MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Zhang, Lei-Lei,Wu, Jiang,Liu, Qiang,Zhang, Yan,Sun, Zhu-Lei,Jing, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

Melatonin Attenuates Noise Stress-induced Gastrointestinal Motility Disorder and Gastric Stress Ulcer: Role of Gastrointestinal Hormones and Oxidative Stress in Rats

( Lei Zhang ),( Ji T Gong ),( Hu Q Zhang ),( Quan H Song ),( Guang H Xu ),( Lei Cai ),( Xiao D Tang ),( Hai F Zhang ),( Fang E Liu ),( Zhan S Jia ),( Hong W Zhang ) 대한소화기기능성질환·운동학회 2015 Journal of Neurogastroenterology and Motility (JNM Vol.21 No.2

Background/Aims There are increasing evidences for gastrointestinal motility disorder (GIMD) and gastric stress ulcer induced by noise stress. The present study was to investigate the reversed effect of melatonin on GIMD and gastric stress ulcer induced by noise stress and potential mechanism. Methods Noise stress was induced on rats, and melatonin (15 mg/kg) was administered to rats by intraperitoneal injection. Differences were assessed in gastric residual rate (GRR), small intestine propulsion rate (SPR), Guth injury score, cortisol, gastrointestinal hormones (calcitonin-gene-related peptide and motilin) and oxidative stress markers (superoxide dismutase and malondialde hyde) in blood plasma as well as gastric mucosa homogenate with or without melatonin. The pathological examination of gastric mucosa was also performed. Results The GRR and SPR were improved by noise stress compared with control (P < 0.05). The pathological examination and Guth injury score revealed gastric stress ulcer. Moreover, the levels of cortisol, motilin and malondialdehyde in blood plasma and malondialdehyde in gastric mucosa homogenate were increased by noise stress (P < 0.05). CGRP and superoxide dismutase activity in both of blood plasma and gastric mucosa homogenate were significantly decreased (P< 0.05). Furthermore, melatonin reversed changes in GRR, SPR, pathological examination, Guth injury score, cortisol, motilin, CGRP, superoxide dismutase activity and malondialdehyde (P < 0.05). Conclusions Melatonin is effective in reversing the GIMD and gastric stress ulcer induced by noise stress. The underlying mechanism may be involved in oxidative stress and gastrointestinal hormones. (J Neurogastroenterol Motil 2015;21:189-199)

Chinese Society of Allergy Guidelines for Diagnosis and Treatment of Allergic Rhinitis

Lei Cheng,Jianjun Chen,Qingling Fu,Shaoheng He,Huabin Li,Zheng Liu,Guolin Tan,Zezhang Tao,Dehui Wang,Weiping Wen,Rui Xu,Yu Xu,Qintai Yang,Chonghua Zhang,Gehua Zhang,Ruxin Zhang,Yuan Zhang,Bing Zhou,Do 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

Allergic rhinitis (AR) is a global health problem that causes major illnesses and disabilities worldwide. Epidemiologic studies have demonstrated that the prevalence of AR has increased progressively over the last few decades in more developed countries and currently affects up to 40% of the population worldwide. Likewise, a rising trend of AR has also been observed over the last 2-3 decades in developing countries including China, with the prevalence of AR varying widely in these countries. A survey of self-reported AR over a 6-year period in the general Chinese adult population reported that the standardized prevalence of adult AR increased from 11.1% in 2005 to 17.6% in 2011. An increasing number of original articles and imporclinical trials on the epidemiology, pathophysiologic mechanisms, diagnosis, management and comorbidities of AR in Chinese subjects have been published in international peer-reviewed journals over the past 2 decades, and substantially added to our understanding of this disease as a global problem. Although guidelines for the diagnosis and treatment of AR in Chinese subjects have also been published, they have not been translated into English and therefore not generally accessible for reference to non-Chinese speaking international medical communities. Moreover, methods for the diagnosis and treatment of AR in China have not been standardized entirely and some patients are still treated according to regional preferences. Thus, the present guidelines have been developed by the Chinese Society of Allergy to be accessible to both national and international medical communities involved in the management of AR patients. These guidelines have been prepared in line with existing international guidelines to provide evidence-based recommendations for the diagnosis and management of AR in China.

Anomalous magnetization jumps in granular Pb superconducting films

Zhang An-Lei,Jiang Wan-Yan,Chen Xing-Hong,Zhang Xiao-Ke,Lu Wen-Lai,Chen Fei,Feng Zhen-Jie,Cao Shi-Xun,Zhang Jin-Cang,Ge Jun-Yi 한국물리학회 2022 Current Applied Physics Vol.35 No.-

In granular superconductors, the grain boundaries are closely related to the vortex dynamics and the macroscopic superconducting properties. In our research, Pb films with different grain sizes were prepared by tuning the substrate temperature. With the grain size decreasing, Pb films are prone to feature the anomalous magnetization jumps in the M − T curves, while in the M − H curves flux avalanche happens. Both phenomena appear in the same region of the H − T phase diagram and thus are considered to have the same origin. The further theoretical analysis shows that with grain size decreasing the pinning mechanism evolves from a mixed δTc and δl pinning to the δl pinning mechanism. The results shed light on the study of pinning mechanism for granular superconductors and is beneficial to the potential application of manipulating vortex pinning by regulation of intrinsic defects.

Preparation and Characterization of a Novel Hyperbranched Poly(aryl ether ketone) Terminated with Cobalt Phthalocyanine to be Used for Oxidative Decomposition of 2,4,6-Trichlorophenol

Lei Lei,Yunhe Zhang,Xiujie Li,Jianxin Mu,Guibin Wang,Zhenhua Jiang,Shuling Zhang 한국고분자학회 2010 Macromolecular Research Vol.18 No.4

A novel hyperbranched poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-HPAEK)was prepared using 1,3,5-tris[4-(4-fluorobenzoyl) phenoxy] benzene (B3), as a ‘core’ molecule, 4,4'-(hexafluoroisopropylidene)diphenol (6F-BPA) as an A2 monomer, and cobalt phthalocyanine as an end-capping reagent. CoPc-THPAEK showed good solubility, thermal stability and catalytic activity in the oxidative decomposition of 2,4,6-trichlorophenol (TCP) under H2O2 or KHSO5. Moreover, the oxidative effect of KHSO5 was superior to that of H2O2,and the catalytic activity of CoPc-T-HPAEK was much higher than that of linear poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-LPAEK) and linear poly(aryl ether ketone) with a cobalt phthalocyanine pendant unit (CoPc-S-LPAEK).

Association between Polymorphisms of MSTN and MYF5 Genes and Growth Traits in Three Chinese Cattle Breeds

Zhang, R.F.,Chen, H.,Lei, C.Z.,Zhang, C.L.,Lan, X.Y.,Zhang, Y.D.,Zhang, H.J.,Bao, B.,Niu, H.,Wang, X.Z. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.12

The objective of this study was to assess the association of polymorphisms in MSTN and MYF5 genes with growth traits in three Chinese cattle breeds. Only one homozygous animal with BB genotype at MSTN locus was observed in Jiaxian population which was at Hardy-Weinberg disequilibrium (p<0.05). The frequencies of allele A at MSTN locus and allele B at MYF5 locus in the three Chinese breeds were 0.9550/0.9730/0.9720 and 0.8275/0.7581/0.7523, respectively. Allele A at MSTN locus and allele B at MYF5 locus were dominant in these three populations. No statistically significant differences in growth traits were observed between the genotypes of the Jiaxian breed at MSTN and MYF5 loci and the Nanyang breed at MYF5 locus. However, there were statistically significant differences between the genotypes at MSTN locus of the Nanyang breed for WH, HG, HGI and HGBLR (p<0.05), and of the Qinchuan breed for BLI (p<0.05). The SNP in MYF5 had significant effects on WH and HHC of Qinchuan animals (p<0.05). These results suggest that MSTN and MYF5 are strong candidate genes that influence growth traits in cattle. Other SNPs of MSTN and MYF5 or other linked genes should also be studied, which could lead to the development of selection plans to improve the performance of Chinese cattle and also promote the breeding of genuine beef cattle in China.

A Multi-objective Two-sided Disassembly Line Balancing Optimization Based on Artificial Bee Colony Algorithm: A Case Study of an Automotive Engine

Lei Zhang,Yuanfeng Wu,Xikun Zhao,Shiwen Pan,Ziqi Li,Hong Bao,Yongtin Tian 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.5

Disassembly is an important part of green manufacturing and remanufacturing. The disassembly line is an optimum form for mass and automatic disassembly in the industry. To optimize the disassembly system and the use of resources, the disassembly line balancing problem (DLBP) has attracted much attention. Compared with the conventional one-sided straight disassembly line, the two-sided disassembly line can use both the left and right sides of a conveyor belt for disassembly operation, thereby improving production efficiency, especially for large-sized and complicated products. On the other hand, due to the constraints and precedence among parts, it is a challenge to plan the disassembly scheme for a two-sided disassembly line. In this paper, a model is established to solve a two-sided disassembly line balancing problem (TDLBP). First, a hybrid graph is utilized to express constraints and precedence relationships, and a novel encoding and decoding method is developed for the disassembly scheme planning of a two-sided line for handling the challenge caused by constraints and precedence among parts. Then, a multi-objective TDLBP optimization model is proposed including the number of matedworkstations, idle time, smoothness index, the auxiliary indicator, and a meta-heuristic based on an artificial bee colony (ABC) algorithm is designed to solve TDLBP. Finally, the proposed model and method are applied to an automotive engine case, and the results are compared with NSGA-II, hybrid artificial bee colony algorithm (HABC), and multi-objective flower pollination algorithm (MOFPA) to verify the practicality of the proposed model in solving the TDLBP.

A New Unsymmetrical Zinc Phthalocyanine as Photosensitizers for Dye-sensitized Solar Cells

Zhang, Dan,Zhang, Xue-Jun,Zhang, Lei,Mao, Li-Jun Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.4

A new unsymmetrical zinc phthalocyanine has been designed and synthesized based on the 'push-pull' and extended ${\pi}$-conjugation concept for the dye-sensitized solar cells. Three tert-butoxy groups, which act as electron releasing ('push'), enhance the solubility of phthalocyanine in common organic solvents and reduce the aggregation. Hydroxy substituted 9,10-anthraquinones act as electron acceptors ('pull') for the study of photoinduced electron transfer processes as well as grafting onto nanocrystalline $TiO_2$. The new unsymmetrical zinc phthalocyanine was fully characterized by FTIR, UV-vis, $^1H$ NMR, cyclic voltammetry and differential pulse voltammetry. The new sensitizer was tested in dye-sensitized solar cells, and gave a better performance.

Folate-decorated Polysaccharide-doxorubicin Polymer: Synthesis, Characterization, and Activity in HeLa Cells

Lei Zhang,Lulu Guo,Jingna Ding,Yongming Lu,Yaping Zhang,Yan Chen 대한화학회 2015 Bulletin of the Korean Chemical Society Vol.36 No.8

In order to develop the application of the natural polysaccharide lentinan (LNT) and decrease the side effects of doxorubicin, we successfully synthesized a novel folate-modified maleilated lentinan-doxorubicin (FA-M.LNT-DOX) polymer and used it for tumor-targeted drug delivery. The release efficiency and cytotoxicity of the prodrugs were evaluated in vitro. Although DOX release from FA-M.LNT-DOX was quite slow in a neutral buffer, it was particularly fast in an acidic solution with a pH of 5.0. Compared with DOX, FA-M.LNT-DOX showed higher cytotoxicity in HeLa cells and significantly lower cytotoxicity in normal cells. These results suggested that FA-M.LNT-DOX could be considered as a potential drug delivery candidate for folate receptor-positive cancer therapy.

Effect of basicity and B₂O₃ on the viscosity and structure of fluorine-free mold flux

Zhang, Lei,Wang, Wanlin,Xie, Senlin,Zhang, Kaixuan,Sohn, Il Elsevier 2017 Journal of non-crystalline solids Vol.460 No.-

<P><B>Abstract</B></P> <P>The effect of basicity (weight ratio of CaO/SiO<SUB>2</SUB>) and B<SUB>2</SUB>O<SUB>3</SUB> on the viscosity and structure of Fluorine-free mold flux for the casting of medium carbon steels was conducted in this article, through the rotating cylinder method combined with the Fourier transform infrared (FTIR) spectroscopy. The results showed that, with the increase of basicity, the viscosity of mold flux was attenuated dramatically, and then kept constant or slight increased in the low temperature region. The reason could be explained as the degree of polymerization (DOP) of the network structure was first reduced significantly with the addition of basicity, and then the further depolymerization is less pronounced with the further increase of basicity. Beside the formation of high melting point substance leads to the slight increase of viscosity. Moreover, it suggested that the viscosity of mold flux is decreased with the addition of B<SUB>2</SUB>O<SUB>3</SUB> content, due to the fact that B<SUB>2</SUB>O<SUB>3</SUB> is a low melting point oxide which could substantially lower the break temperature of mold flux. The result of FTIR indicated B<SUB>2</SUB>O<SUB>3</SUB> acts as network former, and tends to form [BO<SUB>3</SUB>]-trihedral and [BO<SUB>4</SUB>]-tetrahedral structural units, which would connect with each other to form some simple network structure such as diborate or pentaborate. With the addition of B<SUB>2</SUB>O<SUB>3</SUB>, the free oxygen ions (O<SUP>2−</SUP>) would depolymerize the diborate structural unit, and the depolymerized diborate would link again with free [BO<SUB>3</SUB>]-trihedral to form complex pentaborate groups. Moreover, the effect of above addition on the apparent activation energy for viscous flow and break temperature of mold flux also were discussed. The results obtained in this paper provide the detailed study of the structure evolution of Fluorine-free mold flux when B<SUB>2</SUB>O<SUB>3</SUB> is added.</P>