reproductive rate. However, the genetic structure and driving factors remain unclear in the nucleus herd. In this study, the genetic diversity of Min pigs was studied to reveal the formation mechanism of its unique genetic structure. We hope to protect and develop the genetic resources of Min pigs. Methods We analyzed different types of genes to identify the genetic structure and gene introgression pattern of Min pigs. The nuclear DNA dataset includes information on 21 microsatellite loci and 6 Y-chromosome genes, and the mitochondrial D-loop gene is selected to represent maternal lineages. The above genes are all from the nucleus herd of Min pigs. Results The results of genetic structure identification and analysis of potential exogenous gene introgression patterns indicate that the nucleus herd of Min pigs maintains a high level of genetic diversity (polymorphism information content = 0.713, expected heterozygosity = 0.662, observed heterozygosity = 0.612). Compared with other Asian pig breeds, the formation of Min pig breeds is more special. Gene introgression from European pig breeds to Min pigs has occurred, which is characterized by complete introgression of paternal genes and incomplete introgression of maternal genes. Conclusion Gene introgression caused by cross-breeding is not the main factor leading to the formation of the current genetic structure of Min pigs, but this process has increased the level of genetic diversity in the nucleus herd. Compared with the influence of gene introgression, our research suggest that artificial selection and environmental adaptive evolution make Min pigs form unique genetic characteristics.

Manufacturing Principle and Meshing Limit Line of Toroidal Surface Enveloping Cylindrical Worm Drive with Arc-Toothed Worm

Xinyue Zhu,Yaping Zhao,Yifei Chi,Gongfa Li,Xinyuan Chen 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.23 No.3

The main purpose of this article is to determine the processing parameters of the worm and establish the theory of the meshing limit line of toroidal surface enveloping cylindrical worm drive with the arc-toothed worm. Based on this condition that the instantaneous contact line is a planar curve during grinding the worm blank, it can be deduced that its torsion is equal to zero, then the formulae which determine the installing position of the grinding wheel are acquired. Following this, the equations of the tooth surfaces and the meshing limit function are all obtained. According to the geometric construction and elimination method, the meshing limit points are determined by iteratively solving the corresponding nonlinear equation in its solution domain. In addition, the result of the numerical case demonstrates that the worm helicoid is divided into two parts by the meshing limit line, which makes the working length of the worm cannot reach half of its total thread length. And by adjusting the design parameters of the worm pair and the processing parameters of the worm, the meshing limit line cannot be eliminated and its position on the surfaces of the worm and the worm gear has no notable change.

Case(1)of Chinese northern area

Jong-Ho Shin,Chengjun Gao,Xinyuan Zhang,Xiao Ping Li 한국펄프·종이공학회 2003 펄프종이기술국제세미나 Vol.29 No.-

Multifunctional hydrogel delivery system for disease therapy

Weihong Mao,Qing Ji,Ping Chen,Zhengzou Fang,Xinyuan Li 한국고분자학회 2023 Macromolecular Research Vol.31 No.4

Hydrogel have been widely applied in the field of tissue engineering and disease therapy, because hydrogel can not only regenerate and repair damaged tissues, but also avoid unnecessary side effects as well as enhance the accumulation at lesions. The function of hydrogel mainly depended on the property of basic polymer materials. The nature-like tissue and environment-friendly characteristics of hydrogel are widely applied in disease therapy. Therefore, hydrogel was carefully designed from these biocompatible polymer materials through covalent and noncovalent crosslinking, and can be recognized as suitable scaffolds for tissue repair and regeneration. The obtained hydrogel can promote the transport of nutrients and oxygen through interconnected macropores. In addition, the physical and chemical crosslinking versatility of hydrogel can not only enhance its mechanical strength and adhesive property, but also can be used as a reservoir to realize sustain-release of drugs. Moreover, hydrogel can fill the irregular lesions sites, promote wound healing and bear the high-frequency motion of the lesion sites. In this paper, we will summarize some biocompatible hydrogels for some primary disease treatment, and provide some inspiration for researchers to design suitable hydrogel, so as to overcome the limitations of current disease treatment and promote the clinical transformation of hydrogel.